18 research outputs found
A benzimidazole-based chemodosimeter for the fluorometric detection of Zn and Cu via 1,5 proton shifts and C–N bond cleavage
No full textHere, we report the design and synthesis of the fluorescent probe APBHN, which was derived from 2-
(1H-benzo[d]imidazol-2-yl)benzenamine and is capable of detecting intracellular Zn and Cu ions in the
micromolar range. Single-crystal X-ray analysis revealed that the structure of the ligand comprises a fused
cyclic system with a pendent naphthol moiety. With the addition of Zn and Cu ions the inherent fluorescence
behaviour of the ligand APBHN is perturbed via a chemodosimetric change that involves a 1,5
proton shift followed by C–N bond cleavage. Upon detailed analysis, it was found that the ligand forms
1 : 1 and 1 : 2 (metal to ligand) complexes with the corresponding metal ions. The detection limits of Zn2+
and Cu2+ were 5.59 μM and 0.148 μM, respectively, with APBHN, which are lower than the WHO guidelines
(76 μM for Zn2+ and 31.5 μM for Cu2+) for drinking water. Moreover, APBHN could be used as a practical,
visible colorimetric test kit for both Zn2+ and Cu2+. APBHN can efficiently detect Zn2+ and Cu2+ in
liver carcinoma cells with insignificant cytotoxicit
Characterization of a fluorescent hydrogel synthesized using chitosan, polyvinyl alcohol and 9-anthraldehyde for the selective detection and discrimination of trace Fe3+ and Fe2+ in water for live-cell imaging
No full textIdentification of over expressed proteins in oral submucous fibrosis by proteomic analysis
No full textEarly detection and identification of oral pre-malignancy or malignancy help in management of the disease and improve survival rates. Oral submucous fibrosis (OSMF)
is amajor threat to public health worldwide and especially in SoutheastAsian countries.Identification of biomarkers is a necessary step toward early diagnosis and treatment. In
this study, differentially expressed proteins between oral submucous fibrotic tissue and normal control tissues were recorded by proteomic analysis using two dimensional
electrophoresis (2DE) andMALDI TOF mass spectrometry. By proteomic analysis, 15 proteins were found to be upregulated and 10 proteins downregulated in the OSMF
tissues than the control tissues; among these identified proteins, Hsp-70 1B,Calreticulin, and Lumican variant exhibited higher expression in OSMF tissues compared to the
control tissues. Immunohistochemical analysis also showed elevated expression of these in OSMF tissues. Further validation was done by real time quantitative RT-PCR
analysis; gene expression of Hsp-70 1B, Calreticulin, and Lumican variant were significantly increased (6.2-, 3.3-, 2.8- fold, respectively), whereas Enolase 1 was
decreased by 0.5 fold in the OSMF tissues, consistent with proteomic results. The expression of proteins indicates that various cellular signaling pathways must be
involved in the processes of fibrosis and suggests that expressed protein molecules play an important role in the pathogenesis of OSMF. These identified proteins may be
potentially used in future studies of OSMF enabling to determine diagnostic marker or therapeutic targets of this precancerous condition of oral cavit
Expedient Synthesis of Phenanthro-Imidazo-Pyridine Fused Heteropolynuclear Framework via CDC coupling: A New Class of Luminophores
No full textAll the reagents and solvents used in this study, were purchased from Sigma Aldrich, Thermo Fischer Scientific and TCI chemicals respectively. Open capillary was used to measure all the melting points. IR spectrum of the solid sample was recorded in the range 500 to 3500 cm-1
in an FT-IR spectrometer in KBr cell. Bruker 600 MHz spectrometer was used to record all 1H and 13C
NMR spectra. EI mass spectral analysis was done using JEOL The Mstation JMS-700 instrument. All the UV & Fluorescence data were collected using Jasco & Cary Eclipse
spectrophotometer respectively. Bruker Kappa Apex II X-raycrystallography machine was used to solve the crystal structure. Singlet (s), doublet (d), triplet (t) & multiplet (m) were designated as 1H NMR multiplicity patterns. Silica gel (60-120 mesh) and (100-200 mesh) were used for column chromatographic separations
Pyrrolo[3,4‑c]pyridine-Based Fluorescent Chemosensor for Fe3+/Fe2+ Sensitivity and Their Application in Living HepG2 Cells
No full textRole of matrix metalloproteinase-9 polymorphisms in basement membrane degradation and pathogenesis of oral submucous fibrosis
No full textCellular Detection of Hydrazine as Isoniazid Metabolite by a New Turn‐On Fluorescent Probe: Synthesis, Live Cell Imaging and In Vitro Toxicity Studies
No full textSynthetic Modulation of a Chemosensor Affords Target Metal Ion Switch from Zn<sup>2+</sup>to Al<sup>3+</sup>
No full textSolvent‐Dependent Nanostructures Based on Active π‐Aggregation Induced Emission Enhancement of New Carbazole Derivatives of Triphenylacrylonitrile
No full textSynthesis and DNA-Binding Studies of A New Cyclic Dimeric Symmetrical Pseudo-Turn Mimetic
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