87,877 research outputs found
Tony Pizzo\u27s Tampa, Part 10, Coming of Age, 1979
No full textPart Ten, Coming of Age of Tony Pizzo\u27s Tampa Series aired on WUSF in 1979. This episode had guest speaker Jim Walter and focused on Tampa during World War II and beyond. Included are mentions of Drew Field and MacDill Airfield, Dale Mabry Highway, President John F. Kennedy and his visit to Tampa, the Port of Tampa, the decline of Ybor City, the higher education institutions in Tampa (University of Tampa, University of South Florida, and Hillsborough Community College), arts in modern Tampa, Curtis Hixon Hall, and the public library
Marriage record of Pizzo, Giuseppe and Zambito, Emilia
No full textMarriage license for Giuseppe Pizzo and Emilia Zambito. F. Navin was the officiant
Marriage record of Pizzo, Domenico and Giggino, Antonina
No full textMarriage license for Domenico Pizzo and Antonina Guggino. Philip F. Licata was the Notary Public
"Safety health impacts of particulate matter from excavation work sites"
No full textGli studi epidemiologici hanno evidenziato una relazione lineare tra il particolato aerodisperso e gli effetti sulla salute dell'uomo. E' dunque interessante comprendere quale sia realmente il rischio a cui va incontro la popolazione esposta ad importanti sorgenti quali i cantieri di scavo in area urbana, per la costruzione di edifici ed infrastrutture finalizzate alla riqualificazione urbana. La metodologia di valutazione del rischio sanitario, definita dall'OMS nelle linee guida sulla qualità dell'aria per l'Europa, è stata applicata per valutare i possibili effetti sanitari da esposizioni al PM10 per le concentrazioni medie giornaliere ≥ di 50 µg/m3 e ≥ di 100 µg/m3 per tre giorni consecutivi e per aumenti di 10 µg/m3. La presenza del cantiere rileva, indipendentemente dal superamento dei valori limite stabiliti dalla legge, un aumento del rischio sulla salute. Non risulta peraltro individuato un valore di concentrazione di PM10, attribuibile ad una sorgente aggiuntiva (quale in cantiere) sotto il quale sicuramente non ci sia danno. Lo scopo della presente ricerca è dunque quello di stimolare il dibattito e le decisioni delle autorità pubbliche, al fine di approfondire e gestire le problematiche relative al particolato aerodispers
Tethyan magnetostratigrapy from Pizzo Mondello and correlation to the Late Triassic Newark APTS
No full textWe present magnetostratigraphic data and preliminary C13 and O18 stable isotope data from an expanded (≈430m-thick) Upper Triassic marine section at Pizzo Mondello from the Sicani Basin of Sicily and review biostratigraphic data from the literature that can be used to define the location of the Carnian/Norian and Norian/Rhaetian boundaries. Pizzo Mondello offers good potentials for
magnetostratigraphic correlation of marine biostratigraphic and chemostratigraphic data with the continental Newark astrochronological time scale (APTS) for development of an integrated Late Triassic time scale. The relatively stable average values of 18O centered around 0‰ are a strong indication that the Cherty Limestone at Pizzo Mondello suffered very little diagenetic overprinting. The Carnian/Norian boundary at Pizzo Mondello seems to be associated with a positive shift of C13 although further work is necessary to evaluate its paleoenvironmental significance. A statistical approach was applied to evaluate various Pizzo Mondello to Newark magnetostratigraphic correlations. Two correlation options, neither unequivocal, have the highest and nearly equivalent correlation coefficients. Option #1 predicts the base of Pizzo Mondello to be correlative with the middle part of the Newark APTS, whereas in Option #2 the base of Mondello starts towards the early part of the Newark APTS. According to sampling density and average sediment accumulation rates of 20-30m/m.y., polarity intervals with durations equal to or less than ≈170 k.y. may have been undersampled at Pizzo Mondello. Accordingly, we filtered the high resolution Newark APTS and performed further statistical correlations from which we conclude that Option #2 is preferred. With this option, the Carnian/Norian boundary based on conodonts corresponds to basal Newark magnetozone E7 at about 228 Ma (adopting Newark astrochronology), implying a long Norian with a duration of 20m.y. and a Rhaetian of about 6 m.y. duration. These ages are in fact not inconsistent with the few high quality radiometric dates that are available for Late Triassic time scale calibration. We suggest that Pizzo Mondello is a good candidate for a GSSP for the base of the Norian whereas we find that sections of the "Hallstatt" type , which may be more fossiliferous but have erratic and typically very low average rates of sediment accumulation, are more difficult to correlate with each other and with expanded sections such as Pizzo Mondello and the Newark
Il management dell’ipersensibilità dentinale: dalla ricerca alla clinica
No full textObiettivi. Scopo di questo lavoro è presentare le più recenti acquisizioni in tema di epidemiologia, diagnosi, eziopatogenesi e
management clinico dell’ipersensibilità dentinale. Vengono inoltre descritti i prodotti disponibili per il suo trattamento e gli studi
sperimentali e clinici condotti per valutarne l’efficacia. Materiali e metodi. Attraverso la banca dati Medline/Pubmed sono stati
selezionati i lavori originali, le revisioni della letteratura e le linee guida pubblicate in lingua inglese e in italiano sull’argomento
dal 2009 al 2013. Altre fonti sono state selezionate dai riferimenti bibliografici individuati. Risultati e conclusioni. Il management
dell’ipersensibilità prevede il controllo dei fattori causali e l’applicazione domiciliare/professionale di prodotti contenenti agenti
occludenti. Gli studi in vitro non possono riprodurre del tutto i comportamenti quotidiani dei pazienti e le variabili ecologiche del
cavo orale. L’efficacia di tali prodotti pertanto deve essere valutata mediante studi clinici randomizzati e controllati. Il complesso
arginina-carbonato di calcio ha dato prova di efficacia nell’indurre sollievo immediato e duraturo. Sono necessari ulteriori trial
clinici, randomizzati e controllati, per confermare i risultati relativi al fosfosilicato di calcio-sodio e all’acetato di stronzio.Objectives: The aim of this paper is to present an overview on the current state of
knowledge of the epidemiology, aetiology/risk factors, pain mechanisms, diagnosis and
management of dentin hypersensitivity. Available products for treatment of dentin
hypersensitivity are presented and the scientific and clinical research validating their
efficacy is reviewed.
Material and methods: Original papers, reviews and guidelines on this subject, published
in English form 2009 to 2013, were retrieved from Medline/Pubmed database. Additional
publications were obtained by searching the reference list of retrieved works.
Results and conclusions: The management of dentin hypersensitivity is based on the
control of causal factors and the application, at-home or in-office, of various products
which are aimed to occlude patent tubules. In vitro methods suffer from some limitations to
mimicking the “real-life” individual behaviours and the ecological variables of the oral
environment, then randomized and controlled clinical trials are considered the gold
standard to evaluate the efficacy of these products. The arginine-calcium carbonate
complex has demonstrated to be efficacious in providing instant and long-lasting relief.
Further randomized and controlled clinical studies are needed to confirm the results of
amorphous sodium calcium phosphosilicate and strontium acetate
Chelation of cytoplasmic Ca2+ increses plasma membrane permeability in murine macrophages
No full textCytoplasmic free Ca2+ (Ca2+i) was chelated to 10-20 nM in the macrophage cell line J774 either by incubation with quin2 acetoxymethyl ester in the absence of external Ca2+ (Di Virgilio, F., Lew, P.D., and Pozzan, T. (1984) Nature 310, 691-693) or by loading [ethyl-enebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) into the cytoplasm via reversible permeabilization of the plasma membrane with extracellular ATP (Steinberg, T.H., Newman, A.S., Swanson, J.A., and Silverstein, SS.C. (1987) J. Biol. Chem. 262, 8884-8888; Di Virgilio, F., Meyer, B.C., Greenberg, S., and Silverstein, S.C. (1988) J. Cell Biol. 106, 657-666). After removal of ATP from the incubation medium, ATP-permeabilized Ca2+i-depleted macrophages recovered a near-normal plasma membrane potential which slowly depolarized over a 2-4 h incubation at low [Ca2+]i. In both ATP-treated and quin2-loaded cells, depolarization of plasma membrane potential was paralleled by an increase in plasma membrane permeability to low molecular weight aqueous solutes such as eosin yellowish (Mr 692), ethidium bromide (Mr 394), and lucifer yellow (Mr 463). This increased plasma membrane permeability was not accompanied by release of the cytoplasmic marker lactic dehydrogenase for incubations up to 4 h and was likely a specific effect of Ca2+i depletion since it was not caused by: (i) the mere incubation of macrophages with extracellular EGTA, i.e. at near-normal [Ca2+]i; and (ii) loading into the cytoplasm of diethylenetriaminepentaacetic acid, a specific chelator of heavy metals with low affinity for Ca2+. Treatment of Ca2+i-depleted cells with direct (phorbol 12-myristate 13-acetate) or indirect (platelet-activating factor) activators of protein kinase C prevented the increase in plasma membrane permeability. Down-regulation of protein kinase C rendered Ca2+i-depleted macrophages refractory to the protective effect of phorbol 12-myristate 13-acetate. This report suggests a role for Ca2+i and possibly protein kinase C in the regulation of plasma membrane permeability to low molecular weight aqueous solutes
“Salmo salar ribonuclease: structural and functional evidences of an auto inhibition mechanism”
No full textThe pancreatic-type ribonucleases (ptRNases) constitute one of the most investigated family of homologous proteins [1]. PtRNases have been found in various organs and tissues of mammals, amphibians, reptiles, birds [2] and, more recently, fish. In particular, seven new RNases were identified in two teleosts: five from Zebrafish (ZF RNases) and two from Salmo Salar (Ss-RNase-1 and Ss-RNase-2) [3-5]. We have focused our attention on the latter enzyme, which is the most active fish RNase, although its activity is much lower than that of the other members of the ptRNase family.
The crystallographic structure of Ss-RNase-2 (SS2) shows the typical V-shape structure of pancreatic-like ribonucleases, with three helices and six β-strands connected by loops and turns. The electron density in the active site region is discontinuous. In particular, the position of His 113, one of the residues of the catalytic triad that is usually very well defined, cannot be unambiguously identified. Furthermore, the segment Val117-Ile121, which is well-structured and anchored to the protein body, partially obstructs the active site. These structural features suggest that SS2 is in a sort of inhibited state. It is reasonable to hypothesize that, in consequence of an interaction with a specific ligand, the C-terminal segment moves to free the active site. To define the activation mechanism of SS2, we have designed and characterized two deletion mutants: SS2-des117-121, in which the chain segment that in the wild-type protein obstructs the active site has been removed, and SS2-des119-126, in which the elimination of the last eight residues of the chain might allow the sliding of the obstructing segment out of the active site. Interestingly, the crystallographic structure of SS2-des117-121 shows a well-defined active site, almost free of obstructions. Furthermore, the enzymatic activity assays show an improvement of the specific activity of SS2-des117-121 with respect to the wild-type protein. On the bases of these findings, we have proposed an intriguing auto-inhibition mechanism for SS2. The details will be discussed at the Meeting.
[1] L. Aravind, E.V. Koonin Methods Enzymol. 2001, 341, 3.
[2] S. Cho, J.J. Beintema, J. Zhang Genomics. 2005, 85, 208.
[3] E. Pizzo, P. Buonanno, A. Di Maro, S. Ponticelli, S. De Falco, N. Quarto, M.V. Cubellis, G. D'Alessio J Biol Chem. 2006, 281, 27454.
[4] Pizzo, E., Merlino, A., Turano, M., Russo Krauss, I., Coscia, F., Zanfardino, A., Varcamonti, M., Furia, A., Giancola, C., Mazzarella, L., Sica, F., D'Alessio, G. Bioch. J. 2010, 433(2), 345-355
[5] E. Pizzo, M. Varcamonti, A. Di Maro, A. Zanfardino, C. Giancola, G. D'Alessio FEBS J. 2008, 275, 1283
- …
