1,721,016 research outputs found
Primary cell culture and adenovirus-mediated gene transfer in isolated human ventricular cardiomyocytes
No full textReduced ryanodine receptor-mediated SR Ca2+-leak in isolated adult cardiomyocytes upon adenovirus-mediated overexpression of FKBP12.6
No full textGene expression of FK506-binding proteins (FKBPs) in the human heart. Identification of an alternatively spliced transcript of FKBP12.6
No full textFkbp9: An alternative splice variant of the FK506-binding protein FKBP12.6 expressed in the human heart.
No full textGene Expression of Antioxidative Enzymes in the Human Heart : Increased Expression of Catalase in the End-Stage Failing Heart
No full textFkbp9: An alternative splice variant of the FK506-binding protein FKBP12.6 expressed in the human heart.
No full textReduced ryanodine receptor-mediated SR Ca2+-leak in isolated adult cardiomyocytes upon adenovirus-mediated overexpression of FKBP12.6
No full textPrimary cell culture and adenovirus-mediated gene transfer in isolated human ventricular cardiomyocytes
No full textGene expression of FK506-binding proteins (FKBPs) in the human heart. Identification of an alternatively spliced transcript of FKBP12.6
No full textGene expression of antioxidative enzymes in the human heart - Increased expression of catalase in the end-stage failing heart
No full textBackground-An increase in oxidative stress is suggested to be intimately involved in the pathogenesis of heart failure. However, gene expression of enzymes that metabolize reactive oxygen metabolites has not been investigated in the human heart. Methods and Results-Myocardial tissue homogenates of the left ventricular wall from hearts in end-stage failure due to dilated (DCM) or ischemic (ICM) cardiomyopathy (n=12 each), as well as from nonfailing donor hearts (n=12), were analyzed for mRNA levels of manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD), glutathione peroxidase (GPX), and catalase by Northern blot analyses. Protein levels of MnSOD, CuZnSOD, and catalase were determined by Western blot or ELISA. MnSOD, CuZnSOD, and GPX mRNA levels were similar in all 3 groups. In contrast, catalase mRNA levels were found to be increased by 123+/-23% in DCM hearts and by 93+/-10% in ICM hearts (P<0.01 each) compared with control hearts. Likewise, catalase protein levels were found to be increased in failing hearts (DCM by 90+/-10%, ICM by 90+/-13%; P<0.05 each) compared with control hearts. In addition, the observed upregulation of catalase mRNA and protein in failing hearts was attended by an increased catalase enzyme activity (DCM by 124+/-16%, ICM by 117+/-15%; P<0.01 each), whereas MnSOD, CuZnSOD, and GPX enzyme activity levels were unchanged in failing compared with nonfailing myocardium. Conclusions-Increased oxidative stress in human end-stage heart failure may result in a specific upregulation of catalase gene expression as a compensatory mechanism, whereas SOD and GPX gene expression remain unaffected
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