104,026 research outputs found
G protein-coupled receptor kinase 2-mediated phosphorylation of ezrin is required for G protein-coupled receptor-dependent reorganization of the actin cytoskeleton
No full textG protein-coupled receptor kinase 2 (GRK2) phosphorylates and desensitizes activated G protein-coupled receptors (GPCRs). Here, we identify ezrin as a novel non-GPCR substrate of GRK2. GRK2 phosphorylates glutathione S-transferase (GST)-ezrin, but not an ezrin fusion protein lacking threonine 567 (T567), in vitro. These results suggest that T567, the regulatory phosphorylation site responsible for maintaining ezrin in its active conformation, represents the principle site of GRK2-mediated phosphorylation. Two lines of evidence indicate that GRK2-mediated ezrin-radixin-moesin (ERM) phosphorylation serves to link GPCR activation to cytoskeletal reorganization. First, in Hep2 cells muscarinic M1 receptor (M1MR) activation causes membrane ruffling. This ruffling response is ERM dependent and is accompanied by ERM phosphorylation. Inhibition of GRK2, but not rho kinase or protein kinase C, prevents ERM phosphorylation and membrane ruffling. Second, agonist-induced internalization of the beta(2)-adrenergic receptor (beta(2)AR) and M1MR is accompanied by ERM phosphorylation and localization of phosphorylated ERM to receptor-containing endocytic vesicles. The colocalization of internalized beta(2)AR and phosphorylated ERM is not dependent on Na+/H+ exchanger regulatory factor binding to the beta(2)AR. Inhibition of ezrin function impedes beta(2)AR internalization, further linking GPCR activation, GRK activity, and ezrin function. Overall, our results suggest that GRK2 serves not only to attenuate but also to transduce GPCR-mediated signals
58605: Letter to Mr G. Pitcher informing him of the death of his son, Walter Edward Pitcher
No full textLetter sent by 2/Lt. C. D. B. Cluff, 9th Bn. Durham Light Infantry, to Mr G. Pitcher, father of Pte. Walter Edward Pitcher, informing him of his son's death.
Pte. 325583 (9/7067) Walter Edward Pitcher, 1st/9th Bn. Durham Light Infantry (transferred from the Norfolk Regiment), was killed in action aged 28 on 13th July 1917 and is buried in Wancourt Military Cemetery. Son of George H. and Mary A. Pitcher, of 12, Goldsmith St., Dereham Rd., Norwich.</p
Home of Mr. and Mrs. Daniel G. Pitcher
No full textPhotograph of brick house with large trees in front. Caption on verso reads: “Home place of Mr. & Mrs. Daniel G. Pitcher and daughters Mary & Eva, which was shared with other children during those pioneering church school days, nears Sullivan, Ind., in the ‘Salem’ school.” Sent by Eva Pitcher, Hinsdale Sanitarium, Hinsdale, Ill
Pitcher et al, Cross-modal recognition in goats - playback data
No full textPitcher et al, Cross-modal recognition in goats - playback dat
Letter from Annie G. Pitcher to Mrs. Muir [Louie Strentzel Muir], 1901 Dec 1
No full textBerkely, Dec. 1st 1901. My dear Mrs. Muir: I have thought many times in the past [five] days to be able to acknowledge with many thanks the arrival of the lovely box of grapes which you sent to us. I do not think I ever saw such fine grapes, tho for so many years we lived in a vineyard country. Yesterday commenced one of the heaviest fogs that I ever remember to have [hear], and that it is home. What lightr this evening it still is about us. Many casualties happened on the bay. The sinking of the steamer San Rafael as you no doubt saw in the morning papers bring a fearful accident. I do hope it will prove in have been as fatal o many as they now fear. We were very glad to see Wanda come in safely about three o’clock. We were afraid she could not cross the bay as the boats have been running very irregularly today we heard. With kind remembrance to Miss Helen and many thanks for your kind thoughts in sending the grapes. Most cordially Annie G Aitcher Louie’s letters from friends et
Alien Registration- Pitcher, Gladys G. (South Portland, Cumberland County)
No full texthttps://digitalmaine.com/alien_docs/23162/thumbnail.jp
Pitcher & Manda catalogue of flower and vegetable seeds and bulbs for spring planting
No full text189
Essays on J. L. Austin, (Par) I. Berlin, G. Pitcher, G. J. Warnock, P. F. Strawson, D. F. Pears, J. R. Searle, L. W. Forguson. Édité par I. Berlin. Préface de G. J. Warnock
No full textDe Greef Jan. Essays on J. L. Austin, (Par) I. Berlin, G. Pitcher, G. J. Warnock, P. F. Strawson, D. F. Pears, J. R. Searle, L. W. Forguson. Édité par I. Berlin. Préface de G. J. Warnock. In: Revue Philosophique de Louvain. Quatrième série, tome 71, n°12, 1973. pp. 804-806
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