2,797 research outputs found
Author Correction: Structural basis for translational control by the human 48S initiation complex
No full textMolecular and phylogenetic characterization of honey bee viruses, Nosema microsporidia, protozoan parasites, and parasitic mites in China
No full textChina has the largest number of managed honey bee colonies, which produce the highest quantity of honey and royal jelly in the world; however, the presence of honey bee pathogens and parasites has never been rigorously identified in Chinese apiaries. We thus conducted a molecular survey of honey bee RNA viruses, Nosema microsporidia, protozoan parasites, and tracheal mites associated with nonnative Apis mellifera ligustica and native Apis cerana cerana colonies in China. We found the presence of black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV), Israeli acute paralysis virus (IAPV), and sacbrood virus (SBV), but not that of acute bee paralysis virus (ABPV) or Kashmir bee virus (KBV). DWV was the most prevalent in the tested samples. Phylogenies of Chinese viral isolates demonstrated that genetically heterogeneous populations of BQCV, CBPV, DWV, and A. cerana-infecting SBV, and relatively homogenous populations of IAPV and A. meliifera-infecting new strain of SBV with single origins, are spread in Chinese apiaries. Similar to previous observations in many countries, Nosema ceranae, but not Nosema apis, was prevalent in the tested samples. Crithidia mellificae, but not Apicystis bombi was found in five samples, including one A. c. cerana colony, demonstrating that C. mellificae is capable of infecting multiple honey bee species. Based on kinetoplast-encoded cytochrome b sequences, the C. mellificae isolate from A. c. cerana represents a novel haplotype with 19 nucleotide differences from the Chinese and Japanese isolates from A. m. ligustica. This suggests that A. c. cerana is the native host for this specific haplotype. The tracheal mite, Acarapis woodi, was detected in one A. m. ligustica colony. Our results demonstrate that honey bee RNA viruses, N. ceranae, C. mellificae, and tracheal mites are present in Chinese apiaries, and some might be originated from native Asian honey bees
An Introduction to Zen Buddhism
No full textOne of the world's leading authorities on Zen Buddhism, D. T. Suzuki was the author of more than a hundred works on the subject in both Japanese and English, and was most instrumental in bringing the teachings of Zen Buddhism to the attention of the Western world. Written in a lively, accessible, and straightforward manner, An Introduction to Zen Buddhism is illuminating for the serious student and layperson alike. Suzuki provides a complete vision of Zen, which emphasizes self-understanding and enlightenment through many systems of philosophy, psychology, and ethics. With a foreword by the renowned psychiatrist Dr. Carl Jung, this volume has been generally acknowledged a classic introduction to the subject for many years. It provides, along with Suzuki's Essays and Manual of Zen Buddhism, a framework for living a balanced and fulfilled existence through Zen.Cover Page -- Title Page -- Copyright Page -- Contents -- Preface -- Foreword -- I Preliminary -- II What Is Zen? -- III Is Zen Nihilistic? -- IV Illogical Zen -- V Zen a Higher Affirmation -- VI Practical Zen -- VII Satori, or Acquiring a New Viewpoint -- VIII The Koan -- IX The Meditation Hall and the Monk's Life -- Footnotes -- Foreword Footnotes -- Chapter I Footnotes -- Chapter II Footnotes -- Chapter III Footnotes -- Chapter IV Footnotes -- Chapter V Footnotes -- Chapter VI Footnotes -- Chapter VII Footnotes -- Chapter VIII Footnotes -- Chapter IX FootnotesOne of the world's leading authorities on Zen Buddhism, D. T. Suzuki was the author of more than a hundred works on the subject in both Japanese and English, and was most instrumental in bringing the teachings of Zen Buddhism to the attention of the Western world. Written in a lively, accessible, and straightforward manner, An Introduction to Zen Buddhism is illuminating for the serious student and layperson alike. Suzuki provides a complete vision of Zen, which emphasizes self-understanding and enlightenment through many systems of philosophy, psychology, and ethics. With a foreword by the renowned psychiatrist Dr. Carl Jung, this volume has been generally acknowledged a classic introduction to the subject for many years. It provides, along with Suzuki's Essays and Manual of Zen Buddhism, a framework for living a balanced and fulfilled existence through Zen.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
City-zen: New Urban Energy: Sevilla 'City-zen Roadshow' REPORT
No full textThe City-zen Roadshow travels with a team of internationally recognized experts, in the field of energy planning and design to help develop a sustainable agenda for cities and their neighbourhoods. The Roadshow team work closely with people from the hosting city, whether they be city leaders, energy planners, local architects, professionals, academics, students and of course the citizens themselves. The Roadshow spends 5 days in each hosting city to deliver energy and urban design workshops in which all local stakeholders are welcome and encouraged to join and to take ownership of the final outcomes. Outcomes that will allow the cities recourses, both people and energy, to be directed effectively, by highlighting the energy challenges and potentials to be found in their neighbourhoods, and to finally present a sustainable ‘City Vision’.The following report will describe the activities and outcomes of the City-zen ‘Sevilla’ Roadshow that took place in Sevilla, between the 20th & 24th of November 2017.Acknowledgements: The Sevilla Roadshow would not have been possible without the commitments and organisational energy of one individual: Prof. Madelyn Marrero Melendez, highly respected member of the Escuela Tecnica Superior de Ingeniería de Edificación (Universidad de Sevilla). Madelyn and her team of sustainability educators gave continuous support during the preparations for the Sevilla SWAT Studio and later Roadshow. The Roadshow team would like to take this opportunity to thank Madelyn and her committed colleagues at the Universidad de Sevilla. We wish them well in their continued efforts to make Sevilla a zero-energy city, and wish them every success in taking the outcomes of the City-zen ‘Sevilla’ Roadshow to the next level of realization. The Ingeniería de Edificación would be the home of the SWAT Studio and Roadshow during our co-creative efforts to develop a Sustainable City Vision.Climate Design and Sustainabilit
City-zen: New Urban Energy: Izmir 'City-zen Roadshow' REPORT
No full textThe City-zen Roadshow travels with a team of internationally recognized experts, in the field of energyplanning and design to help develop a sustainable agenda for cities and their neighbourhoods. It will visit 9 cities in total over a 5-year period who are seeking expert guidance on how to become more sustainable and wish to move towards energy neutrality. The overall aim of the Roadshow team, known as ‘Roadies’, is to work closely with people from the hosting city, whether they be city leaders, energy planners, local architects, professionals, academics, students and of course the citizens themselves. The Roadshow spends 5 days in each hosting city to deliver energy and urban design workshops in which all local stakeholders are welcome and encouraged to join and to take ownership of the final outcomes. Outcomes that will allow the cities recourses, both people and energy, to be directed effectively, by highlighting the energy challenges and potentials to be found in their neighbourhoods, and to finally present a sustainable ‘City Vision’.The following report will describe the activities and outcomes of the Roadshow that took place in Bornova, a municipality of the city of Izmir (Turkey) between the 4th & 8th of April 2016.The City-zen 'Izmir' Report would be published as part of Bornova's Municipality's 'Ideas for Bornova' document. Bornova is a district of Izmir.Climate Design and Sustainabilit
Structural basis for translational control by the human 48S initiation complex
No full textAbstract The selection of an open reading frame (ORF) for translation of eukaryotic mRNA relies on remodeling of the scanning 48S initiation complex into an elongation-ready 80S ribosome. Using cryo-electron microscopy, we visualize the key commitment steps orchestrating 48S remodeling in humans. The mRNA Kozak sequence facilitates mRNA scanning in the 48S open state and stabilizes the 48S closed state by organizing the contacts of eukaryotic initiation factors (eIFs) and ribosomal proteins and by reconfiguring mRNA structure. GTPase-triggered large-scale fluctuations of 48S-bound eIF2 facilitate eIF5B recruitment, transfer of initiator tRNA from eIF2 to eIF5B and the release of eIF5 and eIF2. The 48S-bound multisubunit eIF3 complex controls ribosomal subunit joining by coupling eIF exchange to gradual displacement of the eIF3c N-terminal domain from the intersubunit interface. These findings reveal the structural mechanism of ORF selection in human cells and explain how eIF3 could function in the context of the 80S ribosome
Experiential learning: An exploration of the effect of Zen experience on personal transformation
No full textThis thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.This inquiry started by examining my own and others experience of Zen, and comparing it with Self Organised Learning. The aim was to see what effect each system had on the lives of the participants. The thesis plots how I had a tacit reliance on myself as a measuring instrument, and how this became an integrating theme running through my 'finally chosen' methods. The methodological difficulties caused by the paradox of trying to understand Zen and also be scientific converged when I realised that I had treated myself as the central measuring instrument throughout the inquiry. It was this discovery which allowed the thesis to be treated as a koan from a Zen perspective and yet to be a contribution to academic knowledge. The thesis traces how personal authenticity became the defining characteristic informing all my methodology.
This inquiry asks and answers the question can research be transpersonal? Initially the research started out looking at a transpersonal issue in the form of asking those who had regular interactions with a Zen master about their experience. This learning curve was contrasted with Learning Conversations with postgraduates at the centre for the Study of Human Learning, using inner directed learning in their research projects. During the research process, several major re-orientations took place which, necessitated changing my method and my interpretation of the data. These shifts of direction were largely driven by a need to find a method of inquiry which was appropriate to uncovering the transpersonal qualities I was investigating. As the inquiry developed I widened my sources of data to include art, fiction, accounts of death and grieving, and satsang (questions and answers with a master) in order to give an in depth picture of the impact of the transpersonal on participants' lives.
In treating the thesis as a koan there can be no emphasis placed on which purposes related to which outcomes. It was in the gradual abandonment of such a stance that the deeper insights and resolutions occurred. During the inquiry I eventually identified the qualities of wholeness, authenticity and openness as the defining characteristics which appeared to trigger changes in direction. Such an approach made it necessary to examine the implications for validity that approaching transpersonal issues in this way had uncovered
City-zen: New Urban Energy: Roeselare 'City-zen Roadshow' REPORT
No full textThe City-zen Roadshow travels with a team of internationally recognized experts, in the field of energy planning and design to help develop a sustainable agenda for cities and their neighbourhoods. It will visit 9 cities in total over a 5-year period who are seeking expert guidance on how to become more sustainable and wish to move towards energy neutrality. The overall aim of the Roadshow team is to work closely with people from the hosting city, whether they be city leaders, energy planners, local architect, professionals, academics, students and citizens. The Roadshow spends 5 days in each hosting city to deliver energy and urban design fun-shops in which all local stakeholders are welcome and encouraged to join and to take ownership of the final outcomes. Outcomes that will allow the cities recourses, both people and energy, to be directed effectively, by highlighting the energy challenges and potentials to be found in their neighbourhoods, and to finally present a sustainable ‘City Vision’. The following report will describe the activities and outcomes of the Roeselare Roadshow that took place in Roeselare (Belgium), between the 23rd & 27th of April 2018.The Roeselare Roadshow would not have been possible without the commitments and organisational energy of one individual. That person, Timo Wyffels, highly respected member of the Municipality of Roeselare, Energie en Duurzaamheid bij Stad Roeselare. Timo and his team of sustainability experts from the Klimaatswitch team gave continuous support during the preparations for the Roeselare SWAT Studio and later Roadshow. The Roadshow team would like to take this opportunity to thank Timo and his committed colleagues at the Municipality. We wish them well in their continued efforts to make Roeselare a zero-energy city, and wish them every success in taking the outcomes of the City-zen Roeselare Roadshow to the next level of realization. Special mentions must also go to the Mayor of Roeselare, Kris Declercq, whose hospitality, encouragement and enthusiasm brought the Roadshow in meaningful partnership with the city. The day to day organisation and ‘engine room’ of the SWAT Studio and the Roadshow would be powered by the tenacity, amenability and zeal of one young member of the Klimaatswitch team, Janne Ruszkowski. We thank Kris and Janne for helping to make the Roadshow an impactful success. The Huis van de Voeding (House of Food) in the city centre of Roeselare would be the home of the SWAT Studio and Roadshow during our co-creative efforts to develop a sustainable City Vision. We’d like to thank organises for the hospitality they showed to the SWAT and Roadshow team during our extended stay there. The activities and energy within the House of Food gave even greater impetus to the Roadshow team and their stakeholder partners to go to zero carbon.Climate Design and Sustainabilit
Native tradition in korean zen
No full textMu Soeng Sunim is senior monk and Abbot of the Diamond Hill Zen Monastery in Cumberland, Rhode Island. He is the author of "Thousand Peaks", a history of Korean Zen. (Part one of a talk given at the Providence Zen Center in January, 1987
Active role of elongation factor G in maintaining the mRNA reading frame during translation.
No full textDuring translation, the ribosome moves along the mRNA one codon at a time with the help of elongation factor G (EF-G). Spontaneous changes in the translational reading frame are extremely rare, yet how the precise triplet-wise step is maintained is not clear. Here, we show that the ribosome is prone to spontaneous frameshifting on mRNA slippery sequences, whereas EF-G restricts frameshifting. EF-G helps to maintain the mRNA reading frame by guiding the A-site transfer RNA during translocation due to specific interactions with the tip of EF-G domain 4. Furthermore, EF-G accelerates ribosome rearrangements that restore the ribosome's control over the codon-anticodon interaction at the end of the movement. Our data explain how the mRNA reading frame is maintained during translation
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