1,356,399 research outputs found
Coletânea Consolida Prodan: Programa de Pós-Graduação Profissional em Dança: livro 2
No full textO livro registra os avanços decorrentes da implementação do Programa de Pós-Graduação
Profissional em Dança (Prodan), primeiro Mestrado Profissional em Dança do país, na
Universidade Federal da Bahia em 2019. Conforme os processos e resultados alcançados
pelos mestrandos e egressos,bBusca compreender a natureza das formas de conhecimentos
produzidos, investigando paradigmas e propósitos. Este ebook é o segundo entre quatro em
uma coletânea que marca a conclusão do primeiro quinquênio do Prodan, com propostas
didáticas-metodológicas, parte dos materiais didáticos apresentados como produções técnico-
tecnológicas, no TCC do Mestrado Profissional em Dança da UFBA de pessoas egressas da
linha do programa Processos Pedagógicos, Mediações e Gestão Educacional em Danç
Interaction of prodan with tubulin
No full textThe compound 6-propionyl-2-(N,N-dimethyl)-aminonaphthalene (Prodan), an efficient fluorescent probe for proteins, is shown to bind to tubulin. Detailed experiments on fluorescence enhancement, anisotropy and energy transfer were carried out to unravel the nature of Prodan-tubulin interaction and the Prodan-binding site on tubulin. It was found that Prodan binds to tubulin at a rigid site, with a stoichiometry of 1:1 and a dissociation constant of 20 μM. Competition experiments using 1,8-[3H]anilinonaphthalenesulfonate ([3H]ANS) indicated that the three aminonaphthalenes, ANS, bis(1,8-anilinonaphthalenesulfonate) and Prodan probably bind to a common region on the tubulin molecule
Profiling of dynamics in protein–lipid–water systems: a time-resolved fluorescence study of a model membrane protein with the label BADAN at specific membrane depths
No full textProfiles of lipid-water bilayer dynamics were determined from picosecond time-resolved fluorescence spectra of membrane-embedded BADAN-labeled M13 coat protein. For this purpose, the protein was labeled at seven key positions. This places the label at well-defined locations from the water phase to the center of the hydrophobic acyl chain region of a phospholipid model membrane, providing us with a nanoscale ruler to map membranes. Analysis of the time-resolved fluorescence spectroscopic data provides the characteristic time constant for the twisting motion of the BADAN label, which is sensitive to the local flexibility of the protein-lipid environment. In addition, we obtain information about the mobility of water molecules at the membrane-water interface. The results provide an unprecedented nanoscale profiling of the dynamics and distribution of water in membrane systems. This information gives clear evidence that the actual barrier of membranes for ions and aqueous solvents is located at the region of carbonyl groups of the acyl chains
A New Interpretation of the Absorption and the Dual Fluorescence of Prodan in Solution
No full textProdan is a fluorescent probe used to monitor biological systems such as lipid membrane,proteins and DNA. Remarkable interest is associated to the interpretation of its fluorescent spectrum.In this paper the sequential hybrid Quantum Mechanics/Molecular Mechanics (S-QM/MM) method was used to establish that the fluorescent emission occurs from two different excited states, resulting in a broad asymmetric emission spectrum with two transitions. The absorption spectra in several solvents were measured and calculated using different theoretical models and they all agree that the first observed band is composed of three electronic excitation very close energetically to each other
Surface properties of cholesterol-containing membranes detected by Prodan fluorescence
No full textAbstractThe fluorescent membrane probe 6-propionyl-2-dimethylaminonaphthalene (Prodan) displays a high sensitivity to the polarity and packing properties of lipid membrane. Contrary to 6-lauroyl-2-dimethylaminonaphthalene (Laurdan), Prodan can also monitor the properties of the membrane surface, i.e., the polar-head pretransition. In bilayers composed of coexisting gel and liquid–crystalline phases, Prodan shows a preferential partitioning in the latter, so that the detected membrane properties mainly belong to fluid domains. In the presence of cholesterol, the packing properties of the gel phase phospholipids are modified in such a way that Prodan can penetrate and label the membrane. Although Prodan labeling of the gel phase is a function of cholesterol concentration, 3 mol percent cholesterol is sufficient for a 60% Prodan labeling with respect to the maximum labeling reached at 15 mol percent cholesterol. We present steady-state and dynamical fluorescence measurements of Prodan in bilayers in the presence of cholesterol. Our results fit the liquid-ordered/liquid-disordered phase model for cholesterol-containing membranes and show that the presence of cholesterol, in addition to modification to the phase state of the hydrophobic portion of the bilayer, strongly affects the packing and the polarity of the membrane hydrophobic–hydrophilic interface
PRODAN differentially influences its local environment
No full textEnvironmentally-sensitive membrane dyes have been extensively used to study the different liquid phases, (liquid-ordered (Lo) and liquid-disordered (Ld)) of the heterogenous cellular membrane. However, it is not yet well understood how these dyes affect membrane properties upon and post insertion. Using a combination of molecular dynamics (MD) simulations and fluorescence microscopy, we study the effect of PRODAN insertion upon its local environment. We firstly present the results of the MD simulations of PRODAN interacting with lipid bilayers of various compositions, specifically the resultant hydration and lipid order of the system. Experimentally, the lipid order of Lo and Ld vesicles containing various concentrations of PRODAN are inferred from their Generalised Polarisation (GP) values, calculated using their fluorescence spectra. We then apply the methodology to a more complex biological system, the HeLa cell line. For both systems, the presence of PRODAN influences its local environment differently between the Lo and Ld phases. In the simulated systems, the presence of PRODAN lowers the lipid order in the Ld phase and increases the order in the Lo phase, whilst experimental data demonstrates that even a small increase in PRODAN concentration significantly lowers the order of both phases. We suggest this discrepancy may be ascribed to the differing localisations of the dye molecules within the bilayer, and their effect on the hydration of adjacent lipids
Does PRODAN Possess a Planar or Twisted Charge-Transfer Excited State? Photophysical Properties of Two PRODAN Derivatives
No full textThe synthesis and photochemical properties of 1-(4-methyl-1,2,3,4-tetrahydrobenzo[f]quinolin-8-yl)propan-1-one (7) and 2,2-dimethyl-1-(4-methyl-1,2,3,4-tetrahydrobenzo[f]quinolin-8-yl)propan-1-one (8) are reported.
These compounds are models for PRODAN, 6-propionyl-2-(dimethylamino)naphthalene, where the dialkylamino group is forced to remain coplanar with the naphthalene ring. The Stokes shifts of 7 and 8 in various
solvents are compared with those of PRODAN. The absorption and emission transitions are calculated by the
AM1 semiempirical method employing a conductor-like screening model for solvent effects. The experimental
and calculated solvatochroism of both model compounds are of the same magnitude as PRODAN, suggesting
that all fluoresce from a planar ICT state
Development of a cold end filament feeder for a hanging 3D printer
No full textHitrost tiskanja 3D tiskalnikov, ki uporabljajo tehnologijo ekstrudiranja polimerne niti (FFF) je v veliki meri odvisna od hitrosti podajalnika polimerne niti. V zaključni nalogi obravnavamo razvoj podajalnika polimerne niti za viseč 3D tiskalnik, ki bo zmogel večjo podajalno hitrost kot obstoječi podajalniki. Predstavljen je postopek od zastavljanja konstrukcijskih zahtev, preko preučevanja delnih funkcij podajalnika, do ocenjevanja prototipov. Na osnovi ocen delnih rešitev smo prišli do končnega prototipa podajalnika.The speed of 3D printers using fused filament fabrication (FFF) technology depends mostly on the speed of the extruder. In this document we discuss the development of a cold end of extruder for a large-scale 3D printer. A presented process starts from the setting of construction requirements, go through the study of the partial functions of the cold end and finish with the evaluation of prototypes. Based on the estimates of partial solutions we create final prototype of cold end of extruder
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