1,720,988 research outputs found
In vitro production of free oxygen radicals induced by pulsed ultrasounds in whole blood exposed to diagnostical frequencies and intensities
No full textDNA alkalinization experiments on lymphocytes from sonicated whole blood and on in vitro cultured lymphocytes in presence of free radical scavengers (superoxide dismutase, catalase and mannitol) showed that lesions inflicted upon DNA by pulsed ultrasounds could be ascribed to production of free radicals (O2-, OH.) and H2O2, which could mediate the production of still unidentified organic radicals, likely to be responsible for DNA damage
Effects of pulsed ultrasound and ultraviolet radiation on vitamin E and olive oil
No full textThe effects of ultrasound and ultraviolet radiation on Vitamin E and its pharmacological excipient, olive oil, were tested by fluorescent analysis of DNA unwinding (FADU) on DNA extracted from human lymphocytes of healthy donors. The results show that Vitamin E may be inactivated and behave as a radical species, while olive oil appears unaffected by treatment either with ultrasound or with ultraviolet radiatio
Production of reactive oxygen species in microsomes isolated from peripheral blood mononuclear cells of patients affected by allergic asthma
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Sonicated serum damages human lymphocyte DNA: a model of free radicals induced cell injury
No full textAnalisi della produzione di specie reattive dell’ossigeno in cellule cybride di pazienti affetti da neuropatia ottica ereditaria di Leber (LHON)
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Biological effects on DNA and on ultrastructure of fetal white blood cells (WBC) from mothers monitored by continuous ultrasounds
No full textIn order to investigate biological effects of ultrasounds on blood cell DNA, a biochemical study was undertaken at the ultrastructural and molecular level on lymphocytes from babies whose delivery was directed through ultrasound monitorage and babies whose mother had undergone ultrasound monitorage 48 hours before delivery. Our preliminary results show that the ultrasounds currently employed in diagnostics and monitorage can induce upon DNA in vivo reversible damages, as mentioned before, while ultrastructural alterations are still detectable 48 hours after sonicatio
Reactive oxygen species in human inner ear perilymph
No full textConclusions: The results reported here provide the first evidence of the production of superoxide, a biologically relevant reactive oxygen species (ROS), in human inner ear perilymph (hIP) in pathological conditions, by the activity of the xanthine dehydrogenase/xanthine oxidase (XA/XO) enzyme system. Objective: To investigate the presence of ROS in hIP. Methods: Since damage and apoptosis of inner ear hair cells may occur as a result of ROS-mediated injury, we investigated the presence and production of ROS in 105 hIP samples; 98 collected from patients affected by profound sensorineural hearing loss, during surgery for cochlear implantation, and 7 controls, collected from patients affected by otosclerosis, in case of spontaneous leakage after stapedotomy. ROS production was investigated by spectrophotometric analysis and polyacrylamide gel electrophoresis (SDS-PAGE). Results: In hIP samples tested by cytochrome c reduction kinetics, the average superoxide production was 27.34 mmoles per mg of total protein, against 0.36 in controls. Some of these hIP samples, analyzed by cytochrome c reduction kinetics in the presence of xanthine, were found to be positive for ROS-producing XA/XO enzyme. These results were supported by SDS-PAGE analysis
Very low density lipoproteins mediate DNA damage induced by sonicated serum on human lymphocytes.
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