2,591 research outputs found
A Collection of Tales by Mr. Ken Sutton
No full textNotes - Mr. Sutton tells eight trapping, hunting and fishing tales, detailing his many exciting encounters with bears, wolves, lynx and storms. He narrates a bad personal accident on a trap line where he severed the main artery to his brain (along with his ear) and lived to tell about it (much to his surgeon's surprise.) His last story tells of a heart attack in 1978 and his road to recovery (26 pages
On morphology, molecular composition and breakdown behaviour in semi-crystalline polymers
No full textMicrobiology Topics. ABOUT THE AUTHOR Measurement of Microbial Cells by Optical Density
No full text"Microbiology Topics" discusses various topics in microbiology of practical use in validation and compliance. We intend this column to be a useful resource for daily work applications. Reader comments, questions, and suggestions are needed to help us fulfill our objective for this column. Please send your comments and suggestions to column coordinator Scott Sutton at scott. [email protected] or journal coordinating editor Susan Haigney at [email protected]. KEY POINTS The following key points are discussed: Quality control (QC) microbiology tests require controlled levels of inocula and require fresh preparations of cells for those inocula The concentration of cells in a suspension can be estimated by optical density, but this must be confirmed by plate count The optical density readings against cell mass are specific to the microorganism species The qualification of these readings must be confirmed after major maintenance to the bench top spectrophotometer (e.g., after replacement of the bulb). There are, of course, two problems with these instructions. The first is that the technician is instructed to use an inoculum of about 10 8 microorganisms per milliliter and then instructed to determine this by plate count. Colony forming units (CFU) and cells (micro-organisms and spores) are different measures. This will inevitably lead to difficulties as the unfortunate lab worker cannot guarantee the number of cells in the suspension, only the number of CFU found. However, we can accept the scientific inaccuracy, as the numbers will generally work out. The more serious problem is the instruction to use the plate count CFU for determination of the inoculum for the test, and that the suspension shall be used immediately. This quite frankly cannot be done. If you use the suspension immediately, the plate counts are unavailable; if you use the plate counts to set the inoculum, then the suspension is at least a day old. DETERMINATION OF INOCULUM FOR THE AET Contrast these instructions with those in the United States Pharmacopeia (USP) (2) for the same exercise: Scott Sutton are needed to help us fulfill our objective fo fo for this column. Please send your comments an an and d d su u ugg gg gges es etions to column coordinator Scott Su Su Sutt tt tton n n at sc sc scot ot ott. t. t. [email protected] or journal co co coor o o dina a nating e e editor Susan Haigney at shaigney@ y@ [email protected]. KEY POINTS suspending fluid … Add sufficient suspend fluid to reduce the microbial count to about micro-organisms per milliliter…Remove imm ately a suitable sample from each suspension d d de d termine the number of colony-forming u per milliliter in each suspension by plate coun membrane filtration (2.6.12). This value se KEY POINTS The following key points are discuss sed ed: Quality control (QC) microbiolog gy test sts s re equ quire controlled levels of inocula and nd r re equi ire f fresh sh preparations of cells for those inocula The co o onc ncen en ntr tr trat at atio io ion n n of cells in n a a su uspen nsi sion o can be es s sti i imated b b by y y op op opti ti tical dens nsity, y, b but ut this s mu must s be membrane filtration (2.6.12). This value se to determine the inoculum and the baselin use in the test. The suspensions shall be u immediately." There are, of course, two problems with these t t tion n ns. s. s. T T The he he f f fir ir irst st i is s s th th hat at t t th h he t t tec e echnicia an is is i ins nstr truc u te an an an ino no noculum of a a abo out 10 0 0 8 8 8 m m mic croorg gan nisms s pe per m c co conf f fir rmed by p p plate te te c c cou o o nt t Th h he op p ptical den n nsit t ty r re rea adin ings gs a agai inst st cel e l m mass a are r s s sp s ec ec ec e ifi i ic to th h h he e e e m m mi m croo o o org gan an anis is sm specie ie es Th Th Th he q qu l l alif if ifi i ication of the h h se readings mu t t st be confirmed e e e a a a after m m m maj j j jor o o mai ai aint t ten e e ance ce ce t t to o o the e e be b b nc c ch h h t t top sp sp sp spec ec e ect t tropho ho ho hoto to to tom m meter r (e (e (e.g g g., aft fter er er r repla ace ement nt nt of the bulb) a a and d d th th then n n i i instru u ucte e ed to o o d d deter erm mine e thi his s by by plat C C Colony f f fo o orm m ming un n nits ( ( (CF C CFU) U) a and nd ce ells s ( (mi mic cro-or an an and d d d sp p por r res es es es) ) ) ) are d d dif f ffer er er ere e en e t measu ur u es es es. T This w w wil ill l l in l l lead d d d t t t to o o di di di dif fficulties as t t the unfortun t t ate lab wo k k rke guar r r ran a a a tee th th th he e e e numb mb mb ber of f f f ce ce ce cell ll lls s in t t the he he s s sus u u pens ns nsi i i the e e nu nu nu numb m m m er o o of CFU U U foun n n nd d. d. d H H Ho ow o ev ver er r, , w w we can n n a a ac scientific inaccuracy as the numbers will genera of the bulb) ) ). DE DE DE DETE TE TE TERM RM RM RMIN IN IN INAT AT AT ATIO IO IO ION N N N OF OF OF OF I I INO NO NOCU CU CULU LU LUM M M FO FO FOR R R scientific inaccuracy, y as the numbers will g genera ou ou ou out. t t t Th Th Th The e e e mo mo mo more re re re s s ser er er erio io io ious us us us p p p pro r ro robl bl bl blem em em i i is s s th th the e e in in inst st stru ru ruct ct ctio o th th th the e e e pl pl pl plat at at a e e e e co co co coun un un unt t t t CF CF CF CFU U U U fo fo fo for r r r de de de dete te te t rm rm rmin in inat at atio io ion n n of of of t t the he he i i in
Using natural experiments to evaluate population health interventions
No full textCraig P, Cooper C, Gunnell D, Haw S, Lawson K, Macintyre S, Ogilvie D, Petticrew M, Reeves B, Sutton M, Thompson
The creative power of language in social cognition and intergroup relations.
No full textThis book chapter reviews the relation of language to thought and its implications for intergroup relations. Following recent advances in social psychology, the chapter argues that language, far from merely being a medium for the transmission of stereotypes and prejudices, has the power to create, augment, and transform them. In particular, I examine the implications of the ability of language to contain thought (like a vessel), to focus thought (like a lens), and to reveal thought (like a barometer)
Mapping B-cell responses to bacterial infection using next generation phage display
No full text<p>Scripts for processing of data for publication. Mapping B-cell responses to bacterial infection using next generation phage display.</p>
<p>Ibrahim A. Naqid, Ben C. Maddison, Jonathan P. Owen, Anastasios Spiliotopoulos, Richard D. Emes, Andrew Warry, Monika A. Tchórzewska, , Francesca Martelli, Rebecca J. Gosling, Robert H. Davies, Roberto M. La Ragione and Kevin C. Gough</p>
<p>School of Veterinary Medicine and Science, The University of Nottingham, Sutton<br>Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD. UK.<br>ADAS UK, School of Veterinary Medicine and Science, The University of Nottingham,<br>Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD. UK<br>Advanced Data Analysis Centre, The University of Nottingham, Sutton Bonington Campus<br>Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey. KT15 3NB. UK.<br>School of Biological Science, Royal Holloway, University of London, Egham, Surrey. TW20 0EX.<br>School of Veterinary Medicine, Daphne Jackson Road, University of Surrey, Guildford, Surrey. GU2 7AL</p>
<p>Corresponding Author: Dr K. C. Gough, School of Veterinary Medicine and Science, The<br>University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington,<br>Leicestershire, LE12 5RD. UK. Telephone: +44-115-9516272; FAX: +44-115-9516440; Email:[email protected]</p>
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Preclinical evaluation of the effect of the combined use of the Ethicon Securestrap® Open Absorbable Strap Fixation Device and Ethicon Physiomesh™ Open Flexible Composite Mesh Device on surgeon stress during ventral hernia repair [Corrigendum]
No full textSutton N, MacDonald MH, Lombard J, et al. [Med Devices (Auckl)]. 2018;11:1–9. On page 1, the co-author’s name was incorrectly listed as Bodgan Ilie. His correct name should be Bogdan Ilie. Read the original articl
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