36 research outputs found
NGAL and TBARS quantification.
No full text(A) Absolute NGAL (ng) levels in urine and (B) TBARS (MDA in μM) in the perfusate of the three experimental groups during NMP (mean ± SD).</p
Albumin levels.
No full text(A) Albumin concentration (g/l) in the perfusate and (B) albumin concentration in urine (g/l) and (C) AUC of albumin in urine of the three experimental groups during NMP (mean ± SD). *** indicates statistical significance of ≤ 0.001.</p
Histological evaluation of a kidney perfused with human RBCs.
No full text(A) An example of a light microscopy image of HE-stained biopsy of a biopsy of a porcine kidney perfused with human RBC-based solution after t = 420 min of NMP; (B) Focal lesion around a blood vessel in a HE-stained biopsy of a porcine kidney perfused with a human RBC-based solution after 420 minutes of NMP.</p
Markers of general cell injury.
No full text(A) ASAT levels in perfusate (U/l) and (B) AUC of the three experimental groups during NMP (mean ± SD). (C) LDH levels in perfusate (U/l) and (D) AUC of the three experimental groups during NMP (mean ± SD). * indicates statistical significance of ≤ 0.05. ** indicates statistical significance of ≤ 0.01.</p
Normothermic machine perfusion of ischaemically damaged porcine kidneys with autologous, allogeneic porcine and human red blood cells
Full text linkIn porcine kidney auto-transplant models, red blood cells (RBCs) are required for ex-vivo normothermic machine perfusion (NMP). As large quantities of RBCs are needed for NMP, utilising autologous RBCs would imply lethal exsanguination of the pig that is donor and recipient-to-be in the same experiment. The purpose of this study was to determine if an isolated porcine kidney can also be perfused with allogeneic porcine or human RBCs instead. Porcine kidneys, autologous and allogeneic blood were obtained from a local slaughterhouse. Human RBCs (O-pos), were provided by our transfusion laboratory. Warm ischaemia time was standardised at 20 minutes and subsequent hypothermic machine perfusion lasted 1.5–2.5 hours. Next, kidneys underwent NMP at 37°C during 7 hours with Williams' Medium E and washed, leukocyte depleted RBCs of either autologous, allogeneic, or human origin (n = 5 per group). During perfusion all kidneys were functional and produced urine. No macroscopic adverse reactions were observed. Creatinine clearance during NMP was significantly higher in the human RBC group in comparison with the allogeneic group (P = 0.049) but not compared to the autologous group. The concentration of albumin in the urine was significantly higher in the human RBC group (P </div
Individual data per kidney per group at t = 60, t = 180, t = 300 and t = 420.
No full textIndividual data per kidney per group at t = 60, t = 180, t = 300 and t = 420.</p
Mean flow per 100 g of the three experimental groups during NMP (± SD).
No full text* indicates statistical significance of ≤ 0.05.</p
Mean cumulative diuresis (ml) of the three experimental groups during NMP (± SD).
No full text* indicates statistical significance of ≤ 0.05.</p
Histological evaluation if a kidney perfused with allogeneic RBCs.
No full textAn example of a light microscopy image of HE-stained biopsy of a biopsy of a porcine kidney perfused with allogeneic RBC-based solution after t = 420 min of NMP.</p
Histological damage scoring of cortical biopsies taken at t = -10, t = 240 and t = 420 (mean ± SD).
No full textHistological damage scoring of cortical biopsies taken at t = -10, t = 240 and t = 420 (mean ± SD).</p
