4,583 research outputs found
Comparison of two anaerobic water polo-specific tests with the Wingate test
No full textThe purpose of the current study was to compare 2 water polo–specific tests—the 14 3 25-m swims (SWIM) and the 30-second crossbar jumps (30CJ)—with a laboratory-based test of anaerobic power, the Wingate Anaerobic Test (WAnT). Thirteen elite women’s water polo players (mean 6 SD: age 22.0 6 4.4 years, height 168.7 6 7.9 cm, body mass 65.9 6 6.1 kg, body fat 23.6 6 3.5 %, maximum oxygen uptake 51.4 6 4.5 mlkg21min21) participated in the study. The SWIM involved 14 repeated ‘‘all-out’’ sprints every 30 seconds. Swimming time was recorded, and sprint velocity, mean velocity (Vmean), and the gradient of the linear regression equation (GRADIENT) were calculated. The 30CJ involved repeated in-water water polo jumps and touching the goal crossbar with both hands. The number of touches in 30 seconds was recorded. Additionally, the subjects completed a 30-second WAnT, and mean power (Mp) and fatigue index (FI) were calculated. Kendall tau (t) rank correlation was used to examine for correlation between ranks. Significance level was set at p # 0.05. No significant correlation was found between any of the measures of the WAnT and the 2 sport-specific tests. It was suggested that the WAnT may not be an appropriate evaluation tool for anaerobic power assessment of water polo players, stressing the importance of sport-specific tests
STIL binding to Polo-box 3 of PLK4 regulates centriole duplication
No full textPolo-like kinases (PLK) are eukaryotic regulators of cell cycle progression, mitosis and cytokinesis; PLK4 is a master regulator of centriole duplication. Here, we demonstrate that the SCL/TAL1 interrupting locus (STIL) protein interacts via its coiled-coil region (STIL-CC) with PLK4 in vivo. STIL-CC is the first identified interaction partner of Polo-box 3 (PB3) of PLK4 and also uses a secondary interaction site in the PLK4 L1 region. Structure determination of free PLK4-PB3 and its STIL-CC complex via NMR and crystallography reveals a novel mode of Polo-box-peptide interaction mimicking coiled-coil formation. In vivo analysis of structure-guided STIL mutants reveals distinct binding modes to PLK4-PB3 and L1, as well as interplay of STIL oligomerization with PLK4 binding. We suggest that the STIL-CC/PLK4 interaction mediates PLK4 activation as well as stabilization of centriolar PLK4 and plays a key role in centriole duplication
Polo like kinase 2 tumour suppressor and cancer biomarker: new perspectives on drug sensitivity/resistance in ovarian cancer
No full textThe polo-like kinase PLK2 has recently been identified as a potential theranostic marker in the management of chemotherapy sensitive cancers. The methylation status of the PLK2 CpG island varies with sensitivity to paclitaxel and platinum in ovarian cancer cell lines. Importantly, extrapolation of these in vitro data to the clinical setting confirms that the methylation status of the PLK2 CpG island predicts outcomes in patients treated with carboplatin and paclitaxel chemotherapy. A second cell cycle regulator, p57Kip2, is also subject to epigenetic silencing in carboplatin resistance in vitro and in vivo, emphasising that cell cycle regulators are important determinants of sensitivity to chemotherapeutic agents and providing insights into the phenomenon of collateral drug sensitivity in oncology. Understanding the mechanistic basis and identification of robust biomarkers to predict collateral sensitivity may inform optimal use of chemotherapy in patients receiving multiple lines of treatment
Mitotic centromere-associated kinesin (MCAK) : a potential cancer drug target
No full textThe inability to faithfully segregate chromosomes in mitosis results in chromosome instability, a hallmark of solid tumors. Disruption of microtubule dynamics contributes highly to mitotic chromosome instability. The kinesin-13 family is critical in the regulation of microtubule dynamics and the best characterized member of the family, the mitotic centromere-associated kinesin (MCAK), has recently been attracting enormous attention. MCAK regulates microtubule dynamics as a potent depolymerizer of microtubules by removing tubulin subunits from the polymer end. This depolymerizing activity plays pivotal roles in spindle formation, in correcting erroneous attachments of microtubule-kinetochore and in chromosome movement. Thus, the accurate regulation of MCAK is important for ensuring the faithful segregation of chromosomes in mitosis and for safeguarding chromosome stability. In this review we summarize recent data concerning the regulation of MCAK by mitotic kinases, Aurora A/B, Polo-like kinase 1 and cyclin-dependent kinase 1. We propose a molecular model of the regulation of MCAK by these mitotic kinases and relevant phosphatases throughout mitosis. An ever-increasing quantity of data indicates that MCAK is aberrantly regulated in cancer cells. This deregulation is linked to increased malignance, invasiveness, metastasis and drug resistance, most probably due to increased chromosomal instability and remodeling of the microtubule cytoskeleton in cancer cells. Most interestingly, recent observations suggest that MCAK could be a novel molecular target for cancer therapy, as a new cancer antigen or as a mitotic regulator. This collection of new data indicates that MCAK could be a new star in the cancer research sky due to its critical roles in the control of genome stability and the cytoskeleton. Further investigations are required to dissect the fine details of the regulation of MCAK throughout mitosis and its involvements in oncogenesis
META ANALISIS EFFECT SIZE PELATIHAN DRYLAND TERHADAP PENINGKATAN PERFORMA ATLET POLO AIR
No full textPelatihan dryland merupakan salah satu metode dalam meningkatkan kondisi fisik atlet polo air. Untuk mencetak prestasi yang tinggi seorang pelatih dalam program perencanaan latihanya harus yang berkualitas dan berkuantitas serta dilakukan dengan teknik yang baik. Penelitian ini bertujuan untuk mengetahui effect size dari model latihan dryland terhadap peningkatan performa atlet poloair. Metode penelitian menggunakan pendekatan kuantitatif dengan teknik meta analisis. Metode ini menyajikan analisis hasil publikasi penelitian ilmiah pada elektronik jurnal Internasional bereputasi dan jurnal nasional bereputasi yang berkaitan dengan dampak pelatihan dryland terhadap peningkatan performa atlet polo air. Sampel dalam penelitian ini adalah 20 artikel ilmiah yang publish di jurnal internasional terindeks database bereputasi tinggi Scopus kategori Q1/Q2 dengan H-Index, dan artikel yang publish di jurnal internasional dengan homepage Elsevier dengan Impact Factor dari tahun 2011-2020. Instrumen yang digunakan dalam penelitian ini adalah lembar pemberian kode (coding data). Analisis data diperoleh melalui program SPSS versi 21. Hasil uji diperoleh nilai signifikansi atau p 0.000 < 0.05 sehingga model latihan dryland memberikan effect size yang valid dalam meningkatkan performa atlet polo air. Hal penting yang perlu diperhatikan di dalam menerapkan model latihan dryland adalah kategori usia atlet dan intensitas latihan harus sesuai dengan kaidah-kaidah latihan agar memperoleh hasil yang optimal.
Kata Kunci: Dryland, teknik meta analisis, polo air
Dryland training is one method of improving the physical condition of water polo athletes. To score high achievement, a trainer in his training planning program must be of high quality and quantity and be done with good technique. This study aims to determine the effect size of the dryland training model on improving the athlete's performance. The research method uses a quantitative approach with meta-analysis techniques. This method provides an analysis of the results of scientific research publications in reputable international and national journals related to the impact of dryland training on improving the performance of water polo athletes. The samples in this study were 20 scientific articles published in international journals indexed by the highly reputable Scopus database in the Q1 / Q2 category with the H-Index, and articles published in international journals on the Elsevier homepage with Impact Factors from 2011-2020. The instrument used in this study was the coding data sheet. Data analysis was obtained through the SPSS version 21 program. The test results obtained a significance value or p 0.000 <0.05 so that the dryland training model provides a valid effect size in improving the performance of water polo athletes. The important thing that needs to be considered in applying the dryland training model is that the athlete's age category and the training intensity must be in accordance with the training rules in order to obtain optimal results.
Keywords: Dryland, meta analysis techniques, water pol
The SSC of the Generalised Jahangir’s Graph Jm,k and its Algebraic Characterizations
No full textIn this article, we present important combinatorial and algebraicproperties of spanning simplicial complex (SSC) of the generalised Jahangir’sgraph Jm,k. We describe the relation to find f−vectors associatedto Δs(Jm,k) and determine the Hilbert series for the SR-ring KΔs(Jm,k).In the end, we present the associated primes of the facet ideal IF(Δs(Jm,k))and the Cohen-Macaulay characterization of the SR-ring of Δs(Jm,k).AMS (MOS) Subject Classification Codes: Primary 13-P10, Secondary 13-F20, 13-C14, 13-H10.Corresponding Author: Agha KashifKey Words: Simplicial Complexes, f-vectors, Spanning Trees, Face Ring, Hilbert Series, CohenMacaulay
To <i>JM</i> on Its 75th Anniversary
No full text This article discusses how Journal of Marketing ( JM) has influenced marketing science and practice by publishing articles on substantive topics relevant to customers, managers, organizations, markets, and society. The journal's 75th anniversary coincides with the 50th anniversary of the Marketing Science Institute (MSI). Frequently, JM and MSI have collaborated to address important substantive marketing issues identified in MSI's Research Priorities. The author highlights seminal articles on brand equity; business-to-business marketing (including sales force management); connecting marketing information, metrics, and strategy; consumer behavior; innovation, new product development. and product management; marketing orientation and capabilities; and market research, methodology and services. She also draws attention to articles that have won the Sheth Foundation/ JM Award and the H. Paul Root Award. The article describes how JM‘s knowledge dissemination is amplified by powerful social network effects. Ideas in JM articles diffuse through the business community, influencing the mind-set of managers worldwide. </jats:p
Evaluación de la función visual, de la capa de fibras nerviosas de la retina y de la colorimetría de la cabeza del nervio óptico como biomarcadores en pacientes con Alzheimer y fibromialgia
No full textEvaluación de la función visual (agudeza visual, campimetria automatizada, test de color), de los espesores de la capa de fibras nerviosas de la retina medidos mediante 2 dispositivos de OCT (Tomografía de coherencia óptica) y de la colorimetría de la cabeza del nervio óptico (medición mediante una fotografía de la saturación de hemoglobina a través del análisis de color de la misma) como posible uso de los mismos como biomarcadores para el diagnóstico de la Enfermedad de Alzheimer y de la fibromialgia. Demostrándose la utilidad.Artículos empleados para la redacción de la tesis doctoral1. Bambo MP, Garcia-Martin E, Gutierrez-Ruiz F, Pinilla J, Perez-Olivan S, LarrosaJM, Polo V, Pablo L. Analysis of optic disk color changes in Alzheimer's disease: apotential new biomarker. Clin Neurol Neurosurg. 2015 May;132:68-73. doi: 10.1016/j.clineuro.2015.02.016. Epub 2015 Mar 9. PubMed PMID: 25797847.2. Bambo MP, Garcia-Martin E, Gutierrez-Ruiz F, Magallon R, Roca M, Garcia-Campayo J, Perez-Olivan S, Polo V, Larrosa JM, Pablo LE. Study of perfusion changesin the optic disc of patients with fibromyalgia syndrome using new colorimetricanalysis software. J Fr Ophtalmol. 2015 Sep;38(7):580-7. doi: 10.1016/j.jfo.2015.01.010.Epub 2015 May 11. PubMed PMID: 25976129.3. Garcia-Martin E, Garcia-Campayo J, Puebla-Guedea M, Ascaso FJ, Roca M,Gutierrez-Ruiz F, Vilades E, Polo V, Larrosa JM, Pablo LE, Satue M. Fibromyalgia iscorrelated with retinal nerve fiber layer thinning. PLoS One. 2016 Sep;11(9):e0161574.doi: 10.1371/journal.pone.0161574. eCollection 2016. PubMed PMID: 27584145;PubMed Central PMCID: PMC5008644.4. Bambo MP, Garcia-Martin E, Larrosa JM, Polo V, Gutiérrez-Ruiz F, Villages E,Gil-Arribas L, Pablo LE. Retinal and optic disc alterations in Alzheimer’s disease: theeye as a potential central nervous system window. J Alzheimers Dis Parkinsonism2016;6:223. doi: 10.4172/2161-0460.1000223<br /
JM-20, a Benzodiazepine-Dihydropyridine Hybrid Molecule, Inhibits the Formation of Alpha-Synuclein-Aggregated Species
No full text\ua9 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.Studies showed that JM-20, a benzodiazepine-dihydropyridine hybrid molecule, protects against rotenone and 6-hydroxydopamine neurotoxicity. However, its protective effects against cytotoxicity induced by endogenous neurotoxins involved in Parkinson’s disease (PD) pathogenesis have never been investigated. In this study, we evaluated the ability of JM-20 to inhibit alpha-synuclein (aSyn) aggregation. We also evaluated the interactions of JM-20 with aSyn by molecular docking and molecular dynamics and assessed the protective effect of JM-20 against aminochrome cytotoxicity. We demonstrated that JM-20 induced the formation of heterogeneous amyloid fibrils, which were innocuous to primary cultures of mesencephalic cells. Moreover, JM-20 reduced the average size of aSyn positive inclusions in H4 cells transfected with SynT wild-type and synphilin-1-V5, but not in HEK cells transfected with synphilin-1-GFP. In silico studies showed the interaction between JM-20 and the aSyn-binding site. Additionally, we showed that JM-20 protects SH-SY5Y cells against aminochrome cytotoxicity. These results reinforce the potential of JM-20 as a neuroprotective compound for PD and suggest aSyn as a molecular target for JM-20
Efficacy and safety of dorsal penile nerve block before collagenase of clostridium histolyticum injections in peyronie's disease patients: Results from a prospective pilot study
No full textThe intraplaque injection of collagenase from Clostridium Histolyticum (CCH) was established as an effective therapeutic alternative for selected patients with Peyronie's disease (PD). There is no consensus on the use of pre-procedure anaesthesia. The aim of this pilot study was to assess the efficacy and safety of dorsal penile block before CCH injections in reducing procedure related pain. The treatment protocol described in the IMPRESS trials was adopted. The first injection of the first cycle was given without anaesthesia, while the second after penile block. After the administration of each injection, the pain related to the procedure was evaluated with the Wong-Baker-FACES(R)-Pain-Rating-Scale. Thirty patients were included. Mean age 56.7 (SD: 9.61) years. Mean basal penile curvature 59.37o (SD: 18.26). The mean pain value related to the procedure measured after the first injection of the first cycle (without anaesthesia) was 5.4 (SD: 2.13), while after the second injection (with anaesthesia) was 2.5 (SD: 1.92), (p < .001). The treatment was more painful in patients with dorsal plaques (mean:6.2) than in patients with lateral plaques (mean: 4.35) (p = .01). We can conclude that penile block before CCH injection in patients with PD seems an effective and safe measure to decrease the pain related to the procedure
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