88,006 research outputs found
Pirro Ligorio, tra testo e immagine
No full textIntroduzione allo studio delle illustrazioni dei manoscritti di Pirro Ligori
Recensione a F. Rausa, Pirro Ligorio Tombe e mausolei dei Romani
No full textRecensione a F. Rausa, Pirro Ligorio Tombe e mausolei dei Roman
Pirro Ligorio and Sicilian antiquities: indifference or an unwitting ‘short circuit’?
No full textThe antiquarian knowledge of Pirro Ligorio, among the most extensive of his time, shows an important gap concerning the antiquities of Sicily. This seems even more remarkable when we consider that in the period between 1537 and 1573 the most important historical and antiquarian works on the history of Sicily were published by Claudio Mario Arezzo, Tommaso Fazello and Francesco Maurolico. Here, we will try to provide an explanation for this gap and to understand the reasons for it in the light of the facts offered by the cultural context and by the biography of Pirro Ligorio
What Will Decide the U.S. Presidential Elections?
No full textGeorgia Southern faculty member, Robert Pirro, was a podium discussant at John-F.-Kennedy-Institut für Nordamerikastudien, Freie Universität Berli
De Novo Design And Characterization Of A Protein Binding An Abiotic Tetranuclear Zinc Cluster
No full textThe protein matrix finely tunes metal cofactor geometry and reactivity, despite the limited number of ligands.[1] De novo protein design is particularly appealing in gaining new insights on the concurring interactions providing specific functions.[2] While there has been considerable progress in designing proteins that bind mononuclear as well as multinuclear metal cofactors,[3-5] the design of tetranuclear clusters with oxygen-rich environments has not been accomplished. Here, we describe our recent work on the design, structural and spectroscopic characterization of tetra-Zn2+ carboxylate-bridged clusters. The distorted cube-like structure is deeply inserted into the hydrophobic core of a four-helix bundle scaffold, and largely stabilized by a fully connected hydrogen-bonded network, unprecedented in protein design, consisting of 16 polar side chains. By varying the metal-bridging and the interfacial residues in the bundle, we show that subtle changes (in the order of few angstroms) have a great impact on the coordination geometry, as well as oligomeric state and stability. The crystal structures of the first four analogues confirm the validity of the design process in two cases, and define general rules for subsequent designs.[6] Three of these newly designed proteins have been fully characterized by CD and NMR spectroscopies. Solution studies strongly suggest that the desired structure is achieved also in the apo state, providing evidence that the peptide is able to actively impart the designed geometry to the metal cluster.[7]
In perspective, such abiotic tetra-Zn2+ cluster will be adopted as catalyst for both complex transesterification, and hydrolysis reactions.[8] It may also constitute the basis for the construction of the first de novo designed oxygen evolving complex, by replacing zinc with designated redox-active metals.
[1] O. Maglio, F. Nastri, A. Lombardi, in Ioninc Interactions in Natural and Synthetic Macromolecules (Eds.: A. Ciferri, A. Perico), John Wiley & Sons, Inc., Hoboken, NJ, USA, 2012, pp. 361–450.
[2] F. Yu, V. M. Cangelosi, M. L. Zastrow, M. Tegoni, J. S. Plegaria, A. G. Tebo, C. S. Mocny, L. Ruckthong, H. Qayyum, V. L. Pecoraro, Chem. Rev. 2014, 114, 3495–3578.
[3] F. Nastri, M. Chino, O. Maglio, A. Bhagi-Damodaran, Y. Lu, A. Lombardi, Chem. Soc. Rev. 2016, 45, 5020–5054.
[4] A. F. Peacock, Curr. Opin. Chem. Biol. 2016, 31, 160–165.
[5] M. Chino, L. Leone, G. Zambrano, F. Pirro, D. D’Alonzo, V. Firpo, D. Aref, L. Lista, O. Maglio, F. Nastri,
A. Lombardi, Biopolymers 2018, e23107.
[6] S.Q. Zhang, M. Chino, L. Liu, Y. Tang, X. Hu, W. F. DeGrado, A. Lombardi, J. Am. Chem. Soc. 2018, 140, 1294–1304.
[7] M. Chino, S.Q. Zhang, F. Pirro, L. Leone, O. Maglio, A. Lombardi, W. F. DeGrado, Biopolymers 2018, under review.
[8] T. Ohshima, Chem. Pharm. Bull. (Tokyo) 2016, 64, 523–539
A House Divided? The US Presidential Election 2012
No full textGeorgia Southern faculty member, Robert Pirro, was a podium discussant at John-F.-Kennedy-Institut für Nordamerikastudien, Freie Universität Berli
Making Sense of the Tea Party Movement
No full textGeorgia Southern faculty member, Robert Pirro, was a podium discussant at John-F.-Kennedy-Institut für Nordamerikastudien, Freie Universität Berli
The rodents of the Pirro Nord fauna (Foggia, Southern Italy)
No full textThe rodent fauna of various fissure fillings of the Pirro Nord karstic complex are examined here. They have been collected during several field caingns starting from the 70’s until 2006 and are here examined in a comprehensive matter. Hundreds of specimens of arvicolids, murids and, in much lower amount, Glirids have been recovered from several of these fissures. They belong to the species Allophaiomys ex gr. ruffoi, Myodes sp. Apodemus flavicollis and Muscardinus sp
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