3,318 research outputs found
Revision of the Melanogaster jaroslavensis group (Diptera: Syrphidae), with description of a new species from Afghanistan
No full textPopov, Grigory V., Prokhorov, Alexey V. (2020): Revision of the Melanogaster jaroslavensis group (Diptera: Syrphidae), with description of a new species from Afghanistan. Zootaxa 4743 (4): 536-552, DOI: 10.11646/zootaxa.4743.4.
FIGURE 3 in Revision of the Melanogaster jaroslavensis group (Diptera: Syrphidae), with description of a new species from Afghanistan
No full textFIGURE 3. Melanogaster jaroslavensis habitat (Sumy Reg., Ukraine).Published as part of Popov, Grigory V. & Prokhorov, Alexey V., 2020, Revision of the Melanogaster jaroslavensis group (Diptera: Syrphidae), with description of a new species from Afghanistan, pp. 536-552 in Zootaxa 4743 (4) on page 540, DOI: 10.11646/zootaxa.4743.4.4, http://zenodo.org/record/369061
FIGURE 1. Melanogaster jaroslavensis. A in Revision of the Melanogaster jaroslavensis group (Diptera: Syrphidae), with description of a new species from Afghanistan
No full textFIGURE 1. Melanogaster jaroslavensis. A: Male, dorsal view; B: Female, dorsal view; C: Male, head and thorax, lateral view; D: Female head, lateral view; E: Male head, frontal view; F: Female head, frontal view. Scale line = 1 mm.Published as part of Popov, Grigory V. & Prokhorov, Alexey V., 2020, Revision of the Melanogaster jaroslavensis group (Diptera: Syrphidae), with description of a new species from Afghanistan, pp. 536-552 in Zootaxa 4743 (4) on page 539, DOI: 10.11646/zootaxa.4743.4.4, http://zenodo.org/record/369061
Lasersäteilyn indusoimien veren punasolujen ja hemoreologisten muutosten vuorovaikutusten erikoispiirteet
No full textAbstractThis thesis reports on studies of the fundamental interaction dynamics of human red blood cells (RBC) by optical tweezers (OT) and the application of the OT-based RBC-investigation protocol in facilitating blood photobiomodulation research at a single-cell level. The motivation for the present study arises from the scientific and clinical significance of examining RBC interaction mechanisms as a model for studying general cell interaction in cytological science. Exploring the phenomenon and mechanism of the photobiomodulation of laser radiation on the rheological properties of RBC has a great potential in the field of laser blood therapy.Interaction dynamics and the role of intercellular interaction time and mutual contact in RBC aggregation and OT-induced disaggregation in autologous plasma are presented as new evidence, clarifying the RBC interaction mechanism. The rheological alterations of RBC induced by laser radiation with various irradiation conditions were thoroughly explored at a single-cell level for the first time to provide a better understanding of the underlying mechanism of photobiomodulation on blood. This study demonstrated the beneficial effects of low-level laser irradiation by a 450-nm wavelength with a radiant exposure below 9.5 J/cm2 of improving RBC deformability and preserving cell shape in a harsh environment. Additionally, irradiated RBC aggregates were easily destroyed by the external influence (i.e., optical force), which could be the flow shearing force in a blood vessel and geometrical resistance by vasculature for in vivo conditions.The current work is important for optimizing the technique of the OT-based RBC evaluation system. It will contribute to the development of effective approaches for improving blood cell viability and blood microcirculation based on the reported photobiomodulation effects.Original papersOriginal papers are not included in the electronic version of the dissertation.Zhu, R., Avsievich, T., Su, X., Bykov, A., Popov, A., & Meglinski, I. (2022). Hemorheological alterations of red blood cells induced by 450-nm and 520-nm laser radiation. Journal of Photochemistry and Photobiology B: Biology, 230, 112438. https://doi.org/10.1016/j.jphotobiol.2022.112438Self-archived versionZhu, R., Avsievich, T., Popov, A., Bykov, A., & Meglinski, I. (2021). In vivo nano-biosensing element of red blood cell-mediated delivery. Biosensors and Bioelectronics, 175, 112845. https://doi.org/10.1016/j.bios.2020.112845Self-archived versionZhu, R., Avsievich, T., Bykov, A., Popov, A., & Meglinski, I. (2019). Influence of pulsed He–Ne laser irradiation on the red blood cell interaction studied by optical tweezers. Micromachines, 10(12), 853. https://doi.org/10.3390/mi10120853Self-archived versionZhu, R., Avsievich, T., Popov, A., & Meglinski, I. (2020). Optical tweezers in studies of red blood cells. Cells, 9(3), 545. https://doi.org/10.3390/cells9030545Self-archived versionZhu, R., Avsievich, T. I., Popov, A., Bykov, A., & Meglinski, I. (2021). In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy. Proceedings of SPIE, Optical Trapping and Optical Micromanipulation XVIII 11798, 1179809. https://doi.org/10.1117/12.2595118Self-archived versionZhu, R., Popov, A., & Meglinski, I. (2020). Probing the red blood cell interaction in individual cell pairs by optical tweezers. Conference on Lasers and Electro-optics, AW3I.4. https://doi.org/10.1364/CLEO_AT.2020.AW3I.4Self-archived versionZhu, R., Avsievich, T., Popov, A., & Meglinski, I. (2019). Influence of interaction time on the red blood cell (dis)aggregation dynamics in vitro studied by optical tweezers. Proceedings of SPIE, Novel Biophotonics Techniques and Applications V 11075, 110750D. https://doi.org/10.1117/12.2526778Self-archived versionTiivistelmäTässä opinnäytetyössä raportoidaan ihmisen punasolujen (RBC) perustavanlaatuisen vuorovaikutusdynamiikan tutkimuksista optisilla pinseteillä (OT) ja OT-pohjaisen RBC-tutkimusprotokollan soveltamisesta veren fotobiomodulaatiotutkimuksen helpottamiseksi yksisolutasolla. Motivaatio nykyiselle tutkimukselle syntyy punasolujen vuorovaikutusmekanismien tutkimisen tieteellisestä ja kliinisestä merkityksestä mallina yleisen soluvuorovaikutuksen tutkimiseen sytologiassa. Kun lasersäteilyn fotobiomodulaation ilmiötä ja mekanismia tutkitaan punasolujen reologisissa ominaisuuksissa, siinä on paljon potentiaalia laserveriterapian alalla.Interaktiodynamiikka ja solunvälisen interaktioajan ja keskinäisen kontaktin rooli punasolujen aggregaatiossa ja OT:n aiheuttamassa hajoamisessa autologisessa plasmassa esitetään uutena todisteena punasolujen yhteisvaikutusmekanismia selventävänä näyttönä. Punasoluissa tapahtuneita, lasersäteilyn erilaisissa valaistusolosuhteissa aiheuttamia reologisia muutoksia tarkasteltiin nyt ensimmäistä kertaa perusteellisesti yksittäisten solujen tasolla, minkä seurauksena saatiin aiempaa parempi ymmärrys veren fotobiomodulaation taustalla olevasta mekanismista. Tutkimus havainnollisti matalaenergisen, teholtaan alle 9,5 J/cm2:n säteilyaltistuksen ja aallonpituudeltaan 450 nm:n lasersäteilyn hyödylliset vaikutukset punasolujen muodonmuutoksen parantamisessa ja solujen kyvyssä säilyttää muotonsa ankarissakin olosuhteissa. Lisäksi säteilytetyt RBC-aggregaatit tuhoutuivat helposti ulkoisen vaikutuksen vaikutuksesta (optinen voima), joka voi olla virtauksen leikkausvoima verisuonessa ja geometrinen vastus verisuonissa in vivo -olosuhteissa.Tutkimuksen saavutukset ovat merkittäviä optimoitaessa optiseen pinsettiin perustuvan punasolujen arviointijärjestelmän tekniikoita. Raportoitujen fotobiomodulaatio vaikutusten kautta työ edistää verisolujen elinkelpoisuuden ja veren mikrovirtauksen parantamiseen tähtäävien tehokkaiden lähestymistapojen kehittämistä.OsajulkaisutOsajulkaisut eivät sisälly väitöskirjan elektroniseen versioon.Zhu, R., Avsievich, T., Su, X., Bykov, A., Popov, A., & Meglinski, I. (2022). Hemorheological alterations of red blood cells induced by 450-nm and 520-nm laser radiation. Journal of Photochemistry and Photobiology B: Biology, 230, 112438. https://doi.org/10.1016/j.jphotobiol.2022.112438Rinnakkaistallennettu versioZhu, R., Avsievich, T., Popov, A., Bykov, A., & Meglinski, I. (2021). In vivo nano-biosensing element of red blood cell-mediated delivery. Biosensors and Bioelectronics, 175, 112845. https://doi.org/10.1016/j.bios.2020.112845Rinnakkaistallennettu versioZhu, R., Avsievich, T., Bykov, A., Popov, A., & Meglinski, I. (2019). Influence of pulsed He–Ne laser irradiation on the red blood cell interaction studied by optical tweezers. Micromachines, 10(12), 853. https://doi.org/10.3390/mi10120853Rinnakkaistallennettu versioZhu, R., Avsievich, T., Popov, A., & Meglinski, I. (2020). Optical tweezers in studies of red blood cells. Cells, 9(3), 545. https://doi.org/10.3390/cells9030545Rinnakkaistallennettu versioZhu, R., Avsievich, T. I., Popov, A., Bykov, A., & Meglinski, I. (2021). In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy. Proceedings of SPIE, Optical Trapping and Optical Micromanipulation XVIII 11798, 1179809. https://doi.org/10.1117/12.2595118Rinnakkaistallennettu versioZhu, R., Popov, A., & Meglinski, I. (2020). Probing the red blood cell interaction in individual cell pairs by optical tweezers. Conference on Lasers and Electro-optics, AW3I.4. https://doi.org/10.1364/CLEO_AT.2020.AW3I.4Rinnakkaistallennettu versioZhu, R., Avsievich, T., Popov, A., & Meglinski, I. (2019). Influence of interaction time on the red blood cell (dis)aggregation dynamics in vitro studied by optical tweezers. Proceedings of SPIE, Novel Biophotonics Techniques and Applications V 11075, 110750D. https://doi.org/10.1117/12.2526778Rinnakkaistallennettu versioAcademic dissertation to be presented with the assent of the Doctoral Programme Committee of Information Technology and Electrical Engineering of the University of Oulu for public defence in the OP auditorium (L10), Linnanmaa, on 30 June 2022, at 12 noonAbstract
This thesis reports on studies of the fundamental interaction dynamics of human red blood cells (RBC) by optical tweezers (OT) and the application of the OT-based RBC-investigation protocol in facilitating blood photobiomodulation research at a single-cell level. The motivation for the present study arises from the scientific and clinical significance of examining RBC interaction mechanisms as a model for studying general cell interaction in cytological science. Exploring the phenomenon and mechanism of the photobiomodulation of laser radiation on the rheological properties of RBC has a great potential in the field of laser blood therapy.
Interaction dynamics and the role of intercellular interaction time and mutual contact in RBC aggregation and OT-induced disaggregation in autologous plasma are presented as new evidence, clarifying the RBC interaction mechanism. The rheological alterations of RBC induced by laser radiation with various irradiation conditions were thoroughly explored at a single-cell level for the first time to provide a better understanding of the underlying mechanism of photobiomodulation on blood. This study demonstrated the beneficial effects of low-level laser irradiation by a 450-nm wavelength with a radiant exposure below 9.5 J/cm2 of improving RBC deformability and preserving cell shape in a harsh environment. Additionally, irradiated RBC aggregates were easily destroyed by the external influence (i.e., optical force), which could be the flow shearing force in a blood vessel and geometrical resistance by vasculature for in vivo conditions.
The current work is important for optimizing the technique of the OT-based RBC evaluation system. It will contribute to the development of effective approaches for improving blood cell viability and blood microcirculation based on the reported photobiomodulation effects.Tiivistelmä
Tässä opinnäytetyössä raportoidaan ihmisen punasolujen (RBC) perustavanlaatuisen vuorovaikutusdynamiikan tutkimuksista optisilla pinseteillä (OT) ja OT-pohjaisen RBC-tutkimusprotokollan soveltamisesta veren fotobiomodulaatiotutkimuksen helpottamiseksi yksisolutasolla. Motivaatio nykyiselle tutkimukselle syntyy punasolujen vuorovaikutusmekanismien tutkimisen tieteellisestä ja kliinisestä merkityksestä mallina yleisen soluvuorovaikutuksen tutkimiseen sytologiassa. Kun lasersäteilyn fotobiomodulaation ilmiötä ja mekanismia tutkitaan punasolujen reologisissa ominaisuuksissa, siinä on paljon potentiaalia laserveriterapian alalla.
Interaktiodynamiikka ja solunvälisen interaktioajan ja keskinäisen kontaktin rooli punasolujen aggregaatiossa ja OT:n aiheuttamassa hajoamisessa autologisessa plasmassa esitetään uutena todisteena punasolujen yhteisvaikutusmekanismia selventävänä näyttönä. Punasoluissa tapahtuneita, lasersäteilyn erilaisissa valaistusolosuhteissa aiheuttamia reologisia muutoksia tarkasteltiin nyt ensimmäistä kertaa perusteellisesti yksittäisten solujen tasolla, minkä seurauksena saatiin aiempaa parempi ymmärrys veren fotobiomodulaation taustalla olevasta mekanismista. Tutkimus havainnollisti matalaenergisen, teholtaan alle 9,5 J/cm2:n säteilyaltistuksen ja aallonpituudeltaan 450 nm:n lasersäteilyn hyödylliset vaikutukset punasolujen muodonmuutoksen parantamisessa ja solujen kyvyssä säilyttää muotonsa ankarissakin olosuhteissa. Lisäksi säteilytetyt RBC-aggregaatit tuhoutuivat helposti ulkoisen vaikutuksen vaikutuksesta (optinen voima), joka voi olla virtauksen leikkausvoima verisuonessa ja geometrinen vastus verisuonissa in vivo -olosuhteissa.
Tutkimuksen saavutukset ovat merkittäviä optimoitaessa optiseen pinsettiin perustuvan punasolujen arviointijärjestelmän tekniikoita. Raportoitujen fotobiomodulaatio vaikutusten kautta työ edistää verisolujen elinkelpoisuuden ja veren mikrovirtauksen parantamiseen tähtäävien tehokkaiden lähestymistapojen kehittämistä
Punasolut ja uudet nanomateriaalit: kohti nanoturvallisuutta ja nanolääketiedettä
No full textAbstractNanomaterials are an essential part of modern life due to their extensive use in industrial and commercial products and future personalized medicine. In recent years, new nanoparticles (NPs) have been introduced in bioimaging, diagnostics, drug delivery, and therapy. Along with the production growth of NPs, concerns about NPs safety for human health have been raised. As blood is an inevitable target for NP-based pharmaceutics at systemic NP-based drug administration, the investigation of the poorly understood effects of NPs on blood properties is of high demand.The present thesis focuses on the assessment of commercial and novel synthesized NPs towards the haemorheologica l properties of the main blood cellular component — red blood cells (RBCs). In the research for this thesis, RBC morphology, deformability and mutual interactions were examined at non-haemolytic concentrations of NPs. A revolutionary optical tweezers technique revealed subtle effects of indirect toxicity towards mutual RBCs interactions and deformability. Further, additional conventional optical microscopy analysis revealed the influence of NPs on RBC interactions on a multicellular level, while scanning electron microscopy (SEM) enabled high-resolution monitoring of RBCs surfaces and morphological alterations triggered by NPs. Another aim of the study addressed the unclear mechanism behind RBC interactions. Experimental evidence of the definitive role of the size and proportion of macromolecules in RBC interactions was provided. The mixture of natural polymer dextran mimicking plasma protein composition induced an RBC interaction mode similar to one observed in blood plasma. Thus, the new hybrid model combining “cross-bridges” and “depletion” effects was proposed.The reported findings contribute to the fundamental understanding of RBC interactions and can help to facilitate the design and clinical validation of polymer-based plasma expanders and novel NPs for safe and beneficial use.Original papersOriginal papers are not included in the electronic version of the dissertation.Avsievich, T., Zhu, R., Popov, A., Bykov, A., & Meglinski, I. (2022). Blood–nanomaterials interactions. In A. Denizli. T. Nguyen, R. Mariappan, M. Feroz Alam, & K. Khaliqur Rahman (Eds.), Nanotechnology for Hematology, Blood Transfusion, and Artificial Blood (pp. 1–40). Elsevier. https://doi.org/10.1016/B978-0-12-823971-1.00002-7Avsievich, T., Zhu, R., Popov, A., Bykov, A., & Meglinski, I. (2020). The advancement of blood cell research by optical tweezers. Reviews in Physics, 5, 100043. https://doi.org/10.1016/j.revip.2020.100043Self-archived versionAvsievich, T., Popov, A., Bykov, A., & Meglinski, I. (2018). Mutual interaction of red blood cells assessed by optical tweezers and scanning electron microscopy imaging. Optics Letters, 43(16), 3921–3924. https://doi.org/10.1364/OL.43.003921Self-archived versionAvsievich, T., Tarakanchikova, Y., Zhu, R., Popov, A., Bykov, A., Skovorodkin, I., Vainio, S., & Meglinski, I. (2019). Impact of nanocapsules on red blood cells interplay jointly assessed by optical tweezers and microscopy. Micromachines, 11(1), 19. https://doi.org/10.3390/mi11010019Self-archived versionAvsievich, T., Tarakanchikova, Y. V., Popov, A., Karmenyan, A., Bykov, A., & Meglinski, I. (2019). Combined use of optical tweezers and scanning electron microscopy to reveal influence of nanoparticles on red blood cells interactions. In A. Amelink, & S. K. Nadkarni (Eds.), Novel Biophotonics Techniques and Applications V (110750F). Proceedings of SPIE, 11075. https://doi.org/10.1117/12.2526730Self-archived versionAvsievich, T., Popov, A., Bykov, A., & Meglinski, I. (2019). Mutual interaction of red blood cells influenced by nanoparticles. Scientific Reports, 9(1), 5147. https://doi.org/10.1038/s41598-019-41643-xSelf-archived versionAvsievich, T., Zhu, R., Popov, A., Yatskovskiy, A., Popov, A. A., Tikhonowsky, G., Pastukhov, A., Klimentov, S., Bykov, A., Kabashin, A., & Meglinski, I. (2022). Hemorheological alterations of erythrocytes induced by plasmonic nanoparticles. Manuscript submitted for publication.TiivistelmäNanomateriaalit ovat olennainen osa nykyaikaista elämäntapaa, sillä niitä käytetään laajasti teollisissa ja kaupallisissa tuotteissa sekä tulevaisuuden yksilöllisessä lääketieteessä. Viime vuosina uusia nanopartikkeleita (NP) on otettu käyttöön biolääketieteellisessä kuvantamisessa ja diagnostiikassa sekä lääkkeiden antamisessa ja terapiassa. NP:iden tuotannon kasvun myötä on noussut esiin huoli niiden turvallisuudesta ihmisten terveydelle. Koska veri on väistämätön kohde NP-pohjaisille lääkkeille systeemisessä NP-pohjaisessa lääkkeen annossa, on nanopartikkelien vähän tutkittujen, veren ominaisuuksiin kohdistuvien vaikutusten selvittämiselle suuri tarve.Kyseinen opinnäytetyö keskittyy kaupallisten ja uusien syntetisoitujen nanaopartikkelien arviointiin verisolujen pääkomponentin eli punasolujen (RBC) hemorheologisten ominaisuuksien osalta. Tämän työn tutkimuksessa tarkasteltiin punasolujen morfologiaa, muodonmuutoksia ja keskinäisiä vuorovaikutuksia ei-hemolyyttisissä NP-pitoisuuksissa. Vallankumouksellinen optinen pinsettitekniikka paljasti epäsuoran myrkyllisyyden hienovaraiset vaikutukset punasolujen keskinäiseen vuorovaikutukseen ja muodonmuutokseen. Lisäksi tavanomainen optinen mikroskopia-analyysi paljasti NP:iden ja RBC:n vuorovaikutukset monisoluisella tasolla, kun taas pyyhkäisyelektronimikroskopia (SEM) mahdollisti punasolujen pintojen ja NP:iden laukaisemien morfologisten muutosten korkearesoluutioisen seurannan. Tutkimuksen toisena tavoitteena oli selvittää punasolujen vuorovaikutuksen taustalla olevaa epäselvää mekanismia. Työssä esitettiin kokeellisia todisteita makromolekyylien koon ja osuuden lopullisesta roolista punasolujen vuorovaikutuksessa. Plasmaproteiinikoostumusta jäljittelevän luonnollisen polymeerin dekstraanin seos indusoi punasolujen vuorovaikutusmuodon, joka on samanlainen kuin veriplasmassa havaittu. Siten työssä ehdotettiin uutta "ristisiltoja" ja "tyhjennystä” yhdistävää hybridimallia.Raportoidut löydökset auttavat ymmärtämään punasolujen vuorovaikutuksia ja voivat helpottaa polymeeripohjaisten plasmalaajenninten ja uusien nanopartikkelien suunnittelua sekä niiden kliinistä validointia turvallisen ja hyödyllisen käytön mahdollistamiseksi.OsajulkaisutOsajulkaisut eivät sisälly väitöskirjan elektroniseen versioon.Avsievich, T., Zhu, R., Popov, A., Bykov, A., & Meglinski, I. (2022). Blood–nanomaterials interactions. In A. Denizli. T. Nguyen, R. Mariappan, M. Feroz Alam, & K. Khaliqur Rahman (Eds.), Nanotechnology for Hematology, Blood Transfusion, and Artificial Blood (pp. 1–40). Elsevier. https://doi.org/10.1016/B978-0-12-823971-1.00002-7Avsievich, T., Zhu, R., Popov, A., Bykov, A., & Meglinski, I. (2020). The advancement of blood cell research by optical tweezers. Reviews in Physics, 5, 100043. https://doi.org/10.1016/j.revip.2020.100043Rinnakkaistallennettu versioAvsievich, T., Popov, A., Bykov, A., & Meglinski, I. (2018). Mutual interaction of red blood cells assessed by optical tweezers and scanning electron microscopy imaging. Optics Letters, 43(16), 3921–3924. https://doi.org/10.1364/OL.43.003921Rinnakkaistallennettu versioAvsievich, T., Tarakanchikova, Y., Zhu, R., Popov, A., Bykov, A., Skovorodkin, I., Vainio, S., & Meglinski, I. (2019). Impact of nanocapsules on red blood cells interplay jointly assessed by optical tweezers and microscopy. Micromachines, 11(1), 19. https://doi.org/10.3390/mi11010019Rinnakkaistallennettu versioAvsievich, T., Tarakanchikova, Y. V., Popov, A., Karmenyan, A., Bykov, A., & Meglinski, I. (2019). Combined use of optical tweezers and scanning electron microscopy to reveal influence of nanoparticles on red blood cells interactions. In A. Amelink, & S. K. Nadkarni (Eds.), Novel Biophotonics Techniques and Applications V (110750F). Proceedings of SPIE, 11075. https://doi.org/10.1117/12.2526730Rinnakkaistallennettu versioAvsievich, T., Popov, A., Bykov, A., & Meglinski, I. (2019). Mutual interaction of red blood cells influenced by nanoparticles. Scientific Reports, 9(1), 5147. https://doi.org/10.1038/s41598-019-41643-xRinnakkaistallennettu versioAvsievich, T., Zhu, R., Popov, A., Yatskovskiy, A., Popov, A. A., Tikhonowsky, G., Pastukhov, A., Klimentov, S., Bykov, A., Kabashin, A., & Meglinski, I. (2022). Hemorheological alterations of erythrocytes induced by plasmonic nanoparticles. Manuscript submitted for publication.Academic dissertation to be presented with the assent of the Doctoral Programme Committee of Information Technology and Electrical Engineering of the University of Oulu for public defence in the OP auditorium (L10), Linnanmaa, on 10 October 2022, at 12 noonAbstract
Nanomaterials are an essential part of modern life due to their extensive use in industrial and commercial products and future personalized medicine. In recent years, new nanoparticles (NPs) have been introduced in bioimaging, diagnostics, drug delivery, and therapy. Along with the production growth of NPs, concerns about NPs safety for human health have been raised. As blood is an inevitable target for NP-based pharmaceutics at systemic NP-based drug administration, the investigation of the poorly understood effects of NPs on blood properties is of high demand.
The present thesis focuses on the assessment of commercial and novel synthesized NPs towards the haemorheologica l properties of the main blood cellular component — red blood cells (RBCs). In the research for this thesis, RBC morphology, deformability and mutual interactions were examined at non-haemolytic concentrations of NPs. A revolutionary optical tweezers technique revealed subtle effects of indirect toxicity towards mutual RBCs interactions and deformability. Further, additional conventional optical microscopy analysis revealed the influence of NPs on RBC interactions on a multicellular level, while scanning electron microscopy (SEM) enabled high-resolution monitoring of RBCs surfaces and morphological alterations triggered by NPs. Another aim of the study addressed the unclear mechanism behind RBC interactions. Experimental evidence of the definitive role of the size and proportion of macromolecules in RBC interactions was provided. The mixture of natural polymer dextran mimicking plasma protein composition induced an RBC interaction mode similar to one observed in blood plasma. Thus, the new hybrid model combining “cross-bridges” and “depletion” effects was proposed.
The reported findings contribute to the fundamental understanding of RBC interactions and can help to facilitate the design and clinical validation of polymer-based plasma expanders and novel NPs for safe and beneficial use.Tiivistelmä
Nanomateriaalit ovat olennainen osa nykyaikaista elämäntapaa, sillä niitä käytetään laajasti teollisissa ja kaupallisissa tuotteissa sekä tulevaisuuden yksilöllisessä lääketieteessä. Viime vuosina uusia nanopartikkeleita (NP) on otettu käyttöön biolääketieteellisessä kuvantamisessa ja diagnostiikassa sekä lääkkeiden antamisessa ja terapiassa. NP:iden tuotannon kasvun myötä on noussut esiin huoli niiden turvallisuudesta ihmisten terveydelle. Koska veri on väistämätön kohde NP-pohjaisille lääkkeille systeemisessä NP-pohjaisessa lääkkeen annossa, on nanopartikkelien vähän tutkittujen, veren ominaisuuksiin kohdistuvien vaikutusten selvittämiselle suuri tarve.
Kyseinen opinnäytetyö keskittyy kaupallisten ja uusien syntetisoitujen nanaopartikkelien arviointiin verisolujen pääkomponentin eli punasolujen (RBC) hemorheologisten ominaisuuksien osalta. Tämän työn tutkimuksessa tarkasteltiin punasolujen morfologiaa, muodonmuutoksia ja keskinäisiä vuorovaikutuksia ei-hemolyyttisissä NP-pitoisuuksissa. Vallankumouksellinen optinen pinsettitekniikka paljasti epäsuoran myrkyllisyyden hienovaraiset vaikutukset punasolujen keskinäiseen vuorovaikutukseen ja muodonmuutokseen. Lisäksi tavanomainen optinen mikroskopia-analyysi paljasti NP:iden ja RBC:n vuorovaikutukset monisoluisella tasolla, kun taas pyyhkäisyelektronimikroskopia (SEM) mahdollisti punasolujen pintojen ja NP:iden laukaisemien morfologisten muutosten korkearesoluutioisen seurannan. Tutkimuksen toisena tavoitteena oli selvittää punasolujen vuorovaikutuksen taustalla olevaa epäselvää mekanismia. Työssä esitettiin kokeellisia todisteita makromolekyylien koon ja osuuden lopullisesta roolista punasolujen vuorovaikutuksessa. Plasmaproteiinikoostumusta jäljittelevän luonnollisen polymeerin dekstraanin seos indusoi punasolujen vuorovaikutusmuodon, joka on samanlainen kuin veriplasmassa havaittu. Siten työssä ehdotettiin uutta "ristisiltoja" ja "tyhjennystä” yhdistävää hybridimallia.
Raportoidut löydökset auttavat ymmärtämään punasolujen vuorovaikutuksia ja voivat helpottaa polymeeripohjaisten plasmalaajenninten ja uusien nanopartikkelien suunnittelua sekä niiden kliinistä validointia turvallisen ja hyödyllisen käytön mahdollistamiseksi
Multilayered polyelectrolyte assemblies as delivery system for biomedical applications
Get PDFAbstractGene therapy is a rapidly developing medical field, which focuses on the utilization of therapeutic delivery of recombinant nucleic acids into a patient’s cells to treat or prevent a broad spectrum of diseases. However, several important obstacles remain before its wide introduction into clinical application can be implemented. One of the biggest bottlenecks is a lack of efficient and safe delivery technologies, particularly, for in vivo distribution. Additionally, standard requirements for carriers are still an open question (safety, minimal/absent toxicity and immunogenicity, sufficient packaging capacity, targeting, straight and low-cost large-scale Good Manufacturing Practice (GMP) production). Therefore, a growing variety of non-viral delivery platforms represent a promising alternative. Nanotechnology opens new possibilities for resolving biomedical issues. Polymer and hybrid micro- and core–shell nanoparticles are currently under development as a platform for safe and efficient gene delivery.The present thesis describes the development of new safety gene delivery system based on polymer nanoparticles. The results show that nucleic acids (DNA/RNA) can be successfully imbedded into the nanoparticle structures and delivered to various types of cells. For the characterization of the biocompatibility of nanoparticles in vitro, two optical methods were considered. Compatibility with red blood cells (important for intravenous delivery) was assessed using optical tweezers. Capsule biodistribution in vivo was studied with fluorescence spectroscopy and a radiolabeling technique. The data and experience gained from this research open new prospects in the fields of delivery systems areas, gene therapy, and diagnostics in vivo and new possibilities for future clinical applications.Original papersOriginal papers are not included in the electronic version of the dissertation.Tarakanchikova, Y., Stelmashchuk, O., Seryogina, E., Piavchenko, G., Zherebtsov, E., Dunaev, A., Popov, A., & Meglinski, I. (2018). Allocation of rhodamine-loaded nanocapsules from blood circulatory system to adjacent tissues assessed in vivo by fluorescence spectroscopy. Laser Physics Letters, 15(10), 105601. https://doi.org/10.1088/1612-202X/aad857Self-archived versionTarakanchikova, Y., Seryogina, E., Piavchenko, G., Dunaev, A., Popov, A., Meglinsky, I., Stelmashchuk, O., & Zherebtsov, E. (2018). Noninvasive control of rhodamine-loaded capsules distribution in vivo. In V. V. Tuchin, D. E. Postnov, E. A. Genina, & V. L. Derbov (Eds.), Saratov Fall Meeting 2017: Optical Technologies in Biophysics and Medicine XIX (p. 179). SPIE. https://doi.org/10.1117/12.2315773Self-archived versionAvsievich, T., Tarakanchikova, Y., Zhu, R., Popov, A., Bykov, A., Skovorodkin, I., Vainio, S., & Meglinski, I. (2019). Impact of nanocapsules on red blood cells interplay jointly assessed by optical tweezers and microscopy. Micromachines, 11(1), 19. https://doi.org/10.3390/mi11010019Self-archived versionAvsievich, T., Tarakanchikova, Y. V., Popov, A., Karmenyan, A., Bykov, A., & Meglinski, I. (2019). Combined use of optical tweezers and scanning electron microscopy to reveal influence of nanoparticles on red blood cells interactions. In A. Amelink & S. K. Nadkarni (Eds.), Novel Biophotonics Techniques and Applications V (p. 14). SPIE. https://doi.org/10.1117/12.2526730Self-archived versionTarakanchikova, Y., Alzubi, J., Pennucci, V., Follo, M., Kochergin, B., Muslimov, A., Skovorodkin, I., Vainio, S., Antipina, M. N., Atkin, V., Popov, A., Meglinski, I., Cathomen, T., Cornu, T. I., Gorin, D. A., Sukhorukov, G. B., & Nazarenko, I. (2020). Biodegradable nanocarriers resembling extracellular vesicles deliver genetic material with the highest efficiency to various cell types. Small, 16(3), 1904880. https://doi.org/10.1002/smll.201904880Self-archived versionTarakanchikova, Y., Muslimov, A., Sergeev, I., Lepik, K., Yolshin, N., Goncharenko, A., Vasilyev, K., Eliseev, I., Bukatin, A., Sergeev, V., Pavlov, S., Popov, A., Meglinski, I., Afanasiev, B., Parakhonskiy, B., Sukhorukov, G., & Gorin, D. (2020). A highly efficient and safe gene delivery platform based on polyelectrolyte core–shell nanoparticles for hard-to-transfect clinically relevant cell types. Journal of Materials Chemistry B, 8(41), 9576–9588. https://doi.org/10.1039/D0TB01359ETiivistelmäGeeniterapia on nopeasti kehittyvä lääketieteellinen ala, joka keskittyy rekombinanttisten nukleiinihappojen terapeuttisen annon hyödyntämiseen potilaan soluihin laajan kirjon tautien hoitamiseksi tai ehkäisemiseksi. On kuitenkin olemassa useita tärkeitä esteitä, ennen kuin sen laajaa käyttöönottoa kliinisessä sovelluksessa voidaan toteuttaa. Yksi suurimmista pullonkauloista on tehokkaiden ja turvallisten jakelutekniikoiden puute etenkin in vivo -jakelussa. Myös kiistanalainen vakiovaatimukset operaattoreille ovat edelleen avoin ongelma (turvallisuus, vähäinen / puuttuva myrkyllisyys ja immunogeenisuus, riittävä pakkauskapasiteetti, kohdennus, suora ja edullinen laajamittainen GMP-tuotanto). Siksi kasvava valikoima ei-viraalisia jakelualustoja on lupaava vaihtoehto. Nanoteknologia avaa uuden mahdollisuuden ratkaista biolääketieteelliset kysymykset. Polymeerisiä ja hybridimikro- ja ydin-kuori-nanohiukkasia kehitetään parhaillaan turvallisen ja tehokkaan geeninsiirron alustana.Tässä opinnäytetyössä kuvataan polymeerisiin nanohiukkasiin perustuvan uuden turvallisuusgeenin kuljetusjärjestelmän kehittäminen. Tulokset osoittivat, että nukleiinihapot (DNA / RNA) voidaan upottaa onnistuneesti nanohiukkasten rakenteeseen ja toimittaa erityyppisiin soluihin. Nanohiukkasten biologisen yhteensopivuuden in vitro karakterisoimiseksi otettiin huomioon kaksi optista menetelmää. Yhteensopivuus punasolujen kanssa (tärkeä laskimoon annettaessa) arvioitiin optisilla pinseteillä. Kapselien biologinen jakautuminen in vivo mitattiin ja tutkittiin fluoresenssispektroskopialla ja radioleimaustekniikalla. Tästä tutkimuksesta saadut tiedot ja kokemukset avaavat uusia näkymiä jakelujärjestelmiin, geeniterapiaan ja diagnostiikkaan in vivo ja avaavat uusia mahdollisuuksia tulevassa kliinisessä sovelluksessa.OsajulkaisutOsajulkaisut eivät sisälly väitöskirjan elektroniseen versioon.Tarakanchikova, Y., Stelmashchuk, O., Seryogina, E., Piavchenko, G., Zherebtsov, E., Dunaev, A., Popov, A., & Meglinski, I. (2018). Allocation of rhodamine-loaded nanocapsules from blood circulatory system to adjacent tissues assessed in vivo by fluorescence spectroscopy. Laser Physics Letters, 15(10), 105601. https://doi.org/10.1088/1612-202X/aad857Rinnakkaistallennettu versioTarakanchikova, Y., Seryogina, E., Piavchenko, G., Dunaev, A., Popov, A., Meglinsky, I., Stelmashchuk, O., & Zherebtsov, E. (2018). Noninvasive control of rhodamine-loaded capsules distribution in vivo. In V. V. Tuchin, D. E. Postnov, E. A. Genina, & V. L. Derbov (Eds.), Saratov Fall Meeting 2017: Optical Technologies in Biophysics and Medicine XIX (p. 179). SPIE. https://doi.org/10.1117/12.2315773Rinnakkaistallennettu versioAvsievich, T., Tarakanchikova, Y., Zhu, R., Popov, A., Bykov, A., Skovorodkin, I., Vainio, S., & Meglinski, I. (2019). Impact of nanocapsules on red blood cells interplay jointly assessed by optical tweezers and microscopy. Micromachines, 11(1), 19. https://doi.org/10.3390/mi11010019Rinnakkaistallennettu versioAvsievich, T., Tarakanchikova, Y. V., Popov, A., Karmenyan, A., Bykov, A., & Meglinski, I. (2019). Combined use of optical tweezers and scanning electron microscopy to reveal influence of nanoparticles on red blood cells interactions. In A. Amelink & S. K. Nadkarni (Eds.), Novel Biophotonics Techniques and Applications V (p. 14). SPIE. https://doi.org/10.1117/12.2526730Rinnakkaistallennettu versioTarakanchikova, Y., Alzubi, J., Pennucci, V., Follo, M., Kochergin, B., Muslimov, A., Skovorodkin, I., Vainio, S., Antipina, M. N., Atkin, V., Popov, A., Meglinski, I., Cathomen, T., Cornu, T. I., Gorin, D. A., Sukhorukov, G. B., & Nazarenko, I. (2020). Biodegradable nanocarriers resembling extracellular vesicles deliver genetic material with the highest efficiency to various cell types. Small, 16(3), 1904880. https://doi.org/10.1002/smll.201904880Rinnakkaistallennettu versioTarakanchikova, Y., Muslimov, A., Sergeev, I., Lepik, K., Yolshin, N., Goncharenko, A., Vasilyev, K., Eliseev, I., Bukatin, A., Sergeev, V., Pavlov, S., Popov, A., Meglinski, I., Afanasiev, B., Parakhonskiy, B., Sukhorukov, G., & Gorin, D. (2020). A highly efficient and safe gene delivery platform based on polyelectrolyte core–shell nanoparticles for hard-to-transfect clinically relevant cell types. Journal of Materials Chemistry B, 8(41), 9576–9588. https://doi.org/10.1039/D0TB01359EAcademic dissertation to be presented with the assent of the Doctoral Training Committee of Information Technology and Electrical Engineering of the University of Oulu for public defence in the OP auditorium (L10), Linnanmaa, on 3 November 2021, at 12 noonAbstract
Gene therapy is a rapidly developing medical field, which focuses on the utilization of therapeutic delivery of recombinant nucleic acids into a patient’s cells to treat or prevent a broad spectrum of diseases. However, several important obstacles remain before its wide introduction into clinical application can be implemented. One of the biggest bottlenecks is a lack of efficient and safe delivery technologies, particularly, for in vivo distribution. Additionally, standard requirements for carriers are still an open question (safety, minimal/absent toxicity and immunogenicity, sufficient packaging capacity, targeting, straight and low-cost large-scale Good Manufacturing Practice (GMP) production). Therefore, a growing variety of non-viral delivery platforms represent a promising alternative. Nanotechnology opens new possibilities for resolving biomedical issues. Polymer and hybrid micro- and core–shell nanoparticles are currently under development as a platform for safe and efficient gene delivery.
The present thesis describes the development of new safety gene delivery system based on polymer nanoparticles. The results show that nucleic acids (DNA/RNA) can be successfully imbedded into the nanoparticle structures and delivered to various types of cells. For the characterization of the biocompatibility of nanoparticles in vitro, two optical methods were considered. Compatibility with red blood cells (important for intravenous delivery) was assessed using optical tweezers. Capsule biodistribution in vivo was studied with fluorescence spectroscopy and a radiolabeling technique. The data and experience gained from this research open new prospects in the fields of delivery systems areas, gene therapy, and diagnostics in vivo and new possibilities for future clinical applications.Tiivistelmä
Geeniterapia on nopeasti kehittyvä lääketieteellinen ala, joka keskittyy rekombinanttisten nukleiinihappojen terapeuttisen annon hyödyntämiseen potilaan soluihin laajan kirjon tautien hoitamiseksi tai ehkäisemiseksi. On kuitenkin olemassa useita tärkeitä esteitä, ennen kuin sen laajaa käyttöönottoa kliinisessä sovelluksessa voidaan toteuttaa. Yksi suurimmista pullonkauloista on tehokkaiden ja turvallisten jakelutekniikoiden puute etenkin in vivo -jakelussa. Myös kiistanalainen vakiovaatimukset operaattoreille ovat edelleen avoin ongelma (turvallisuus, vähäinen / puuttuva myrkyllisyys ja immunogeenisuus, riittävä pakkauskapasiteetti, kohdennus, suora ja edullinen laajamittainen GMP-tuotanto). Siksi kasvava valikoima ei-viraalisia jakelualustoja on lupaava vaihtoehto. Nanoteknologia avaa uuden mahdollisuuden ratkaista biolääketieteelliset kysymykset. Polymeerisiä ja hybridimikro- ja ydin-kuori-nanohiukkasia kehitetään parhaillaan turvallisen ja tehokkaan geeninsiirron alustana.
Tässä opinnäytetyössä kuvataan polymeerisiin nanohiukkasiin perustuvan uuden turvallisuusgeenin kuljetusjärjestelmän kehittäminen. Tulokset osoittivat, että nukleiinihapot (DNA / RNA) voidaan upottaa onnistuneesti nanohiukkasten rakenteeseen ja toimittaa erityyppisiin soluihin. Nanohiukkasten biologisen yhteensopivuuden in vitro karakterisoimiseksi otettiin huomioon kaksi optista menetelmää. Yhteensopivuus punasolujen kanssa (tärkeä laskimoon annettaessa) arvioitiin optisilla pinseteillä. Kapselien biologinen jakautuminen in vivo mitattiin ja tutkittiin fluoresenssispektroskopialla ja radioleimaustekniikalla. Tästä tutkimuksesta saadut tiedot ja kokemukset avaavat uusia näkymiä jakelujärjestelmiin, geeniterapiaan ja diagnostiikkaan in vivo ja avaavat uusia mahdollisuuksia tulevassa kliinisessä sovelluksessa
Melanogaster raccoon Popov & Prokhorov 2020, sp. nov.
No full textMelanogaster raccoon sp. nov. Figs 6 A–J; 7–9 Type material. Holotype. ♂ [Afghanistan, Bamyan Prov., Band-e Amir, alt 2900 m, 26.VI.2016, Yu. Skrylnik]. Paratypes. No. 1: ♂ [Afghanistan, vic. of Kabul, Paghman, alt 2700 m, 2.VI.2010, O. Pack leg.]; No. 2: ♂ [Af- ghanistan, Band-e Amir, Bamyan Province, alt 3200 m, 25.V.2010, O. Pack leg.]; No. 3 & 4: 2 ♂♂ [Afghani- stan, Kabul Prov., Paghman, alt 2500–2600 m, 9.VI.2016, O. Pak leg.]; No. 5 & 6: 2 ♀♀ [Afghanistan, Sha-tu pass, alt 3100–3200 m, 18.VI.2016, Yu. Skrylnik leg.]; No. 7: ♀ [Afghanistan, Kabul Prov., Paghman, alt 2400 m, 30.VI.2013, Yu. Skrylnik leg.]. Diagnosis. Small-sized robust species with body length 6.0– 7.5 mm. Melanogaster raccoon sp. nov. male can be separated from males of the other three species by the virtual absence of a facial tubercle. Melanogaster raccoon sp. nov. is most similar to M. tadzhikorum, but can be separated from it by: frons covered with pale pile only; face and frons broader; emarginated surstyli at the apex in ventral view. Melanogaster raccoon sp. nov. female differs from females of the other three species by the absence of transverse furrows on the frons. Melanogaster raccoon sp. nov. can be distinguished both from M. jaroslavensis and M. kirgisorum by: halter colour (light greyish-brown stem and dark knob in M. raccoon sp. nov., but entirely yellow in those species); frons and face widths (clearly wider in M. raccoon sp. nov.); surstyli short and emarginated apically in ventral view. Hypopygium very similar to M. tadzhikorum. Hypandrium similar to one of the M. hirtella species-group, epandrium typical for the M. nuda species-group (Maibach et al., 1994 a; Kassebeer, 1999 a). Generally, M. raccoon sp. nov. can be easily separated from remaining species of this group by the combination of the body pile pale without dark ones on male frons, the almost flat face in male, the absence of frontal transverse furrows in female, and surstyli both short and emarginated apically in ventral view. Description. MALE (Figs 6A, C–G; 7). Body length: 6.5–7.5 mm. Head (Fig. 6E, F). Eyes bare, holoptic; frons slightly swollen, shining, upper half with dense and long yellowish-white or only white pile; face and frons broad, ratio of the maximum head width to the frons width at the level of the antennal base 1.9–2.0. Frons shiny, apart from pollinose stripe between eyes from antennal base to facial tubercle or almost completely pollinose; facial pile whitish; facial tubercle very small, weakly protruded, almost absent; antenna brownish-black to black including bare arista; ocelli nearly equidistant but the distance between the posterior ocelli slightly greater; vertex and occiput white pilose. Thorax (Fig. 6A, C). Scutum and scutellum shiny black with weak bluish shine, not pollinose, finely punctate, covered with long whitish pile, on posterior margin of scutellum pile longer; sides of thorax shiny, whitish pilose (postpronotum, posterior anepisternum, anepimeron and katepisternum except bare part between the top and the bottom covered with long white pile; proepisternum and proepimeron covered with medium white pile; katatergite with short light yellowish pile; anterior anepisternum, katepimeron, meron and metapleuron bare). Legs black, paratype No. 2 with partly dark brownish-black legs, covered with pale pile, long pile whitish, short pile sometimes yellowish-white on tarsi. Wing (Fig. 6D). Length 5.2–5.5 mm. Almost hyaline, entirely microtrichose, clearly yellowish basally, as well as veins yellow near base but brownish apically; pterostigma yellow; vein M joining vein R 4+5 at right angle; calypter whitish with yellowish edge; halter stem light greyish-brownish, knob dark. Abdomen (Fig. 6A, C, G): general colour black. Tergite I slightly rugose, tergites II–III distinctly transversely rugose, tergite IV almost unnoticeably rugose. Tergites I–III matt black dorsally with distinct pollinosity between wrinkles, slightly greenish-bronze on their sides, tergite IV shiny bronze except triangular matt black spot on front edge of tergite, tergite VIII shiny black with goldish to greenish-bronze shine. Abdomen covered with moderately long protruding white pile, longest ones located on the tergite sides, shortest ones located on tergite dorsally. Sternites shiny, greenish-bronze with inconspicuous transverse rugosity, gently pollinose, covered with protruding and adpressed white pile. Genitalia (Fig. 7). Surstyli relatively short and almost rectangular in lateral view, with small hook-like process on apex, but slightly emarginated apically in ventral view (Fig. 7A, B); cerci almost triangular in dorsal view. FEMALE (Fig. 6B, H–J). Body length: 6.0– 6.5 mm. Similar to the male except for sexual dimorphism, and differing by somewhat smaller body size, shorter body pilosity and the following characters. Head (Fig. 6 H–J). Frons, vertex and occiput shining, with sparse short white pile; transverse furrows of the frons absent; frons and face broad (ratio of the maximum head width to the frons width at the level of the antennal base 1.7–1.8, ratio of the maximum head width at the level of the antennal base to the vertex width 2.6–2.7). Face pollinose in upper half, shiny in lower half; facial pile whitish; scape and pedicel black; basoflagellomere brownish-black; arista black. Thorax (Fig. 6B). Scutum and scutellum shiny black with weak bluish shine (2 paratypes) or without such shine (1 paratype), covered with short whitish pile; legs black. Wing (Fig. 6B). Length 5.5–5.8 mm, yellowish. Abdomen (Fig. 6B). Tergite IV not rugose, black, without matt black triangular spot on anterior edge of tergite, tergite V black. Abdomen covered with short protruding white pile. Sternites shiny, brownish black, finely punctated, covered with subadpressed pale pile of equal length. Etymology. The specific name refers to the common nocturnal mammal (Procyon lotor (Linnaeus, 1758), “raccoon” in English and in Ukrainian) native to North America and introduced in the Palaearctic Region, with whom the new species shares such characteristics as the contrasting black-and-white colouration and the ability to climb high up. Distribution. Central Afghanistan (Fig. 8). Biology. Melanogaster raccoon sp. nov. occurs at altitudes of 2400–3200 m above sea level. The species inhabits in mesophilous valleys between dry semi-desert areas high in the mountains (Fig. 9). Three specimens of M. raccoon sp. nov. were collected from the Band-e Amir National Park and its vicinity about 60 km west of the town of Bamyan in Central Afghanistan. The vegetation of the shores of Lake Band-e Amir shores is peculiar: it is a natural oasis at an altitude of 3000–3050 m along the chain of lakes in the canyon. The surrounding area is a hilly plateau dissected by deep canyons with streams that drain into the Band-e-Amir River, covered with alpine friganoid and tragakantoid steppe. The adults fly from May to June (25.V–30.VI by label data). Nothing is known about the life history of this species, but we can speculate that the larvae are hydrobionts similar to the known larvae of other species belonging in this genus (Hennig, 1952; Hartley, 1961; Maibach & Goeldlin, 1994).Published as part of Popov, Grigory V. & Prokhorov, Alexey V., 2020, Revision of the Melanogaster jaroslavensis group (Diptera: Syrphidae), with description of a new species from Afghanistan, pp. 536-552 in Zootaxa 4743 (4) on pages 545-547, DOI: 10.11646/zootaxa.4743.4.4, http://zenodo.org/record/369061
Polarimetrinen ja spektrinen kuvantamismenetelmä epähomogeenisten sirontaväliaineiden, mukaan lukien biologisen kudoksen, kvantitatiiviseen kuvaamiseen
No full textAbstractIn this thesis, light-tissue interaction was investigated through different experimental and analytical methodologies to analyze optical and polarization properties over time and the obtained results were utilized in biomedical and food science applications. In this thesis, soft biological tissues (pork samples) were selected as a biological tissue due to their similarity to human tissue. First, the relative spectral changes of absorbance were studied by applying two different custom-built configurations. A Monte Carlo modelling and principal component analysis (PCA) method were applied further to the absorbance dataset to provide thorough studies for a spectroscopic approach. Second, a novel application of Mueller matrix (MM) imaging polarimetry was pioneered to visualize the dynamics of the tissue polarization properties over time with a custom-built Mueller matrix imaging polarimeter (MMIP). Frequency distribution histograms (FDHs) and the changes in the statistical moments of the MM elements were analyzed over time to provide qualitative and quantitative information of the tissue polarization properties. Finally, a new Stokes-polarimetry was introduced to examine optically thin histological sections from optically anisotropic biological tissues with different morphological structures.In summary, in spectroscopic and imaging polarimetry approaches, prominent changes in optical properties of the examined soft biological tissues were discriminated over time. The obtained results are promising in the development of a novel non-destructive tool for monitoring biological tissues for application in biomedical applications and the food industry. The Stokes-polarimetry method can provide a comparative analysis of different polarimetric techniques and prove the diagnostic potential of Stokes correlometry of pathological changes in the orientation-phase structure of biological tissues.Original papersOriginal papers are not included in the electronic version of the dissertation.Peyvasteh, M., Popov, A., Bykov, A., Pierangelo, A., Novikova, T., & Meglinski, I. (2020). Evolution of raw meat polarization‐based properties by means of Mueller matrix imaging. Journal of Biophotonics, 14(5). https://doi.org/10.1002/jbio.202000376Self-archived versionPeyvasteh, M., Popov, A., Bykov, A., & Meglinski, I. (2020). Meat freshness revealed by visible to near-infrared spectroscopy and principal component analysis. Journal of Physics Communications, 4(9), 095011. https://doi.org/10.1088/2399-6528/abb322Self-archived versionPeyvasteh, M., Dubolazov, A., Popov, A., Ushenko, A., Ushenko, Y., & Meglinski, I. (2020). Two-point Stokes vector diagnostic approach for characterization of optically anisotropic biological tissues. Journal of Physics D: Applied Physics, 53(39), 395401. https://doi.org/10.1088/1361-6463/ab9571Self-archived versionBorovkova, M., Peyvasteh, M., Dubolazov, O., Ushenko, Y., Ushenko, V., Bykov, A., Deby, S., Rehbinder, J., Novikova, T., & Meglinski, I. (2018). Complementary analysis of Mueller-matrix images of optically anisotropic highly scattering biological tissues. Journal of the European Optical Society-Rapid Publications, 14(1). https://doi.org/10.1186/s41476-018-0085-9Self-archived versionPeyvasteh, M., Popov, A., Bykov, A., & Meglinski, I. V. (2019). Assessment of pork freshness based on changes in constituting chromophores using visible to near-infrared spectroscopy. In E. M. Goldys & B. C. Gibson (Eds.), Biophotonics Australasia 2019. SPIE. https://doi.org/10.1117/12.2539969Self-archived versionPeyvasteh, M., Popov, A., Bykov, A., & Meglinski, I. (2019). Assessment of meat freshness and spoilage detection utilizing visible to near-infrared spectroscopy. In A. Amelink & S. K. Nadkarni (Eds.), Novel Biophotonics Techniques and Applications V. SPIE. https://doi.org/10.1117/12.2527194Self-archived versionTiivistelmäTässä opinnäytetyössä valon ja kudoksen vuorovaikutusta tutkittiin erilaisilla kokeellisilla ja analyyttisillä menetelmillä, jotta voitiin tunnistaa optisten ja polarisaatio-ominaisuuksien alkuvaiheita ja hyödyntää saatuja tuloksia biolääketieteellisissä ja elintarviketieteellisissä sovelluksissa. Tässä työssä pehmeäksi biologiseksi kudokseksi valittiin sianlihanäytteet niiden ja ihmiskudoksen samankaltaisuuden vuoksi. Ensiksi pehmeiden biologisten kudosten absorbanssin suhteellisia spektrimuutoksia tutkittiin soveltamalla kahta erilaista räätälöityä kokoonpanoa, jotka yhdistettiin. Monte Carlon mallintamista ja pääkomponenttianalyysimenetelmää (PCA) sovellettiin edelleen absorbanssiaineistoon, jota voitiin perusteellisesti tutkia spektroskooppisesti. Toiseksi Mueller-matriisin (MM) kuvantamispolarimetrian uudella sovelluksella pyrittiin visualisoimaan kudosten polarisaatio-ominaisuuksien dynamiikkaa ajan suhteen räätälöidyllä Mueller-matriisikuvauspolarimetrillä (MMIP). Taajuusjakauman histogrammit (FDH) ja MM-elementtien tilastolliset momenttimuutokset analysoitiin ajan suhteen, jotta saataisiin kvalitatiivista ja kvantitatiivista tietoa kudosten polarisaatio-ominaisuuksista. Lopuksi otettiin käyttöön uusi Stokesin polarimetrinen menetelmä, jotta voitiin tutkia optisesti ohuita histologisia leikkauksia anisotrooppisista biologisista kudoksista, joilla on erilainen morfologinen rakenne.Yhteenvetona voidaan todeta, että spektroskooppisesti ja kuvantamispolarimetrillä tutkittujen pehmeiden biologisten kudosten optisten ominaisuuksien huomattavat muutokset erotettiin selvästi ajan suhteen. Saadut tulokset ovat lupaavia, kun kehitetään uutta ainetta rikkomatonta työkalua biologisten kudosten seurantaan biolääketieteellisissä sovelluksissa ja elintarviketeollisuudessa. Stokesin polarimetrisella menetelmällä voidaan tuottaa vertaileva analyysi erilaisista polarimetrisistä tekniikoista ja todistaa Stokesin korrelometrian diagnostinen potentiaali biologisten kudosten orientaatiovaiheen rakenteessa.OsajulkaisutOsajulkaisut eivät sisälly väitöskirjan elektroniseen versioon.Peyvasteh, M., Popov, A., Bykov, A., Pierangelo, A., Novikova, T., & Meglinski, I. (2020). Evolution of raw meat polarization‐based properties by means of Mueller matrix imaging. Journal of Biophotonics, 14(5). https://doi.org/10.1002/jbio.202000376Rinnakkaistallennettu versioPeyvasteh, M., Popov, A., Bykov, A., & Meglinski, I. (2020). Meat freshness revealed by visible to near-infrared spectroscopy and principal component analysis. Journal of Physics Communications, 4(9), 095011. https://doi.org/10.1088/2399-6528/abb322Rinnakkaistallennettu versioPeyvasteh, M., Dubolazov, A., Popov, A., Ushenko, A., Ushenko, Y., & Meglinski, I. (2020). Two-point Stokes vector diagnostic approach for characterization of optically anisotropic biological tissues. Journal of Physics D: Applied Physics, 53(39), 395401. https://doi.org/10.1088/1361-6463/ab9571Rinnakkaistallennettu versioBorovkova, M., Peyvasteh, M., Dubolazov, O., Ushenko, Y., Ushenko, V., Bykov, A., Deby, S., Rehbinder, J., Novikova, T., & Meglinski, I. (2018). Complementary analysis of Mueller-matrix images of optically anisotropic highly scattering biological tissues. Journal of the European Optical Society-Rapid Publications, 14(1). https://doi.org/10.1186/s41476-018-0085-9Rinnakkaistallennettu versioPeyvasteh, M., Popov, A., Bykov, A., & Meglinski, I. V. (2019). Assessment of pork freshness based on changes in constituting chromophores using visible to near-infrared spectroscopy. In E. M. Goldys & B. C. Gibson (Eds.), Biophotonics Australasia 2019. SPIE. https://doi.org/10.1117/12.2539969Rinnakkaistallennettu versioPeyvasteh, M., Popov, A., Bykov, A., & Meglinski, I. (2019). Assessment of meat freshness and spoilage detection utilizing visible to near-infrared spectroscopy. In A. Amelink & S. K. Nadkarni (Eds.), Novel Biophotonics Techniques and Applications V. SPIE. https://doi.org/10.1117/12.2527194Rinnakkaistallennettu versioAcademic dissertation to be presented with the assent of the Doctoral Training Committee of Information Technology and Electrical Engineering of the University of Oulu for public defence in the Saalasti Hall, Linnanmaa, on 30 September 2021, at 12 noonAbstract
In this thesis, light-tissue interaction was investigated through different experimental and analytical methodologies to analyze optical and polarization properties over time and the obtained results were utilized in biomedical and food science applications. In this thesis, soft biological tissues (pork samples) were selected as a biological tissue due to their similarity to human tissue. First, the relative spectral changes of absorbance were studied by applying two different custom-built configurations. A Monte Carlo modelling and principal component analysis (PCA) method were applied further to the absorbance dataset to provide thorough studies for a spectroscopic approach. Second, a novel application of Mueller matrix (MM) imaging polarimetry was pioneered to visualize the dynamics of the tissue polarization properties over time with a custom-built Mueller matrix imaging polarimeter (MMIP). Frequency distribution histograms (FDHs) and the changes in the statistical moments of the MM elements were analyzed over time to provide qualitative and quantitative information of the tissue polarization properties. Finally, a new Stokes-polarimetry was introduced to examine optically thin histological sections from optically anisotropic biological tissues with different morphological structures.
In summary, in spectroscopic and imaging polarimetry approaches, prominent changes in optical properties of the examined soft biological tissues were discriminated over time. The obtained results are promising in the development of a novel non-destructive tool for monitoring biological tissues for application in biomedical applications and the food industry. The Stokes-polarimetry method can provide a comparative analysis of different polarimetric techniques and prove the diagnostic potential of Stokes correlometry of pathological changes in the orientation-phase structure of biological tissues.Tiivistelmä
Tässä opinnäytetyössä valon ja kudoksen vuorovaikutusta tutkittiin erilaisilla kokeellisilla ja analyyttisillä menetelmillä, jotta voitiin tunnistaa optisten ja polarisaatio-ominaisuuksien alkuvaiheita ja hyödyntää saatuja tuloksia biolääketieteellisissä ja elintarviketieteellisissä sovelluksissa. Tässä työssä pehmeäksi biologiseksi kudokseksi valittiin sianlihanäytteet niiden ja ihmiskudoksen samankaltaisuuden vuoksi. Ensiksi pehmeiden biologisten kudosten absorbanssin suhteellisia spektrimuutoksia tutkittiin soveltamalla kahta erilaista räätälöityä kokoonpanoa, jotka yhdistettiin. Monte Carlon mallintamista ja pääkomponenttianalyysimenetelmää (PCA) sovellettiin edelleen absorbanssiaineistoon, jota voitiin perusteellisesti tutkia spektroskooppisesti. Toiseksi Mueller-matriisin (MM) kuvantamispolarimetrian uudella sovelluksella pyrittiin visualisoimaan kudosten polarisaatio-ominaisuuksien dynamiikkaa ajan suhteen räätälöidyllä Mueller-matriisikuvauspolarimetrillä (MMIP). Taajuusjakauman histogrammit (FDH) ja MM-elementtien tilastolliset momenttimuutokset analysoitiin ajan suhteen, jotta saataisiin kvalitatiivista ja kvantitatiivista tietoa kudosten polarisaatio-ominaisuuksista. Lopuksi otettiin käyttöön uusi Stokesin polarimetrinen menetelmä, jotta voitiin tutkia optisesti ohuita histologisia leikkauksia anisotrooppisista biologisista kudoksista, joilla on erilainen morfologinen rakenne.
Yhteenvetona voidaan todeta, että spektroskooppisesti ja kuvantamispolarimetrillä tutkittujen pehmeiden biologisten kudosten optisten ominaisuuksien huomattavat muutokset erotettiin selvästi ajan suhteen. Saadut tulokset ovat lupaavia, kun kehitetään uutta ainetta rikkomatonta työkalua biologisten kudosten seurantaan biolääketieteellisissä sovelluksissa ja elintarviketeollisuudessa. Stokesin polarimetrisella menetelmällä voidaan tuottaa vertaileva analyysi erilaisista polarimetrisistä tekniikoista ja todistaa Stokesin korrelometrian diagnostinen potentiaali biologisten kudosten orientaatiovaiheen rakenteessa
Popov-Type Criterion for Consensus in Nonlinearly Coupled Networks
Get PDFThis paper addresses consensus problems in nonlinearly coupled networks of dynamic agents described by a common and arbitrary linear model. Interagent interaction rules are uncertain but satisfy the standard sector condition with known sector bounds; both the agent's model and interaction topology are time-invariant. A novel frequency-domain criterion for consensus is offered that is similar to and extends the classical Popov's absolute stability criterion
Acoustic scattering from an infinitely long cylindrical shell with an internal mass attached by multiple axisymmetrically distributed stiffeners
No full textA thin infinitely long elastic shell is stiffened by J in number identical lengthwise ribs distributed uniformly around the circumference and joined to a rod in the center. The 2D model of the substructure is a rigid central mass supported by J axisymmetrically placed linear springs. The response of the shell-spring-mass system is quite different from a fluid filled shell or that of a solid cylinder due to the discrete number of contact points which couple the displacement of the shell at different locations. Exterior acoustic scattering due to normal plane wave incidence is solved in closed form for arbitrary J. The scattering matrix associated with the normal mode solution displays a simple structure, composed of distinct sub-matrices which decouple the incident and scattered fields into J families. The presence of a springs-mass substructure causes resonances which are shown to be related to the subsonic shell flexural waves, and an approximate analytic expression is derived for the quasi-flexural resonance frequencies. Numerical simulations indicate that the new solution for three or more springs results in a complicated scattering response for plane wave incidence. As the number of springs becomes large enough, the total scattering cross-section is asymptotically zero at low frequencies and slightly increased compared to the empty shell at moderate frequencies due to the added stiffness and mass. It is also observed that the sensitivity to the angle of incidence diminishes as the number of springs is increased. This system can be tuned by selecting the shell thickness, spring stiffness and added mass to yield desired quasi-static effective properties making it a candidate element for graded index sonic crystals
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