42,157 research outputs found
Successful treatment of 15 cases of canine traumatic aural hematoma using autologous platelet rich plasma (PRP)
No full textPlatelet-rich plasma (PRP) derived from whole blood, is characterized by platelet (PLT) concentrations above baseline in a small volume of plasma that can accelerate the healing process [1] by providing elevate concentration of platelet-derived growth factors [2] which can stimulate cell proliferation and decrease of inflammatory reaction. Following the excellent results obtained on a small number of subjects previously reported in a preliminary study [3], in this prospective in vivo study we aimed to describe the clinical efficacy of autologous PRP in the treatment of canine traumatic aural hematoma secondary to otitis externa. After approval by the Ethics Committee of the University of Milan and with the owner informed consent, 20 ml of citrate whole blood were obtained from the cephalic vein of 15 dogs with traumatic aural hematoma of different breeds, 6 males and 9 females, with a age range of 1 -15 years (mean ± DS: 7,4 ± 3,7 years). All subjects had a history of multiple centesis, sometimes associated with injections of cortisone in situ, with subsequent recurrences of hematoma. PRP was produced using a semi-automatic closed system (CPUNT 20, Eltek group, Casale Monferrato, Alessandria, Italy) for veterinary use [4]. The serum-hematic content of the auricular pinna was completely drained using one or more 20G needles (depending of hematoma organization) and, using the same hole of the drainage inlet, the PRP was then injected. The dogs were subjected to weekly follow up for a minimum of 45 days from the first treatment. In case of partial or total recurrence of the aural hematoma of the first follow-up, the treatment was repeated with the same procedure. No dog has been subjected to anaesthesia during the procedures. At D0 the aural hematoma was present for 17 ± 13 days and the mean of the drained serum-hematic content was 23 ± 30 ml. Four dogs had a partially organized aural hematoma. 1.3 ± 0.6 ml of PRP were injected, with a mean concentration of 1185 ± 908 x 103/μl PLT (minimum value: 308 x 103/μl PLT maximum value: 4141 x 103/μl PLT, 500% mean increase compared to whole blood). 12/15 subjects were treated with a single application (Group A1), 3/15 with two applications (Group A2). 2/15 subjects were lost after the first follow up. For the remaining 13/15 the mean healing time was 15.8 ± 8.1 days (A1) and 24 ± 5.2 days (A2). No subjects showed recurrences at 45 days follow up. No side effects have been registered. The in situ administration of PRP was effective in the treatment of traumatic aural hematoma secondaty to otitis externa in dogs, leading to complete resolution of the disease in all treated subjects.
[1] Marx R.E. Platelet-Rich Plasma (PRP), what is PRP and what is not PRP? Implant Dentistry 2001, 10, 225-228. [2] Souza T.F. et al. Healing and expression of growth factors (TGF-β and PDGF) in canine radial ostectomy gap containing platelet-rich plasma. Vet Comp Orthop Trauma, 2012, 25, 445-452. [3] Perego R. et al. Efficacia clinica del plasma ricco di piastrine (prp) autologo ottenuto con metodo chiuso semi-automatico nel trattamento dell’otoematoma nel cane. 2017. 56° Congresso AIVPA, Piacenza. [4] Perego R. et al. Evaluation of a commercial closed system for autologous platelet-rich plasma production in dog. 2016. ESVCP-ESVONC Congress, Nantes
The evolution of Unconventional Platforms: applying Platform Thinking in non-digital environments
No full textEfficacy of a Semi Automated Commercial Closed System for Autologous Leukocyte- and Platelet-Rich Plasma (l-prp) Production in Dogs : a Preliminary Study
No full textBackground: To characterize the cellular composition (platelets, erythrocytes, and leukocytes) and determine platelet-derived growth factor isoform BB (PDGF-BB) concentration in canine leukocyte- and platelet rich plasma (L-PRP) produced using a commercial semi-automated closed system. Methods: Twenty milliliters of citrated whole blood were obtained from 30 healthy un-sedated canine blood donors and processed using a semi-automated completely closed commercial system (CPUNT 20, Eltek group, Casale Monferrato, Alessandria, Italy) according to the manufacturer’s instructions. Erythrocyte, leukocyte, and platelet counts were determined in both whole blood (WB) and resultant L-PRP. The PDGF-BB concentration was evaluated after bovine thrombin activation of 10 L-PRP samples. Results: This commercial system produced on average 2.3 ± 0.7 mL of L-PRP containing a high concentration of platelets (767,633 ± 291,001 μL, p < 0.001), with a 4.4 fold increase in platelet count, lower concentration of erythrocytes (528,600 ± 222,773 μL, p < 0.001) and similar concentration of leukocytes (8422 ± 6346 μL, p = 0.9918) compared with WB. L-PRP had an average of 3442 ± 2061 pg/mL of PDGF-BB after thrombin activation. Neutrophils, lymphocytes and monocytes average percent content in L-PRP was 14.8 ± 13.2, 71.7 ± 18.5 and 10.7 ± 6.4, respectively. Conclusion: Sterile canine L-PRP prepared using this semi-automated closed system is easy to obtain, produces a significant increase in platelet count compared to WB and contains a detectable concentration of PDGF-BB after activation. Additional in vitro and in vivo studies are needed to assess inflammatory markers concentration and the therapeutic efficacy of this L-PRP in dogs
Use of Autologous Leucocyte- and Platelet-Rich Plasma (L-PRP) in the Treatment of Aural Hematoma in Dogs
No full textLeukocyte- and platelet-rich plasma (L-PRP) can accelerate the healing process by providing
increased concentrations of platelet-derived growth factors. The objective of this study was to evaluate
the clinical effect of L-PRP in the treatment of canine aural hematomas associated with otitis externa.
Twenty mL of citrated whole blood was collected from each of the 17 dogs included and autologous L-
PRP was produced. The aural hematoma was drained and 0.5–1 mL of L-PRP was injected. The dogs
were examined weekly until 7 days after complete clinical healing. A final clinical follow-up was
performed 6 weeks after the first treatment with L-PRP. If there was recurrence of the aural hematoma
at the first follow-up, the treatment was repeated. In total, 2/17 cases were lost after the first follow-
up. In 5/17 dogs, a short-term recurrence occurred. In 12/15 cases, complete clinical resolution
was achieved with a single L-PRP application (Group A1) and in 3/15 with two treatments (Group
A2). The mean time to complete clinical resolution was 16 ±8.7 days (A1) and 23.3 ±4 days (A2),
respectively. No side effects were reported. The in situ administration of autologous L-PRP resulted
in a complete resolution of the aural hematoma in all dogs that completed the clinical trial
Confronto tra un test immunocromatografico rapido e il test di immunofluorescenza indiretta (IFAT) per la determinazione della positività anticorpale nei confronti di Leishmania infantum nel cane = Comparison of a immunocromatographic test kit with the immunofluorescence antibody test (IFAT) for assaying Leishmania infantum antibodies in dogs
No full textIntroduzione e scopo
- La leishmaniosi canina (LCan) causata da Leishmania infantum è una patologia zoonotica potenzialmente mortale. La disponibilità di un kit diagnostico rapido e facilmente utilizzabile nella pratica clinica rappresenta un valido ausilio per la diagnosi. L’obiettivo di questo studio è di confrontare il test rapido immunocromatografico, Theratest Leishmania ®kit (Tt) con l’immunofluorescenza indiretta (IFAT) quale metodo quantitativo di riferimento, per individuare anticorpi nei confronti di L. infantum, in cani naturalmente infetti.
Materiali e metodi
- Sono stati valutati contemporaneamente con entrambi i test, i sieri di 40 cani, 10 sani e 30 con diagnosi di leishmaniosi canina. Sono
stati considerati positivi titoli IFAT ≥ 1:80. Sono state calcolate sensibilità,
specificità, valore predittivo positivo e negativo del Tt. Per valutare la con-
cordanza tra i due test è stato utilizzato il test statistico Kappa (K) con IC
del 95%.
Risultati
- Il test IFAT ha individuato anticorpi anti-leishmania in 30/40 campioni con titolo anticorpale compreso tra 1:80 e 1:5120. Con il metodo Tt sono risultati positivi 31/40 campioni. Sensibilità e specificità di Theratest sono risultate rispettivamente del 100% e 95%. Il valore Kappa è risultato 0,935 dimostrando ottimo accordo tra metodo IFAT e Tt.
Discussione
- Theratest è in grado di identificare con accuratezza gli anticorpi anti-L. infantum nel siero di cane. Questo test rapido non richiede al-
cuna specifica preparazione dei campioni, né attrezzature dedicate e può essere conservato a temperatura ambiente, caratteristiche che lo rendono adatto ad essere utilizzato nella pratica clinica fornendo risultati in tempi rapidi.Canine leishmaniosis (CanL) due to Leishmania infantum infection is a life-threatening zoonotic disease. The availability of reliable and rapid in-clinic serologic tests, would assist immediate diagnosis in clinically suspected cases
Dextran/poly-L-arginine multi-layered CaCO3-based nanosystem for vascular drug delivery
No full textThe development of heterogeneous drug delivery systems leads to innovative strategies for targeted therapy of common pathologies, such as cancer, immunological and neurological disorders. Nowadays, it is possible to choose among a great variety of nanoparticles on the basis of the needs they have to satisfy. However, a candidate for the treatment of cardiovascular pathologies is still missing. In this context, a targeted therapy implies the conceptualization of nanoparticles that take active part in the treatment of vascular pathologies. The aim of this work was to provide a method to produce multi-layered calcium carbonate (CaCO3) nanoparticles encapsulating a model protein, bovine serum albumin, with model antibodies on their surface. CaCO3 nanoparticles were produced by the combination of complex coacervation and mineralization and were engineered using layer-by-layer technique with a polysaccharide, dextran sulfate, and a homo-poly-amino acid, poly-L-arginine. Morphology, biocompatibility, cellular uptake, influence on cell expression of the inflammatory marker matrix metalloproteinase-9, and hemocompatibility of the nanoparticles were studied. The presence of the dextran/poly-L-arginine layers did not negatively affect the nanoparticle overall characteristics and they did not trigger proinflammatory response in vitro. Taking together all the obtained results, we consider the proposed CaCO3 nanoparticles as a promising tool in cardiovascular field
Evaluation of a commercial closed system for autologous platelet-rich plasma production in dog
No full textBackground
Platelet-rich plasma (PRP) is a product derived from whole blood (WB), characterized by platelet concentrations above baseline in a small volume of plasma. As a result it contains increased concentrations of platelet-derived growth factors.
Objective
To determine platelets, erythrocytes, and leukocytes count and platelet-derived growth factor-BB concentration in the final product of a commercial platelet-rich plasma closed system using canine blood.
Methods
Twenty milliliters of blood was obtained from cephalic vein of 10 healthy blood donors dogs after owner informed consent and processed with a completely closed commercial system CPUNT 20 (Eltek group, Casale Monferrato, Alessandria, Italy) according to the manufacturer’s instructions. Complete blood counts were performed to determine erythrocyte, leukocyte, and platelet numbers in both whole blood and resultant PRP. The PDGF isoform BB concentration was determined after bovine thrombin activation of PRP samples with a human ELISA kit (Human PDGF-BB Duoset DY220E, R&D Systems, Minneapolis, MN, USA) previously used in others canine and feline studies. The normality of data was tested with D’Agostino-Pearson test. Comparison between WB and PRP
parameters was calculated using a paired T-test with significance established at p < 0.05.
Results
This commercial system produced on average 2.73 ± 0.9 ml of PRP containing significant higher concentration of platelets (417 ± 228 x 103/μl, P= 0.003), and lower concentration of erythrocytes (608 ± 217 x 103/μl, P < 0.0001) and leukocytes (7081 ± 3033 /μl, P=0.0003) compared with WB. PRP shows an average of 3442 ± 2061 pg/ml of PDGF-BB after thrombin activation
Structure and macromolecular composition of the zebrafish egg chorion
No full textThe chorion is the acellular envelope surrounding mature eggs of teleostean fish. The macromolecular composition of the zebrafish (Danio rerio) egg chorion, organised as a three-layered structure, has been analysed. SDS-PAGE analysis, under reducing conditions, of isolated and purified chorions revealed a reproducible pattern of four major polypeptides (116, 97, 50 and 43 kDa) and several minor bands. Lectin binding assays showed that both the 116 kDa and 50 kDa proteins were recognised by concanavalin agglutinin (Con A), Galanthus nivalis agglutinin (GNA), Sambucus nigra bark agglutinin (SNA) and Ricinus communis agglutinin (RCA 120), suggesting that these polypeptides are N-linked glycoproteins. By contrast, neither the 97 kDa nor the 43 kDa polypeptides were stained by these lectins, indicating that these polypeptides are not glycosylated. Amino acid analysis also showed significant differences in the average content of some amino acids, for example serine and proline, when compared with previous reports
Hematological reference values for stray colony cats of northern Italy : hematological references intervals for stray cats
No full textIn additional to significant dietary, lifestyle and hormonal differences, stray cats may have different hematological parameters from pet cats. The objectives of this study are to determine hematological reference intervals (RIs) from a large population of stray cats in northern Italy, to establish whether published RIs for the general pet feline population are valid in stray cats and to evaluate the effects of age and sex on hematological parameters. Hematological data were analyzed retrospectively from the database of a trap-neuter-release program (performed in 2008–2010 in northern Italy) to generate normal RIs. RIs were determined and compared with established pet cat RIs according to the National Committee for Clinical Laboratory Standard guidelines and the American Society of Veterinary Clinical Pathology guidelines. Data from 90 healthy stray cats from 17 colonies were available for determination of hematological RIs. Based on the results of comparison with published feline RIs, new RIs were proposed for RBC count, Hct, MCV, MCHC, and WBC count in stray cats. Male cats had a statistically significant higher value than did females for RBC count (mean RBC count in females 6.5 × 1012 versus 7.4 × 1012/L in males, P = 0.001), Hb (mean Hb concentration in females 9.9 versus 10.9 g/dL in males, P = 0.004), and Hct (mean Hct in females 24.8 versus 28.2 % in males, P = 0.001). Significant differences in five hematological parameters were found between stray and pet cats, for most of which the most plausible explanation is probably anesthetic effect and infections or parasitism. It can therefore be assumed that there is no need to establish a specific RI for most of the CBC variables in stray cats
Confronto tra agglutinazione su colonna di gel e su cartina nella determinazione dei gruppi sanguigni nel gatto : risultati preliminari
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