328 research outputs found
<b>Supplemental Material—Evaluation of Remodeling of Visceral Arteries and Impact on Renal Function Post-endovascular Repair of Type B Aortic Dissection Vis-A-Vis Baseline Visceral Artery Morphology</b>
No full textSupplemental Material for Evaluation of Remodeling of Visceral Arteries and Impact on Renal Function Post-endovascular Repair of Type B Aortic Dissection Vis-A-Vis Baseline Visceral Artery Morphology by Amit Ajit Deshpande, Niraj Nirmal Pandey, Manish Shaw, Sanjeev Kumar, Priya Jagia, and Shiv Choudhary in Vascular and Endovascular Surgery.</p
1,2,4-Triazine derivatives as agents for the prevention of AGE-RAGE-mediated inflammatory cascade in THP-1 monocytes: An approach to prevent inflammation-induced late diabetic complications
Get PDFIntroduction: Monocytes mainly contribute to the development and progression of vascular inflammatory conditions via the M1 polarization. The elevated levels of advanced glycation end products (AGEs) in diabetic environment lead to severe inflammation, and the release of pro-inflammatory mediators. This shifts the balance towards the pro-inflammatory state of monocytes. Objective: The current study was aimed to determine the antiglycation activity of 1,2,4-triazine derivatives, and study of their molecular basis in regulating the AGEs-mediated inflammatory responses in THP-1 monocytes. Methods: Primarily, the antiglycation activity of a series of 1,2,4-triazine derivatives was evaluated against MGO-AGEs in vitro. The toxicity of antiglycation compounds was determined by a metabolic assay, using human hepatocyte (HepG2) and monocyte (THP-1) cell lines. DCFH-DA probe was used to evaluate the antioxidant potential of the compounds. Immunocytochemistry, Western blotting, and ELISA techniques were employed to determine the levels of pro-inflammatory markers (NF-kappa B, RAGE, COX-1, COX-2, and PGE(2)) in THP-1 monocytes under in-vitro hyperglycemic conditions. Results: Results indicate that the triazine derivatives 22, and 23 were the most potent antiglycation agents among the entire series, while non-toxic to HepG2, and THP-1 cells. Both compounds inhibited the AGEs-induced upstream and downstream signaling of NADPH oxidase and inflammatory mediators p38 and NF-kappa beta, respectively, in THP-1 monocytes. They also inhibited the induction of COX-2 and its product PGE2 by suppressing AGE-RAGE interactions. Moreover, compounds 22, and 23 reversed the AGEs-mediated suppression of COX-1 in THP-1 monocytes. Conclusion: In conclusion, 1,2,4-triazine derivatives 22, and 23 have the potential to suppress inflammatory responses under the diabetic environment through AGE-RAGE-NF-kappa beta/p38 nexus in THP-1 monocytes. These findings identify triazines 22, and 23 as compelling candidates for drug development, potentially beneficial for the diabetic patients with an elevated risk of vascular complications, such as atherosclerosis
Toxicological profile for hexachlorobutadiene
Get PDFATSDR/TP-93/08Chemical manager(s)/author(s): Gangadhar Choudhary, Joyce M. Donohue, Yvonne N. Hales.Prepared by Life Systems, Inc., under subcontract to Clement International Corporation;prepared for U.S. Department of Health and Human Services, Public Health Service, Agency for Toxic Substances and Disease Registry under contract no. 205-88-0608.Includes bibliographical references (p. 105-128).205-88-060
Author response: Multiple short windows of calcium-dependent protein kinase 4 activity coordinate distinct cell cycle events during Plasmodium gametogenesis
No full textMalaria transmission relies on the production of gametes following ingestion by a mosquito. Here, we show that Ca2+-dependent protein kinase 4 controls three processes essential to progress from a single haploid microgametocyte to the release of eight flagellated microgametes in Plasmodium berghei. A myristoylated isoform is activated by Ca2+ to initiate a first genome replication within twenty seconds of activation. This role is mediated by a protein of the SAPS-domain family involved in S-phase entry. At the same time, CDPK4 is required for the assembly of the subsequent mitotic spindle and to phosphorylate a microtubule-associated protein important for mitotic spindle formation. Finally, a non-myristoylated isoform is essential to complete cytokinesis by activating motility of the male flagellum. This role has been linked to phosphorylation of an uncharacterised flagellar protein. Altogether, this study reveals how a kinase integrates and transduces multiple signals to control key cell-cycle transitions during Plasmodium gametogenesis.</jats:p
Microbial transformation of nandrolone with Cunninghamella echinulata and Cunninghamella blakesleeana and evaluation of leishmaniacidal activity of transformed products
No full textTherapeutic potential of nandrolone and its derivatives against leishmaniasis has been studied. A number of derivatives of nandrolone (1) were synthesized through biotransformation. Microbial transformation of nandrolone (1) with Cunninghamella echinulata and Cunninghamella blakesleeana yielded three new metabolites, 10β,12β,17β-trihydroxy-19-nor-4-androsten-3-one (2), 10β,16α,17β-trihydroxy-19-nor-4-androsten-3-one (3), and 6β,10β,17β-trihydroxy-19-nor-4-androsten-3-one (4), along with four known metabolites, 10β,17β-dihydroxy-19-nor-4-androsten-3-one (5), 6β,17β-dihydroxy-19-nor-4-androsten-3-one (6) 10β-hydroxy-19-nor-4-androsten-3,17-dione (7) and 16β,17β- dihydroxy-19-nor-4-androsten-3-one (8). Compounds 1-8 were evaluated for their anti-leishmanial activity. Compounds 1 and 8 showed a significant activity in vitro against Leishmania major. The leishmanicidal potential of compounds 1-8 (IC50 = 32.0 ± 0.5, andgt;100, 77.39 ± 5.52, 70.90 ± 1.16, 54.94 ± 1.01, 80.23 ± 3.39, 61.12 ± 1.39 and 29.55 ± 1.14 μM, respectively) can form the basis for the development of effective therapies against the protozoal tropical disease leishmaniasis. © 2014 Elsevier Inc. All rights reserved.Al-Aboudi A, 2009, STEROIDS, V74, P483, DOI 10.1016-j.steroids.2009.01.002; Alarcon J, 2007, J MOL CATAL B-ENZYM, V48, P23, DOI 10.1016-j.molcatb.2007.06.001; Antinarelli Luciana Mr, 2012, Org Med Chem Lett, V2, P16, DOI 10.1186-2191-2858-2-16; Borges KB, 2008, J PHARMACEUT BIOMED, V46, P945, DOI 10.1016-j.jpba.2007.05.018; Borges KB, 2009, TETRAHEDRON-ASYMMETR, V20, P385, DOI 10.1016-j.tetasy.2009.02.009; BORIS A, 1967, STEROIDS, V9, P299, DOI 10.1016-0039-128X(67)90114-6; CASPI E, 1989, J CHEM SOC CHEM COMM, P1699, DOI 10.1039-c39890001699; Choudhary MI, 2010, STEROIDS, V75, P956, DOI 10.1016-j.steroids.2010.05.017; Choudhary MI, 2008, NAT PROD RES, V22, P1289, DOI 10.1080-14786410500462660; Choudhary MI, 2005, CHEM BIODIVERS, V2, P392, DOI 10.1002-cbdv.200590019; Choudhary MI, 2011, STEROIDS, V76, P1288, DOI 10.1016-j.steroids.2011.06.007; Choudhary MI, 2009, STEROIDS, V74, P1040, DOI 10.1016-j.steroids.2009.08.003; Choudhary MI, 2007, STEROIDS, V72, P923, DOI 10.1016-j.steroids.2007.08.002; Choudhary MI, 2012, J ENZYM INHIB MED CH, V27, P348, DOI 10.3109-14756366.2011.590804; de Flines J, 1963, RECL TRAV CHIM PAY B, V82, P129; de Flines J, 1963, RECL TRAV CHIM PAY B, V82, P121; de Flines J, 1963, RECL TRAV CHIM PAY B, V82, P149; Desjeux P, 2004, COMP IMMUNOL MICROB, V27, P305, DOI 10.1016-j.cimid.2004.03.004; Farooq A, 2002, Z NATURFORSCH C, V57, P303; Hazra S, 2013, EXP PARASITOL, V135, P407, DOI 10.1016-j.exppara.2013.07.021; Hernandez-Torres A, 2013, SCAND J INFECT DIS, V45, P567, DOI 10.3109-00365548.2012.752859; Huszcza E, 2003, J BASIC MICROB, V43, P113, DOI 10.1002-jobm.200390011; Kolet SP, 2013, STEROIDS, V78, P1152, DOI 10.1016-j.steroids.2013.08.004; Kouvelas D, 2008, INT J NEUROPSYCHOPH, V11, P925, DOI 10.1017-S1461145708008754; Oliveira-Silva FD, 2008, AM J TROP MED HYG, V78, P745; Parshikov IA, 2000, APPL ENVIRON MICROB, V66, P2664, DOI 10.1128-AEM.66.6.2664-2667.2000; Shao-rui C, 2010, CHEM RES CHINESE U, V26, P922; Yazdi MT, 2006, ARCH PHARM, V339, P473, DOI 10.1002-ardp.2005002350
Estimation of Distribution of Income in Pakistan, Using Micro Data
Get PDFIncome distribution entered the post war discussion of economic development fairly late. Until the 1960s much of the focus was on industrialisation and the need for capital accumulation. Pakistan was no exception as in the early 60s economic expansion became the main target and means to political identity. Rapid population growth associated with steep decline in mortality demanded acceleration of production to keep pace. Overall aggregate expansion was much faster than before but without benefit for the poor. In that context emerged a new professional interest in income distribution. Haq’s (1964) study was one of the oldest studies conducted to measure inequality in personal income distribution in the high income brackets in the urban areas of Pakistan. The main objective of the author was to present the income distribution pattern in terms of the relative shares of different income groups as well as in terms of Pareto coefficients and concentration ratio during the period 1948-49 to 1957-58 for which published tax data was available. While recognising the limitations of the data used, the author went on to calculate various measures of income inequality including Pareto coefficient and Lorenz curve. The author also made comparison of Pakistan’s income distribution with U.S.A. and U.K.
A case of primary amenorrhea with swyer syndrome
No full textSwyer syndrome with complete gonadal dysgenesis is associated with an absence of testicular differentiation in a phenotypic female with a 46, XY karyotype. A 14-year-old unmarried girl was referred with complaints of primary amenorrhea and nondevelopment of breast. Her built was normal. Examination of her secondary sexual characteristics revealed no breast development, absent axillary hairs, and sparse pubic hairs. External genitalia was of female type. Karyotype showed genotype of 46, XY. Magnetic resonance imaging revealed hypoplastic uterus with absent fallopian tubes and ovaries. A diagnosis of Swyer syndrome was made. Laparoscopy showed infantile uterus, normal fallopian tubes, and streak gonads. Laparoscopic removal of streak gonads was done as there is a risk of gonadoblastoma in such cases. The patient was started on hormonal replacement therapy. Swyer syndrome results mainly due to mutation in certain genes such as SRY gene, which leads to failure of development of testis
Machine learning predicted magnetic entropy change using chemical descriptors across a large compositional landscape
Get PDFMagnetocaloric refrigeration has drawn considerable attention in the last few decades as it can positively disrupt the current cooling technology. Most research efforts focus on developing new magnetic materials in the laboratory by trial and error. Here we report a materials dataset developed using past experimental work comprising several important magnetocaloric material classes such as La(Fe,Si/Al)13, heusler alloys, manganites, Gd5(Si,Ge)4 family, rare-earth and metallic glasses as well as Laves phase compounds with their reported magnetic entropy changes, -ΔSM(T,H). Notable linear and non-linear machine learning models are implemented to predict the -ΔSM(T,H) of materials. Our analyses indicate that the Random Forest model outperforms the others with R2 of 0.82. We then use this model to screen a large magnetic materials database with nearly 40,000 compounds to identify potential new magnetocaloric materials operating near room temperature. MnGa2Sb2, CrGa2Sb2, SbSCl0.1I0.9, Sm3Te4, LaRhSn, SbSI, Tl0.58Rb0.42Fe1.72Se2, Cs0.86Fe1.66Se2, La2.1MnGe2.2 are some of the newly predicted compounds that could yield large magnetocaloric cooling performance."This article is published as Ucar, Huseyin, Durga Paudyal, and Kamal Choudhary. "Machine learning predicted magnetic entropy change using chemical descriptors across a large compositional landscape." Computational Materials Science 209 (2022): 111414.
DOI: 10.1016/j.commatsci.2022.111414.
Copyright 2022 The Author(s).
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0).
Posted with permission.
DOE Contract Number(s): AC02-07CH11358
Predicting Instructor Performance using Naïve Bayes Classification Algorithm in Data Mining Technique
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