1,721,131 research outputs found
Evaluation of assay procedures for prediction of passive transfer status in lambs
No full textObjective-To compare 4 assay procedures for prediction of passive transfer status in lambs. Animals-Thirty-one 1-day-old Sardinian lambs. Procedure-Serum IgG concentration was determined by use of single radial immunodiffusion, The following were determined: serum total protein concentration as measured by refractometry (ie, refractometry serum total protein concentration), serum total protein concentration as determined by the biuret method (ie, biuret method serum total protein concentration), serum gamma-globulin concentration as determined by serum protein electrophoresis, and serum gamma-glutamyltransferase (GGT) activity as measured by spectrophotometry. Accuracy of these assays for estimation of serum IgG concentration in 1-day-old lambs was established by use of linear regression analysis. Results-Refractometry serum total protein concentration, biuret method serum total protein concentration, and serum gamma-globulin concentration were closely and linearly correlated with serum IgG concentration. The natural logarithm (In) of serum GGT activity was closely and linearly correlated with serum IgG concentration (In) Refractometry serum total protein concentration, biuret method serum total protein concentration, and gamma-globulin concentration accounted for approximately 85%, 91%, and 95% of the variation in serum IgG concentration, respectively. Serum GGT activity (In) accounted for approximately 92% of the variation in serum IgG concentration (In). Conclusions and Clinical Relevance-For prediction of passive transfer status in 1-day-old lambs, serum GGT activity or biuret method serum total protein concentration determination will allow for passive transfer monitoring program development. Immediate refractometry serum total protein concentration determination is beneficial in making timely management and treatment decisions. Serum gamma-globulin concentration determination can be used as a confirmatory test.[...
Valutazione della risposta immunitaria in soggetti persistentemente infetti da BVDV mediante quantificazione citochinica
No full textLa Diarrea Virale Bovina è una patologia infettiva responsabile di gravi danni nell’allevamento bovino. Nonostante la malattia sia ben conosciuta e studiata da molti decenni ancora non è possibile controllare efficacemente né l’effetto immodepressivo, né l’infezione congenita che conseguono all’infezione del suo agente causale (virus della diarrea virale bovina - BVDV). Questa situazione è prevalentemente dovuta alle carenti conoscenze che si hanno sull’effetto dell’infezione virale sul sistema immunitario dell’ospite. L’obiettivo della nostra indagine è stato pertanto quello di valutare lo stato d’immunodepressione in 12 soggetti con infezione naturale da BVDV. A tal fine è stata comparata l’espressione genica delle citochine interleuchina 8 (IL-8) e interferone gamma (IFN-γ) misurata, tramite real time PCR in diversi organi linfoidi provenienti da soggetti infetti e sani. L’espressione genica è stata poi direttamente correlata alla presenza del virus quantificato tramite PCR quantitativa. L’indagine ha mostrato più bassi valori di RNA messaggero dei mediatori ricercati nei campioni derivanti dai soggetti infetti. Inoltre è stata evidenziata una più bassa espressione citochinica negli organi com maggior quantità di virus anche se tale correlazione non ha trovato conforto statistico. Gli Autori discutono delle possibili ragioni di tale riscontro. dimostrando una minore capacità reattiva della risposta immunitaria cellulo-mediata e dell’attività chemiotattica nei soggetti infetti con BVDV
Evaluation of serum enzyme activities as predictors of passive transfer status in lambs
No full textObjective-To determine the associations between serum IgG concentration and serum activities of gamma-glutamyltransferase (GGT), alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, and pseudocholinesterase for the potential use of these serum enzymes as predictors of passive transfer status in neonatal lambs. Design-Prospective observational study. Animals-47 Sardinian lambs from birth to 2 days old. Procedure-Serum enzyme activities were measured by use of commercially available kits and a clinical biochemical analyzer. Serum IgG concentration was determined by single radial immunodiffusion. Associations between serum IgG concentration and the activity of each serum enzyme were established by use of regression analysis. Results-A significant correlation was detected between serum IgG concentration and serum GGT activity in 1- and 2-day-old lambs. Minimal correlations were detected between serum IgG concentration and serum alkaline phosphatase activity in 1-day-old lambs and serum pseudocholinesterase activity in 1- and 2-day-old lambs. No significant associations were detected between serum IgG concentration and serum activities of aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase. A multiple linear regression model was accurate for the estimation of the natural logarithm of serum IgG concentration as a function of the natural logarithm of serum GGT activity and of the age of lambs at the time of sampling (adjusted R-2 = 0.89). This model was then used to calculate the serum GGT activity equivalent to various serum IgG concentrations for 1- and 2-day-old lambs. Conclusions and Clinical Relevance-Results suggested that passive transfer status in neonatal lambs can be successfully predicted by measurement of serum GGT activity but not by measurement of the other enzymes tested.[...
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