52 research outputs found
Identification of novel interacting partner of granulin-epithelin precursor in hepatocellular carcinoma
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Dataset of the Botrytis cinerea phosphoproteome induced by different plant-based elicitors
No full textAbstractPhosphorylation is one of the main post-translational modification (PTM) involved in signaling network in the ascomycete Botrytis cinerea, one of the most relevant phytopathogenic fungus. The data presented in this article provided a differential mass spectrometry-based analysis of the phosphoproteome of B. cinerea under two different phenotypical conditions induced by the use of two different elicitors: glucose and deproteinized Tomate Cell Walls (TCW). A total 1138 and 733 phosphoproteins were identified for glucose and TCW culture conditions respectively. Raw data are deposited at the ProteomeXchange Consortium via the PRIDE partner repository with the data set identifier (PRIDE: http://www.ebi.ac.uk/pride/archive/projects/PXD003099). Further interpretation and discussion of these data are provided in our research article entitled “Phosphoproteome analysis of B.cinerea in response to different plant-based elicitors” (Liñeiro et al., 2016) [1]
Stabilization of high-valent transition-metal complexes with macrocyclic tertiary amines. Reinvestigation of the synthesis, electrochemistry, and spectroscopy of osmium (III) macrocyclic amine complexes and X-ray crystal structure of trans-[OsIII(16-TMC)Cl2]ClO4 (16-TMC = 1,5,9,13-Tetramethyl-1,5,9,13-tetraazacyclohexadecane)
No full textDropwise addition of an ethanolic solution of Na2OsCl6 to a refluxing ethanolic suspension of L and tin plates yielded trans-[OsIIILCl2]+ [L = 14aneN4 (1,4,8,11-tetraazacyclotetradecane), 15aneN4 (1,4,8,12-tetraazacyclopentadecane), 16aneN4 (1,5,9,13-tetraazacyclohexadecane), teta (meso-5,5,7,12,12,14-hexamethyl-1,4,8,11-tetraazacyclotetradecane), 16-TMC (1,5,9,13-tetramethyl-1,5,9,13-tetraazacyclohexadecane)]. The UV-vis spectra of trans-[OsIIILCl2]+ exhibited two ligand-to-metal charge-transfer (LMCT) pπ(Cl) → dπ(Os) transition bands in the 250-280- and 280-310-nm regions. The LMCT transition energy for trans-[OsIIILCl2]+ varies with L = (NH3)4 > (en)2 > 2,3,2-tet > 3,2,3-tet > 14aneN4 > 15aneN4 ∼ 16aneN4 > 16-TMC > teta (2,3,2-tet = 3,7-diazanonane-1,9-diamine; 3,2,3-tet = 4,7-diazadecane-1,10-diamine). In acetonitrile, reversible Os-(III)/Os(II) couples were observed at potentials -1.6 to -1.3 V vs. the Cp2Fe+/0 couple. The E1/2 value for trans-[OsIIILCl2]+ is relatively insensitive to the macrocyclic ring size and decreases from L = 14aneN4 to L = teta. For Os(III) secondary amine complexes, the electrochemical oxidation of Os(III) to Os(IV) was irreversible. For trans-[OsIII(16-TMC)Cl2]+, a reversible Os(IV)/Os(III) couple was found at 0.67 V vs. the Cp2Fe+/0 couple and 0.81 V vs. NHE in acetonitrile and 0.1 M HCl, respectively. trans-[OsIV(16-TMC)Cl2]2+ has been characterized spectroscopically with a pπ(Cl) → dπ[Os(IV)] transition at 365 nm. The structure of trans-[OsIII(16-TMC)Cl2]ClO4 has been determined by X-ray crystallography: orthorhombic; space group Pnma; a= 14.982(3), b = 11.036(3), c= 13.586 (4) Å; V= 2246.3 (9) Å3; Z = 4. The N-methyl groups of the 16-TMC ligand adopt a "two up" and "two down" configuration, and the two chloride ligands are trans to each other. The Os-N and Os-Cl distances are 2.263 (17) and 2.348 (1) Å, respectively. © 1987 American Chemical Society.link_to_subscribed_fulltex
Solid-state emission of dicyanoplatinum(II) and -palladium(II) complexes of substituted 2,2′-bipyridines and 1,10-phenantliroline and X-ray crystal structures of isomorphous M(bpy) (CN)2 (bpy = 2,2′-bipyridine; M = Pt, Pd)
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Preparation of thin dielectric film for nonvolatile memory by thermal oxidation of Si-rich LPCVD nitride
No full textIn this work, we develop methods for fabricating high quality dielectric films for nonvolatile memory applications. Oxide/Si-rich nitride/oxide structures are fabricated where the Si-rich nitride layer was deposited by the low pressure chemical vapor deposition (LPCVD) technique. With a Si-rich nitride layer, the Fowler-Nordheim tunneling voltage can be cut down to 3 V for oxide thickness of about 100 Angstrom. By reoxidizing the Si-rich nitride layer, secondary ion mass spectroscopy study reveals that the hydrogen content of nitride film and its interface can be reduced by more than 40\%. With this method, high nitrogen content oxynitride and smoother oxynitride/oxide interfaces result and the interface charge trapping can be improved remarkably. (C) 2001 The Electrochemical Society
SIMS study of silicon oxynitride prepared by oxidation of silicon-rich silicon nitride layer
No full textWe proposed a novel process for fabrication silicon oxide-oxynitride-oxide structure for ULSI device applications. By deposition of silicon-rich silicon nitride and then following a thermal oxidation process, a good oxynitride layer was obtained. Secondary ion mass spectroscopy (SIMS) study reveals that the hydrogen content of nitride film at the interface can be reduced by more than 40\% when compared to stoichiometric nitride. With this method, high nitrogen content oxynitride and smoother oxynitride/oxide interfaces result in the reduction of the interface charge trapping remarkably. (C) 2001 Elsevier Science Ltd. All rights reserved
Cis ruthenium complexes of 1,4,8,11-tetraazacyclotetradecane (cyclam): Crystal and molecular structure of cis-[Ru(cyclam)Cl2]Cl
No full textThe crystal and molecular structure of cis-[Ru(cyclam)Cl2]Cl (cyclam = C10H24N4) prepared by the Ru(ox)3 3- (ox = C2O4 2-) method has been determined: monoclinic, space group P21/c, a = 7.579 (1) Å, b = 14.414 (4) Å, c = 14.718 (3) Å, β = 102.33 (1)°, V = 1570.8 (5) Å3, Z = 4. The average Ru-Cl distance, 2.371 (1) Å in the present complex, is substantially longer than that found in the corresponding trans isomer [2.342 (1) Å], reflecting the greater σ-trans effect of the secondary amine N atom over the chloride ligand. Reaction of cis-[Ru(cyclam)Cl2]Cl with N heterocyclic amines and Zn(Hg) yielded cis-[Ru(cyclam)L2]2+ (L2 = (py)2, bpy, phen), the metal-to-ligand charge-transfer transitions of which have been found to be blue shifted from those of the corresponding cis-[Ru(NH3)4L2]2+ species. © 1985 American Chemical Society.link_to_subscribed_fulltex
Interacting protein of granulin-epithelin precursor in liver cancer
No full textBACKGROUND AND OBJECTIVE: Granulin-epithelin precursor (GEP), a novel growth factor, has been identified as a potential therapeutic target from our earlier genome-wide expression profile study on liver cancer. GEP over-expressed in liver cancer, and regulated proliferation, invasion and tumorigenesis. We demonstrated the therapeutic approach of GEP targeted therapy in human cancer using the home-made GEP monoclonal antibody on animal model, and GEP antibody showed dose-dependent inhibitory effect on tumor growth. However, the exact mechanism on how GEP executes its biological function, and in particular its interacting protein partners, is largely unknown. The present study aims to identify and characterize the GEP interacting protein ...link_to_OA_fulltex
Traffic tolerance of fine fescues: techniques for screening germplasm
No full textThe term fine fescue refers to several Festuca spp. that have a very fine leaf texture compared to most other turfgrass species. These species are adapted to low-input management systems and have been used in mixtures with other cool-season grasses. However, fine fescues are not utilized to the same extent as other species partially due to their poor traffic tolerance and recuperative ability. Improvement in traffic tolerance of fine fescues would enable use of these grasses beyond turf systems that experience little to no traffic.
The purpose of this dissertation was to develop and evaluate germplasm screening techniques that improve selecting efficiency for traffic tolerant fine fescues. The specific objectives of this research were: i) to evaluate the effect of traffic form (abrasive wear vs. cleated traffic) and season (spring vs. summer vs. autumn) on the assessment of fine fescue traffic tolerance (Chapters 1 and 2); ii) to evaluate the effect of nitrogen fertilization and harvest time on cell wall composition of fine fescues (Chapters 3 and 4); iii) to investigate the correlation between cell wall composition and wear tolerance of fine fescues; and iv) to develop near-infrared reflectance spectroscopy (NIRS) models to determine the cell wall composition of fine fescues.
For the first objective, the ability of fine fescue turf to maintain a dense cover depended on the specific traffic form and varied based on the season during which wear stress occurred. Abrasive wear, applied with Rutgers Wear Simulator, caused more thinning of the turf canopy than cleated traffic applied with the Cady Traffic Simulator. Thus, abrasive wear resulted in a greater separation among fine fescues based on the fullness of turf cover (FTC) and will likely improve selection efficiency compared to cleated traffic. The FTC response of fine fescues were more sensitive to traffic stress during summer because of the high disease pressure and heat stress. Screening for fine fescues for improved traffic tolerance during spring would probably be less biased and more effective at identifying tolerance to traffic within fine fescues because other abiotic or biotic stresses would be avoided or minimized. Abrasive wear was also effective in identifying cultivars that are susceptible to leaf bruising. Leaf bruising was more severe during summer and autumn due to the heat stress. However, leaf bruising response of fine fescue cultivars varied with the season; sheep fescue and strong creeping red fescue were more susceptible to leaf bruising during summer while Chewings fescue and slender creeping red fescue were more bruised during autumn. Thus, evaluation of this characteristic would need to be conducted in both the summer and autumn.
For the second objective, N fertilization was expected to alter cell wall composition as it promotes new leafy growth, which would be expected to have lower cell wall content. However, the effect of N fertilization on cell wall composition was not observed. Differences among three fine fescue species were significant and the ranking of total cell wall (TCW) content remained the same throughout the entire study: hard fescue > Chewings fescue > strong creeping red fescue. Harvest time (season) had a significant impact on cell wall composition; TCW content was greater in summer (August) compared to spring (May) and autumn (November). However, the relative ranking among five fine fescue species was consistent despite the fluctuation in concentration caused by the harvest time. The concentrations of TCW, hemicellulose, lignocellulose and cellulose from high to low were: Sheep fescue > hard fescue > Chewings fescue = slender creeping red fescue > strong creeping red fescue.
For the third objective, a general pattern between wear tolerance and verdure biomass and cell wall constituents of fine fescues were identified on an inter-specific level; improved wear tolerance was associated with greater verdure biomass, TCW, and lignocellulose content as well as reduced lignin content. Correlations were not as strong on an intra-specific level which is probably due, at least in part, to a narrower range of diversity for these characteristics within each species. The potential to improve wear tolerance by selecting for greater verdure biomass, TCW and lignocellulose contents appeared to be more promising for Chewings and strong creeping red fescues than hard fescue.
For the fourth objective, NIRS provided a relatively rapid and precise method of estimating total N (R2cal > 0.93) in the verdure at both the inter- and intra-specific levels. Due to the complexity of the constituents, the prediction accuracy was less favorable, but reliable, for TCW (R2cal > 0.70), lignocellulose (R2cal > 0.78) and hemicellulose (R2cal > 0.79) at the intra or inter specific level(s). Further development of these NIRS prediction models would facilitate future research that defines the correlation between cell constituents, such as total N and cell wall composition, and the traffic tolerance of fine fescues.
This research advanced our understanding of methods to assess the traffic tolerance of fine fescues. Screening efficacy and accuracy can be improved using abrasive wear during spring when confounding stresses are minimal. This research indicated traffic tolerance among fine fescue species is positively associated with verdure biomass and cell wall content. It is also possible to select traffic tolerant cultivars within each species based on these traits with considerable phenotypic variability in wear tolerance. The development of NIRS calibration models will facilitate future breeding applications to rapidly identify fine fescue cultivars with greater cell wall content. The findings from this research will contribute to the sustainability of the turfgrass industry by improving screening techniques for developing traffic tolerant fine fescues cultivars for use on low-input landscapes.Ph.D.Includes bibliographical reference
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