13,043 research outputs found
Intravital laser-scanning microscopy for biomedical research
No full textWe developed a custom-design video-rate intravital laser-scanning microscopy system for direct in vivo cellular visualization. We visualized the dynamic behavior of flowing T lymphocytes in lymph node and the molecular absorption in small intestine
In vivo quantitation of circulating tumor cells by video-rate intravital laser-scanning confocal microscopy
No full textWe established an intravital high-speed laser-scanning confocal microscopy capable of visualizing fast-flowing individual cells at great saphenous vein of mouse model and quantified circulating tumor cells of whole body blood, in vivo, by image-based analysis
Implementation of performance measurement of a novel in-service supervisory system for WDM transmission link
No full textA Novel Pancreatic Imaging Window for Stabilized Longitudinal In Vivo Observation of Pancreatic Islets in Murine Model
No full textLongitudinal imaging of murine pancreas is technically challenging due to the mechanical softness of the tissue influenced by peristalsis. Here, we report a novel pancreatic imaging window for long-term stabilized cellular-level observation of the islets in the pancreas in vivo. By spatially separating the pancreas from the bowel movement and physiologic respiration with a metal plate integrated in the imaging window, we successfully tracked the pancreatic islets up to three weeks and visualized the dumbbell-shape transformation from the single islet. This window can be a useful tool for long-term cellular-level visualization of the microstructure in the pancreas.
생체 내 유방조직 미세영상 획득을 위한 윈도우 장치 및 이를 이용한 영상 획득 방법
No full textThe invention discloses a window device for acquiring a microscopic image of breast tissue in a living body and a method for acquiring an image by using the window device. According to an embodiment of the present invention, there is provided a window device, which has an open window formed in the center and has a ring structure, a cover glass is provided on the upper end, and a 1 st chamber for placing breast tissue is provided on the lower end; a 2 nd chamber centrally forming an open window and associated with the 1 st chamber and holding the breast tissue; and a chamber bracket which fixes the 1 st chamber and the 2 nd chamber, forms a tilting base placing part and is provided with a tilting base capable of keeping the parallel state of the cover glass and the objective lens of the confocal microscope system. According to the present embodiment, there is an advantage in that a microscopic image at the cellular and molecular level of a tissue in vivo can be stably obtained for a long period of time while maintaining the environment of an animal in vivo
Intravital Imaging of Circulating Red Blood Cells in the Retinal Vasculature of Growing Mice
No full textPurpose: To establish a custom-built, high-speed 90 frame-per-second laser-scanning confocal microscope for real-time in vivo retinal imaging of individual flowing red blood cells (RBCs) in retinal vasculature of live mouse model. Methods: Fluorescently labeled RBCs were injected into mice of different ages (3 to 62 weeks old). Anti-CD31 antibody conjugated with Alexa Fluor 647 was injected to visualize retinal endothelial cells (ECs). Longitudinal and cross-sectional intravital retinal imaging of flowing RBCs and ECs was performed in two strains (C57BL/6 and Balb/c) by using the custom-built confocal microscope. Results: Simultaneous tracking of the routes of many fluorescently labeled individual RBCs flowing from a large artery and vein to a single capillary in the retina of live mice was achieved, which enabled in vivo measurement of retinal RBC flow velocities in each vessel type in growing mice from 3 to 62 weeks after birth. Average RBC flow velocities were gradually increased during growing from 3 to 14 weeks by more than two times. Then the average RBC flow velocity was maintained at about 20 mm/s in artery and 16 mm/s in vein until 62 weeks. Conclusions: Our study successfully established a custom-built high-speed 90-Hz retinal confocal microscope for measuring RBC flow velocity at the single cell level. It could be a useful tool to investigate the pathophysiology of various retinal diseases associated with blood flow impairment. Translational Relevance: This technological method could be a valuable assessment tool to help the development of novel therapeutics for retinal diseases.
생체 내 폐조직 미세영상 획득을 위한 미세흡인 기반 폐 윈도우 장치 및 이를 이용한 영상 획득 방법
No full textThe invention discloses a lung window device based on micro inhalation for acquiring a lung tissue microscopic image in a living body and a method for acquiring the image by using the lung window device. According to an embodiment of the present invention, there is provided a pulmonary window device including an open window having an upper end and a lower end communicating with each other, a cover glass provided on the upper end and a lower end contacting a pulmonary tissue, a suction tube extending from one side of the open window to a suction device and forming a vacuum state in the open window, and a tilt base placing section extending from one side of the open window and having a tilt base placed thereon for keeping the cover glass and an objective lens of a confocal microscope system in a parallel state. According to this embodiment, there is an advantage in that it is possible to stably acquire microscopic images at the cellular and molecular levels of lung tissue in vivo while maintaining physiological respiration and circulation of animals without interfering with this
Frequency selection rule for high definition and high frame rate Lissajous scanning
No full textAbstractLissajous microscanners are very attractive in compact laser scanning applications such as endomicroscopy or pro-projection display owing to high mechanical stability and low operating voltages. The scanning frequency serves as a critical factor for determining the scanning imaging quality. Here we report the selection rule of scanning frequencies that can realize high definition and high frame-rate (HDHF) full-repeated Lissajous scanning imaging. The fill factor (FF) monotonically increases with the total lobe number of a Lissajous curve, i.e., the sum of scanning frequencies divided by the great common divisor (GCD) of bi-axial scanning frequencies. The frames per second (FPS), called the pattern repeated rate or the frame rate, linearly increases with GCD. HDHF Lissajous scanning is achieved at the bi-axial scanning frequencies, where the GCD has the maximum value among various sets of the scanning frequencies satisfying the total lobe number for a target FF. Based on this selection rule, the experimental results clearly demonstrate that conventional Lissajous scanners substantially increase both FF and FPS by slightly modulating the scanning frequencies at near the resonance within the resonance bandwidth of a Lissajous scanner. This selection rule provides a new guideline for HDHF Lissajous scanning in compact laser scanning systems.</jats:p
Closed integral form expansion of Raman equation for efficient gain optimization process
No full textIn this study, we introduce a closed integral form of coupled Raman equation set, which enables us to reduce the complex multipump Raman gain design problem into a series of matrix calculations. With simultaneous implementation of signal stimulated Raman scattering effect and Raman integration in single iteration step, fast convergence with excellent accuracy has been achieved
In vivo observation of multi‐phase spatiotemporal cellular dynamics of transplanted HSPCs during early engraftment
No full textHematopoietic stem cell transplantation (HSCT) is commonly used to treat patients with various blood disorders, genetic and immunological diseases, and solid tumors. Several systemic complications following HSCT are critical limiting factors for achieving a successful outcome. These systemic complications are mainly due to the lack of initial engraftment after transplantation. However, the detailed underlying cellular dynamics of early engraftment have not been fully characterized yet. We performed in vivo longitudinal visualization of early engraftment characteristics of transplanted hematopoietic stem and progenitor cells (HSPCs) in the mouse calvarial bone marrow (BM). To achieve this, we utilized an in vivo laser‐scanning confocal microscopy imaging system with a cranial BM imaging window and stereotaxic device. We observed two distinct cellular behaviors of HSPCs in vivo, cluster formation and cluster dissociation, early after transplantation. Furthermore, we successfully identified three cellular phases of engraftment with distinct cellular distances which are coordinated with cell proliferation and cell migration dynamics during initial engraftment
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