189,916 research outputs found
CBX2 and DNA damage repair: development of CBX2-specific reagents
No full textDNA damage is inevitable, however, methods of DNA repair exist which allows cells to recover, or die, depending on the severity of the damage. Homologous recombination (HR) is one type of DNA damage repair that has the ability to repair double-stranded breaks (DSBs) in a high-fidelity manner. Unrepaired DSBs can led to cell death or genomic instability.
BRCA1is a tumor suppressor protein that is involved in the cellular response to DNA damage. Studies show that BRCA1 accumulates in S and G2 phases of the cell cycle, and after DNA damage re-localizes to nuclear repair foci. Cells that lack BRCA1 are unable to repair DNA using HR, and therefore tend to use more error-prone mechanisms.
A human auto-antisera, PIKA, first characterized in 2003 by William Earnshaw et al., recognizes proteins which contain chromodomains, and co-localize with BRCA1, before and after DNA damage. Chromodomains are critical features of the HP1 group of proteins, and the polycomb group (PcG) proteins. Prior work has suggested that the protein being recognized by the PIKA anti-sera, was CBX2, a member of the PcG and the polycomb repressive complex 1 (PRC1).
Unfortunately, the antibodies commercially available for CBX2 do not recognize both isoforms of CBX2, the truncated and the full-length. The purpose of this project was to generate plasmid constructs containing coding sequences for the CBX2 full-length protein using the directional cloning technique. A vector encoding a GST-CBX2 SL fusion protein was successfully generated, and conditions for expression and extraction of this protein from bacterial culture were optimized. Progress has been made in isolating a cDNA for the full length CBX2 isoform to generate both GST-fusion proteins and a GFP-tagged protein. By generating a proper CBX2 antibody for the full-length protein, we hope to show that this protein is involved in the normal response to DNA damage.M.S.Includes bibliographical references (p. 46-48)
Denarius - Sydenham 869e - Crawford 408/1a
No full textcf. RRC p.434 Obverse die 136, Reverse die 167. Moneyer is Gaius Calpurnius Piso Frugi, son-in-law to Cicero and son of Lucius Calpurnius Piso, from whom he inherited the Apollo-and-horseman types. Sydenham dates coins in this series to c. 64 B.C.E.C·PISO·L·F·FRVGHead of Apollo with fillet; behind A; border of dotsnaked horseman galloping to left, holding torch in right hand; above, arrow; in exergue, C·PISO·L·F·FRVG; border of dot
Faculdade de Farmácia [da] U. P. : Esquemas de localização de sectores : Cave, 1.º piso, piso intermédio, 2.º piso : 1
No full textDocumentoFaculdade de Farmácia [da] U. P. : Esquemas de localização de sectores : Cave, 1.º piso, piso intermédio, 2.º piso : 1 / M. O. P., Direcção Geral das Construções Escolares, Direcção das Construções Escolares do Norte ; Desenhou P. Silva. - 1:400. - 1982-08-00 - 1982-08-00. - 3 plantas em 1 folha. A data é a do desenho. Apresenta legenda técnica do desenho. N.º do desenho: 354
Denarius - Sydenham 853 - Crawford 408/1b
No full textRRC obverse die 56, reverse die 63 (see RRC I p.428). Moneyer is Gaius Calpurnius Piso Frugi, son-in-law to Cicero and son of Lucius Calpurnius Piso, from whom he inherited the Apollo-and-horseman types. Sydenham dates coins in this series to c. 64 B.C.E.C·PISO·L·F·FRVHead of Apollo with fillet to left; behind, control-mark (S:); border of dotsnaked horseman galloping to right, carrying palm branch over shoulder; above, control-mark (H); in exergue, C·PISO·L·F·FRV; border of dot
Denarius - Sydenham 855b - Crawford 408/1b
No full textFor obverse type and control-mark, see RRC p.426, Obverse die 15. Dr. Badian speculates that the reverse control mark may be an animal, and does not appear in Crawford. Moneyer is Gaius Calpurnius Piso Frugi, son-in-law to Cicero and son of Lucius Calpurnius Piso, from whom he inherited the Apollo-and-horseman types. Sydenham dates coins in this series to c. 64 B.C.E.C·PISO·L·F·FRVHead of Apollo with fillet to right; behind, control-mark (key?); border of dotsnaked horseman wearing conical cap galloping to right, holding palm over left shoulder; above, control-mark; in exergue, C·PISO·L·F·FRV; border of dot
Denarius - Sydenham 841 - Crawford 408
No full textMoneyer is Gaius Calpurnius Piso Frugi, son-in-law to Cicero and son of Lucius Calpurnius Piso, from whom he inherited the Apollo-and-horseman types. Sydenham dates coins in this series to c. 64 B.C.E. Dr. Badian classed this specimen as RRC 408/1, obverse die 62, reverse die 69 (see RRC p.428).C·PISO·L·F·FRVGHead of Apollo with fillet to right; behind, control-mark (sideways M over ·); border of dotsnaked horseman galloping to right; in exergue, C·PISO·L·F·FRVG; below, control-mark (reversed N); border of dot
Denarius - Sydenham 850m? - Crawford 408/1a
No full textRRC obverse die 116, reverse die 143 (see RRC I p. 433 and II plate LXX.97). Moneyer is Gaius Calpurnius Piso Frugi, son-in-law to Cicero and son of Lucius Calpurnius Piso, from whom he inherited the Apollo-and-horseman types. Sydenham dates coins in this series to c. 64 B.C.E.C·PISO·L·F·FRVLaureate or filleted head of Apollo to right; behind, control-mark (50,000 symbol); border of dotshorseman galloping to right, carrying palm branch over shoulder; above, control mark (symbol); in exergue, C·PISO·L·F·FRV; border of dot
Denarius - Sydenham 851h - Crawford 408/1b
No full textPossibly RRC obverse die 24 (dolphin control mark?), reverse die 40 (se RRC I p.426). Moneyer is Gaius Calpurnius Piso Frugi, son-in-law to Cicero and son of Lucius Calpurnius Piso, from whom he inherited the Apollo-and-horseman types. Sydenham dates coins in this series to c. 64 B.C.E.C·PISO·L·F·FRVHead of Apollo, hair tied with band. to right; behind, control-mark (symbol); border of dotsnaked horseman galloping to right, carrying palm branch over shoulder; in exergue, C·PISO·L·F·FRV; below, control-mark (tilted Y or V subscribed with ·); border of dot
Denarius - Sydenham 842c - Crawford 408/1b
No full textRRC obverse die 143, reverse die 174 (see RRC p.434). Moneyer is Gaius Calpurnius Piso Frugi, son-in-law to Cicero and son of Lucius Calpurnius Piso, from whom he inherited the Apollo-and-horseman types. Sydenham dates coins in this series to c. 64 B.C.E. According to Dr. Badian, horseman on obverse may be wearing conical hat.C·PISO·L·F·FRVHead of Apollo with fillet to left; behind, control-mark (bucranium); border of dotshorseman galloping to right; in exergue, C·PISO·L·F·FRV; below, control-mark (symbol); border of dot
Denarius - Sydenham 851m - Crawford 408/1b
No full textPossibly RRC obverse die 30, reverse die 25 (see RRC I p.425). Moneyer is Gaius Calpurnius Piso Frugi, son-in-law to Cicero and son of Lucius Calpurnius Piso, from whom he inherited the Apollo-and-horseman types. Sydenham dates coins in this series to c. 64 B.C.E.C·PISO·L·F·FRVHead of Apollo with fillet to right; behind, control-mark (symbol); border of dotsnaked horseman galloping to right, carrying palm branch over shoulder; above, control-mark (inverted V, possibly with cross-beam); in exergue, C·PISO·L·F·FRV; border of dot
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