598 research outputs found
Supplemental_Table_1 - Cost and Scalability Analysis of Porcine Islet Isolation for Islet Transplantation: Comparison of Juvenile, Neonatal and Adult Pigs
No full textSupplemental_Table_1 for Cost and Scalability Analysis of Porcine Islet Isolation for Islet Transplantation: Comparison of Juvenile, Neonatal and Adult Pigs by Rachel Vanderschelden, Mayilone Sathialingam, Michael Alexander and Jonathan R. T. Lakey in Cell Transplantation</p
Fiction is Truth: Novels as a Source for (Paradoxical) Theology
No full textWard Lecture by Carol Lakey Hess, October 22, 2009. Digital audio recording (mp3). Duration: 1 hour.
"The art of fiction tells truth because it is the truth of life that goes into making good fiction: love, hate, fear, courage, delight, sorrow, betrayal, loyalty, confusion, choice, circumstance, luck, injustice. These essential qualities, says the author, are also the qualities of sound theology, with a sense of time and place; and raising relevant questions without offering absolute answers." -- from the abstract of the author's similarly titled periodical article
Pengaruh Variasi Menu, Suasana Cafe Dan Service Quality Terhadap Kepuasan Konsumen Pada Cafe Fatma Lakey Dompu
No full textPenelitian ini bertujuan untuk menganalisis pengaruh variasi menu, suasana café, dan service quality terhadap kepuasan konsumen pada Café Fatma Lakey Dompu. Metode penelitian yang digunakan adalah pendekatan kuantitatif dengan teknik pengumpulan data melalui kuesioner, observasi, dan dokumentasi. Sampel dalam penelitian ini berjumlah 90 responden yang dipilih menggunakan teknik accidental sampling. Hasil analisis data menunjukkan bahwa secara parsial, variabel service quality berpengaruh signifikan terhadap kepuasan konsumen, sedangkan variasi menu dan suasana café tidak berpengaruh signifikan. Namun, secara simultan ketiga variabel tersebut berpengaruh signifikan terhadap kepuasan konsumen. Koefisien determinasi (R²) sebesar 41,7% menunjukkan bahwa kepuasan konsumen dapat dijelaskan oleh variasi menu, suasana café, dan service quality, sementara sisanya dipengaruhi oleh faktor lain. Temuan ini menegaskan pentingnya peningkatan kualitas pelayanan sebagai faktor utama dalam membangun kepuasan dan loyalitas pelanggan pada Café Fatma Lakey Dompu.
Kata Kunci: Variasi Menu, Suasana Café, Service Quality, Kepuasan Konsumen
Can Ordinary Conversations Account for Perceived Support
No full textPerceived social support is important because low support is linked to poor mental health, including major mental disorders, and within the normal range, low positive and high negative affect (Lakey & Orehek, 2011; PsyRev). Most social support research is guided by stress and coping theory and predicts that enacted support (e.g., advice or reassurance) protects people from the bad effects of stress (stress buffering) (Cohen & Wills, 1985; PsyBul). However, this theory does not fit the data well on certain points. For example, perceived support is most commonly linked to mental health regardless of stress (main effects), is not closely linked to enacted support, and enacted support has not been able to explain perceived supports link to mental health. Relational regulation theory (RRT; Lakey & Orehek, 2011) predicts that the main effect between perceived support and mental health reflects social regulation through ordinary conversation and shared activities. RRT also predicts that the support providers, conversations and activities that regulate a specific recipient is largely a matter of the recipients personal tastes (i.e., is relational). 100 Marine Corp reservists participated who were about to be deployed to Afghanistan. Marines rated each other in naturally-occurring 4-person teams on perceived supportiveness, conversation quality and positive as well as negative affect elicited. Provider supportiveness was rated with the Quality of Relationships Inventory (Pierce et al., 1991; JPSP), recipients affect when with the provider was assessed with the PANAS (Watson et al., 1988; JPSP), and conversation quality was assessed with the Perceived Conversation Quality Scale (Lakey et al., 2014; UndrRev). This was a round robin design to isolate relationship, recipient and provider effects (Kenny, 1994; IntpPerc). Recipient (aka actor) effects reflect the extent to which some recipients see the same providers as more supportive than other recipients. Provider (aka partner) effects reflect the extent to which recipients agree that some providers are more supportive than others. Relationship effects indicate the extent to which a recipient sees a provider as more supportive than 1) the recipients tendency to see all providers as supportive, and 2) the providers tendency to be seen as supportive by other recipients. RRT makes predictions about relationship effects specifically. The findings were as predicted by RRT. 54% of the variance in perceived support and 67% of the variance in conversation quality was relational. Relational perceived support was strongly correlated with conversational quality (r=.70). That is, when a recipient saw a provider as eliciting unusually good conversation, the recipient saw the provider as unusually supportive. In addition, relational conversation quality and perceived support were linked to high positive affect (r= .54 & .54) and low negative affect (r= -.35 & -.44). That is, when a recipient saw a provider as unusually supportive, or as eliciting unusually good conversation, the provider elicited unusually high positive and low negative affect in the recipient. Finally, multiple regression indicated that most of the link between relational support and affect overlapped with conversation quality
Biohybrid Nanoparticles to Negotiate with Biological Barriers
No full textIncapability of effective cross-talk with biological environments has partly impaired the in vivo functionality of nanoparticles (NPs). Homing, biodistribution, and function of NPs could be engineered through regulating their interactions with in vivo niches. Inspired by communications in biological systems, endowing a "biological identity" to synthetic NPs is one approach to control their biodistribution, and immunonegotiation profiles. This synthetic-biological combination is referred to as biohybrid NPs, which comprise both i) engineerable, readily producible, and trackable synthetic NPs as well as ii) biological moieties with the capability to cross-talk with immunological barriers. Here, the latest understanding on the in vivo interactions of NPs, biological barriers they face, and emerging methods for quantitative measurements of NPs' biodistribution are reviewed. Some key biomolecules that have emerged as negotiators with the immune system in the context of cancer and autoimmunity, and their inspirations on biohybrid NPs are introduced. Critical design considerations for efficient cross-talk between NPs and innate and adaptive immunity followed by hybridization methods are also discussed. Finally, clinical translation challenges and future perspectives regarding biohybrid NPs are discussed
PH dependence of listeriolysin O aggregation and pore-forming ability
No full textListeriolysin O (LLO) is the major factor implicated in the escape of Listeria monocytogenes from the phagolysosome. It is the only representative of cholesterol-dependent cytolysins that exhibits pH-dependent activity. Despite intense studies of LLO pH-dependence, this feature of the toxin still remains incompletely explained. Here we used fluorescence and CD spectroscopy to show that the structure of LLO is not detectably affected by pH at room temperature. We observed slightly altered haemolytic and permeabilizing activities at different pH values, which we relate to reduced binding of LLO to the lipid membranes. However, alkaline pH and elevated temperatures caused rapid denaturation of LLO. Aggregates of the toxin were able to bind Congo red and Thioflavin T dyes and were visible under transmission electron microscopy as large, amorphous, micrometer-sized assemblies. The aggregates had the biophysical properties of amyloid. Analytical ultracentrifugation indicated dimerization of the protein in acidic conditions, which protects the protein against premature denaturation in the phagolysosome, where toxin activity takes place. We therefore suggest that LLO spontaneously aggregates at the neutral pH found in the host cell cytosol and that this is a major mechanism of LLO inactivation. © 2011 FEBS
Margaret Lakey Collection
No full textPhotograph of L to R: Leigh, David Albert, Tom, Susie, and Roy, the son of Carl Albert's friend
Margaret Lakey Collection
No full textPhotograph of L to R: Mrs. Albert, David Albert, Congressman Carl Albert, and Mary Frances Albert
Margaret Lakey Collection
No full textPhotograph of L to R: 1. Congressman Carl Albert Greeting Juan Carlos Cordini. Inauguration of Argentine President Dr. Arturo U. Illia. Buenos Aires, Argentina, October 10, 1963
Margaret Lakey Collection
No full textPhotograph of L to R: 1. Charles Wellman, 2. Gary Clemente, 3. Gov. Edmund C. Brown, California, 4. Rep. Carl Albert, 5. Congressman William T. Cahill, New Jersey; 6. Joseph A. Beirne, Pres. Communications Workers Of America; 7. Dr. Benjamin Spock, 8. Edwin M. Martin, Under Secretary For Latin American Affairs; 9. Ralph Dungan, Special Advisor to President Kennedy; 10. Robert M. Mcclintock, U.S. Ambassador to Argentina, Buenos Aires, Argentina, October 1963
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