6 research outputs found
STAT-3-Aktivierung in humanen endometrialen Primärzellkulturen durch LIF und IL-11
No full textLeukemia inhibitory factor (LIF) and Interleukin (IL) 11, two cytokines of the IL-6 Family, have been identified as regulatory molecules during blastocyst implantation in mice. IL-11 knock out (k.o.) mice, as well as LIF Rß k.o. mice demonstrated fertility problems. Thus the hypothesis was generated that IL-11 and LIF might be important for human fertility as well. IL-6 type cytokines use the JAK/STAT Pathway for signal transduction. To demonstrate that IL-11 and LIF influence human endometrial cells, we incubated different groups of endometrial cells either with IL-11 or LIF. Then non-activated and activated phosphorylated (p) STAT 3 was detected in these cells and in control-groups. To show the response of the human endometrium to these cytokines in different cell populations and different stages of the menstrual cycle epithelial cells, stromal cells and in vitro decidualised stromal cells were employed. No activation of STAT 3 was seen in human epithelial cells via IL-11, nor LIF, which was probably caused by the low cell count, a methodical problem. IL-11 was shown to activate STAT 3 mainly in in vitro decidualised cells. LIF seems to activate STAT 3 in stromal cells during the entire hormonally influenced parts of the menstrual cycle. But also for LIF the strongest activation was detected in in vitro decidualised cells. Our results demonstrate that signal transduction for these cytokines in human endometrial cells occurs via the JAK/STAT Pathway. The main target cells for these cytokines are the decidual cells, which play a significant role for implantation and therefore for fertility
STAT-3-Aktivierung in humanen endometrialen Primärzellkulturen durch LIF und IL-11
No full textLeukemia inhibitory factor (LIF) and Interleukin (IL) 11, two cytokines of the IL-6 Family, have been identified as regulatory molecules during blastocyst implantation in mice. IL-11 knock out (k.o.) mice, as well as LIF Rß k.o. mice demonstrated fertility problems. Thus the hypothesis was generated that IL-11 and LIF might be important for human fertility as well. IL-6 type cytokines use the JAK/STAT Pathway for signal transduction. To demonstrate that IL-11 and LIF influence human endometrial cells, we incubated different groups of endometrial cells either with IL-11 or LIF. Then non-activated and activated phosphorylated (p) STAT 3 was detected in these cells and in control-groups. To show the response of the human endometrium to these cytokines in different cell populations and different stages of the menstrual cycle epithelial cells, stromal cells and in vitro decidualised stromal cells were employed. No activation of STAT 3 was seen in human epithelial cells via IL-11, nor LIF, which was probably caused by the low cell count, a methodical problem. IL-11 was shown to activate STAT 3 mainly in in vitro decidualised cells. LIF seems to activate STAT 3 in stromal cells during the entire hormonally influenced parts of the menstrual cycle. But also for LIF the strongest activation was detected in in vitro decidualised cells. Our results demonstrate that signal transduction for these cytokines in human endometrial cells occurs via the JAK/STAT Pathway. The main target cells for these cytokines are the decidual cells, which play a significant role for implantation and therefore for fertility
Die Coronavirus-Pandemie : gesellschaftliche, ökonomische und politische Folgen für Europa
No full textDie enorme Tragweite der omnipräsenten Pandemie
sowie wissenschaftliche Erhebungen implizieren,
dass dies nicht die letzte Pandemie ist, mit der sich
die Welt konfrontiert sehen wird und erfordern eine
sorgfältige Reflexion der gesammelten Erfahrungen,
Analysen und Erkenntnisse
Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins
No full text© 2020, The Author(s). The plasma membrane of eukaryotic cells is asymmetric with respect to its phospholipid composition. Analysis of the lipid composition of the outer leaflet is important for understanding cell membrane biology in health and disease. Here, a method based on cyclodextrin-mediated lipid exchange to characterize the phospholipids in the outer leaflet of red blood cells (RBCs) is reported. Methyl-α-cyclodextrin, loaded with exogenous lipids, was used to extract phospholipids from the membrane outer leaflet, while delivering lipids to the cell to maintain cell membrane integrity. Thin layer chromatography and lipidomics demonstrated that the extracted lipids were from the membrane outer leaflet. Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species. Fluorescence quenching confirmed the delivery of exogenous lipids to the cell outer leaflet. The developed lipid exchange method was then used to remove phosphatidylserine, a phagocyte recognition marker, from the outer leaflet of senescent RBCs. Senescent RBCs with reconstituted membranes were phagocytosed in significantly lower amounts compared to control cells, demonstrating the efficiency of the lipid exchange process and its application in modifying cell–cell interactions
Strategies to increase the service life of concrete bridge decks
No full textTitle from PDF title page (viewed on March 27, 2017)."FHWA-OR-RD-17-10"--Technical report documentation page.This archived document is maintained by the Oregon State Library as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Includes bibliographical references (pages 97-108).Sponsored by Oregon Department of Transportation, Research Section; Federal Highway Administration SPR 780Mode of access: Internet from the Oregon Government Publications Collection.Text in English.Final repor
Global, regional, and national incidence of six major immune-mediated inflammatory diseases : findings from the global burden of disease study 2019
No full textDATA SHARING STATEMENT : Data used for the analyses are publicly available from the Institute of Health Metrics and Evaluation (http://www.healthdata.org/; http:// ghdx.healthdata.org/gbd-results-tool).BACKGROUND : The causes for immune-mediated inflammatory diseases (IMIDs) are diverse and the incidence trends of IMIDs from specific causes are rarely studied. The study aims to investigate the pattern and trend of IMIDs from 1990 to 2019. METHODS : We collected detailed information on six major causes of IMIDs, including asthma, inflammatory bowel disease, multiple sclerosis, rheumatoid arthritis, psoriasis, and atopic dermatitis, between 1990 and 2019, derived from the Global Burden of Disease study in 2019. The average annual percent change (AAPC) in number of incidents and age standardized incidence rate (ASR) on IMIDs, by sex, age, region, and causes, were calculated to quantify the temporal trends. FINDINGS : In 2019, rheumatoid arthritis, atopic dermatitis, asthma, multiple sclerosis, psoriasis, inflammatory bowel disease accounted 1.59%, 36.17%, 54.71%, 0.09%, 6.84%, 0.60% of overall new IMIDs cases, respectively. The ASR of IMIDs showed substantial regional and global variation with the highest in High SDI region, High-income North America, and United States of America. Throughout human lifespan, the age distribution of incident cases from six IMIDs was quite different. Globally, incident cases of IMIDs increased with an AAPC of 0.68 and the ASR decreased with an AAPC of −0.34 from 1990 to 2019. The incident cases increased across six IMIDs, the ASR of rheumatoid arthritis increased (0.21, 95% CI 0.18, 0.25), while the ASR of asthma (AAPC = −0.41), inflammatory bowel disease (AAPC = −0.72), multiple sclerosis (AAPC = −0.26), psoriasis (AAPC = −0.77), and atopic dermatitis (AAPC = −0.15) decreased. The ASR of overall and six individual IMID increased with SDI at regional and global level. Countries with higher ASR in 1990 experienced a more rapid decrease in ASR. INTERPRETATION : The incidence patterns of IMIDs varied considerably across the world. Innovative prevention and integrative management strategy are urgently needed to mitigate the increasing ASR of rheumatoid arthritis and upsurging new cases of other five IMIDs, respectively.The Global Burden of Disease Study is funded by the Bill and Melinda Gates Foundation. Support from Scientific Research Fund of Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital; Shaqra University; the School of Pharmacy, University of Botswana; the Indian Council of Medical Research (ICMR); an Australian National Health and Medical Research Council (NHMRC) Investigator Fellowship; the Italian Center of Precision Medicine and Chronic Inflammation in Milan; the Department of Environmental Health Engineering of Isfahan University of Medical Sciences, Isfahan, Iran; National Health and Medical Research Council (NHMRC), Australia; Jazan University, Saudi Arabia; the Clinician Scientist Program of the Clinician Scientist Academy (UMEA) of the University Hospital Essen; AIMST University, Malaysia; the Department of Community Medicine, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India; a Kornhauser Research Fellowship at The University of Sydney; the National Research, Development and Innovation Office Hungary; Taipei Medical University; CREATE Hope Scientific Fellowship from Lung Foundation Australia; the National Institute for Health and Care Research Manchester Biomedical Research Centre and an NIHR Clinical Lectureship in Respiratory Medicine; Kasturba Medical College, Mangalore and Manipal Academy of Higher Education, Manipal; Author Gate Publications; the Cleveland Clinic Foundation and Nassau University Medical center; the Italian Ministry of Health (RRC); King Abdulaziz University (DSR), Jeddah, and King Abdulaziz City for Science & Technology (KACSAT), Saudi Arabia, Science & Technology Development Fund (STDF), and US-Egypt Science & Technology joint Fund: The Academy of Scientific Research and Technology (ASRT), Egypt; partially supported by the Centre of Studies in Geography and Spatial Planning; the International Center of Medical Sciences Research (ICMSR), Islamabad Pakistan; Ain Shams University and the Egyptian Fulbright Mission Program; the Belgian American Educational Foundation; Health Data Research UK; the Spanish Ministry of Science and Innovation, Institute of Health Carlos III, CIBERSAM, and INCLIVA; the Clinical Research Development Unit, Imam Reza Hospital, Mashhad University of Medical Sciences; Shaqra University; Saveetha Institute of Medical and Technical Sciences and SRM Institute of Science and Technology; University of Agriculture, Faisalabad-Pakistan; the Chinese University of Hong Kong Research Committee Postdoctoral Fellowship Scheme; the institutional support of the Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Egypt; the European (EU) and Developing Countries Clinical Trials Partnership, the EU Horizon 2020 Framework Programme, UK-National Institute for Health and Care Research, the Mahathir Science Award Foundation and EU-EDCTP.http://www.thelancet.comam2024School of Health Systems and Public Health (SHSPH)SDG-03:Good heatlh and well-bein
