122,486 research outputs found
Procopio de Camargo (Candido) Aspectos sociologicos del espiritismo en SaoPaulo
Bastide Roger. Procopio de Camargo (Candido) Aspectos sociologicos del espiritismo en SaoPaulo. In: Archives de sociologie des religions, n°13, 1962. pp. 187-188
Ferreira de Camargo (Candido Procopio) Igreja e Desenvolvimento
Bastide Roger. Ferreira de Camargo (Candido Procopio) Igreja e Desenvolvimento. In: Archives de sociologie des religions, n°33, 1972. pp. 236-237
Ferreira de Camargo (Candido Procopio) Igreja e Desenvolvimento
Bastide Roger. Ferreira de Camargo (Candido Procopio) Igreja e Desenvolvimento. In: Archives de sociologie des religions, n°33, 1972. pp. 236-237
Exploring the World of “Touch DNA” and “Touch Microbiome”: One-Shot DNA Collection and Extraction
Microorganisms are present on both the interior and exterior surfaces of the human body, outnumbering human cells at a 3:1 ratio.1 Like human
DNA shed from skin cells, microbes are also transferred to the surrounding environment.2,3 Due to the robust structure of bacterial DNA, it withstands degradation
more effectively than human DNA. Given the increasing role of microbiome analyses in forensics, and the presence of enough microbial DNA in a fingerprint to
generate a reliable profile, this presentation examines the transfer and recovery of touch DNA and microbiomes using 4N6FLOQSwabs.2
At a crime scene, the simultaneous collection of fingermark ridge patterns and related evidence can be challenging. When a fingermark is compromised, investigators
are often advised to prioritize DNA recovery over dactyloscopy. Fingermarks frequently contain low quantities of DNA, sometimes below the 0.002ng/μL threshold
for obtaining a meaningful Short Tandem Repeat (STR) profile.4 Despite this, forensic DNA analyses can still yield high-quality profiles.5-7 Additionally, a person’s
microbial signature can reveal more about their lifestyle and health than human DNA alone. By analyzing both nuclear DNA (nDNA) and microbial DNA from the
same swab, investigators can gain further insights, using established forensic genetics methods while maintaining the accuracy of human identification profiles.
A total of 40 participants provided fingermarks of their Dominant Hand’s Index (DHI) on a standardized and sterilized glass surface. Prior to deposition, participants
washed their hands and allowed natural “recharge” for one hour. Fingermarks were deposited using a cutout mold, applying 500g pressure for 60s. DNA was
collected by swabbing the entire fingermark with dried flocked 4N6FLOQSwabs. NDNA extraction was done using the Qiagen QIAamp DNA Blood and Tissue
kit.8 DNA quantitation was conducted using the Human Quantifiler kit on a 7500 Real-Time PCR System. Human DNA was amplified by AmpFLSTR Identifiler
Plus PCR Amplification Kit on a 9700 GeneAmp PCR System. The amplified samples were processed by capillary electrophoresis and STRs analyzed by
GeneMapper ID 3.2 software. Using the same extracts, the V4 region of the 16s rRNA gene was amplified and sequenced using the Illumina MiSeq System. FASTq
files were analyzed in QIIME2 (ver. 2024.5.0), were quality filtered using DADA2, and trimmed accordingly. Taxonomic assignments were made using silva-138-
99. Data were further analyzed in R (version 4.2.3) with the “Phyloseq” packages.
Results showed that the co-extraction of touch DNA and touch microbiome is possible, and the use of standardized conditions for the deposition and collection of
the fingermark significantly improves the success of the analysis when compared with previous studies conducted in less ideal and optimized conditions.3 The
microbial profiles obtained from the various fingers from the same donor are distinct one from another one but still more similar than between individuals in terms
of bacterial composition, bacterial relative abundance, alpha and beta diversity. Specific taxa (ASVs) unique to specific donors were found in the deposited finger
marks after excluding the environmental signature found on the glass slide, highlighting the potential use of touch microbiome as an additional tool to identify the
donor of the trace found on touched objects on the crime scene.
On the basis of our preliminary results, it is possible to affirm that the obtention of human DNA and microbial DNA with standardized approaches may help forensic
analysts in performing a more appropriate personal identification and simultaneously obtain information about habits of a suspect
Bitensorial formulation of the singularity method for Stokes flows
This paper develops the bitensorial formulation of the system of singularities associated with unbounded and bounded Stokes flows. The motivation for this extension is that Stokesian singularities and hydrodynamic fundamental solutions are multi-point functions, and bitensor calculus provides either the proper geometrical setting, in order to avoid inconsistencies and misunderstandings on the role of the different tensorial indices, or a way for compactly deriving hydrodynamic properties. A first relevant result is to provide a clear definition of the singularities (both bounded and unbounded) in Stokes flow, specifying the associated differential equations and boundary conditions. Using this formalism for bounded flows, we show the existence of an integro-differential operator providing the whole system of hydrodynamic singularities by acting on the unbounded Green function (Stokeslet) at its pole and we derive its explicit representation in terms of moments. In the case of an immersed body in a unbounded fluid, we show that, the operator furnishing the disturbance field of a purely -th order ambient flow, is a generalized -th order Faxén operator, i.e., it yields the -th moment on the body if applied to a generic ambient flow, and that a generic disturbance field can be expressed by a summation of the generalized -th order Faxén operators. Furthermore, we find that the operator providing the disturbance of an ambient flow coincides with the reflection operator for the Stokes solutions in the same flow geometry. We apply this result to the paradigmatic case of fundamental singularities for the Stokes flow bounded by a plane. In this way, we obtain in an alternative and easy way the image system for the Sourcelet and the Rotlet (already derived in the literature) and for the Source Doublet and the Strainlet (presented here for the first time)
Hydrodynamic characterization of finite-sized particle transport in confined microfluidic systems, Brownian motion and stochastic modeling of particle transport at microscale
In this thesis, the peculiar effects of the hydrodynamic confinement on the dynamic of a colloid in the Stokes regime have been addressed theoretically. Practical expressions, useful to investigate the transport of particles in complex geometries, have been provided for force, torque and higher order moments on the particle and for the disturbed velocity field of the fluid.
To begin with, a new formulation of the Stokesian singularity method is developed by introducing a bitensorial distributional formalism.
This formalism overcomes the ambiguities of the classical hydrodynamic formulation of the singularity method that limits its application in confined problems.
The formalism proposed permits naturally to distinguish between pole and field points of tensorial singular fields and to clearly define each singularity from its associated Stokes problem.
As a consequence of this approach an explicit expression for the singularity operator is provided, giving the disturbance field due to a body once applied to an ambient flow of the fluid. The operator is expressed in terms of the volume moments and its expression is valid regardless of the boundary conditions applied to the surface of the body.
The dualism between the singularity operator giving the disturbance flow of a n-th order ambient flow and the n-th order Faxén operator has been investigated. It has been found that this dualism, referred to as the Hinch-Kim dualism, holds only if the boundary conditions satisfy a property that is referred to as the Boundary-Condition reciprocity (BC-reciprocity, for short).
If this property is fulfilled, the Faxén operators can be expressed in terms of (m,n)-th order geometrical moments of volume forces (defined in Chapter 3). In addition, it is shown that in these cases, the hydromechanics of the fluid-body system is completely determined by the entire system of the Faxén operators. Classical boundary conditions of hydrodynamic practice (involving slippage, fluid-fluid interfaces, porous materials, etc.) are investigated in light of this property.
It is found the analytical expression for the 0-th, 1-st and 2-nd Faxén operators for a sphere with Navier-slip boundary conditions.
These results are applied in order to express the hydrodynamics of particles in confined fluids in terms of quantities related to the geometry of the particle and the geometry of the confinement separately using the reflection method.
Specifically, closed-form results and practical expressions for the velocity field of the fluid and the functional form of force and torque acting on a particle are derived in terms of: (i) the Faxén operators of the body of the particle (given by its unbounded geometrical moments) and (ii) the multi-poles in the domain of the confinement.
The convergence of the reflection method is examined and it is found that the expressions obtained are also valid for distances between particle and walls of the confinement of the same magnitude order, failing only in the limit case of the lubrication range.
The reflection solutions obtained with the present theory, approximated to the order O(lb/ld)^5 ("lb" being the characteristic size of the body and "ld" the characteristic distance from the confinement boundaries) are compared with the exact solution of a sphere near a planar wall, and the expressions for forces and torques considering the more general situation of Navier-slip boundary conditions on the body are provided.
A general formulation of the fluctuation-dissipation relations in confined geometries, the paradoxes associated with no-slip boundary conditions close to a solid boundary, and the modal representation of the inertial kernels for complex fluids complete the present dissertation.
Specifically, the general setting of the overdamped approximation in confined geometries is provided, by explicitly expressing the thermal contributions associated with the rigid rototranslational motion of a body. In passing, the extension of fluctuation-dissipation results to non-equilibrium conditions, such as those arising in thermophoretic flows in the presence of a steady temperature profile is developed.
The influence of boundary conditions on the fluctuational form of the force acting on a rigid particle near a solid wall is addressed, showing that the classical Stokesian paradox of infinite touching time in the presence of no-slip boundary conditions can be resolved by considering the arbitrarily small slippage effects on both surfaces, leading to an integrable logarithmic singularity.
Finally, a preliminary extension of fluid-particle interactions either in a time-dependent Stokes regime or in the presence of complex (viscoelastic) flows is addressed, focusing on the modal representation of the dissipative and fluid inertial memory kernels, and on the fluctional form of the latter.
Specifically, it is shown that for a viscoelastic fluid, characterized by a finite and non-vanishing relaxation rate, the generalized Basset kernel is a regular function of time, also close to t=0, which is not the case of a Newtonian fluid for which the Basset kernel scales inversely with the square root of t
La voragine di “San Procopio”a Barletta: analisi preliminari, rilievi speleologici e geofisici per la mappatura della pericolosità geomorfologica
Tra la sera del 2 maggio e le prime ore del
3 maggio 2010, in località San Procopio
in agro di Barletta, si verificava uno
sprofondamento di importanti dimensioni.
La voragine, di forma all’incirca circolare,
di diametro pari a circa 32 m e superficie
netta di 800 m2 circa, interessava svariati alberi
d’ulivo, un traliccio Enel e interrompeva
la strada comunale Via Vecchia Minervino. Ai
margini dello sprofondamento, profondo dai
5 ai 12 m, si intravedevano strutture cunicolari
di chiara origine antropica riconducibili
a gallerie sotterranee per l’estrazione della
calcarenite quale materiale da costruzione
(localmente detta “tufo” calcareo), dall’interno
delle quali i dissesti si erano propagati
alla superficie, determinando la formazione
della voragine
A vitamin E analogue suppresses malignant mesothelioma in a preclinical model: a future drug against a fatal neoplastic disease?
No Full Tex
Surgical and orthodontic treatment of skeletal Class III featuring severe transversal and sagittal discrepancy
Aims: Anterior cross-bite is a difficult malocclusion to treat in adult patients, especially if compounded by skeletal discrepancy. The present study describes a dentoskeletal Class III case and aims to provide the clinician with rational guidelines for presurgical orthodontic preparation and postsurgical finishing. Patient and methods: In this case, a 20-year-old male patient, R.M, was treated for severe dental and skeletal Class III malocclusion on both the transversal and anteroposterior planes via combined orthodontics and surgery. Initially, the treatment involved surgically-assisted expansion of the upper jaw (total 1 month), followed by a fixed-orthodontics phase to decompensate for the malocclusion in preparation for movement of the osseous bases with the aim of achieving maximum coordination of the dental arches. After 19 months of orthodontic preparation, the patient underwent combined orthognathic surgery (upper and lower jaws). In the subsequent 4 months, orthodontic stabilization and finishing were performed, and debonding was carried out 24 months after the start of active treatment. Results: The combined orthodontic and surgical treatment adequately corrected the severe Class III over a period of 2 years, leading to a satisfactory occlusal, functional and aesthetic result. Conclusions: Thorough diagnosis and close communication between the orthodontist and maxillofacial surgeon, operating as an interdisciplinary team, ensures good outcomes, even in complex orthodontic and surgical cases. � 2012
A Multi-Language Comparison of Influences on Author Verification using Character N-Grams
We create a new multi-language corpus for author verification based on Wikipedia talkpages, and evaluate the influence that differences in topic and time have on character n-gram author profiles. Topic alignment between two texts is found to increase author verification precision, and an authors writing style is found to change over time, but not more significantly after 3 years than after 1 year.Information ArchitectureWISElectrical Engineering, Mathematics and Computer Scienc
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