1,721,171 research outputs found
New concepts in the identification and practical application of biocontrol agents against plant pathogens
Soil microclimate could affect the timing of Plasmopara viticola oospores germination and primary infections
The composition of apple and pear bark microbiota suggest microbial migrations from soil
The migration of soil microbial communities possibly define the bark microbiota. The bark microbiota is affected by the bark age and plant specie
Amplicon sequencing and culture-dependent approaches reveal core bacterial endophytes aiding freezing stress tolerance in alpine Rosaceae plants
Wild plants growing in alpine regions are associated with endophytic microbial communities that may support plant growth and survival under cold conditions. The structure and function of endophytic bacterial communities were characterized in flowers, leaves, and roots of three alpine Rosaceae plants in Alpine areas using a combined amplicon sequencing and culture-dependent approaches to determine the role of core taxa on plant freezing stress tolerance. Amplicon sequencing analysis revealed that plant tissue, collection site, and host plant are the main factors affecting the richness, diversity, and taxonomic structure of endophytic bacterial communities in alpine Rosaceae plants. Core endophytic bacterial taxa were identified as 31 amplicon sequence variants highly prevalent across all plant tissues. Psychrotolerant bacterial endophytes belonging to the core taxa of Duganella, Erwinia, Pseudomonas, and Rhizobium genera mitigated freezing stress in strawberry plants, demonstrating the beneficial role of endophytic bacterial communities and their potential use for cold stress mitigation in agriculture.IMPORTANCEFreezing stress is one of the major abiotic stresses affecting fruit production in Rosaceae crops. Current strategies to reduce freezing damage include physical and chemical methods, which have several limitations in terms of costs, efficacy, feasibility, and environmental impacts. The use or manipulation of plant-associated microbial communities was proposed as a promising sustainable approach to alleviate cold stress in crops, but no information is available on the possible mitigation of freezing stress in Rosaceae plants. A combination of amplicon sequencing, culture-dependent, and plant bioassay approaches revealed the beneficial role of the endophytic bacterial communities in alpine Rosaceae plants. In particular, we showed that culturable psychrotolerant bacterial endophytes belonging to the core taxa of Duganella, Erwinia, Pseudomonas, and Rhizobium genera can mitigate freezing stress on strawberry seedlings. Overall, this study demonstrates the potential use of psychrotolerant bacterial endophytes for the development of biostimulants for cold stress mitigation in agricultur
Unravelling the composition of apple and pear bark microbiota through a metabarcoding approach
Characterization of the apple and pear bark microbiota
Bark is considered an important overwintering site for pathogenic, beneficial and saprophytic microorganisms and can act as a reservoir of potential plant pathogens and biocontrol agents. However, the majority of the studies regarding plant-associated microbial communities are focused on the phylloplane, fruits and the rhizosphere, while bark is poorly investigated. The aim of this study was to assess the composition of fungal and bacterial communities of pear and apple barks. Results showed that the amount of cultivable colony forming units (CFUs) and the composition of fungal and bacterial communities differed according to bark age and plant species
Untargeted Metabolomics approach to understand grapevine communication mediated by volatile organic compounds against downy mildew
Volatile organic compounds (VOCs) play crucial roles in the communication of plants with other organisms and are mediators of plant defense against pathogens. The objective of this work is to understand the mechanism of grapevine communications mediated by VOCs against grapevine downy mildew (caused by Plasmopara viticola), using an untargeted metabolomics approach
Development and assessment of plant protein hydrolysates as biopesticides against zucchini powdery mildew
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