7,143 research outputs found
A bacterium from the human microbiota as a vaccine vector. Efficient priming of the murine immune system by vaginal delivery of recombinant Streptococcus gordonii
No full textThe ability to prime the immune system against an antigen, and to rapidly recall this response upon antigen reencounter is a fundamental characteristic of the adaptive immune response. The association of an antigen recognized by the immune system in a certain tissue, with the same antigen encountered at a later timepoint in a different tissue, is of primary importance to obtain a systemic and effective immune response and is the foundation behind the utilization of vaccines. The study of vaccine delivery platforms that may activate the immune system in such a manner is therefore of primary importance in the effort to design novel vaccine formulation and prime-boost strategies.
The aim of the present work was to study the in vivo priming effect induced by a recombinant Streptococcus gordonii vaccine vector expressing a heterologous antigen and delivered to the vaginal tract, a unique mucosal tissue.
To study the priming effect induced by the recombinant Streptococcus gordonii, we employed a prime-boost strategy and compared the cellular an humoral immune response towards the soluble antigen between recombinant- and WT-immunized mice. Using this model, we show that vaginal immunization with the recombinant Streptococcus gordonii elicited systemic production of antigen-specific antibodies, shifted the IgG subclasses profile, led to an increase in plasma cells in the lymph nodes, a higher number of antigen-specific antibody-secreting cells in the spleen and modulated the cytokine expression profile of splenocytes. The longevity of the priming effect induced by the recombinant vector was also analyzed by comparing three and six months boosting schedules. We found that the priming is boostable with a similar efficacy for at least six months (Chapter 3). These data demonstrate that vaginal priming with the recombinant S. gordonii vector results in a systemic activation of both cellular and humoral immune compartments, and that this priming effect is long-lived without significant immune waning.
In this study, we also assessed the transcriptomic response of splenocytes from S. gordonii-immunized mice towards the antigen. We observed differences in immune pathways between recombinant- and WT-immunized mice, and also between the three- and six-months boosted groups (Chapter 4). In addition, we observed a gene signature correlated with antigen-specific IgG titers. These findings suggest that the immune system’s encounter with the antigen on the surface of the recombinant S. gordonii in the vaginal tract results in a differential immune activation in in response to the antigen.
This work contributes to the knowledge on the capability of recombinant live vaccine vectors delivered mucosally to prime and modulate the immune response, and has important implications in the design of innovative vaccination strategies
Syriac-Arabic Glosses of Isho bar Ali. Volume 1
No full textThese two volumes constitute the second part (nun-taw) of the Syriac-Arabic dictionary of the 10th cent. physician Isho bar Ali (the first half of the dictionary had been published in 1874 by G. Hoffmann). Each Syriac word is defined in Arabic, often with more than one Arabic equivalent; in addition, the author deals not just with individual Syriac words, but in some cases with phrases. Gottheil used 21 manuscripts (from Oxford, London, Paris, Berlin, Leiden, and Rome) for this edition, and he has supplied a thorough critical apparatus; the manuscripts are described in the introduction. While some manuscripts give the Arabic glosses in Syriac characters (i.e. Garshuni), Gottheil has presented them here in Arabic script. These two volumes will be of great interest to Syriac lexicographers and those who study interactions between Syriac and Arabic.Contains an English introduction by Richard J.H. Gotthei
Syriac-Arabic Glosses of Isho bar Ali. Volume 2
No full textThese two volumes constitute the second part (nun-taw) of the Syriac-Arabic dictionary of the 10th cent. physician Isho bar Ali (the first half of the dictionary had been published in 1874 by G. Hoffmann). Each Syriac word is defined in Arabic, often with more than one Arabic equivalent; in addition, the author deals not just with individual Syriac words, but in some cases with phrases. Gottheil used 21 manuscripts (from Oxford, London, Paris, Berlin, Leiden, and Rome) for this edition, and he has supplied a thorough critical apparatus; the manuscripts are described in the introduction. While some manuscripts give the Arabic glosses in Syriac characters (i.e. Garshuni), Gottheil has presented them here in Arabic script. These two volumes will be of great interest to Syriac lexicographers and those who study interactions between Syriac and Arabic.Contains an English introduction by Richard J.H. Gotthei
Assessment of Dowel Bar Research;HR-1080, August 2002
No full textThis report presents the latest technological gaps in dowel bar research based upon completed and ongoing dowel bar research from across the nation. In order to obtain this collection of information about dowel bars, a search was conducted on a nationwide level. The technological gaps and duplications of the research were then determined. In addition, this report also provides a brief annotated bibliography of all sources used to determine the gaps in technology and knowledge for dowel bar and alternative dowel bar topics as applied to highway pavements
A matrix of measures of court workload
No full textPerformance measures depend on the clarity and consistency of their metrics. The standard unit of analysis of court proceedings is the “case.” Yet the definition of a case varies widely. It may include multiple accused persons and charges, a count of accused persons, the number of informations (a set of charges), or the number of charges. Likewise, whether new cases, completed cases, or the number of cases heard in court are counted make a tremendous difference. In theory, many dozens of caseload measures are possible. Here, a matrix of twelve measures is proposed which balances theoretical exhaustion with practicality. Using data for one year of adult criminal cases in the Provincial Court of British Columbia it is shown that the choice of measure introduces variability of workload over 400 per cent. The matrix is intended as a heuristic device for policy-makers to scrutinise case-based indicators.Peer reviewedFinal article publishe
Review of Our Rightful Pace: A history of women at the University of Kentucky 1880-1945
No full textBook ReviewPublishe
Synchronization of modified Chua's circuit with x vertical bar x vertical bar function
No full textThis paper considers the chaos synchronization of the modified Chua's circuit with x vertical bar x vertical bar function. We firstly show that a couple of the modified Chua systems with different parameters and initial conditions can be synchronized using active control when the values of parameters both in drive system and response system are known aforehand. Furthermore, based on Lyapunov stability theory we propose an adaptive active control approach to make the states of two identical Chua systems with unknown constant parameters asymptotically synchronized. Moreover the designed controller is independent of those unknown parameters. Numerical simulations are given to validate the proposed synchronization approach
Morphodynamic modeling the impact of large-scale embankment on the large bar in a convergent estuary
No full textMany alluvial estuaries worldwide include an inside bar system, a large sediment deposit deeply stretched into the estuary. A good example of such a system is the large sediment deposit in the Qiantang Estuary, China. Its length and height reach 130 km and 10 m, respectively. Bathymetrical comparison reveals that the large bar has moved seaward by around 15 km over the last decades, probably related to the large-scale coastal embankment project. This motivated a quantitative investigation of the impact of estuarine planform on the inside bar development. The bar morphology is reproduced by means of an idealized 1-D morphodynamic model. Model results suggest that the bar movement is related to a decreasing tidal prism, increasing flood dominance in the lower reach and enhanced ebb currents in the upper reach, in response to the embankment. The timescale of the morphological response is only several years. The rapid response is related to the strong tidal currents and large sediment fluxes within the estuary. Sensitivity experiments show that the location and dimensions of the bar are related to the convergence length of the estuary. A decrease of the convergence length causes seaward movement and shortening and lowering of the bar. The bar dimensions also depend on the ratio between river and tidal discharges. When the ratio increases, the bar apex moves seaward and the elevation decreases. The bar movement has significantly influenced the tidal bore in the Qiantang Estuary.Accepted Author ManuscriptCoastal Engineerin
The Bar-On model of emotional-social intelligence (ESI)1
No full textThe present manuscript is an empirically based theoretical paper that presents, describes, and examines the Bar-On Model of Emotional-Social Intelligence (ESI) in deep. First, a description of the Emotional Quotient Inventory (the EQ-i), which has played an instrumental role in developing the model, is given. The EQ-i is a self-report measure of emotionally and socially intelligent behaviour. It has been translated into more than 30 languages, and data have been collected around the world. The impact of age, gender, and ethnicity on the Bar-On model is presented. A description of the model's construct and predictive validity is given. Finally, the author summarizes the key points, discusses the limitations of the model, and raises the ideas for developing a future model of ESI.El modelo de Bar-On de Inteligencia Emocional-Social. El presente manuscrito es un artículo teórico basado en evidencias empíricas que presenta, describe y examina el modelo de Bar-On de Inteligencia Emocional-Social (IES) en detalle. Comienza proporcionando una descripción del Emotional Quotient Inventory (el EQ-i), el cual ha jugado un papel instrumental en el desarrollo del modelo. El EQ-i es una medida autoinformada del comportamiento emocional y socialmente inteligente. Ha sido traducido a más de 30 lenguas, contando con datos de todo el mundo. La influencia de la edad, el género y la raza sobre el modelo de Bar-On es analizada. Además, se describe la validez de constructo y predictiva de este modelo. Finalmente, el autor resume los puntos clave del modelo, describe sus limitaciones y proporciona algunas ideas para el desarrollo de un futuro modelo de IES
OSB program measures
No full textThis archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Mode of access: Internet from the Oregon Government Publications Collection.Text in English
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