187,032 research outputs found
Survival analysis of the influence of clinicopathological variables including the preoperative endocrine prognostic index (PEPI) and PEPI combined with the primary PgR status (PEPI-P).
No full textSurvival analysis of the influence of clinicopathological variables including the preoperative endocrine prognostic index (PEPI) and PEPI combined with the primary PgR status (PEPI-P).</p
Cumulative survival of patients with breast cancer stratified by our modified PEPI including the primary PgR status (PEPI-P).
No full textRelationships of PEPI-P with (A) recurrence-free (RFS) and (B) cancer-specific survival (CSS).</p
Cumulative survival of patients with breast cancer stratified by the original preoperative endocrine prognostic index (PEPI).
No full text(A) Recurrence-free survival (RFS) was significantly worse in the high PEPI group than in the low PEPI group (P = 0.013). (B) No significant differences were noted in cancer-specific survival (CSS) between the high and low PEPI groups (P = 0.092).</p
GAS-PHASE CHEMISTRY OF DIPHOSPHATE ANIONS AS A TOOL TO INVESTIGATE THE INTRINSIC REQUIREMENTS OF PHOSPHATE ESTER ENZYMATIC REACTIONS: THE [M1M2HP2O7]- IONS
No full textExperimental studies on gaseous inorganic phosphate ions are practically nonexistent, yet they can prove helpful for a better understanding of the mechanisms of phosphate ester enzymatic processes. The present contribution extends our previous investigations on the gas-phase ion chemistry of diphosphate species to the [M1M2HP2O7]- ions where M1 and M2 are the same or different and correspond to the Li, Na, K, Cs, and Rb cations.
The diphosphate ions are formed by electrospray ionization of 10-4M solutions of Na5P3O10 in CH3CN/H2O (1/1) and MOH bases or M salts as a source of M+ cations. The joint application of mass spectrometric techniques and quantum-mechanical calculations makes it possible to characterize the gaseous [M1M2HP2O7]- ions as a mixed ionic population formed by two isomeric species: linear diphosphate anion coordinated to two M+ cations (group I) and[PO3···MH2PO4]- clusters II and the [PO3···M1M2···HPO4]- clusters (group II). The relative gas-phase stabilities and activation barriers for the isomerization I-> II, which depend on the nature of the M+ cations, highlight the electronic
susceptibility of P-O-P bond breaking in the active site of enzymes. The previously unexplored gas-phase reactivity of [M1M2HP2O7]- ions towards alcohols of different acidity was investigated by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR/MS). The reaction proceeds by addition of the alcohol molecule followed by elimination of a water molecule
Iacopo da Benevento, giudice e poeta alla corte di Federico II: “status quaestionis”
No full textL’intervento si concentra sui problemi identificativi e cronologici riguardanti la figura storica e letteraria di Iacopo da Benevento, giudice e poeta vissuto in epoca federiciana, autore della commedia elegiaca «De uxore cerdonis» e dei «Carmina moralia», troppo spesso confuso con un altro Iacopo da Benevento, a lui contemporaneo, un frate domenicano autore del «Viridarium consolationis» e di altri scritti di carattere omiletico ed esegetico.The intervention focuses on the identification and chronological problems concerning the historical and literary figure of Iacopo from Benevento, judge and poet who lived in the Frederick era, author of the elegiac comedy «De uxore cerdonis» and of the «Carmina moralia», too often confused with another Iacopo from Benevento, a contemporary of him, a Dominican friar, author of the «Viridarium consolationis» and other writings of a homiletic and exegetical nature
L’Illuminismo siciliano e le sue frontiere nell’opera del giurista Antonino Pepi
No full textIl contributo esamina l'Illuminismo siciliano attraversol'opera del giurista Antonino Pepi
Impact of combining the progesterone receptor and preoperative endocrine prognostic index (PEPI) as a prognostic factor after neoadjuvant endocrine therapy using aromatase inhibitors in postmenopausal ER positive and HER2 negative breast cancer
No full textThe preoperative endocrine prognostic index (PEPI) predicts survival after neoadjuvant endocrine therapy (NAE) using aromatase inhibitors (AIs) for women with postmenopausal estrogen receptor (ER)-positive breast cancer irrespective of the human epidermal growth factor receptor 2 (HER2) status. Although the progesterone receptor (PgR) is also a prognostic factor for ER-positive breast cancer, the PgR status was not considered a prognostic factor in the original PEPI scoring system. In this study, we investigated the utility of a modified PEPI including the PgR status (PEPI-P) as a prognostic factor after NAE for postmenopausal patients with ER-positive and HER2-negative breast cancer. We enrolled 107 patients with invasive ER-positive and HER2-negative breast cancer treated with exemestane for ≥4 months as NAE. We initially assessed PEPI and compared survival between the groups. Additionally, we obtained an effective cutoff for PgR through survival analysis. Then, we assessed the survival significance of PEPI-P. A PgR staining rate of 50% was the most significant cutoff for predicting recurrence-free survival (RFS) and cancer-specific survival (CSS). PEPI was a significant prognostic factor; moreover, PEPI-P was the most significant prognostic indicator for RFS and CSS. PEPI-P is a potent prognostic indicator of survival after NAE using AIs for postmenopausal patients with ER-positive and HER2-negative breast cancer. This modified PEPI may be useful for therapeutic decision-making regarding postmenopausal ER-positive and HER2-negative breast cancer after NAE.</div
New insights on myocardial pyridine nucleotides and thiol redox state in ischemia and reperfusion damage
No full textOBJECTIVE: to investigate the changes of pyridine nucleotides and thiol redox state in cardiac tissue following ischemia and reperfusion. NADH/NAD and NADPH/NADP redox couples were specifically studied and the influence of NADPH availability on cellular thiol redox was also investigated. METHODS: isolated rabbit hearts were Langendorff perfused and subjected to a protocol of ischemia and reperfusion. An improved technique for extraction and selective quantitation of pyridine nucleotides was applied. RESULTS: ischemia and reperfusion induced an increase in diastolic pressure, limited recovery in developed pressure and loss of creatine phosphokinase. Creatine phosphate and ATP were decreased by ischemia and only partially recovered during reperfusion. NADH was increased (from 0. 36+/-0.04 to 1.96+/-0.15 micromol/g dry wt. in ischemia, P<0.001), whereas NADPH decreased during ischemia (from 0.78+/-0.04 to 0. 50+/-0.06 micromol/g dry wt., P<0.01) and reperfusion (0.45+/-0.03 micromol/g dry wt.). Furthermore, we observed: (a) release of reduced (GSH) and oxidised glutathione (GSSG) during reperfusion; (b) decreased content of reduced sulfhydryl groups during ischemia and reperfusion (GSH: from 10.02+/-0.76 to 7.11+/-0.81 nmol/mg protein, P<0.05, and to 5.48+/-0.57 nmol/mg protein; protein-SH: from 280.42+/-12.16 to 135.11+/-17.00 nmol/mg protein, P<0.001, and to 190.21+/-11.98 nmol/mg protein); (c) increased content in GSSG during reperfusion (from 0.17+/-0.02 to 0.36+/-0.02 nmol/mg protein, P<0.001); (d) increased content in mixed disulphides during ischemia (from 6.14+/-0.13 to 8.31+/-0.44 nmol/mg protein, P<0.01) and reperfusion (to 9.87+/-0.82 nmol/mg protein, P<0.01). CONCLUSIONS: under severe low-flow ischemia, myocardial NADPH levels can decrease despite the accumulation of NADH. The reduced myocardial capacity to maintain NADPH/NADP redox potential can result in thiol redox state changes. These abnormalities may have important consequences on cellular function and viability
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