77 research outputs found
An Overview of MSME Sector in India with Special Reference to the State of Uttarakhand
No full textMSMEs, considered as "Engines of Growth" are crucial for the economy of developing countries like India due to its potential of creating vast employment opportunities. This Sector has caught the attention of policy makers as it is labour intensive and has the potential of reducing the problem of regional imbalances, ensure financial independence of rural people and assist in sustainable development. The Micro, Small and Medium Enterprises in India is being largely viewed as vital to the economic growth of the country in the 21st century. The present paper will focus on existing scenario and trends of MSME in India. This paper studies the current status of MSME sector in India, its performance, various initiatives taken by the government to accelerate the growth of this sector, as well as the problems faced by the MSMEs in Indian economy. Additionally, this paper dedicates one section to the MSMEs sector in the state of Uttarakhand. The State of Uttarakhand is facing the dire problem of intense migration of people from the hilly terrain particularly due to lack of employment opportunities in the rural areas of Uttarakhand and Uttarakhand badly needs sustainable and inclusive industrial growth of all sub regions of the state. Hence, this paper also analyses the status of MSMEs in the state of Uttarakhand and outlines the problems faced and also recommends the measures that should be taken to promote this sector. The study is a review which was conducted with the help of secondary data retrieved from MSME Annual Reports, other government reports, newspapers and research papers. Simranjeet Kaur Virk | Pinnacci Negi "An Overview of MSME Sector in India with Special Reference to the State of Uttarakhand" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-2 , February 2019, URL: https://www.ijtsrd.com/papers/ijtsrd21520.pd
Mechanisms of Antibacterial Activity of the Fruit and the Bark Extracts of Dillenia Indica
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Studies on Molecular Detection of Staphylococcus Species
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Identification of Staphylococcus Species by using Species Specific Primers.
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Isolation, Characterisation And Antimicrobial Activity Of Zingiber Zerumbet L.
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Mechanism of Bioactivity of Peltophorum Ferrugineum Flower Extracts
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Antibacterial mechanisms of the fruit and bark extracts of Dillenia indica
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Regulatory dynamics of transcription factors TBX18 and WT1 in mouse prostate development
No full textThe T-box transcription factor TBX18 is essential to mesenchymal cell differentiation in several tissues and Tbx18 loss-of-function results in dramatic organ malformations and perinatal lethality. Although the expression and function of Tbx18 has been analyzed in several tissues, the homozygous mutants die at birth, and the long-term effects of Tbx18 in cell development and differentiation have not been identified. Using a long-lived translocation mutant, 12Gso - a hypomorphic allele of Tbx18 caused due to disruption of the gene’s regulatory structure - we were able to circumvent the perinatal lethality and investigate adult phenotypes of Tbx18 deficiency. Upon studying the histopathology of 12Gso/Tbx18- mutant adults, unexpectedly severe defects were seen in the prostate. The prostate became an ideal system to study Tbx18’s role in postnatal development due to its non-essential nature, thus giving us the ability to create conditional mutants of Tbx18 in this tissue that survive to adulthood.
Our studies demonstrated that Tbx18 is essential to the development of periductal smooth muscle stromal cells in prostate, particularly in the anterior lobe, with a clear impact on prostate health in adult mice. Prostate mesenchymal cells that fail to express Tbx18 do not condense normally into smooth muscle cells of the periductal prostatic stroma; these periductal stromal cells in mutant prostate assume a hypertrophic, myofibroblastic state and the adjacent epithelium becomes grossly disorganized. To identify molecular events preceding the onset of this pathology, we compared gene expression in the urogenital sinus (UGS), from which the prostate develops, in Tbx18-null and wild type littermates at two embryonic stages. Genes that regulate cell proliferation, smooth muscle differentiation, prostate epithelium development, and inflammatory response were significantly dysregulated in the mutant urogenital sinus around the time that Tbx18 is first expressed in the wild type UGS, suggesting a direct role in regulating those genes.
Our previous data indicated that the dynamic expression of Tbx18 is controlled by an expansive regulatory architecture, extending far upstream and downstream of the gene. We coupled circularized chromatin conformation capture (4C) and ATAC-seq from embryonic day 18.5 (E18.5) mouse urogenital sinus (UGS), with the goal of identifying elements that interact with the Tbx18 promoter in developing prostate. The data revealed dozens of active chromatin elements distributed throughout a 1.5 million base pair topologically associating domain (TAD). To identify cell types contributing to this chromatin signal, we used lineage tracing methods with a Tbx18:Cre “knock-in” allele; these data show clearly that Tbx18-expressing precursors differentiate into wide array of cell types in multiple tissue compartments, most of which had not been previously reported. We also used a 209 kb Cre-expressing Tbx18 transgene (Tbx18:BAC-iCre), to partition enhancers for specific precursor types into two rough spatial domains.
RNA-seq studies of the developing UGS revealed expression of the zinc-finger transcription factor Wt1, a gene previously unreported to be expressed in the prostate and a known interaction partner of Tbx18. Furthermore, Wt1 was significantly dysregulated in Tbx18-/- mutant UGS indicating a relationship between the two genes in this tissue. After establishing high expression of Wt1 in the developing UGS and tracing the cell lineages arising from Wt1-expressing precursor cells, we obtained a conditional knockout mutation of the Wt1 gene and tested the effects of Wt1 loss of function in prostate development. Crossing the conditional mutants with animals carrying a prostate-specific Cre allele, we identified a severe over-proliferative phenotype in the prostate of the conditional Wt1 knockouts. To identify transcriptional targets of this TF, we performed WT1 chromatin immunoprecipitation followed by high throughput sequencing (ChIP-seq) in UGS chromatin using a tested WT1 antibody. The data revealed a role for Wt1 upstream of the TGFβ/BMP pathway during prostate development, a signaling pathway known to inhibit over-proliferation of epithelial cells. Whereas WT1 has been known for some time to be dysregulated in human prostate cancer, its function in the normal prostate has not previously been described. This study has thus revealed a new role for the very well-studied WT1 protein, as a transcription factor with a key function in prostate development, and one with clear relevance to a potential link to prostate cancer in adults.
This thesis uses a combination of traditional cell biology approaches along with functional genomics strategies to study the expression and function of the transcription factors, Tbx18 and Wt1, in prostate development for the first time. Using mouse genetics, we generate conditional mutants to identify histo-pathologies associated with the loss-of-function of these genes in the prostate. RNA-seq and ChIP-seq are also used to identify molecular targets and pathways downstream to these TFs. Finally, ATAC-seq and 4C are used to map the extended regulatory network that controls expression of Tbx18 in the particular cell fates.Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2021-05-01The student, Soumya Negi, accepted the attached license on 2019-04-12 at 10:19.The student, Soumya Negi, submitted this Dissertation for approval on 2019-04-12 at 10:33.This Dissertation was approved for publication on 2019-04-14 at 11:56.DSpace SAF Submission Ingestion Package generated from Vireo submission #13583 on 2019-08-22 at 16:21:05Made available in DSpace on 2019-08-23T20:44:43Z (GMT). No. of bitstreams: 5
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Previous issue date: 2019-04-14Embargo set by: Seth Robbins for item 112303
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Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 112303
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Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 112303
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Antibacterial Activity of Eugenol in Model Food Systems.
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
“Evaluation of biological activities of Plectranthus ambonicus leaf extracts.
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
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