1,721,261 research outputs found
Replication Data for: Pride: A Catalyst for Academic Excellence. Pride on Academic Performance and Student Engagement
No full textHere is the data from which our study draws conclusions
Los Banu Yuzayy. Una familia de juristas e intelectuales granadinos del siglo XIV. I: Abu l-Qasim Muhammad Ibn Yuzayy
Full text linkVersan estas páginas sobre una familia de intelectuales granadinos del s. XIV, los Banu Yuzayy, y muy especialmente, en esta ocasión, de la vida y la obra de Abu l- Qasim Muhammad (693-741/1294-1340), importante figura en el campo de la liteatura jurídica malikí y autor de algunos poemas del género ascético y filosófico-moral (hikma), que fue padre de tres personajes relevantes en el panorama cultural del reino nazarí de Granada en quienes se centrarán las sucesivas entregas de este artículo. Para ello se han vertido al español los artículos consagrados a Abu l-Qasim en diferentes fuentes que van desde el siglo XIV hasta el XVII, entre ellas, la Ihata, el Natir al-yuman o el Nafh al-tib.These pages deal with a learned family from Granada of the fourteenth century, the Banu Yuzayy and particularly about the life and works of Abu l-Qasim Muhammad, an outstanding figure in the maliki juridical literature. He also composed a few poems on the ascetic and philosophic-moral genre (hikma). This fakih was the father of three important personages on the cultural environment in the Nasri Kingdom of Granada. They will be the subject of a number of consecutive works in the future. With this purpose have been translated to Spanish the biographies consecrated to this figure in different sources from the XIVth century to the XVIIth as the Ihata, the Natir al-yuman or the Nafh al-tib
A new species of Stenodynerus (Hymenoptera, Vespidae, Eumeninae) from Pakistan
No full textQasim, Muhammad, Carpenter, James M., Rafi, Muhammad Ather, Khan, Muhammad Rafique, Khan, Muhammad Rahim (2018): A new species of Stenodynerus (Hymenoptera, Vespidae, Eumeninae) from Pakistan. Zootaxa 4370 (3): 271-274, DOI: 10.11646/zootaxa.4370.3.
FIGURE 3 in A new species of Stenodynerus (Hymenoptera, Vespidae, Eumeninae) from Pakistan
No full textFIGURE 3. Stenodynerus punjabensis, head (frontal view)Published as part of <i>Qasim, Muhammad, Carpenter, James M., Rafi, Muhammad Ather, Khan, Muhammad Rafique & Khan, Muhammad Rahim, 2018, A new species of Stenodynerus (Hymenoptera, Vespidae, Eumeninae) from Pakistan, pp. 271-274 in Zootaxa 4370 (3)</i> on page 273, DOI: 10.11646/zootaxa.4370.3.5, <a href="http://zenodo.org/record/1145539">http://zenodo.org/record/1145539</a>
FIGURE 1 in A new species of Stenodynerus (Hymenoptera, Vespidae, Eumeninae) from Pakistan
No full textFIGURE 1. Stenodynerus punjabensis, habitus (dorsal view).Published as part of <i>Qasim, Muhammad, Carpenter, James M., Rafi, Muhammad Ather, Khan, Muhammad Rafique & Khan, Muhammad Rahim, 2018, A new species of Stenodynerus (Hymenoptera, Vespidae, Eumeninae) from Pakistan, pp. 271-274 in Zootaxa 4370 (3)</i> on page 272, DOI: 10.11646/zootaxa.4370.3.5, <a href="http://zenodo.org/record/1145539">http://zenodo.org/record/1145539</a>
FIGURE 2 in A new species of Stenodynerus (Hymenoptera, Vespidae, Eumeninae) from Pakistan
No full textFIGURE 2. Stenodynerus punjabensis, habitus (lateral view).Published as part of <i>Qasim, Muhammad, Carpenter, James M., Rafi, Muhammad Ather, Khan, Muhammad Rafique & Khan, Muhammad Rahim, 2018, A new species of Stenodynerus (Hymenoptera, Vespidae, Eumeninae) from Pakistan, pp. 271-274 in Zootaxa 4370 (3)</i> on page 272, DOI: 10.11646/zootaxa.4370.3.5, <a href="http://zenodo.org/record/1145539">http://zenodo.org/record/1145539</a>
Identification of the Novel Interacting Partners of the Mammalian Target of Rapamycin Complex 1 in Human CCRF-CEM and HEK293 Cells
No full textThe present study was undertaken to identify proteins that interact with the mammalian target of rapamycin complex 1 (mTORC1) to enable it to carry out its crucial cell signaling functions. Endogenous and myc-tag mTORC1 was purified, in-gel tryptic digested and then identified by nano-LC ESI Q-TOF MS/MS analysis. A total of nine novel interacting proteins were identified in both endogenous and myc-tag mTORC1 purifications. These new mTORC1 interacting partners include heterogeneous nuclear ribonucleoproteins A2/B1, enhancer of mRNA decapping protein 4, 60S acidic ribosomal protein, P0, nucleolin, dynamin 2, glyceraldehyde 3 phosphate dehydrogenase, 2-oxoglutarate dehydrogenase, glycosyl transferase 25 family member 1 and prohibitin 2. Furthermore hnRNP A2/B1 and dynamin 2 interaction with mTORC1 was confirmed on immunoblotting. The present study has for the first time identified novel interacting partners of mTORC1 in human T lymphoblasts (CCRF-CEM) and human embryonic kidney (HEK293) cells. These new interacting proteins may offer new targets for therapeutic interventions in human diseases caused by perturbed mTORC1 signaling
Differential proteome analysis of human embryonic kidney cell line (HEK-293) following mycophenolic acid treatment
No full textAbstract
Background
Mycophenolic acid (MPA) is widely used as a post transplantation medicine to prevent acute organ rejection. In the present study we used proteomics approach to identify proteome alterations in human embryonic kidney cells (HEK-293) after treatment with therapeutic dose of MPA. Following 72 hours MPA treatment, total protein lysates were prepared, resolved by two dimensional gel electrophoresis and differentially expressed proteins were identified by QTOF-MS/MS analysis. Expressional regulations of selected proteins were further validated by real time PCR and Western blotting.
Results
The proliferation assay demonstrated that therapeutic MPA concentration causes a dose dependent inhibition of HEK-293 cell proliferation. A significant apoptosis was observed after MPA treatment, as revealed by caspase 3 activity. Proteome analysis showed a total of 12 protein spots exhibiting differential expression after incubation with MPA, of which 7 proteins (complement component 1 Q subcomponent-binding protein, electron transfer flavoprotein subunit beta, cytochrome b-c1 complex subunit, peroxiredoxin 1, thioredoxin domain-containing protein 12, myosin regulatory light chain 2, and profilin 1) showed significant increase in their expression. The expression of 5 proteins (protein SET, stathmin, 40S ribosomal protein S12, histone H2B type 1 A, and histone H2B type 1-C/E/F/G/I) were down-regulated. MPA mainly altered the proteins associated with the cytoskeleton (26%), chromatin structure/dynamics (17%) and energy production/conversion (17%). Both real time PCR and Western blotting confirmed the regulation of myosin regulatory light chain 2 and peroxiredoxin 1 by MPA treatment. Furthermore, HT-29 cells treated with MPA and total kidney cell lysate from MMF treated rats showed similar increased expression of myosin regulatory light chain 2.
Conclusion
The emerging use of MPA in diverse pathophysiological conditions demands in-depth studies to understand molecular basis of its therapeutic response. The present study identifies the myosin regulatory light chain 2 and peroxiredoxin 1 along with 10 other proteins showing significant regulation by MPA. Further characterization of these proteins may help to understand the diverse cellular effects of MPA in addition to its immunosuppressive activity.
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Crosstalk between Edc4 and Mammalian Target of Rapamycin Complex 1 (mTORC1) Signaling in mRNA Decapping
No full textThe mammalian target of rapamycin complex 1 (mTORC1) is involved in the cellular transcription and translation processes. The undertaken study characterized the enhancer of mRNA decapping protein 4 (Edc4) as mTORC1 interacting protein. Human T lymphoblast (CCRF-CEM) cells were used for mTORC1 purification. Co-immunoprecipitation coupled with immunoblotting analysis was used to confirm the interaction of Edc4 in mTORC1 specific purifications. Further assays were incorporated to conclude the role of mTORC1 in mRNA decapping via Edc4. Edc4 was identified as a new interacting protein with mTORC1 in both the endogenous and myc-tag raptor component mTORC1 specific purifications. Quantitative co-localization using confocal microscopy demonstrated that raptor component of mTORC1 coexists with Edc4 in processing (P) bodies, a site for mRNA degradation. Incubation of cells with rapamycin, a known inhibitor of mTOR kinase activity, increased the total Edc4 protein expression but at the same time decreased the Edc4 interaction with mTORC1. Moreover, rapamycin treatment resulted in a significant decrease in total serine phosphorylated Edc4 protein signal and the total 5\u27-capped mRNA. These findings provide the first evidence for the pivotal role of mTORC1 in Edc4 regulation. Further in-depth studies are required to get a complete understanding of molecular crosstalk between mTORC1 signaling and mRNA decapping pathway
Damage accumulation location under cyclic loading in the lumbar disc shifts from inner annulus lamellae to peripheral annulus with increasing disc degeneration
No full textIt is difficult to study the breakdown of lumbar disc tissue over several years of exposure to bending and lifting by experimental methods. In our earlier published study we have shown how a finite element model of a healthy lumbar motion segment was used to predict the damage accumulation location and number of cyclic to failure under different loading conditions. The aim of the current study was to extend the continuum damage mechanics formulation to the degenerated discs and investigate the initiation and progression of mechanical damage. Healthy disc model was modified to represent degenerative discs (Thompson grade III and IV) by incorporating both geometrical and biochemical changes due to degeneration. Analyses predicted decrease in the number of cycles to failure with increasing severity of disc degeneration. The study showed that the damage initiated at the posterior inner annulus adjacent to the endplates and propagated outwards towards its periphery in healthy and grade III degenerated discs. The damage accumulated preferentially in the posterior region of the annulus. However in grade IV degenerated disc damage initiated at the posterior outer periphery of the annulus and propagated circumferentially. The finite element model predictions were consistent with the infrequent occurrence of rim lesions at early age but a much higher incidence in severely degenerated discs. </p
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