1,721,015 research outputs found
Frequency of the canine leucocyte adhesion deficiency (CLAD) mutation among Irish red setters in Germany
No full textCanine leucocyte adhesion deficiency (CLAD) is an autosomal recessive hereditary disease occurring among Irish red setters. The genetic defect causative for this disorder was recently identified as a missense mutation in the ITGB2 gene. Irish red setters with one copy of the mutant gene appear normal, while dogs with two copies of the mutant gene manifest the disease. The present report describes the analysis of the single nucleotide polymorphism in 289 Irish red setters by DNA sequencing. The frequency of CLAD carriers in Germany is 11%
Molecular genetic possibilities of identification in the dog and comparison with conventional methods
No full textIn human medicine genetic methods are very improtant for identification. We describe in our paper the possibilities of genetic examination in dogs and compare them with conventional techniques of identification
Molecular genetic possibilities of identification in the dog and comparison with conventional methods
No full textIn human medicine genetic methods are very improtant for identification. We describe in our paper the possibilities of genetic examination in dogs and compare them with conventional techniques of identification
X- and Y-chromosome specific variants of the amelogenin gene allow sex determination in sheep (<it>Ovis aries</it>) and European red deer (<it>Cervus elaphus</it>)
No full textAbstract Background Simple and precise methods for sex determination in animals are a pre-requisite for a number of applications in animal production and forensics. However, some of the existing methods depend only on the detection of Y-chromosome specific sequences. Therefore, the abscence of a signal does not necessarily mean that the sample is of female origin, because experimental errors can also lead to negative results. Thus, the detection of Y- and X-chromosome specific sequences is advantageous. Results A novel method for sex identification in mammals (sheep, Ovis aries and European red deer, Cervus elaphus) is described, using a polymerase chain reaction (PCR) and sequencing of a part of the amelogenin gene. A partial sequence of the amelogenin gene of sheep and red deer was obtained, which exists on both X and Y chromosomes with a deletion region on the Y chromosome. With a specific pair of primers a DNA fragment of different length between the male and female mammal was amplified. Conclusion PCR amplification using the amelogenin gene primers is useful in sex identification of samples from sheep and red deer and can be applied to DNA analysis of micro samples with small amounts of DNA such as hair roots as well as bones or embryo biopsies.</p
Phylogenetics of the European Dahomey miniature cattle based on mitochondrial D-loop region DNA sequence
No full textIdentification of new allelic variants in the ovine prion protein (PrP) gene
No full textThe association between scrapie and polymorphisms of the prion protein (PrP) gene was studied in 1108 German sheep of 33 different breeds. The aim of the investigation was the determination of the codons 136, 154 and 171 of the PrP gene, which are important for scrapie susceptibility. In addition to the published allelic variants ARR, ARQ, AHQ, ARH and VRQ, two novel, rare haplotypes (AHR and VRR) were found in the breeds of Texel, Nolana and Suffolk. A comparison of PrP genotype frequencies among the analysed different breeds revealed distinct variations. Breeds such as Texel showed a complex genotype distribution over 17 variants, while breeds such as Friesian Milk Sheep indicated only seven different genotypes
The occurrence of an Australian leech species (genus Helobdella) in German freshwater habitats as revealed by mitochondrial DNA sequences
No full textThe freshwater leech Helobdella europaea Kutschera 1987 was discovered twenty years ago in Germany and described as a new species. Here, we show that this leech is genetically identical with the Australian species Helobdella papillornata (CO-I-mt-DNA sequence identity of alignment positions: 98%). We conclude that H. europaea (syn. H. papillornata) represents an introduced annelid that occupies the same ecological niche as the common European leech H. stagnalis L. (C) 2004 Elsevier Inc. All rights reserved
- …
