69 research outputs found
Differentiable solutions of an equation with product of iterates
No full textThe author, together with M. Veerapazham, S. Wang and W. Zhang, considered the existence and uniqueness of continuous solutions to an iterative equation involving the multiplication of iterates in Gopalakrishna et al. (Sci. China Math., to appear). In this paper we continue to investigate this equation for differentiable solutions. Similarly to continuous solutions until Gopalakrishna et al. (Sci. China Math., to appear), no result on differentiable solutions of this equation on non-compact intervals of R has been obtained until now. Although our strategy here is to use the logarithmic conjugation to reduce the equation to the well-known polynomial-like iterative equation, all known results on differentiable solutions of the latter are given on compact intervals. We revisit polynomial-like iterative equations on the whole of R and use the Banach contraction principle to prove the existence and uniqueness of differentiable solutions to our equation on R+. We then technically extend our results on R+ to R- and show that none of the pairs of solutions obtained on R+ and R- can be combined at the origin to obtain even a continuous solution of the equation on the whole R
Decoding the symphony : illuminating the mammalian mitochondrial RNA binding proteome with a novel interactome approach
No full textMitochondria, colloquially known as the "powerhouses of the cell" are crucial for generating cellular energy through a process known as oxidative phosphorylation (OXPHOS). These minute but mighty organelles are distinct and semi-independent entities, containing their own DNA, which is circular in nature. This DNA has the capacity for replication, transcription, and translation, processes collectively referred to as mitochondrial gene expression. Out of the OXPHOS system's peptides, mitochondria encode for 13, with the remainder being produced in the nucleus and then transported into the mitochondria. The complex system of gene expression within mitochondria relies on an intricate interaction among DNA, RNA, and proteins. However, disruptions in this system have been linked to a variety of diseases, such as diabetes, neurodegenerative disorders, and cancer. RNA-binding proteins (RBPs) are essential in regulating various aspects of RNA's lifecycle, including its synthesis, stability, processing, epitranscriptomic modification, translation, and degradation. Yet, the specifics of how these proteins operate remain partially understood.My doctoral research has been devoted to uncovering new RBPs and elucidating their roles in mitochondrial gene expression. Specifically, in paper 1, I introduced a technique named ‘mt-TRIzol-RBP-Ex’ for the efficient and unbiased enrichment of mitochondrial RBPs from HEK293T cells, leading to the identification and partial characterization of a potential novel RBP, C8orf82, in mitochondrial translation. Subsequent papers focused on other RBPs such as C6orf203 (in paper 2) and GTPBP8 (in paper 3), as well as translation initiation factors (in paper 4), revealing their indispensable roles in mitochondrial post-transcriptional pathways. The depletion of these proteins resulted in significant impacts on translation.This doctoral thesis provides a comprehensive review of current knowledge on mitochondrial RBPs and presents both preliminary and published findings on the roles of specific, previously uncharacterized, or novel RBPs in mitochondrial gene expression.List of scientific papersI. Shreekara Gopalakrishna, Ilian Atanassov, Aishe Angeletti Sarshad, Daniel Ben Halevy, Shadi Bavafa, Benedikt Beckmann, Joanna Rorbach†. (2024). Enrichment of mammalian mitochondrial RNA-binding proteins using TRIzolEx reveals novel uncharacterised mt-RNA interacting proteins. †Corresponding author. [Manuscript]II. Shreekara Gopalakrishna*, Sarah F Pearce*, Adam M Dinan, Florian A Rosenberger, Miriam Cipullo, Henrik Spåhr, Anas Khawaja, Camilla Maffezzini, Christoph Freyer, Anna Wredenberg, Ilian Atanassov, Andrew E Firth, Joanna Rorbach†. (2019). C6orf203 is an RNA-binding protein involved in mitochondrial protein synthesis. Nucleic Acids Res. 2019 Sep 26;47(17):9386-9399. *These authors contributed equally. †Corresponding author. https://doi.org/10.1093/nar/gkz684 III. Miriam Cipullo, Genís Valentín Gesé, Shreekara Gopalakrishna, Annika Krueger, Petra Palenkova, Dmitrii Shiriaev, Yong Liu, Jelena Misic, Yu Cai, Minh Nguyen, Abubakar Abdelbagi, Xinping Li, Michal Minczuk, Ilian Attanasov, Martin Hällberg, Joanna Rorbach†. (2024). GTPBP8 is essential for mitoribosome formation in human mitochondria. †Corresponding author. [Submitted]IV. Cristina Remes, Anas Khawaja, Sarah F Pearce, Adam M Dinan, Shreekara Gopalakrishna, Miriam Cipullo, Vasileios Kyriakidis, Jingdian Zhang, Xaquin Castro Dopico, Olessya Yukhnovets, Ilian Atanassov, Andrew E Firth, Barry Cooperman, Joanna Rorbach†. (2023). Translation initiation of leaderless and polycistronic transcripts in mammalian mitochondria. Nucleic Acids Res. 2023 Jan 25;51(2):891-907. †Corresponding author. https://doi.org/10.1093/nar/gkac1233 </p
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An Empirical Study on the Use of Promotion in Hospitals
No full textThe role of marketing and marketing communication in hospitals has grown in the last decade. The need for hospitals to make careful decisions about their marketing communication efforts is mandated, given the changes taking place in the hospital industry. The purpose of this dissertation was to conduct empirical research to determine whether for-profit and non-profit hospitals perceive and utilize promotion as a marketing strategy element. The two steps taken included: identifying important factors considered by hospital administrators and marketing staff in the development of communication messages designed for patients, hospital staff and medical staff; and testing the factors developed and studying the attitudes of hospital personnel toward promotion using a national sample of hospitals. In phase 1, focus group interviews were conducted in a surrogate for-profit hospital and a surrogate non-profit hospital. In phase 2, an original mail questionnaire was used to collect data from a sample of 80 hospitals. A total of 38 hospitals participated, providing 114 usable responses. Test statistics included content analysis, Chi-Square, Pearson correlation coefficient and Analysis of Variance. The results of the focus group study indicated the practice of marketing in hospitals is in its early growth stages and marketing is viewed as nothing more than advertising and public relations. The results of the mail survey indicated that respondents in small for-profit hospitals with 20 to 30 years of experience as professionals, with key decision making authority, are favorably disposed to marketing and marketing communication. It was also found that respondents in large non-profit hospitals are very positive towards marketing. In contrast, respondents in medium and large for-profit hospitals, who are not directly involved in decision making, tend to be less positive towards marketing. The study serves as a basis for future research which may involve, (1) a larger sample frame, (2) hospitals in inner-city and rural areas, (3) investigation of the association between hospital ownership and hospital efficiency, and (4) development of a profile of respondents by title held, in hospitals
Indian Snack Foods
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Effect of Blending Groundnut and Sunflower oils with minor oils on the storage and chemical stability
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Study of Lipolysis in Coconut oil and Preparation of Medium chain Fatty acids
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Study on Frying of Poori using Coconut Oil Blends
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Preparation of Refined Oil from Lipase acted Rice Bran
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Synthesis of Glycerides of short and medium chain fatty acids
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
A Study on Stability of Blended and Interesterified Oils from Niger Seed Oil and Palm Oil
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
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