1,720,972 research outputs found
Plantaricin A of Lactobacillus plantarum IYP1718 playing a role in controlling Undesirable Organisms in Soil
Get PDFLactobacillus plantarum can control undesirable
organisms in soil to create a balanced environment that
can protect plant life from diseases by producing
plantaricin A. L. plantarum from two conditions was
isolated. Six isolates from healthy soil and four isolates
from soil that have black rot disease in brassica plants.
Existence of plnA in each L. plantarum isolate was
demonstrated by PCR using gene-specific primers.
Three out of six L. plantarum isolates from healthy soil
and one out of four L. plantarum isolates from soil have
black rot disease in brassica plant showing the
presence of plnA. L. plantarum IYP1718 from soil that
has black rot disease in brassica plants, was chosen for
sequence analysis.
The amplified PCR fragment was confirmed to have
100% similarity with the plnA sequence in GenBank. A
phylogenetic tree based on sequence analysis
confirmed that plnA has closeness with plantaricin G
and similarity with enterococcin from Enterococcus
faecalis BFE 1071 based on the pairwise distance.
Sequence analysis showed that there are 4 ORFs, but
only 2 ORFs could be identified. The results of this
study imply that L. plantarum plays a role in
controlling undesirable organisms in soil by producing
plnA
Diversity of arbuscular mycorrhiza and maize yield in Cajeput agroforestry system with different fertilizer management
Get PDFThis research was conducted with the aims: the fi rst, to study diversity of arbuscular mycorrhiza in maize rhizosphere
with different fertilizer management in Cajeput (Melaleuca leucadendron LINN.) agroforestry system in Ponorogo, East Java, Indonesia; the second, to detect the correlation between diversity of arbuscular mycorrhiza with soil fertility (nutrient content of the soil) and maize yield. Fertilizer management was comprised to conventional management (CM), universal management (UM), and alternative managements (AM1, AM2, and AM3). Plot of CM used low inorganic fertilizer and chicken manure. Plot of UM and AM3 used medium inorganic fertilizer and chicken manure. Plot of AM1 used medium inorganic fertilizer and high chicken manure. Plot of AM2 used high inorganic fertilizer. Specially plot of AM2 used maize straw and AM3 used maize straw plus intensive tillage. Results of the research showed that diversity index value of arbuscular mycorrhiza in the plot of AM2 was highest and in the plot of AM1 was lowest. There were four genera of arbuscular mycorrhiza identifi ed: Glomus,
caulospora, Gigaspora and Scutellospora. The dominant species in all fertilizer management was Glomus contrictum.
Glomus minutum was found only in the plot of CM, Scutellospora colospora in the plot of UM, and Acaulospora favoeta in the plot of AM3. Infection of arbuscular mycorrhiza in roots maize ranged from 34.62% to 44.77% and spore density ranged from115 to 195 spore/100g of soil in fi ve fertilizer management. Infection of arbucular mycorrhiza and spore density were higest in plot of AM2, but maize yield was higest in the plot of AM1. Diversity index value of arbuscular mycorrhiza (species richness) has negative correlation with nutrient content of the soil and maize yield
Growth and yield of Shallot (Allium cepa L.) in respons of organic fertilizers and Trichoderma asperellum
Get PDF. Increased shallot yield with aplication of organic fertilizer. Cajeput waste as a source
of organic fertilizer is very abundant in Ponorogo. Research aimed to study the aplication of
organic fertilizers and Trichoderma asperellum to incerase growth and yield of shallot. The
experiment was laid-out in factorial randomized completely design with 3 replications and eight
treatment combinations. The first factors is organic fertilizer ( without organic fertilizer; 6 ton
ha-1 of cajeput waste compost, 3 ton ha-1 of cajeput waste compost + 3 ton ha-1 of rabbit manure,
6 ton ha-1 of rabbit manure). The second factors is Trichoderma asperellum (without T.
asperellum and 1 L ha-1 of T. asperellum). The results of this study showed that treatmen of 3
ton ha-1 of cajeput waste compost + 3 ton ha-1 of rabbit manure without Trichoderma asperellum
increased plant hight and leaf dry weight of shallot by 25% and 135,66%. The application of
combination rabbit manure and without T. asperellum increased shallot tillering number by 9,67
tillering. The application of 3 ton ha-1 of cajeput waste compost + 3 ton ha-1 of rabbit manure
increased shallot yield and diameter of shallot bulbs
RATE AND QUALITY OF DECOMPOSITION CAJEPUT WASTE WAS EFFECTED BY BIOACTIVATORS
Get PDFCajeput waste has the potential to source compost but has high C/N and essential oils so that
decomposition naturally takes a long time. The objective of the study was to find an appropriate method in
the decomposition of Cajeput waste through the addition of bioactivators. The bioactivators used are
Aspergilus, Trichoderma, a combination of Aspergilus + Trichoderma and MA-11. Aspergillus and Trichoderma
were obtained by isolation from Cajeput waste, while MA-11 was a bioactivator from outside the Cajeput
waste. The study was conducted using a complete randomized design with five treatments and three
replications. Observation parameters were C, N, C/N, P and K levels. The results showed that the addition
of bioactivator can accelerate the process of decomposition of Cajeput waste. The decomposition of Cajeput
waste with the addition of bioactivator takes 10 weeks, whereas without bioactivator takes more than 12
weeks. The best bioactivator is Trichoderma, capable of producing compost fertilizer with N content of 1.56
± 0.10%, K of 1.02 ± 0.10% and C / N of 16.58 ± 2.37
PENGARUH DOSIS PUPUK NITROGEN DAN KALIUM TERHADAP PERTUMBUHAN DAN HASIL TANAMAN KEDELAI ( Glycine Max L) VARIETAS WILLS.
Get PDFTujuan penelitian ini adalah untuk mengetahui sejauh mana pengaruh
dosis pupuk Nitrogen dan Kalium terhadap pertumbuhan dan hasil tanam kedelai.
Percobaan ini dilakukan dilahan sawah yang letaknya di desa Tonatan, Kecematan
Ponorogo, Kabupaten Ponorogo pada ketinggian tempat 115 m dpl dengan jenis
tanah grumosol. Percobaan dilakukan pada bulan Mei sampai Juli 1991, dengan
menggunakan rancangan percobaan secara faktorial dalam pola dasar rancangan
acak kelompok yang terdiri dari 2 faktor dan diulan 3 kali. Faktor pertama dosis
pupuk nitrogn terdiri 4 level yaitu N0 = tanpa dipupuk N, N1 = dipupuk dengan
dosis 50 kg/ha, N2 = dipupuk dengan dosis 100 kg/ha, N3 = dipupuk dengan dosis
150 kg/ha. Faktor kedua ppuk Kalium terdir dari 3 level, yaitu : K0 = tanpa
dipupuk, k1 = dipupuk dengan dosis 50 kg/ha, K2 = dipupuk dengan dosis 100
kg/ha. Variebel yang diamati adalah tinggi tanaman, jumlah daun, jumlah cabang,
jumlah polong, berat brangkasan basah, berat brangkasan kering, hasil biji per 1
m2
dengan berat biji per 100 butir. Hasil percobaan menunjukkan bahwa dosis
pemupukan Nitrogen dan Kalium berpengaruh sangat nyata terhadap tinggi berat
brangkasan basah, berat brangkasan kering, hasil biji per 1 m2
dengan berat biji
per 100 butir. Pada perlakuan kombinasi pemupukan 100 kg N/ha dan 50 kg K/ha
(N2K1) memberikan hasil tertinggi pada semua variabel pengamatan, sedangkan
hasil terendah ditunjukkan oleh perlakuan kombinasi tanpa pemupukan Nitrogen
dan tanpa pemupukan Kalium (N0K0)
Isolasi dan Identifikasi Fungi Mikoriza pada Rizosfer Tanaman Porang pada Sistem Agroforestri dan Monokultur
Get PDFMikoriza arbuskula adalah mikrobia tanah yang memiliki peran dalam meningkatkan ketersediaan air
bagi tanaman melalui perpanjangan hifa sehingga dapat menjangkau pada daerah yang lebih luas.
Mikoriza arbuskula merupakan mikrobia tanah yang dapat berkembang pada sistem agroforestri dan
monokultur. Informasi mikoriza yang dapat bersimbiosis dengan Porang baik secara agroforestri dan
monokultur belum banyak dipublikasikan. Oleh sebab itu perlu identifikasi spora yang ada di rizosfer
Porang sehingga nantinya dapat dijadikan sebagai bahan baku pupuk berbasis mikoriza arbuskula untuk
tanaman Porang. Penelitian ini bertujuan untuk mengidentifikasi mikoriza arbuskula yang berada di
rizosfer Porang pada berbagai sistem pertanaman. Penelitian tentang fungi mikoriza arbuskula di rizosfer
Porang dilakukan di lahan agroforestri dan monokultur yang memiliki jenis tanah latosol. Lahan
penelitian berada di Desa Mrayan, Kecamatan Ngrayun kabupaten Ponorogo Jawa timur Indonesia dan
dilakukan pada bulan Desember 2020. Sampel tanah diambil secara acak di tiga lahan yang berbeda yaitu
agroforestri berbasis pinus, agroforestri berbasis sengon dan monokultur Porang. Sampel tanah diambil
disekitar perakaran tanaman Porang. Sampel akar berupa akar halus porang. Spora mikoriza arbuskula
diektrak dengan metode pengayaan basah dan kering. Identifikasi spora mikoriza arbuskula dilakukan
dengan identifikasi morfologi. Hasil penelitian ini ditemukan 3 genera yang terdiri dari 9 species yaitu
Glomus (5 species), Acaulospora (2 speies) dan Gigaspora (2 species). Kepadatan spora tertinggi berjenis
Glomus dan yang terkecil adalah Gigaspora. Rata rata kepadatan spora adalah 56-105 spora/100 g tanah.
Kepadatan spora tertinggi pada agroforestri berbasis pinus. Persentase infeksi akar berkisar antar 24-
50%. Persentase infeksi akar tertinggi pada agroforestri berbasis sengon 50%, sedangkan paling rendah
monokultur Porang 24%
Respon Pertumbuhan dan Produksi Jagung Manis (Zea mays L) Terhadap Pemberian Pupuk Organik Cair Urin Kelinci dan Pupuk Fosfat
No full textProduktifitas jagung manis di Indonesia masih belum optimal. Produktifitas jagung sebesar 8,13 ton/ha sedangkan potensinya 14-18 ton/ha. Peningkatan produktivitas jagung manis dapat dilakukan melalui pemberian pupuk organik. Pupuk organik cair urin kelinci dapat meningkatkan hasil tanaman jagung manis. Sedikit literatur yang mengkombinasikan urin kelinci dengan keong mas. Tujuan penelitian ini adalah untuk mengkaji pengaruh pupuk organic cair urin kelinci dan fosfat terhadap pertumbuhan dan produksi jagung manis. Penelitian dilakukan dengan menggunakan Rancangan Acak Kelompok (RAK) faktorial dengan 2 faktor dan diulang 3 kali. Faktor pertama ialah pupuk organik cair urin kelinci (K) yang terdiri dari 4 taraf yaitu: K1= urin kelinci, K2= urin kelinci + keong mas, K3= urin kelinci + keong mas + daun pepaya, K4= urine kelinci + keong mas + nanas. Faktor kedua ialah pupuk fosfat (P) yang terdiri dari 4 taraf, yaitu: P1= Tanpa fosfat, P2= SP-36, P3= rock fosfat. Hasil penelitian menunjukkan bahwa interaksi pupuk organik cair urine kelinci dan fosfat berpengaruh nyata terhadap tinggi tanaman, diameter batang, berat tongkol dengan klobot, dan berat tongkol tanpa klobot. Perlakuan yang terbaik dalam meningkatkan pertumbuhan dan produksi adalah K1P2 (urin kelinci + SP-36)
Soil Chemical Properties and Microbial Biomass Respond during Land Use Change
Get PDFIn Ponorogo, East Java, Indonesia, pine-based agroforestry was mostly converted to
sengon-based agroforestry before partly converted to monoculture. Land-use changes during the
process affect soil properties. There is few information about the effects of land-use changes
from pine-based agroforestry systems to sengon-based agroforestry systems and monocultures
on soil chemical properties and microbial biomass. The aims of this study were to analyze the
response of soil chemical properties and microbial biomass to land use changes in latosol
Indonesian. Samples from three land-use changes, i. e. pine-based agroforestry, sengon-based
agroforestry and monoculture were analyzed. Soil samples were taken at 2 depths, i. e. 0-30 cm
and 30-60 cm. As the results, it was found that the land-use change affected the chemical
properties and microbial biomass of the soil. Land-used change from pine-based agroforestry to
sengon-based agroforestry have no significant effect on pH, organic carbon, available P, and
microbial biomass in 0-30 cm depth. Meanwhile, in 30-60 cmdepth, microbial biomass in
sengon-based agroforetry was higher than that in pine-based agroforestry. The pine-based
agroforestry which was converted into monoculture, reduced the soil organic carbon and
available P concentration, contrast with the soil microbial biomass which was increased in 0-30
cm depth. The highest soil microbial biomass was found in sengon-based agroforestry in 30-60
cm depth. The conclusion of this study implicated that land use in monoculture decreased soil
fertility. Therefore, conversion of pine-based agroforestry to monoculture is full of risks
Pertumbuhan, Klorofil dan Bintil Akar Kedelai (Glycine Max L.) Dipengaruhi Kompos Limbah Kayu Putih dan Pupuk
Get PDFSoybean production on vertisol is still low and the amount of cajeput waste is much. The purpose of this study was to
determine the effect of compost from cajeput waste and inorganic fertilizer to increase growth, chlorophyll and root nodules of
soybean. This research was conducted using a randomized block design with three replications. The treatment were without
fertilizer, 150 NPK, compost, compost + 50 NPK, compost + 100 NPK and compost + 150 NPK. The results showed that the
compost increased stover dry weight, the number of branches, the amount of chlorophyll and root nodules soybean than
without fertilizer, respectively 116.31%, 52.00%, 22.05% and 30.48%. Compost combined with NPK fertilizer can increase plant
height but reduce stover dry weight. The highest stover dry weight was 7.16 g/plant on treatment of compost alone
Pengaruh pengapuran dan dosis pupuk fosfat terhadap pertumbuhan dan hasil tanaman kacang tanah (Arachis hypogeal L.) varietas macan
Get PDFTujuan Percobaan adalah untuk mengetahui sejauh mana Pengaruh Pengapuran dan dosis Pupuk Fosfat terhadap Pertumbuhan dan hasil tanaman Kacang Tanah. Percobaan dilaksanakan dilahan yang letaknya di Desa Tonatan ,kecamatan Ponorogo,Kabupaten Ponorogo.dengan ketinggian temapt 115 meter dpl dengan pH tanah 6,2 dan jenis tanah Latosol. Rancangan yang dipergunakan adalah Rancangan Acak Kelompok ( RAK ) yang terdiri dari 2 faktor dengan 12 perlakuan kombinasi dan diulang 3 kali , yaitu : Faktor Pertama terdiri dari 3 level , yaitu : ( K0 ) tanah tanpa diberi kapur , ( K1 ) tanah diberi kapur 1 ton / ha dan ( K2) tanah yang diberi kapur 2 ton / ha. Sedang Faktor yang kedua terdiri dari 4 level yaitu : ( P0) tanaman tanpa dipupuk TSP, ( P1) tanaman diberi pupuk 50 kg TSP / ha, ( P2 ) tanaman dipupuk 100 kg TSP / ha , dan ( P3 )tanaman dipupuk 150 kg TSP / ha. Hasil Penelitian menunjukkan bahwa pengapuran dan pemberian pupuk TSP mempunyai pengaruh interaksi terhadap tinggi tanaman , jumlah daun dan cabang , berat ose , jumlah dan berat polong serta berat brangkasan . Perlakuan yang baik hasilnya dicapai pada perlakuan K2P2 yaitu perlakuan pengapuran 2 ton / ha, dan tanaman dipupuk TSP 100 kg / ha. Pada perlakuan pengapuran 2 ton / ha dan tanaman dipupuk TSP 100 kg / ha, memberikan hasil tertinggi yaitu 2,03 ton / ha . Untuk hasil yang terendah dicapai pada perlakuan K0P0 yaitu tanpa pemberian kapur pada tanah dan tanpa pemberian pupuk TSP yang hanya mencapai 1,28 ton / ha
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