23,557 research outputs found
Sheep and sheep herders near Cady Pass, Snohomish County, Washington, 1930
No full textCaption on image: 2000 Sheep on bench mark Mt. near Cady Pass. Pickett 4639To order a reproduction, inquire about permissions, or for information about prices see: http://www.lib.washington.edu/specialcollections/services/reproduction/reproduction Please cite the Order Numbe
Sheep and sheep herders near Cady Pass, Snohomish County, Washington, 1930
No full textCaption on image: Sheep near Cady Pass. Snohomish Co. Wash. Showing forest fire look out on bench mark Mt. elev 6000 ft. 2000 Sheep. Pickett 4641To order a reproduction, inquire about permissions, or for information about prices see: http://www.lib.washington.edu/specialcollections/services/reproduction/reproduction Please cite the Order Numbe
Regulatory aspects of the Gua operon of Escherichia Coli
No full textThe mechanisms regulating expression of the guanine operon were investigated in a number of ways. (1) Gua-lac fusions were constructed in vivo using the specialised transducing phage Mud(Ap, lac). Insertion of Mud(Ap, lac) into guaA and subsequent selection for temperature resistance, yielded a recombinant, MP1004, in which the genes of the lac operon are controlled as an integral part of the gua operon, the promoter proximal guaB gene remaining intact. Upon guanine starvation of MP1004, IMP dehydrogenase (the product of the guaB gene) expression derepresses 50-fold and β-galactosidase expression simultaneously derepresses five-fold. Spontaneous and NG-induced mutations, causing defective gua control, were sought in MP1004. Mutants with constitutively derepressed levels of β-galactosidase or lactose permease were selected on minimal lactose or melibiose plates, respectively, and arose at a frequency of c.10-5. The 24 mutants tested exhibited β-galactosidase levels raised up to 2.5-fold, but none showed IMP dehydrogenase levels raised more than 1.3-fold relative to the parent strain, MP1004. These mutants did not arise by Mud(Ap, lac) phage transposition. (2) A closer fusion of the gua and lac genes was accomplished in vitro utilising the promoter cloning plasmid pMC1403. A 2.1kb EcoRI fragment containing the gua promoter and the promoter proximal portion of guaB, was inserted into pMC1403. Correction of reading frame using exonuclease BAL31 yielded two recombinant plasmids, pMP108 and pMP112, which encoded a 140,000 mol.wt. hybrid protein consisting of an enzymatically active 116,700 mol.wt. β-galactosidase with 26,000 mol.wt. fragment (encoded by the promoter proximal portion of guaB) joined at its N-terminus. When the appropriate lac host containing either of these recombinant plasmids was starved for guanine, a six fold derepression of β-galactosidase activity was observed. a 50% repression of β-galactosidase activity was elicited by excess guanine. Thus, the genes of the lac operon are under control of the gua promoter in the plasmids pMP108 and pMP112. Attempts to isolate mutants with constitutively derepressed levels of β-galactosidase or lactose permease, were unsuccessful. (3) A putative repressor binding site overlaps the Pribnow box of the primary gua promoter. Oligonucleotide-directed mutagenesis was used to create an A to T transversion that disrupts this region of hyphenated dyad symmetry. The activities of wild-type and mutant gua promoters were compared by cloning a 310bp AluI fragment containing the promoter into the galactokinase promoter cloning plasmids, pK04 and pK06. Although the mutation caused no change in gua promoter activity, in this system even the wild-type promoter failed to respond to stimuli normally causing derepression or repression of the gua operon. (4) The DNA sequence of the gua promoter reveals several features that suggest stringent control of the operon. A relA^+/relA^- isogenic pair of strains was used to investigate such a mechanism. A `relaxed'' strain (relA^-) exhibited a 26% decrease in IMP dehydrogenase levels when starved for isoleucine. When the stringent response occurred, only a 10% decrease was observed under the same conditions. The transcriptional activity of the gua promoter was measured by hybridising labelled gua transcripts to λpguaA DNA. When the wild-type strain (W3110) was grown in medium containing excess guanine, an eight-fold repression of IMP dehydrogenase activity but only a two-fold repression of transcriptional activity, occurred. Starvation for isoleucine in a `relaxed'' strain caused a two-fold increase in transcriptional activity of the gua promoter; no such increase was observed when the stringent response occurred.</p
The sense of a beginning : Bakhtinian dialogic criticism on 'the gospel' in Mark.
No full textContemporary literary approaches have caused paradigm shifts in Biblical Studies in the last two decades as it appears in a great deal of Markan studies using narrative, reader-response, deconstructive, feminist, and new historicist approaches. However, literary studies on the Gospel of Mark have not taken into account theoretical questions underlying those approaches. As a result biblical critics are driven by new trends without ever having a chance to examine the critical baggage of the approaches. Consequently, there is a gap of communication between the old and the new one. Therefore this thesis is an attempt to meet the need of enhancing the quality of critical endeavour in biblical studies. In the light of most recent competing critical theories of literature, the first contribution of this thesis is the methodological finding that Bakhtinian dialogic criticism contains the most profound philosophical and practical foundations for solving some crucial theoretical problems in contemporary literary theories. It is a critique to a Saussurian linguistic system of language which becomes the very foundation of modern and postmodern literary criticism. Bakhtinian literary theory shifts the foundation of literary criticism on linguistic signs into the creative activity of the socio-cultural production of human communication. The shift into socio-cultural reality of language communication makes the notion of 'genre' very important to unlock the problem of text and context in literary studies. Since the Gospel of Mark has fascinated most literary critics in Biblical Studies, the problem of 'genre' of this gospel is chosen as the focus of this study. Secondly, as no agreement is reached as to what 'genre' the Gospel of Mark belongs, this thesis makes its contribution to the discussion by locating the problem of 'genre' of Mark in the context of genre theories and argues that the Bakhtinian suggestion to find genre in the socio-cultural sphere by analysing artistic intercourse between narrative agents in Mark has freed the competing analysis from the unresolved problem between the kerygmatic (content oriented) approach and the analogical (form oriented) approach. To achieve finding 'genre' in the socio-cultural sphere, this thesis focuses on Bakhtinian analysis of the process of artistic intercourse between narrative agents. The narrative communicative interrelationships between narrative agents is constructed in this thesis as a 'stereophonic' Bakhtinian model of dialogic communication. This model is an original contribution of this thesis for revising the traditional two dimensional model of narrative communication. Based on this dialogical model of communication, a special role is given to the Bakhtinian 'author-creator' in the realization process of genre through the interaction of polyphonic voices. Through the interaction of voices of the author-artist and the hero we are led to discover a relatively stable type of portraying and controlling reality in Mark, known as the genre of Roman 'satire'. The closest literary affinity is Satyrica by Petronius. This narrative strategy of 'satire' in Mark has its root in the prophetic discourse of the Old Testament which is saturating the speech of the narrator, John the Immerser, the centurion, the people, and even Jesus. Finally, the whole search for Markan 'genre' culminates in the analysis of the realization of genre through the analysis of Bakhtinian chronotope. The reality of the genre of Mark is its social reality that is in its role as dpxrj/ 'beginning'. As the Gospel of Mark proclaims itself as 'a beginning', it defines its claim of socio-cultural 'authority' in early Christianity. It is this 'sense of beginning' which enables the narrating and the narrated world of Mark to interact dialogically
Pickett Springs, Montgomery, Alabama
No full textColor print view of Pickett Springs in Montgomery, AL. Wooden area with circular brick wall around trees. Small shelter-type structure near trees. Larger structure on hill in background. People sitting under a tree. Divided back postcard.Printed on front: 'Pickett Springs, Montgomery, Ala.' Printed on back: 'No. C. 11303 Pub. By A. Gibian, Montgomery, Ala. Printed on Germany. Litho-chrome Trade-mark
Democracy Sausage with Mark Kenny: How to be a liberal with Ian Dunt
No full textOn this Democracy Sausage Extra, Ian Dunt - host of the Oh God, What Now? podcast and author of How to be a liberal - joins Mark Kenny to discuss the history of liberal thought, how it has shaped present day politics, and the origins of the ‘culture wars’. Have the culture wars emerged out of the failures of liberalism? Why haven’t contemporary political actors done more to protect people from prejudice and the tyranny of the majority? And is liberalism a natural corollary to democracy? On this Democracy Sausage Extra, author, political journalist and broadcaster Ian Dunt joins Professor Mark Kenny to discuss the history of political thought, present day politics, and liberalism’s trajectory
[Interview with Mark Lane in Playboy Magazine #3]
No full textPoor quality photocopies of a magazine article which appeared in Playboy Magazine. The article features an extensive interview with Mark Lane, an attorney and author, who is critical of the Warren Commission's assessment of the assassination of President Kennedy
Democracy Sausage with Mark Kenny: Full circle with Scott Ludlam
No full textOn this episode of Democracy Sausage, Scott Ludlam, former Greens Deputy Leader and author of the new book Full Circle: A search for the world that comes next, joins Mark Kenny to discuss what he learnt from his time in politics and Australian climate policy. What role do corporate and private interests play in shaping Australian policy-making? Will the country make changes to political donation rules to make the system more transparent? And how can Australia make meaningful progress on climate policy? On this episode of Democracy Sausage, Professor Mark Kenny speaks with former Greens Senator Scott Ludlam about Australian politics, his new book, and Section 44 of the Constitution
Democracy Sausage with Mark Kenny: Truth is trouble with Malcolm Knox
No full textOn this Democracy Sausage Extra, award-winning journalist and author Malcolm Knox joins Mark Kenny to discuss the saga of Israel Folau - former star rugby union player sacked for sharing anti-LGBTQ views on social media - and how free speech got so complicated. The sacking of former star player Israel Folau by Rugby Australia for his comments on social media once again revealed faultlines which had recently been laid bare during Australia’s marriage equality plebiscite. So what did the saga reveal about freedom of expression in Australia? What is the significance of groups like the Australian Christian Lobby in Australia’s public discourse? And, with ‘free speech’ very much a political battleground, what might the future hold? On this Democracy Sausage Extra, Professor Mark Kenny speaks with Australian journalist and author Malcolm Knox about the Israel Folau issue, Australia’s evangelical movement, and the ‘culture wars’. This episode was recorded live as part of the ANU/Canberra Times ‘Meet the Author’ series
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