1,721,082 research outputs found
Anthocyanins in Catharanthus roseus in vivo and in vitro: A review
No full textThe production of anthocyanin in Catharanthus roseus flowers from both field-grown and regenerated by somatic embryogenesis plants and cell cultures was described. The anthocyanins were identified as the 3-O-glucosides, and the 3-O-(6-O-p-coumaroyl) glucosides of hirsutidin, malvidin and petunidin, respectively both in vivo and in vitro. The influence of environmental conditions on in vitro anthocyanin accumulation is described. The relationship between in vivo and in vitro anthocyanin production is discussed
Characterization of the anthocyanins of Catharanthus roseus (L.) G. Don in vivo and in vitro by electrospray ionization ion trap mass spectrometry
No full textThe power of direct infusion electrospray (ESI) mass spectrometry and ion trap multiple mass spectrometry (MS/MS) were applied to the detection and identification of the anthocyanic compounds of Catharanthus roseus (L.) G. Don. Cell suspensions of C. roseus, fresh flowers from both in vitro regenerated and field-grown plants, were comparatively examined. The anthocyanic extracts were analyzed by positive-ion electrospray ionization mass spectrometry and collision experiments were performed on molecular ions by means of ion trap facilities. In parallel, classical approaches such as thin layer chromatography (TLC) and UV/Vis spectroscopy were undertaken. Petunidin, malvidin and hirsutidin, already known in the literature, were identified as aglycones. Three 3-O-glucoside derivatives, supposed to be present on the basis of literature data, were also identified. Moreover, three 3-O-(6-O-p-coumaroyl)glucosides were identified for the first time in this species. Using the direct ESI-MS/MS approach, the mass spectra of all the anthocyanic compounds present in the extracts can be obtained, even of those occurring in traces and not detectable by TLC. The three extracts investigated showed the same qualitative anthocyanic profile, but large quantitative difference
Il destino metabolico di un substrato: disegno unico o percorsi diversi in vivo e in vitro?
No full textSomatic embryogenesis and glucosinolate/myrosinase system in vulnerable Brassica repanda subsp. glabrescens (Poldini) Gómez-Campo
No full textCoumarin compounds in Coronilla scorpioides callus cultures.
No full textCoronilla scorpioides (L.) W.D.J. Koch is known for producing several compounds with pharmaceutical interest, such as the hydroxycoumarins umbelliferone, scopoletin and daphnoretin, the dihydrofuranocoumarin marmesin, and the furocoumarin psoralen. In vitro callus cultures of C. scorpioides were established from hypocotyl, leaf, stem internode and root explants in order to evaluate the possibility of in vitro production of these active secondary metabolites. Calli were obtained with high frequency from all the explant types both in B5 and MS medium. However, after the third subculture, B5 medium, giving the best results, was selected for subsequent transfers. Homogeneous calli were kept either in darkness or in light. Chemical analyses showed that scopoletin and the intermediate products of the biogenetic pathway of psoralen, umbelliferone and marmesin, were always present in the calli and excreted into the media, while daphnoretin was never detected. Light seems to be a prerequisite for psoralen biosynthesis. Root-derived calli produced a significantly higher amount of psoralen (137.5 microg g(-1) DW). Principal component analysis showed that umbelliferone, marmesin and psoralen contents are related to variables associated with different explant type
Production of secondary metabolites with plant cells immobilized in a porous inorganic support
No full textProduction of secondary metabolites with plant cells immobilized in a porous inorganic support
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Colture in vitro di cellule vegetali accoppiate alla sintesi chimica per la produzione di composti di interesse farmaceutico, cosmetico e alimentare
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