257 research outputs found
Development and evaluation of smartphone-based ITS applications for vehicular networks
No full text[ES] Una de las áreas de investigación que está recibiendo más atención recientemente es la de vehículos autónomos. Los investigadores están en este momento centrados en el tercer de los cinco niveles de autonomía, los cuales son: asistencia en la conducción, automatización parcial, automatización condicional, alta automatización y automatización completa. A pesar de los rápidos progresos que están habiendo en este campo, la adopción de estas soluciones llevará tiempo no sólo debido a cuestiones legales, sino también por el hecho de que los avances tecnológicos se enfrentan a un lento respaldo por parte de los fabricantes. Además, la baja tasa de renovación de vehículos de carretera, dificulta el despliegue de tecnologías innovadoras, como es el caso de la red vehicular. Ocho años después de la introducción de la norma 802.11p para la comunicación vehicular del Instituto de Ingenieros Eléctricos y Electrónicos (IIEE), los vehículos que se usan a diario todavía carecen de la capacidad de comunicarse entre sí. Este hecho impide el uso de las muchas aplicaciones de seguridad del Sistema de Inteligencia de Transporte (SIT) que aprovecha la red vehicular para el intercambio de datos. La forma obvia de manejar este problema es poner las tecnologías disponibles a la disposición de los usuarios comunes para desarrollar soluciones que se puedan implementar fácilmente y, además, económicas.
Por esta razón, trasladamos nuestra atención a los dispositivos inteligentes, especialmente a los teléfonos inteligentes, los cuales han recorrido un largo camino desde la primera introducción de teléfonos móviles a finales del siglo XX. Hoy en día casi todos llevan uno en su bolsillo a donde sea que vayan, permitiéndoles no sólo hacer llamadas, sino también medir y controlar diferentes parámetros con la ayuda de los muchos sensores integrados que están disponibles para estos dispositivos compactos pero potentes. Nuestro objetivo es estudiar los efectos de la integración de los teléfonos inteligentes a la red vehicular para desarrollar aplicaciones de seguridad del SIT. La elección de los teléfonos inteligentes aquí no solo está justificada por su amplia disponibilidad y uso, sino también porque están evolucionando hacia terminales de alto rendimiento con microprocesadores de múltiples núcleos cargados dotados de un grupo suficientemente diverso de sensores. En esta tesis proponemos tres diferentes aplicaciones de seguridad SIT para teléfonos inteligentes, diseñados para aprovechar el entorno de red vehicular: una aplicación de generación de advertencia llamada Messiah que alerta a los conductores de la presencia de vehículos de emergencia en las cercanías; una aplicación de Advertencia de Colisión Frontal (ACF) que advierte a los conductores si no se mantiene la distancia de seguridad mínima entre el vehículo que va delante y el que lo sigue; y, por último, una aplicación que tiene como objetivo ayudar a los conductores con asistencia visual durante el adelantamiento, llamada EYES. Todas estas aplicaciones han sido desarrolladas para la plataforma Android, y dependen de la transmisión de datos entre vehículos. Dado que los vehículos que utilizamos día a día no admiten la posibilidad de comunicarse entre sí, también diseñamos GRCBox, que es una unidad integrada de bajo coste que permite la comunicación del Vehículo a Todo (V2X).
A partir de nuestro estudio de aplicaciones para dispositivos móviles diseñados para redes vehiculares, descubrimos que el uso de teléfonos inteligentes proporciona una nueva dirección para la investigación relacionada con SIT y redes vehiculares al permitir la adopción rápida de las soluciones existentes, donde los usuarios pueden descargar y usar las aplicaciones con sólo un clic a un botón. Al mismo tiempo, la portabilidad y compacidad de los dispositivos los hace limitados en términos de velocidad, potencia de procesamiento y precisi[CA] Una de les àrees d'investigació que està rebent més atenció recentment és la de vehicles autònoms. Els investigadores estan en este moment centrats en el tercer dels cinc nivells d'autonomia, els quals són: assistència en la conducció, automatització parcial, automatització condicional, alta automatització i automatització completa. Malgrat els ràpids progressos que s'estan donant en este camp, l'adopció d'estes solucions portarà temps no sols degut a qüestions legals, sinó també pel fet que els avanços tecnològics s'enfronten a un lent recolzament per part dels fabricants. A més a més, la baixa taxa de renovació de vehicles de carretera, dificulta el desplegament de tecnologies innovadores com és el cas de la xarxa vehicular. Huit anys després de la introducció de la norma 802.11p per a la comunicació vehicular de l'Institut d'Enginyers Elèctrics i Electrònics (IEEE), els vehicles que s'utilitzen a diari encara manquen de la capacitat de comunicar-se entre sí. Este fet impedeix l'ús de les moltes aplicacions de seguretat del Sistema d'Intel·ligència de Transport (SIT) que aprofita la xarxa vehicular per a l'intercanvi de dades. La forma òbvia de tractar aquest problema és posar les tecnologies disponibles a la disposició dels usuaris comuns per a desenvolupar solucions que es puguen implementar fàcilment, còmodes d'adoptar i, a més a més, econòmiques.
Per aquesta raó, traslladem la nostra atenció als dispositius intel·ligents, especialment als telèfons intel·ligents, els quals han recorregut un llarg camí des de la primera introducció de telèfons mòbils a finals del segle XX. Hui en dia quasi tots porten un en la butxaca on siga que vagen, permetent-los no sols fer cridades, sinó també mesurar i controlar diferents paràmetres amb l'ajuda dels molts sensors integrats que estan disponibles per a estos dispositius compactes però potents. El nostre objectiu és estudiar els efectes de la integració dels telèfons intel·ligents a la xarxa vehicular per a desenvolupar aplicacions de seguretat del SIT. L'elecció dels telèfons intel·ligents ací no està sols justificada per la seua àmplia disponibilitat i ús, sinó també perquè estan evolucionant cap a terminals d'alt rendiment amb microprocessadors de múltiples nuclis dotats amb un grup suficientment divers de sensors. En esta tesi proposem tres diferents aplicacions de seguretat SIT per a telèfons intel·ligents, dissenyats per a aprofitar l'entorn de xarxa vehicular: una aplicació de generació d'advertència anomenada Messiah que alerta els conductors de la presència de vehicles d'emergència en les proximitats; una aplicació Advertència de Col·lisió Frontal (ACF) que adverteix els conductors si no mantenen la distància de seguretat mínima entre el vehicle que va davant i el que el segueix; i, per últim, una aplicació que té com objectiu ajudar els conductors amb assistència visual durant l'avançament, anomenat EYES. Totes aquestes aplicacions han sigut desenvolupades per a la plataforma Android, i depenen de la transmissió de dades entre vehicles. Donat que els vehicles que utilitzem a diari no admeten la possibilitat de comunicar-se entre sí, també dissenyem GRCBox, que és una unitat integrada de baix cost que permet la comunicació de Vechicle a Tot (V2X).
A partir del nostre estudi d'aplicacions per a dispositius mòbils dissenyats per a xarxes vehiculars, descobrim que l'ús de telèfons intel·ligents proporciona una nova direcció per a la investigació relacionada amb SIT i xarxes vehiculars al permetre l'adopció ràpida de les solucions existents, on els usuaris poden descarregar i utilitzar les aplicacions amb un sol clic a un botó. Però al mateix temps, la portabilitat i la compacitat dels dispositius els fa limitats en termes de velocitat, potència de processament i precisió del sensor integrat, cosa que afecta al rendiment de les aplicacions.[EN] One of the research areas that is receiving a lot of attention recently is autonomous vehicles. Researchers are currently focused on the third level of autonomy out of the five levels, which are: drive assistance, partial automation, conditional automation, high automation, and full automation. Even though rapid progress is being made in this field, the adoption of these solutions will take time not only due to legal issues, but also due to the fact that technological improvements face slow endorsement by manufacturers. Also, the slow renewal rate of vehicles on road hinders the deployment of novel technologies, as is the case of Vehicular Networks (VNs). Eight years after the introduction of the IEEE 802.11p standard for vehicular communication, vehicles used on a daily basis still lack the capability of communicating with one other. This fact impedes the use of the many ITS safety applications that take advantage of VNs for data exchange. The obvious way to handle this problem is to use the available technologies at the disposal of common users to develop solutions that are easily deployable, effortless to adopt, and moreover, cost effective.
For this reason we shift our attention to smart devices, specially smartphones, which have come a long way since the first introduction of mobile phones in the late 20th century. Nowadays, nearly everyone carries one in their pocket anywhere they go, allowing them to not only make calls, but also to measure and monitor different parameters with the help of the many on-board sensors that are available to these compact yet powerful devices. Our objective is to study the effects of integrating smartphones to vehicular networks, to develop ITS safety applications. The choice of smartphones here is not only justified by their wide availability and use, but also because they are evolving towards high performance terminals with multi-core microprocessors packed with a sufficiently diverse group of sensors. In this thesis we propose three different ITS safety applications for smartphones, designed to take advantage of the vehicular network environment: a warning generation application called Messiah that alerts drivers of the presence of emergency vehicles in close proximity; a FCW application which warns drivers if a minimum safe distance is not maintained between the vehicle ahead and the one following it; and lastly an application that aims to aid drivers with visual assistance while overtaking, named EYES. All these applications have been developed for the Android platform, and are dependent on the data transmission among vehicles. Since vehicles we use on a day to day basis still do not accommodate the possibility to communicate with one another, we also designed the GRCBox, which is a low cost on-board unit that supports V2X communication.
From our study of applications for mobile devices designed for VNs, we found that the use of smartphones provides a new direction to research related to ITS and VNs by allowing a quick adoption of the existing solutions, where users are able to download and use applications just by one click of a button. But at the same time, the portability and compactness of the devices makes them limited in terms of speed, processing power, and accuracy of the on-board sensor, thus affecting the performance of the applications. In our case, the simpler Messiah application performed very well, while the EYES application that is dependent on GPS data, and the FCW application which required heavy processing and use of the camera due to its dependence on plate recognition, were affected by the hardware limitations of the smartphones.Patra, S. (2019). Development and evaluation of smartphone-based ITS applications for vehicular networks [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/124058TESI
A study of parasympathetic autonomic function in different phases of the menstrual cycle among young adult females
No full textBackground: The sex hormones, estrogen, and progesterone, as well as the gonadotropins, luteinizing hormone, and follicle-stimulating hormone produced from the anterior pituitary, govern the three stages of the menstrual cycle: menstrual, follicular, and luteal. Hormone fluctuations cause changes in autonomic function. There have been relatively few studies that show autonomic function modification throughout various periods of the menstrual cycle. Aim and Objectives: The current study was undertaken to investigate the difference in mean parasympathetic autonomic characteristics between the proliferative and secretory stages of the menstrual cycle. Materials and Methods: A total of 120 healthy young adult female volunteers were recruited among Kalinga Institute of Medical Sciences, Kalinga Institute of Nursing Sciences, and Kalinga Institute of Dental Sciences students. The study eliminated 20 students who were in the bleeding phase of their cycle. The electrocardiogram was captured, and parasympathetic parameters were determined using the fully automated CANWin instrument in the Department of Physiology. The data of the abovementioned parameters were compiled, tabulated, and entered into Microsoft Excel 2013 and statistically analyzed using STATA software 15.1. Results: The subjects' mean ages, heights, and weights were 18.9 ± 0.70 years, 156.6 ± 5.25 cm, and 53.7 ± 8.63 kg, respectively. The mean resting heart rate in the secretory phase was substantially greater than in the proliferative phase, indicating sympathetic dominance. The 30:15 ratio between proliferative and secretory stages. The mean E: I ratio calculated from the slow deep breathing maneuver reveals a statistically significant difference between the proliferative and secretory phases. Conclusion: During the secretory phase, there is a considerable rise in parasympathetic activity among the individuals in the current study. Changes in parasympathetic activity throughout the follicular and secretory stages of the menstrual cycle may be caused by fluctuations in estrogen and progesterone levels
Modeling of mechanical energy dissipation of low-dimensional resonators
No full textNanoelectromechanical systems (NEMS) made from low-dimensional materials based on carbon, transition metal dichalcogenides, and their combinations have opened up new possibilities in high-precision sensing, signal processing, and studies of fundamental physical phenomena. In the heart of the NEMS is a vibrating mechanical element, known as the resonator. The performance of the NEMS critically depends on the mechanical energy dissipated by the resonator. Studies on dissipation are important because an understanding of the loss mechanisms can suggest ways to mitigate it. In most practical scenarios, the resonators suffer from intrinsic dissipation mediated by its inherent atomic thermal motions or phonons and extrinsic dissipation due to a fluid environment. In this context, low-dimensional resonators need special attention because the dissipation cannot be explained using the existing continuum theories. Due to atomic thickness, sub-micron dimension, and mega- to gigahertz frequencies of these resonators, nano-scale physical processes start becoming important. Most macroscale models do not account for these physical processes, warranting the current line of research. In this thesis, we use atomistic simulations and statistical-mechanical theories to understand and formulate the nanoscale physical processes, and integrate them to develop a multiscale model for dissipation.
In the first part of the thesis, we explore fluid coupled resonator systems with an objective to understand different dissipative processes such as phonon-mediated intrinsic dissipation, viscous damping by the fluid, and the cross-interaction between each source of dissipation, i.e., phonons and fluid at a regime of gigahertz frequency, and nanometer length scale. First, we consider a single-walled carbon nanotube (SWCNT) resonator with confined Argon and driven under axial mode. The intrinsic dissipation in the SWCNT at gigahertz frequencies could be explained by Akhiezer theory. We show that intrinsic dissipation, which is conventionally treated as an independent process, can be modified by fluid interactions due to the phonon- fluid coupling. We show that an important consequence of this phonon-fluid coupling is the counter-intuitive inverse scaling of net dissipation with fluid density at low excitation frequencies. Next, we consider flexural vibration of the SWCNT with interior and exterior Argon. When compared with the fluid exterior case, the SWCNT with confined fluid shows a low and anomalous scaling of dissipation with fluid density. We systematically analyzed the sources of dissipation and found that the fluid contributed to the anomalous scaling. A formulation of the fluid response during the flexural motion revealed a viscoelastic nature of the fluid under nano-confinement, which explains the anomalous scaling. Further, we use the framework for dissipation analysis to examine the effect of thermal motion of the resonator atoms on fluid dissipation, demonstrate a frequency dependent dissipation scaling with density, and comment on the mechanism of intrinsic dissipation during flexural resonance of an SWCNT.
In the second part, we develop a multiscale framework to model intrinsic dissipation in two-dimensional (2D) microresonators. The work aims to reveal the fundamental limit of dissipation and enable looking at the isolated effect of various parameters over a wide range, both of which are inaccessible in experiments. The damping of the flexural mode of a 2D microresonator takes place due to the nonlinear coupling with other thermally excited elastic modes. A particular flexural mode can couple with another flexural mode with a wavelength ranging from the size of the resonator to that of the lattice spacing. However, the coupling at these disparate length scales needs different modeling approaches. In the multiscale framework, we model the continuum-scale modes as Langevin oscillators (LOs) with nonlinear coupling terms. The parameters of the LOs are computed using continuum mechanical analysis and atomistic simulations. Using this framework, we study the effect of various parameters of interest such as vibration amplitude, resonator size, temperature, and pre-strain in the case of graphene resonators and draw some important conclusions towards engineering high-quality 2D resonators.Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2021-05-01The student, Subhadeep De, accepted the attached license on 2019-02-13 at 12:01.The student, Subhadeep De, submitted this Dissertation for approval on 2019-02-13 at 12:51.This Dissertation was approved for publication on 2019-02-15 at 11:34.DSpace SAF Submission Ingestion Package generated from Vireo submission #13388 on 2019-08-22 at 15:04:21Made available in DSpace on 2019-08-23T20:28:06Z (GMT). No. of bitstreams: 3
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An Isothiazolanthrone‐Based Self‐Assembling Anticancer Color‐Changing Dye for Concurrent Imaging and Monitoring of Cell Viability
No full textWe report the photophysical properties, self-assembly and biological evaluation of an isothiazolanthrone-based dye, 7-amino-6H-anthra[9,1-cd]isothiazol-6-one (AAT), which reveals anticancer properties and can be potentially used as dye for monitoring cell viability. The solvent-dependent photophysical studies suggest that the emission of AAT is sensitive to environment polarity due to which interesting changes in the colored emission may be observed owing to the charge transfer (CT) processes. AAT also self-assembles to tree-like branched morphologies and produce, a greenish emission inside the cells when imaged after short interval (15 mins) of incubation while a red fluorescence could be noted after 24 h. Interestingly, AAT also produce differential emission inside mouse normal cells as compared to its cancer cell lines since it possess anticancer activity. The experimental observations were also validated theoretically via computational modeling. © 2023 Wiley-VCH GmbH.Indian Council of Medical Research, ICMR: 45/13/2020‐/BIO/BMS; Science and Engineering Research Board, SERB: EMR/2016/003186, SPG/2021/000521; Ministry of Education and Science of the Russian Federation, Minobrnauka: FEUZ‐2023‐0013NG, greatly acknowledge support from the SERB research grants (EMR/2016/003186; SPG/2021/000521) for funding. VK is grateful to ICMR, India for providing financial support under the Senior Research Fellowship No. 45/13/2020‐/BIO/BMS. BK thanks SHODH fellowship for funding and Indrashil University for infrastructure support. RS thanks CSIR‐UGC for the pre‐doctoral senior research fellowship. KBJ acknowledges Sophisticated Instrument Centre (SIC)‐Dr. Harisingh Gour Central University Sagar, India for AFM facility. DWB acknowledged support Ministry of Science and Higher Education of the Russian Federation (through the basic part of the government mandate, Project No. FEUZ‐2023‐0013) and Jiangsu Innovative and Entrepreneurial Talents Project. NG thanks Dr. Abhijit Patra, Mr. Subhadeep Das and Mr. Subhankar Kundu, IISER Bhopal, for TCSPC sample analysis and quntum yield measurement using solid state fluorescence..NG, greatly acknowledge support from the SERB research grants (EMR/2016/003186; SPG/2021/000521) for funding. VK is grateful to ICMR, India for providing financial support under the Senior Research Fellowship No. 45/13/2020-/BIO/BMS. BK thanks SHODH fellowship for funding and Indrashil University for infrastructure support. RS thanks CSIR-UGC for the pre-doctoral senior research fellowship. KBJ acknowledges Sophisticated Instrument Centre (SIC)-Dr. Harisingh Gour Central University Sagar, India for AFM facility. DWB acknowledged support Ministry of Science and Higher Education of the Russian Federation (through the basic part of the government mandate, Project No. FEUZ-2023-0013) and Jiangsu Innovative and Entrepreneurial Talents Project. NG thanks Dr. Abhijit Patra, Mr. Subhadeep Das and Mr. Subhankar Kundu, IISER Bhopal, for TCSPC sample analysis and quntum yield measurement using solid state fluorescence
An intelligent transportation system application for smartphones based on vehicle position advertising and Route Sharing in Vehicular Ad-Hoc Networks
No full text[EN] Alerting drivers about incoming emergency vehicles and their routes can greatly improve their travel times in congested cities, while reducing the risk of accidents due to distractions. This paper contributes to this goal by proposing Messiah, an Android application capable of informing regular vehicles about incoming emergency vehicles like ambulances, police cars and fire brigades. This is made possible by creating a network of vehicles capable of directly communicating between them. The user can, therefore, take driving decisions in a timely manner by considering incoming alerts. Using the support of our GRCBox hardware, the application can rely on vehicular ad-hoc network communications in the 5 GHz band, being V2V (vehicle-to-vehicle) communication provided through a combination of Android-based smartphone and our GRCBox device. The application was tested in three different scenarios with different levels of obstruction, showing that it is capable of providing alerts up to 300 meters, and notifying vehicles within less than one secondThis work was partially supported by the "Ministerio de Economia y Competividad, Programa Estatal de Investigacion, Desarollo e Innovacion Orientada a los Retos de la Sociedad, Proyectos I+D+I 2014", Spain, under Grant Nos. TEC2014-52690-R and BES-2015-075988.Hadiwardoyo, SA.; Patra, S.; Tavares De Araujo Cesariny Calafate, CM.; Cano, J.; Manzoni, P. (2018). An Intelligent Transportation System Application for Smartphones Based on Vehicle Position Advertising and Route Sharing in Vehicular Ad-Hoc Networks. Journal of Computer Science and Technology. 33(2):249-262. https://doi.org/10.1007/s11390-018-1817-4S249262332Papadimitratos P, De La Fortelle A, Evenssen K, Brignolo R, Cosenza S. 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Demo: Video-Based Overtaking Assistance Now A Reality
No full text© 2015 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.ITS solutions suffer from the slow pace of adoption
by manufacturers despite the interest shown by both consumers
and industry. Our goal is to develop ITS applications using
already available technologies to make them affordable, quick
to deploy, and easy to adopt. In this paper we introduce an ITS
system for overtaking assistance that provides drivers with a
real-time video feed from the vehicle located just in front. This
provides a better view of the road ahead, and of any vehicles
travelling in the opposite direction, being especially useful when
the front view of the driver is blocked by large vehicles.This work was partially supported by the European Commission under Svägata.eu, the Erasmus Mundus Programme, Action 2 (EMA2) and the Ministerio de Economía y Competitividad, Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad, Proyectos I+D+I 2014, Spain, under Grants TEC2014-52690-R and BES-2012-052673.Patra, S.; Tornell, SM.; Tavares De Araujo Cesariny Calafate, CM.; Cano Escribá, JC.; Manzoni, P. (2015). Demo: Video-Based Overtaking Assistance Now A Reality. IEEE. https://riunet.upv.es/handle/10251/64635
Three in One: Stimuli-Responsive Fluorescence, Solid-State Emission, and Dual-Organelle Imaging Using a Pyrene-Benzophenone Derivative
No full textSmall
organic luminogens, owing to their contrasting stimuli-responsive
fluorescence in solution along with strong emission in aggregated
and solidstates, have been employed in optoelectronic devices, sensors,
and bioimaging. Pyrene derivatives usually exhibit strong fluorescence
and concentration-dependent excimer/aggregate emission in solution.
However, the impacts of microenvironments on the monomer and aggregate
emission bands and their relative intensities in solution, solid,
and supramolecular aggregates are intriguing. The present study delineates
a trade-off between the monomer and aggregate emissions of a pyrene-benzophenone
derivative (ABzPy) in solution, in the solid-state, and in nanoaggregates
through a combined spectroscopic and microscopic approach. The impact
of external stimuli (viscosity, pH) on the aggregate emission was
demonstrated using steady-state and time-resolved spectroscopy, including
fluorescence correlation spectroscopy and fluorescence anisotropy
decay analysis. The aggregate formation was noticed at a higher concentration
(>10 μM) in solution, at 77 K (5 μM), and in the solid-state
due to the π–π stacking interactions (3.6 Å)
between two ABzPy molecules. In contrast, no aggregate formation was
observed in the viscous medium as well as in a micellar environment
even at a higher concentration of ABzPy (50 μM). The crystal
structure analysis further shed light on the intermolecular hydrogen-bonding-assisted
solid-state emission, which was found to be highly sensitive toward
external stimuli like pH and mechanical forces. The broad emission
band comprising both monomer and aggregate in the aqueous dispersion
of nanoaggregates was used for the specific cellular imaging of lysosomes
and lipid droplets, respectively
EXPERIMENTAL INVESTIGATIONS ON THE SURFACE-DRIVEN CAPILLARY FLOW OF AQUEOUS MICROPARTICLE SUSPENSIONS IN THE MICROFLUIDIC LABORATORY-ON-A-CHIP SYSTEMS
No full textIn this work, total 1592 individual leakage-free polymethylmethacrylate (PMMA) microfluidic devices as laboratory-on-a-chip systems are fabricated by maskless lithography, hot embossing lithography, and direct bonding technique. Total 1094 individual Audio Video Interleave Files as experimental outputs related to the surface-driven capillary flow have been recorded and analyzed. The influence of effective viscosity, effect of surface wettability, effect of channel aspect ratio, and effect of centrifugal force on the surface-driven microfluidic flow of aqueous microparticle suspensions have been successfully and individually investigated in these laboratory-on-a-chip systems. Also, 5 micron polystyrene particles have been separated from the aqueous microparticle suspensions in the microfluidic lab-on-a-chip systems of modified design with 98% separation efficiency, and 10 micron polystyrene particles have been separated with 100% separation efficiency. About the novelty of this work, the experimental investigations have been performed on the surface-driven microfluidic flow of aqueous microparticle suspensions with the investigations on the separation time in particle-size based separation mechanism to control these suspensions in the microfluidic lab-on-a-chip systems. This research work contains a total of 10,112 individual experimental outputs obtained using total 30 individual instruments by author’s own hands-on completely during more than three years continuously. Author has performed the experimental investigations on both the fluid statics and fluid dynamics to develop an automated fluid machine.</jats:p
Performance tuning of a smartphone-based overtaking assistant
No full textITS solutions suffer from the slow pace of adoption by manufacturers despite the interest shown by both consumers and industry. Our goal is to develop ITS applications using already available technologies to make them affordable, quick to deploy, and easy to adopt. In this paper we introduce EYES, an overtaking assistance solution that provides drivers with a real-time video feed from the vehicle located just in front. Our application thus provides a better view of the road ahead, and of any vehicles travelling in the opposite direction, being especially useful when the front view of the driver is blocked by large vehicles. We evaluated our application using the MJPEG video encoding format, and have determined the most effective resolution and JPEG quality choice for our case. Experimental results from the tests performed with the application in both indoor and outdoor scenarios, allow us to be optimistic about the effectiveness and applicability of smartphones in providing overtaking assistance based on video streaming in vehicular networks
Unveiling Autophagy and Aging Through Time-Resolved Imaging of Lysosomal Polarity with a Delayed Fluorescent Emitter
No full textDetecting the lysosomal microenvironmental changes like viscosity, pH, and polarity during their dynamic interorganelle interactions remains an intriguing area that facilitates the elucidation of cellular homeostasis. The subtle variation of physiological conditions can be assessed by deciphering the lysosomal microenvironments during lysosome-organelle interactions, closely related to autophagic pathways leading to various cellular disorders. Herein, we shed light on the dynamic lysosomal polarity in live cells and a multicellular model organism, Caenorhabditis elegans (C. elegans), through time-resolved imaging employing a thermally activated delayed fluorescent probe, DC-Lyso. The highly photostable and cytocompatible DC-Lyso rapidly labels the lysosomes (within 1 min of incubation) and exhibits red luminescence and polarity-sensitive long lifetime under the cellular environment. The distinct variation in the fluorescence lifetime of DC-Lyso suggests an increase in local polarity during the lysosomal dynamics and interorganelle interactions, including lipophagy and mitophagy. The lifetime imaging analysis reveals increasing lysosomal polarity as an indicator for probing the successive development of C. elegans during aging. The in vivo microsecond timescale imaging of various cancerous cell lines and C. elegans, as presented here, therefore, expands the scope of delayed fluorescent emitters for unveiling complex biological processes
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