1,721,044 research outputs found
Modeling, Testing and manufacturating Illustrated through a Reliable Design of a Biomedical Device Made of Ceramics
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Idrogelo idoneo a contenere e veicolare cellule neuronali
No full textLa presente invenzione riguarda un idrogelo a matrice polimerica interpenetrante biodegradabile ed il suo uso come mezzo per il supporto, l’incapsulamento, la veicolazione ed il rilascio di
diversi tipi di cellule neuronali in condizioni che ne permettono il mantenimento in vita, la crescita, e le interconnessioni. L’invenzione riguarda inoltre l’impiego di detto idrogelo per la
preparazione di impianti per la rigenerazione, ricostruzione e/o sostituzione, intere o parziali, di tessuto neuronale danneggiato, morto o non più funzionale, particolarmente a livello del sistema
nervoso centrale. In tale impiego la biodegradabilità dell’idrogelo permette il rilascio progressivo delle cellule veicolate al fine di favorirne l’integrazione, anche funzionale, con il tessuto
circostante. Inoltre, l’idrogelo oggetto della presente invenzione può essere impiegato per supportare le cellule neuronali su dispositivi di misura appositamente realizzati per monitorare
diversi parametri di attività cellulare, anche durante test di tipo farmacologico
Biological buffered saline solution as solvent in agar-carbomer hydrogel synthesis
No full textAbstractThe role of phosphate buffer saline solution (PBS) was investigated here as a solvent in the polycondensation synthesis of an injectable agar-carbomer based hydrogel, a promising new material specifically intended for regenerative medicine applications. The effects of PBS, with respect to standard distilled water (DW), were quantitatively assessed. Experiments were performed both from physico-chemical and biological points of view. Titration showed higher stability due to the presence of the buffer solution; ESEM analysis confirmed its distribution along the polymeric fibers and infrared spectroscopy showed the consequent anionic nature of the polymeric network. This electrostatic nature of the matrix was confirmed by mass equilibrium swelling data performed at different pH values of the swelling medium. A very relevant role of the solvent was observed also with respect to cell housing inside such hydrogels: living cell counts showed a high amount of cells surviving the latency period of encapsulation in hydrogel when PBS was applied while only very few survived in a deionized water based gel. Obtained data allowed a novel understanding of the causeeffect cascades of all observed phenomena which suggest the PBS fundamental role both in fine control of hydrogel preparation and in material tuning according to the specific needs of different target tissues; the latter being a feature of primary importance when applying hydrogels as cell carriers in regenerative medicine applications.</jats:p
A Library of Tunable Agarose Carbomer-Based Hydrogels for Tissue Engineering Applications: The Role of Cross-Linkers
No full textChemical engineering approach to regenerative medicine
No full textAbstractThe intrinsically multi-factorial pathological trend of spinal cord injury is probably the most important reason behind the absence of efficient therapeutic strategies. Therefore, recent studies suggest the use of new tools combining the delivery of both cells and drugs. Systems which are able to perform multiple controlled delivery of different therapeutic agents have gained particularly strong interest. Hence, in order to avoid trial and error approaches, several studies were performed following the classic chemical engineering multiscale approach: tuning microchemistry to manipulate macro properties in order to satisfy specific medical needs as injectability, low stress on target tissues, ability to retain liquids, capability of carrying living cells, and possibility to control the delivery of drugs. In this framework we focused on injectable agarose-carbomer based hydrogels applying he results of our studies performed in the past two years: in vitro biocompatibility, physical chemical studies, drug delivery transport phenomena investigation, and in vivo biocompatibility in uninjured Brainbow mice.</jats:p
Bovine bone matrix/poly(l-lactic-co-ε-caprolactone)/gelatin hybrid scaffold (SmartBone®) for maxillary sinus augmentation: A histologic study on bone regeneration
Full text linkThe ideal scaffold for bone regeneration is required to be highly porous, non-immunogenic, biostable until the new tissue formation, bioresorbable and osteoconductive. This study aimed at investigating the process of new bone formation in patients treated with granular SmartBone® for sinus augmentation, providing an extensive histologic analysis. Five biopsies were collected at 4-9 months post SmartBone® implantation and processed for histochemistry and immunohistochemistry. Histomorphometric analysis was performed. Bone-particle conductivity index (BPCi) was used to assess SmartBone® osteoconductivity.At 4 months, SmartBone® (12%) and new bone (43.9%) were both present and surrounded by vascularized connective tissue (37.2%). New bone was grown on SmartBone® (BPCi=0.22). At 6 months, SmartBone® was almost completely resorbed (0.5%) and new bone was massively present (80.8%). At 7 and 9 months, new bone accounted for a large volume fraction (79.3% and 67.4%, respectively) and SmartBone® was resorbed (0.5% and 0%, respectively). Well-oriented lamellae and bone scars, typical of mature bone, were observed. In all the biopsies, bone matrix biomolecules and active osteoblasts were visible. The absence of inflammatory cells confirmed SmartBone® biocompatibility and non-immunogenicity. These data indicate that SmartBone® is osteoconductive, promotes fast bone regeneration, leading to mature bone formation in about 7 months
IDROGELO IDONEO A CONTENERE E VEICOLARE CELLULE
No full textLa presente invenzione riguarda un idrogelo a matrice polimerica
interpenetrante biodegradabile ed il suo uso come mezzo per il supporto, l’incapsulamento, la veicolazione ed il rilascio di diversi tipi di cellule in condizioni che ne permettono il mantenimento in vita, la crescita, e le interconnessioni. L’invenzione riguarda inoltre l’impiego di detto idrogelo per la preparazione di impianti per la rigenerazione, ricostruzione e/o sostituzione, intere o parziali, di tessuti danneggiati, morti o non più funzionali, per esempio a livello muscolare, del miocardio, del tessuto cartilagineo, osseo, tendineo, del fegato, del derma, dell’epidermide, del tessuto corneale e del tessuto connettivale in generale. In tale impiego la biodegradabilità dell’idrogelo permette il rilascio progressivo delle cellule veicolate al fine di favorirne l’integrazione, anche funzionale, con il tessuto
circostante.
Inoltre, l’idrogelo oggetto della presente invenzione può essere
impiegato anche per supportare le cellule su dispositivi di misura
appositamente realizzati per monitorare diversi parametri di attività cellulare, anche durante test di tipo farmacologico o biomeccanico
A new model of resorbable device degradation and drug release - part I: zero order model
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