1,362,144 research outputs found
Chemical Secretory Pathway Modulation in Plant Protoplasts
No full textthe classical Golgi pathway is not the only mechanism for vacuolar protein transport in plants because alternative transport mechanisms have been described. The existence of these alternative pathways can be demonstrated using several chemicals and here we describe the use of brefeldin A (BFA), endo-β-N-acetylglucosaminidase H (Endo-H), and tunicamycin, on isolated tobacco leaf protoplasts. Two main methods are illustrated in this chapter, protoplast pulse-chase followed by protein immunoprecipitation, and protoplast immunofluorescence
The Beauty of Inclusivity: “Visual Activism” from Social Media to Fashion Magazines
No full textThis article investigates the role of fashion and beauty photography in
contemporary social discourses of inclusion and diversity, contributing to a critique of traditional politics of representation of the body and, therefore, to sociocultural change. Starting from analyzing this complex phenomenon that has generated a ground for comparison between ethic and aesthetic practices, snapshot culture, and advertising, Pompa examines how the rise of social media put into crisis the now leading aesthetic standards. In fact, these media have pointed out the need to increase the representation on fashion magazines of minorities, who have until now been excluded in the mainstream media. In this perspective, Vogue Italia and related narratives of unconventional beauty are explored
Borbély Szilárd Halotti Pompa című kötetének elemzése
No full textSzakdolgozatom témája Borbély Szilárd Halotti Pompa című verseskötetének elemzése bizonyos szempontok alapján. A címből adódóan fontosnak találtam a kötetben egymás mellett előforduló barokk és posztmodern jegyek vizsgálatát és értelmezését. Borbély Szilárd életrajzát ismerve el nem hanyagolható szempont még az autoreferencialitás kérdésének vizsgálata is, melyhez az Egy gyilkosság mellékszálai című interjúgyűjteményt vettem alapul. Igyekeztem párhuzamosan a kötet makro- és mikroelemzését is elvégezni: az egyes versek, a kötetet kitöltő három ciklus, illetve a kötet egészként való értelmezése egyaránt relevánsnak mutatkozott számos elemzési szempontot figyelembe véve. A Testhez című kötetet – amelyet a Halotti Pompa folytatásának tartanak – is említem a dolgozatomban, valamint összehasonlítom a két kötet szervezőelvét, ábrázolásmódját.egységes, osztatlanmagyar nyelv és irodalom tanár, angoltaná
Mechanism of protein body formation, one of the routes of protein storage in plant cells
No full textRuolo del Reticolo Endoplasmatico nella Sintesi, nel Traffico e nell’Accumulo di Proteine
No full text”An autoregulatory mechanism modulates translation of heterologous proteins in tobacco chloroplast”
No full textRetention of a Bean Phaseolin/Maize γ-Zein Fusion in the Endoplasmic Reticulum Depends on Disulfide Bond Formation
No full textMost seed storage proteins of the prolamin class accumulate in the endoplasmic reticulum (ER) as large insoluble polymers termed protein bodies (PBs), through mechanisms that are still poorly understood. We previously showed that a fusion between the Phaseolus vulgaris vacuolar storage protein phaseolin and the N-terminal half of the Zea mays prolamin γ-zein forms ER-located PBs. Zeolin has 6 Cys residues and, like γ-zein with 15 residues, is insoluble unless reduced. The contribution of disulfide bonds to zeolin destiny was determined by studying in vivo the effects of 2-mercaptoethanol (2-ME) and by zeolin mutagenesis. We show that in tobacco (Nicotiana tabacum) protoplasts, 2-ME enhances interactions of newly synthesized proteins with the ER chaperone BiP and inhibits the secretory traffic of soluble proteins with or without disulfide bonds. In spite of this general inhibition, 2-ME enhances the solubility of zeolin and relieves its retention in the ER, resulting in increased zeolin traffic. Consistently, mutated zeolin unable to form disulfide bonds is soluble and efficiently enters the secretory traffic without 2-ME treatment. We conclude that disulfide bonds that lead to insolubilization are a determinant for PB-mediated protein accumulation in the E
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