3,082 research outputs found
Suppression of the Pth(Ts) phenotype mediated by the overproduction of tRNA maintains moderate levels of the Pth(Ts) protein
No full text<p><b>Copyright information:</b></p><p>Taken from "Excess of charged tRNA maintains low levels of peptidyl-tRNA hydrolase in (Ts) mutants at a non-permissive temperature"</p><p>Nucleic Acids Research 2006;34(5):1564-1570.</p><p>Published online 15 Mar 2006</p><p>PMCID:PMC1408313.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> () Depicts the cellular growth of the (Ts) mutant strain AA7852 separately transformed with pVH124 (ΔU, ΔV), pVH125 (U, ΔV) or pVH119 (U, V) incubated at different temperatures. Isolated colonies of the independent transformants were streaked onto LB-Ap plates and incubated overnight at the indicated temperatures. () Presents the immunodetection of Pth(Ts) in the (Ts) mutant strain AA7852 separately transformed with pVH124, pVH125, pVH119, ptRNACCA (X, R, T, M) or pTH2 (W) and grown at 32°C prior to transfer at time = 0 min at 41 or 43°C. The concentration of Pth(Ts) protein was estimated by immunoblot analysis. The left lane shows purified wild-type Pth protein, which migrates slightly faster in SDS–PAGE than the Pth(Ts) variant (arrowed) ()
Emergence of oblique TS mode due to longitudinal wall oscillation in 2D channel flow
No full textStabilizing effects of wall oscillation on two dimensional (2D) Tollmein-Schlichting (TS) waves and oblique TS waves developing in 2D channel flow are numerically investigated using the Floquet method. It was shown from the previous study[1] that the wall oscillation mostly has stabilizing effect and then TS mode is occasionally more stable than the oblique mode. In the present study the characteristics of the stability are investigated in detail. Then it is cleared that the exchange of the most unstable mode form the 2D TS to the oblique TS can occur at a certain value of Reynolds number
Indices of Defective Autophagy in Whole Muscle and Lysosome Enriched Fractions From Aged D2-mdx Mice
No full textDuchenne muscular dystrophy (DMD) is a fatal, progressive muscle disease caused by the absence of functional dystrophin protein. Previous studies in mdx mice, a common DMD model, identified impaired autophagy with lysosomal insufficiency and impaired autophagosomal degradation as consequences of dystrophin deficiency. Thus, we hypothesized that lysosomal abundance would be decreased and degradation of autophagosomes would be impaired in muscles of D2-mdx mice. To test this hypothesis, diaphragm and gastrocnemius muscles from 11 month-old D2-mdx and DBA/2J (healthy) mice were collected. Whole muscle protein from diaphragm and gastrocnemius muscles, and protein from a cytosolic fraction (CF) and a lysosome-enriched fraction (LEF) from gastrocnemius muscles, were isolated and used for western blotting. Initiation of autophagy was not robustly activated in whole muscle protein from diaphragm and gastrocnemius, however, autophagosome formation markers were elevated in dystrophic muscles. Autophagosome degradation was impaired in D2-mdx diaphragms but appeared to be maintained in gastrocnemius muscles. To better understand this muscle-specific distinction, we investigated autophagic signaling in CFs and LEFs from gastrocnemius muscles. Within the LEF we discovered that the degradation of autophagosomes was similar between groups. Further, our data suggest an expanded, though impaired, lysosomal pool in dystrophic muscle. Notably, these data indicate a degree of muscle specificity as well as model specificity with regard to autophagic dysfunction in dystrophic muscles. Stimulation of autophagy in dystrophic muscles may hold promise for DMD patients as a potential therapeutic, however, it will be critical to choose the appropriate model and muscles that most closely recapitulate findings from human patients to further develop these therapeutics.This article is published as Krishna S, Spaulding HR, Quindry TS, Hudson MB, Quindry JC and Selsby JT (2021) Indices of Defective Autophagy in Whole Muscle and Lysosome Enriched Fractions From Aged D2-mdx Mice. Front. Physiol. 12:691245. doi: 10.3389/fphys.2021.691245.This work was funded in part by the Michael’s Cause and Ryan’s Quest (JS and JQ) as well as Parent Project Muscular Dystrophy (JS and JQ)
A TS uszályhíd kifejlesztése és alkalmazása
No full textThe essay focuses on the history of floating bridges and their special situation in Hungary. It produces the main elements and building procedures of the TS barge bridge. The author writes an appreciation of the TS barge bridge designers.The essay focuses on the history of floating bridges and their special situation in Hungary. It produces the main elements and building procedures of the TS barge bridge. The author writes an appreciation of the TS barge bridge designers
SEASONAL CHANGES IN CARDIOVASCULAR FUNCTION, RISK FACTORS, AND OXIDATIVE STRESS OF WILDLAND FIREFIGHTERS
No full textC.M. Williamson-Reisdorph, K.S. Christison, S.C. Gurney, K.G.S. Tiemessen, J.A. Sol, T.S. Quindry, C.G. Palmer,
M.W. Bundle, C.L. Dumke, FACSM, J.C. Quindry, FACSM
University of Montana, Missoula, MT
Wildland firefighters (WLFF) experience extreme physiological strain throughout a typical season due to intense occupational demands and consistent woodsmoke exposure. There is a rationale to indicate that accumulated physiological strain, and oxidative stress, throughout a WLFF season has the potential to negatively alter cardiovascular function and risk factors. PURPOSE: The purpose of the study was to examine the effects of a season on cardiovascular function, risk factors, and markers of oxidative stress in WLFF. METHODS: Fourteen members of a Type I interagency hotshot crew participated in the study (males: n=13, females: n=1, age: 30.1 years ± 4.8). Pre- and post-seasonal resting measurements (May, October) were obtained for heart rate variability (lnRMSSD, lnHF, lnHF, LF:HF), pulse wave velocity (PWV), blood lipid panels (TC, TG, LDL, HDL), metabolic biomarkers (blood glucose, HbA1c), blood pressure (SBP, DBP) and blood oxidative stress (3-nitrotyrosine, 8-isoprostane, lipid hydroperoxides). Paired samples t-tests were used to identify differences among pre- and post- seasonal values. RESULTS: There were no seasonal effects observed on resting heart rate variability, PWV, 3-nitrotyrosine, 8-isoprostane, TC, TG, LDL, blood glucose, SBP, or DBP (p\u3e0.05). A significant reduction occurred in HDL (Pre: 53 mg/dL ± 14, Post: 45 mg/dL ± 18, p=0.043) and both HbA1c (Pre: 5.2% ± 0.2 , Post: 5.3% ± 0.2, p=0.034) and lipid hydroperoxides (Pre: 2.5 ± 0.5, Post: 4.9 ± 0.6, p = 0.003 ) increased from pre- to post-season. CONCLUSION: These data suggest a WLFF season did not impact resting markers of heart rate variability, pulse wave velocity, 3-nitrotyrosine, and 8-isoprostane. Alterations in metabolic biomarkers of cardiovascular risk factors (HDL and HbA1c) and lipid hydroperoxides demonstrate unfavorable seasonal changes, suggesting that the WLFF season may increase cardiovascular risk.
Funded by the USFS 16-CR-11138200-005
The new CEN/TS 19100: Design of glass structures
No full textSince the beginning of 2021, CEN/TS 19100 Design of Glass Structures has been available in its first three parts. The fourth part is expected soon. This Technical Specification of the European standards organisation CEN is as a pre-standard of a corresponding future Eurocode. These documents constitute the first ever comprehensive design code for the entire structural glass engineering field on the European market for the first time. In addition to a clear outline, the Technical Specification has been drafted to be compatible with EN 1990 “Basis of Design” and to address glass-specific design matters, particularly related to robustness and redundancy. Although the standard still has the status of a CEN/TS, thereby allowing the European nations the option of whether to introduce it, either in full or in parts, it already contains national openings through which the European countries can adapt the design results to their own safety level by National Determined Parameters (NDPs). Such an approach already anticipates the future Eurocode, which is expected to be published as EN 19100—Design of Glass Structures. This article provides some context on the history and concept behind the new documents and gives an overview of the design rules and the corresponding technical background of the different parts of CEN/TS 19100.Applied MechanicsArchitectural Technolog
METABOLIC AND CARDIOVASCULAR ALTERATIONS DURING CRITICAL TRAINING IN WILDLAND FIREFIGHTERS
No full textS.C. Gurney, K.S. Christison, C.M. Williamson-Reisdorph, K.G.S. Tiemessen, J.A. Sol, T.S. Quindry, M.W. Bundle, C.G. Palmer, J.C. Quindry, FACSM, C.L. Dumke, FACSM
University of Montana, Missoula, MT
Wildland firefighters (WLFF) are confronted with numerous physical and mental stressors. Pre-fire season includes an intense 2- week critical training (CT) period; a preparatory phase that can result in injury, illness, and rhabdomyolysis. PURPOSE: To identify physiologic changes in metabolic biomarkers that occur during 2 weeks of CT in WLFF. METHODS: Eighteen male (29.4±1.1 yr, 182.1±1.6 cm) and three female (26.7±2.6 yr, 169.5±4.2 cm) participants were recruited from a Type I interagency hotshot fire crew and monitored over their 2-week CT. Fitness was assessed via BLM Fitness Challenge (push-ups, pull- ups, sit-ups, 1.5 mi run). Subjects were asked to fast and abstain from caffeine for blood draws on days 1, 4, 8, and 11. Plasma was analyzed for changes in the metabolic profile and oxidative stress markers 3-Nitrotyrosine (3NT), 8-Isoprostane (8ISO), and Lipid Hydroperoxides (LOOH). A one-way repeated measures ANOVA was used to analyze 8ISO, 3NT, and LOOH. Paired samples t-tests were used to compare metabolic biomarkers. Data presented as mean±SEM. RESULTS: CT elicited decrease in total cholesterol (TC) (173.6±12.1 to 153.4±8.6 mg·dL-1, p=0.01), hemoglobin A1c (5.2±0.1 5.1±0.1 %, p=0.003), hemoglobin (15.5±0.4 to 14.3±0.3 g·dL- 1, p=0.003), and estimated plasma volume to (53.8±0.7 to 50.7±1.4 %, p=0.005) from day 1 to 11. No difference was observed in high- density lipoprotein cholesterol. A main effect for time was observed in 8ISO (p- 1). 3NT was significantly elevated from day 4 to day 8 (d4: 2.4±0.6, d8: 2.9±0.6 mg·mL-1). LOOH showed a significant increase across all days (d1: 2.2±0.4, d4: 2.8±0.5, d8: 3.4±0.5, d11: 4.0±0.6 mM). Fitness was significantly correlated with DTC (r=0.58, p=0.046) and D8ISO (r=0.60, p=0.050). CONCLUSION: These data suggest the exertion required of WLFF during CT results in positive alterations to the metabolic profile. The changes in oxidative stress markers may reflect rapid adaptation to the CT stressors. These data suggest that WLFF are able to adapt quickly to the physical stress of CT, where fitness may be a protector of metabolic perturbations.
Funded by the US Forest Service 16-CR-11138200-005
Development of EMT/TS Co-simulation Using PowerFactory and PSS/E
No full textAs the scale and complexity of power systems increase, simulating them in efficient and accurate ways continues tobe a challenge in power systems engineering. Electromagnetic Transient (EMT) and Transient Stability (TS) simulation are the two main dynamic power system simulation methods. To simulate large and complex power systems in sufficient detail without sacrificing execution time, one of the idea is to perform a co-simulation that couples EMT and TS simulator. Although several attempts have been made to couple both simulator, only on rare occasions do these hybrid EMT-TS simulators couple two or more industry-adopted simulation tools.The objective of this thesis is to to develop and study the benefits and limitations of the Electromagnetic Transient – Transient Stability co-simulation based on PowerFactory and PSS/E, both of which are among the most extensively used simulation tools in industry and academia alike. With regards to the objective, the EMT-TS co-simulation using PowerFactory and PSS/E has been developed. Then, several tests are performed to evaluate the function of each composing part of the EMT-TS co-simulation, and to test the integration between all its component. Next, The developed co-simulation is applied to study cases and the results are compared to a monolithic EMT simulation to evaluate its accuracy and execution time. Furthermore, the effect of TS and EMT time step to the accuracy and execution time of EMT-TS co-simulation have also been investigated.The study case results show that the developed EMT-TS co-simulation has not been beneficial yet in terms of accuracy and execution time. Although the active power result shows a similar tendency with the monolithic EMT result, the difference between both are visible. The difference between both are more prominent in the reactive power result. The total execution time of the developed co-simulation in the study cases are in the range of 23-24 minutes, significantly larger than the total execution time obtained from the monolithic EMT simulation which is around of 12s. Also, it is found that reducing the TS time step from 0.02 s to 0.01 s slightly increases the total simulation time from 23 to 26 minutes. However, it does not contribute a significant improvement on the accuracy of the developed EMT-TS co-simulation. The result obtained from reducing the EMT time step to is the same with the result obtained using EMT time step. Moreover, the reduction of the EMT time step significantly increases the total simulation time from 23 to 42 minutes. The developed co-simulation still has a lot of room for improvement and further developments in this topic might increase its performance.Electrical Engineering | Sustainable Energy Technolog
TS-MPC for autonomous vehicles Including a TS-MHE-UIO estimator
No full text© 2019 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.In this paper, a novel approach is presented to solve the trajectory tracking problem for autonomous vehicles. This approach is based on the use of a cascade control where the external loop solves the position control using a novel Takagi Sugeno-Model Predictive Control (TS-MPC) approach and the internal loop is in charge of the dynamic control of the vehicle using a Takagi Sugeno-Linear Quadratic Regulator technique designed via Linear Matrix Inequalities (TS-LMI-LQR). Both techniques use a TS representation of the kinematic and dynamic models of the vehicle. In addition, a novel Takagi-Sugeno estimator-Moving Horizon Estimator-Unknown Input Observer (TS-MHE-UIO) is presented. This method estimates the dynamic states of the vehicle optimally as well as the force of friction acting on the vehicle that is used to reduce the control efforts. The innovative contribution of the TS-MPC and TS-MHE-UIO techniques is that using the TS model formulation of the vehicle allows us to solve the nonlinear problem as if it were linear, reducing computation times by 10-20 times. To demonstrate the potential of the TS-MPC, we propose a comparison between three methods of solving the kinematic control problem: Using the nonlinear MPC formulation (NL-MPC) with compensated friction force, the TS-MPC approach with compensated friction force, and TS-MPC without compensated friction force.This work was supported by the Spanish Min-istry of Economy and Competitiveness (MINECO) and FEDER through theProjects SCAV (ref. DPI2017-88403-R) and HARCRICS (ref. DPI2014-58104-R). The corresponding author, Eugenio Alcalá, is supported under FI AGAURGrant (ref 2017 FI B00433).Peer ReviewedPostprint (author's final draft
PENERAPAN MODEL PEMBELAJARAN KOOPERATIF TIPE TWO STAY TWO STRAY (TS-TS) TERHADAP KEMAMPUAN KOMUNIKASI MATEMATIKA SISWA
No full textThe problem that the author encountered in class X SMK Palapa Bandar Lampung is the low ability of mathematical communication. The purpose of the study was to determine the difference in average mathematical communication skills of students who use the Two Stay Two Stray (TS-TS)cooperative learning model with TS-TS using the Conventional learning model in class X students even semester of SMK Palapa Bandar Lampung. This study used an experimental method, the population of the study was 123 students of SMK Palapa Bandar Lampung, samples taken using the Random Sampling Cluster technique, namely the X PM class as the Experiment class and the AP X Class as the Control class. Students\u27 mathematical communication skills are carried out with an essay test which amounts to 6 questions which are first tested for their validity and reliability. From the results of testing the hypothesis using the t-test obtained thit = 4.94 from the t distribution table at a significant level of 5% known tdaf = 1.67. So thit > tdaf so that it can be concluded that "There is an Effect of the Application of the Two Stay Two Stray Cooperative Learning Model (TS-TS) on the Mathematical Communication Ability of Class X Students in the even semester of SMK Palapa Bandar Lampun
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