112,202 research outputs found
Opportunità di gestione del rischio di insolvenza attraverso il processo di definizione del debito
No full textMaternal perception of emotional expressiveness. A cross-cultural comparison
No full textThe parents' perception of children is a crucial aspect involving the relationship between caregiver and children (Stern, 2007) that represents an important area of investigation during the clinical assessment in developmental age. Although maternal perception is conceived as a mixture of objective and subjective judgments (Regan et al., 1974; Taylor e Koivumaki, 1976; Goldberg, 1981), influenced by multiple factors, such as the personal experience with the baby and parents' own personality, little is known about the influence of the cultural context. The present research explored the cultural influence on the parental perspectives by means of a cross-cultural approach. In particular, this study investigated parents' perception of different ethnic groups and compared them with the direct emotional expression of children. Participants were 165 mothers and 165 children, aged between 6 and 18 years, divided in three samples: Italians, African and Latin-American. Parents' perception was measured by means of the Child Behavior Checklist (Achenbach, 1991), whereas children emotional expression was derived by spontaneous narratives to the Roberts-2 (Roberts e Gruber, 2005). Results showed cultural differences in CBCL profile, related to the ethnic group and to gender, as well as in the emotions expressed in the test Robert-2
Platform work and economic insecurity in Italy
No full textUsing representative survey data from Italy, this study investigates the levels and sources of economic security amongst platform workers relative to other labour force participants. Platform workers face greater economic insecurity relative to all other occupation groups, and a rate of economic insecurity that is not significantly different from that of unemployed adults. Higher levels of insecurity are not primarily channelled through lower incomes; instead, higher rates of insecurity persist when accounting for family incomes, suggesting that other dimensions of precarity associated with platform work matter as much as income differences in shaping economic insecurity
Histones post-translational modifications in colon cancer cell line determined by HPLC-ESI-MS and Maldi-Tof for the characterization of deacetylase inhibitors
No full textThe nucleosome is the basic structural unit of eukariotic chromosomes and consists of a DNA molecule associated with a histone octamer comprised of pairs of the core histones H2A, H2B, H3 and H4. Linker DNA and histone1 to form chromatin join the nucleosomes. Histones play an important role in transcription, DNA replication, DNA repair and recombination. Post-translational modifications of specific residues in the core histones (acetylation, methylation, phosphorylation, etc.) have been demonstrated to be critical to their regulatory function. In particular acetylation is a very specific phenomenon with various isoforms playing distinct roles. Increased acetylation is generally correlated with transcriptionally active or poised genes. Histone deacetylases’ inhibitors (short chain fatty acids, such as sodium butyrate, hydroxamic acids such as thrichostatin A) have been described as potential cancer therapeutics in a variety of preclinical studies1. Inhibitors’ treatment of cells resulted in the increase of highly acetylated isoforms of the histones, which affect gene expression, cell differentiation and apoptosis.
It is here described the application of reversed-phase high-pressure liquid chromatography under gradient conditions and mass spectrometry (LC-ESI-MS) to analyse global modification levels of core histones. The LC-MS method was optimised using histones extracted from HT 29 colon cancer cell line2. Histones eluted from the column were detected with Q-Tof and ion trap mass spectrometers with an electrospray source put in parallel and UV detection at 214 nm.
These methods were then applied to the characterisation of changes in histone modification in HT29 treated with histone deacetylase inhibitors such as valproic acid, sodium butyrate and 9-hydroxystearic acid. Acetylation were found located in the H4 histone tail by MALDI-Tof analysis of tryptic and arginase peptide digests.
1Rasheed WK, Johnstone RW, Prince H,Histone deacetylase inhibitors in cancer therapy Expert Opin Investig Drugs. 2007 May;16(5):659-78.
2Naldi M, Andrisano V, Fiori J, Calonghi N, Pagnotta E, Parolin C, Pieraccini G,Masotti L. Histone proteins determined in a human colon cancer by high-performance liquid chromatography and mass spectrometry. J Chromatogr A. 2006 Sep 29;1129(1):73-81
Platform work and economic insecurity in Italy
No full textUsing representative survey data from Italy, this study investigates the levels and sources of economic security amongst platform workers relative to other labour force participants. Platform workers face greater economic insecurity relative to all other occupation groups, and a rate of economic insecurity that is not significantly different from that of unemployed adults. Higher levels of insecurity are not primarily channelled through lower incomes; instead, higher rates of insecurity persist when accounting for family incomes, suggesting that other dimensions of precarity associated with platform work matter as much as income differences in shaping eco-nomic insecurity
Benefits of the multi-modality formulation in hydrogen supply chain modelling
No full textHydrogen is recognized as a key element of future low-carbon energy systems. For proper integration, an adequate delivery infrastructure will be required, to be deployed in parallel to the electric grid and the gas network. This work adopts an optimization model to support the design of a future hydrogen delivery infrastructure, considering production, storage, and transport up to demand points. The model includes two production technologies, i.e., steam reforming with carbon capture and PV-fed electrolysis systems, and three transport modalities, i.e., pipelines, compressed hydrogen trucks, and liquid hydrogen trucks. This study compares a multi-modality formulation, in which the different transport technologies are simultaneously employed and their selection is optimized, with a mono-modality formulation, in which a single transport technology is considered. The assessment looks at the regional case study of Lombardy in Italy, considering a long-term scenario in which an extensive hydrogen supply chain is developed to supply hydrogen for clean mobility. Results show that the multi-modality infrastructure provides significant cost benefits, yielding an average cost of hydrogen that is up to 11% lower than a mono-modality configuration
author-bios-SRD-19-0063.R1 – Supplemental material for The Network Structure of Police Misconduct
No full textSupplemental material, author-bios-SRD-19-0063.R1 for The Network Structure of Police Misconduct by George Wood, Daria Roithmayr and Andrew V. Papachristos in Socius</p
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