2,573,588 research outputs found
PAS a PAS fins Nadal
No full textDe l'1 al 24 de desembre descobreix el ‘PAS a PAS fins Nadal’, un calendari d’Advent en format audiovisual per visibilitzar com el personal tècnic, de gestió i d’administració i serveis (PTGAS) contribueix a fer comunitat amb la seva feina diària i a assolir els objectius i reptes de la Universitat
PAS a PAS fins Nadal
No full textDe l'1 al 24 de desembre descobreix el ‘PAS a PAS fins Nadal’, un calendari d’Advent en format audiovisual per visibilitzar com el personal tècnic, de gestió i d’administració i serveis (PTGAS) contribueix a fer comunitat amb la seva feina diària i a assolir els objectius i reptes de la Universitat
[Délibérations et procès-verbaux du conseil général du département du Pas-de-Calais] ([Reprod.])
No full textCollection : Les archives de la Révolution française ; 6.1.103Collection : Les archives de la Révolution française ; 6.1.103Appartient à l’ensemble documentaire : NordPdeC
Factor structure of the PAS-ADD Checklist with adults with intellectual disabilities
Get PDFBackground The PAS-ADD Checklist is designed to screen for likely mental health problems in people with intellectual disabilities (ID). The specificity of recommended subscales derived from diagnostic criteria is unclear. This paper therefore investigates the factor structure of the PAS-ADD Checklist to determine the adequacy of empirically derived subscales. Method A total of 1,115 informants who had known service users for a median of 24 months completed the PAS-ADD Checklist on 1,155 adults with ID living either in the community, in residential care, or in hospital settings in a county in North-East England. Results The sample was randomly divided into two, with all item scores dichotomised. An exploratory principal components factor analysis with varimax rotation was conducted on Subsample A, producing an optimal 7-factor solution. However, a confirmatory factor analysis using this factor structure for Subsample B revealed a mediocre to poor fit. Further exploratory and confirmatory factor analyses also indicated that empirically derived PAS-ADD Checklist subscales were inconsistent. Conclusion Given the inconsistency of empirically derived subscales, we do not recommend using the PAS-ADD Checklist to identify specific types of psychopathology. The Checklist may have more utility as a screening tool for general psychopathology and subsequent referral for more detailed assessment
District de Boulogne : état général des biens appartenans à des personnes dont le domicile n'est point connu dans le département du Pas-de-Calais ([Reprod.])
No full textCollection : Les archives de la Révolution française ; 6.1.114Collection : Les archives de la Révolution française ; 6.1.114Appartient à l’ensemble documentaire : NordPdeC
Structural and functional analyses of PAS domain interactions of the clock proteins Drosophila PERIOD and mouse PERIOD2
Get PDFPERIOD proteins are central components of the Drosophila and mammalian circadian clocks. The crystal structure of a Drosophila PERIOD (dPER) fragment comprising two PER-ARNT-SIM (PAS) domains (PAS-A and PAS-B) and two additional C-terminal alpha-helices (alphaE and alphaF) has revealed a homodimer mediated by intermolecular interactions of PAS-A with tryptophane 482 in PAS-B and helix alphaF. Here we present the crystal structure of a monomeric PAS domain fragment of dPER lacking the alphaF helix. Moreover, we have solved the crystal structure of a PAS domain fragment of the mouse PERIOD homologue mPER2. The mPER2 structure shows a different dimer interface than dPER, which is stabilized by interactions of the PAS-B beta-sheet surface including tryptophane 419 (equivalent to Trp482dPER). We have validated and quantitatively analysed the homodimer interactions of dPER and mPER2 by site-directed mutagenesis using analytical gel filtration, analytical ultracentrifugation, and co-immunoprecipitation experiments. Furthermore we show, by yeast-two-hybrid experiments, that the PAS-B beta-sheet surface of dPER mediates interactions with TIMELESS (dTIM). Our study reveals quantitative and qualitative differences between the homodimeric PAS domain interactions of dPER and its mammalian homologue mPER2. In addition, we identify the PAS-B beta-sheet surface as a versatile interaction site mediating mPER2 homodimerization in the mammalian system and dPER-dTIM heterodimer formation in the Drosophila system
p160/SRC/NCoA coactivators form complexes via specific interaction of their PAS-B domain with the CID/AD1 domain
No full textTranscriptional activation involves the ordered recruitment of coactivators via direct interactions between distinct binding domains and recognition motifs. The p160/SRC/NCoA coactivator family comprises three members (NCoA-1, -2 and -3), which are organized in multiprotein coactivator complexes. We had identified the PAS-B domain of NCoA-1 as an LXXLL motif binding domain. Here we show that NCoA family members are able to interact with other full-length NCoA proteins via their PAS-B domain and they specifically interact with the CBP-interaction domain (CID/AD1) of NCoA-1. Peptide competition, binding experiments and mutagenesis of LXXLL motifs point at distinct binding motif specificities of the NCoA PAS-B domains. NMR studies of different NCoA-1-PAS-B/LXXLL peptide complexes revealed similar although not identical binding sites for the CID/AD1 and STAT6 transactivation domain LXXLL motifs. In mechanistic studies, we found that overexpression of the PAS-B domain is able to disturb the binding of NCoA-1 to CBP in cells and that a CID/AD1 peptide competes with STAT6 for NCoA-1 in vitro. Moreover, the expression of an endogenous androgen receptor target gene is affected by the overexpression of the NCoA-1 or NCoA-3 PAS-B domains. Our study discloses a new, complementary mechanism for the current model of coactivator recruitment to target gene promoters
[Dossier sur le 20 juin 1792] ([Reprod.])
No full textCollection : Les archives de la Révolution française ; 6.1.104Collection : Les archives de la Révolution française ; 6.1.104Comprend : Arrêté du directoire du département du Nord ; Adresse du directoire du département du Nord à l'Assemblée nationale ; Adresse du directoire du département du Nord, au roi des François ; Extrait du registre aux délibérations du directoire du district de Béthune, séance du 28 juin 1792 ; Extrait du registre aux délibérations du directoire du district de Montreuil-sur-Mer , séance du trois juillet mil sept cent quatre-vingt-douze... ; Extrait des registres aux arrêtés du directoire du district de Montreuil ; Extrait du registre aux délibérations du district de Saint-OmerAppartient à l’ensemble documentaire : NordPdeC
PAS-induced effects.
No full textMean MEP amplitudes following PAS are shown relative to the baseline level. Each study is represented by a distinct color as in Figs 2 and 3, each circle represents the measurements of one subject, and each black diamond marker represents the mean of a study at the given time-point. The black line with error bars represents the temporal course of PAS across subjects and studies. The dashed red line represents no change against the baseline measurement. Left panel: normalized data ± SEM. Right panel: same PAS data but log-transformed prior to calculation of means and SEM. Note that the x-axis depicts distinct time points rather than a continuous scale.</p
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