222 research outputs found
In-Vitro Effects of Cytokines on Normal and Myeloid Leukaemic Haemopoiesis
No full textThe growth of chronic myeloid leukaemic (CML) and acute myeloid leukaemic (AML) cells in vitro and their response to various cytokines were studied and compared to normal peripheral blood and bone marrow cells. In normal mononuclear cells (MNC) the three cytokines [granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-3 (IL-3)] had no appreciable difference at stimulating (colony-forming unit granulocyte-macrophage) CFU-GM-formation. By contrast, G-CSF was a more potent stimulator of CFU-CML, than GM-CSF or IL-3. Interleukin-4 (IL-4) augmented G-CSF-induced colony formation of CML chronic phase cells. Transforming growth factor beta1 (TGFbeta1) reduced G-CSF-induced CFU-CML from CML chronic phase (p< 0.005) and blast transformation MNC, but had no effect on normal CFU-GM stimulated by G- CSF. The growth of CML mononuclear cells in vitro could not be correlated to the white cell count or percentage of CD34+ cells present. The mechanisms for the inhibitory effects of TGFbeta1 on CML cells were studied using in-situ hybridisation techniques to demonstrate the expression of the G-CSF and IL-4 receptors. The results suggest that this response is more likely to be due to a difference in signal transduction or protein synthesis rather in transcription
Unifying Matter, Energy and Consciousness
No full textThis is the presentation related to the conference paper at the 11th International Conference on Mathematical Modeling in the Physical Sciences on September 5-8, 2022.
The Conference link is: https://www.icmsquare.net
This video can be streamed via YouTube link: https://youtu.be/Pby1TfEluqE
For more information, please contact the author (Dr. Mahendra Samarawickrama) via email: [email protected] </p
Increased risk for myelodysplastic syndrome in individuals with glutathione transferase theta 1 gene (GSTT1) defect
No full text
Adoptive transfer of cytomegalovirus-specific CTL to stem cell transplant patients after selection by HLA–peptide tetramers
No full textStem cell transplantation is used widely in the management of a range of diseases of the hemopoietic system. Patients are immunosuppressed profoundly in the early posttransplant period, and reactivation of cytomegalovirus (CMV) remains a significant cause of morbidity and mortality. Adoptive transfer of donor-derived CMV-specific CD8+ T cell clones has been shown to reduce the rate of viral reactivation; however, the complexity of this approach severely limits its clinical application. We have purified CMV-specific CD8+ T cells from the blood of stem cell transplant donors using staining with HLA–peptide tetramers followed by selection with magnetic beads. CMV-specific CD8+ cells were infused directly into nine patients within 4 h of selection. Median cell dosage was 8.6 × 103/kg with a purity of 98% of all T cells. CMV-specific CD8+ T cells became detectable in all patients within 10 d of infusion, and TCR clonotype analysis showed persistence of infused cells in two patients studied. CMV viremia was reduced in every case and eight patients cleared the infection, including one patient who had a prolonged history of CMV infection that was refractory to antiviral therapy. This novel approach to adoptive transfer has considerable potential for antigen-specific T cell therapy
Ecological Niche Modeling and Local Knowledge Predict New Populations of \u3cem\u3eGymnocladus assamicus\u3c/em\u3e a Critically Endangered Tree Species
No full textGymnocladus assamicus is a critically endangered tree species endemic to northeastern India. Local inhabitants traditionally used this species for a variety of purposes. However, rapid population declines led to the species being considered extinct, until fieldwork in 2004 to 2007 identified 14 discrete populations of 1 to 7 trees each. To overcome constraints on field surveys imposed by the region’s remoteness and rugged terrain, we targeted areas of further field inventories by estimating the potential distribution of the species. Ecological niche modeling enabled us to identify 26 sites which the model predicted to be highly suitable for the species’ occurrence. We conducted rapid field surveys at 14 of the most accessible of these predicted sites. New populations were discovered at 5 of the 14 surveyed sites. In the remaining 12 less accessible sites, we interviewed residents from nearby villages and obtained indirect evidence of populations at 5 additional sites, which remain to be confirmed by direct field observations. This study demonstrates the utility of niche modeling as a tool for locating new populations of rare and endangered species. Our results will enhance ongoing efforts towards in situ conservation of this endangered species
Reduced pyridine nucleotide linked oxidase systems in Euglena gracilis var. bacillaris, 1973
No full textThis study was designed to investigate the presence of selected reduced pyridine nucleotide oxidase systems in a streptomycin bleached strain of Euglena gracilis var. bacillaris. The enzymes studied were NADH and NADPH diaphorases, NADH and NADPH cytochrome c reductases, NADH and NADPH oxidases and NADH lipoyl dehydrogenase. For experimental purposes the cells were grown axenically in a medium in which tryptone and sodium acetate were the sole source of carbon. Cell-free extracts prepared via centrifugation were used as the source of all enzyme assays. The activities of enzymes were measured in a Beckman DB-G recording spectrophotometer. In the presence of substrate and enzyme the activity of diaphorases were determined by measuring the reduction of dichlorophenol indophenol (DCPIP) at 600 nm and the cytochrome c reductase activity was measured by the reduction of cytochrome c (increase in optical density) at 550 nm. The activity of oxidases was determined by following the oxidation of reduced nicotinamide adenine dinucleotide (NADH) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) spectrophotometrically at 340 nm. In the presence of enzyme and DL-lipomide the determination of lipoyl dehydrogenase activity was based on the oxidation of NADH (decrease in optical density) at 340 nm. The data obtained revealed the presence of NADH and NADPH diaphorases, NADH and NADPH cytochrome c reductases, NADH and NADPH oxidases and NADH lipoyl dehydrogenase in cell-free extracts of E. gracilis var. bacillaris. The effects of pH, protein concentration, substrate concentration, storage at -5 C, and the effects of metabolic inhibitors were determined spectrophotometrically. Utilizing polyacrylamide gel disc electrophoresis, NADH and NADPH diaphorase were shown to be molecularly heterogeneous. Results obtained show that there are numerous pathways for the oxidation of reduced pyridine nucleotides
Beyond Questions and Answers: Prompting Reflections and Deepening Understandings of Mathematics Using Multiple-Entry Logs
No full textThere is little discussion in mathematics education about methods of effective tutoring; in particular, in so-called office hour or tutorial sessions. Nevertheless, these sessions are as much occasions for interactive instruction as classrooms are, and deserve theoretical and pedagogical attention. By initiating discussion in this area we invite others to contribute critically to these beginnings. Clearly, discussions in this area will inform and be informed by classroom interactions. For this reason, though we focus on office-hour or tutorial interactions, we do indicate how the pedagogical tool we describe informs classroom practice
Gauging the market and non-market forces in Indian aquaculture industry for a strategic position in fish vaccines market
No full textIndian aquaculture segment has become a growth engine for the agriculture sector and is postulated to be one of the
key sources of food-protein supporting the growing Indian population. Significant potential for new fish vaccine
business was demonstrated previously using market and sales potential assessments; however, in addition to the
overall market attractiveness, estimating the market and non-market forces in an industry is vital to ensure
profitability in a new business. Therefore, this research directed attention to gauging the forces using Porter’s five
market forces (2008) and Prasad’s (2011) non-market forces (national culture and history) framework together with
measuring the benefits of complementors. Each of the forces was measured and key drivers within each force were
identified. A base case revenue scenario was built on forecasts and the impact of the major forces was tied to the
income statement to determine sensitivity of the revenue forecasts. The results suggest that the buyer power and the
substitute power are the major competitive forces. The power of complementor products was also found significant
and an entrant could counter the predicted losses in profitability due to influences of these market and non-market
forces by providing product-product bundles thus reaping considerable improvements in net present value (NPV) and
internal rate of returns (IRR) on investments. The NPV from this analysis thus yielded a range between 7.5 million with an IRR ranging between 29.9% and 35.8% depending on the variables considered in the sensitivity
analysis. Therefore, to realize profit potential and many other advantages, the entrant may choose a strategy of
reaching the customer with a solution
HUBUNGAN ANTARA KETERLIBATAN PENGASUHAN AYAH DAN KEMATANGAN EMOSI DENGAN EMPATI PADA GENERASI Z
No full textABSTRAK
HUBUNGAN ANTARA KETERLIBATAN PENGASUHAN
AYAH DAN KEMATANGAN EMOSI DENGAN
EMPATI PADA GENERASI Z
Oleh :
Andri Mahendra
Empati adalah kemampuan seseorang untuk menilai,
membedakan, dan bisa menerima pandangan yang berbeda, yang
mana seorang individu dapat merasakan apa yang dirasakan oleh
orang lain. Empati berkaitan dengan keterlibatan pengasuhan ayah
karena ketika banyaknya peran ayah terlibat dalam pengasuhan maka
itu berdampak terhadap empati sesorang, sama halnya dengan
kematangan emosi pada individu yang memberikan dampak terhadap
empati sehingga semakin tinggi kematangan emosi maka akan
semakin tinggi empati setiap individu.
Populasi dalam penelitian ini adalah mahasiswa aktif yang ada
dikota Bandar Lampung dengan rentang usia 18-23 tahun. Sampel
dalam penelitian ini adalah 349 mahasiswa. Teknik pengambilan data
menggunakan non-probability sampling. Instrument dalam penelitian
ini adalah skala empati α = (0.896) dengan jumlah 23 aitem, skala
keterlibatan pengasuhan ayah α = (0.916) dengan jumlah 29 aitem,
dan skala kematangan emosi α = (0.731) dengan jumlah 16 aitem.
Berdasarkan hasil analisis data menggunakan analisis regresi
berganda dengan software SPSS 25.0 for windows, hasil penelitian
menujukkan bahwa variabel bebas keterlibatan pengasuhan ayah dan
kematangan emosi memberikan sumbangan efektif sebesar 21.8%
dang 78.2% dipengaruhi oleh faktor dari luar penelitian. Hasil
penelitian menunjukkan bahwa terdapat hubungan yang signifikan
antara keterlibatan pengasuhan ayah dan kematangan emosi dengan
empati pada generasi Z, terdapat hubungan antara keterlibatan
pengasuhan ayah dengan empati, dan terdapat hubungan antara
kematangan emosi dengan empati.
Kata kunci : Empati, Keterlibatan Pengasuhan Ayah, Kematangan
Emosi, Generasi Z.
iv
ABSTRACT
Author :
Andri Mahendra
Empathy is a person's ability to judge, distinguish, and be
able to accept different views, in which an individual can feel what
others feel. Empathy is related to the involvement of fathers in
parenting because when many roles of fathers are involved in
parenting, it has an impact on a person's empathy, as well as
emotional maturity in individuals which has an impact on empathy so
that the higher the emotional maturity, the higher the empathy for
each individual.
The population in this study were active students in the city of
Bandar Lampung with an age range of 18-23 years. The sample in this
research was 349 students. The data collection technique uses non�probability sampling. The instruments in this study were the empathy
scale α = (0.896) with 23 items, the father's parenting involvement
scale α = (0.916) with 29 items, and the emotional maturity scale α =
(0.731) with 16 items.
Based on the results of data analysis using multiple regression
analysis with SPSS 25.0 for Windows software, the research results
show that the independent variables of father's parenting involvement
and emotional maturity provide an effective contribution of 21.8% and
78.2% are influenced by factors outside the research. The results of
the research show that there is a significant relationship between
father's parenting involvement and emotional maturity and empathy in
generation Z, there is a relationship between father's parenting
involvement and empathy, and there is a relationship between
emotional maturity and empathy.
Keywords: Empathy, Parenting Involvement, Emotional Maturity,
Generation
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