84 research outputs found
Phytochemical, antimicrobial and mast cell stabilizing activity of ethanolic extract of Solanum trilobatum Linn. leaves
No full textAims: Solanum trilobatum Linn., (Solanaceae) is one of the most widely used plants as food supplement in southern
part of India and some parts of Southeast Asia. This plant is traditionally used for the treatment of respiratory illness. In
animal studies, the extract of S. trilobatum showed significant antimicrobial, hepatoprotective and anticancer activities.
The complete phytochemical profile, antimicrobial and mast cell stabilizing activities of S. trilobatum remains unclear.
This study tests the antimicrobial, antihistaminic and mast cell stabilizing activities of ethanolic extract of leaves of S.
trilobatum (EEST).
Methodology and results: The phytochemical test was carried out using chemical and instrumental [Gas
Chromatography Mass Spectrometry (GC-MS)] analytical methods. Antimicrobial effect of EEST was tested against
Streptococcus pneumonia, Escherichia coli and Staphylococcus aureus. Intestinal mesentery of Sprague Dawley (SD)
rats was used to study the peritoneal mast cell stabilization activity of EEST. The rat intestinal mesentery was exposed
to 50, 100, 200, 300, 400 and 600 ?g/mL of EEST and the peritoneal mast cell stabilization activity was compared with
that of standards (pheniramine 20 ?g/mL and ketotifen 20 ?g/mL). The phytochemical test showed the presence of
carbohydrates, saponins, flavonoids, alkaloids, tannins and phenolic compounds. GC-MS analysis indicated the
presence of 45 fragmented compounds which included epoxylinalol, himachalol, illudol, epibuphanamine, baimuxinal
and edulan IV. EEST exhibited antimicrobial activity at 10 mg/mL against S. aureus, S. pneumonia. Significant mast cell
stabilizing activity was observed from the dose of 100 ?g/mL to 600 ?g/mL.
Conclusion: Ethanolic extract of leaves of S. trilobatum possess significant antimicrobial and antihistaminic activity
New Editorial Board (2017-18) – Widening the scope of the “Journal of Pharmaceutical Negative Research (JPNR)” into biomedical research
No full text
Behavioral, biochemical, and pathological alterations induced by electromagnetic radiation in Sprague-Dawley rats
No full textAntidiabetic Activity of Polyherbal Formulation in Streptozotocin – Nicotinamide Induced Diabetic Wistar Rats
No full textABSTRACTGlycosmis pentaphylla, Tridax procumbens, and Mangifera indica are well-known plants available throughout India and they are commonly used for the treatment of various diseases including diabetes mellitus. The antidiabetic activity of the individual plant parts is well known, but the synergistic or combined effects are unclear. The concept of polyherbalism has been highlighted in Sharangdhar Samhita, an Ayurvedic literature dating back to 1300AD. Polyherbal formulations enhance the therapeutic action and reduce the concentrations of single herbs, thereby reducing adverse events. The aim of the present study is to formulate a polyherbal formulation and evaluate its antidiabetic potential in animals. The polyherbal formulation was formulated using the ethanol extracts of the stem bark of G. pentaphylla, whole plant of T. procumbens, and leaves of M. indica. The polyherbal formulation contains the ethanol extracts of G. pentaphylla, T. procumbens, and M. indica in the ratio of 2:2:1. The quality of the finished product was evaluated as per the World Health Organization’s guidelines for the quality control of herbal materials. The quality testing parameters of the polyherbal formulation were within the limits. Fingerprint analysis of the polyherbal formulation showed effective separation at 366nm, and it revealed that the active compound present in the polyherbal formulation and the active compounds present in all the three extracts were the same. The acute toxicity studies of the polyherbal formulation did not show any toxic symptoms in doses up to 2000mg/kg over 14days. The oral antidiabetic activity of the polyherbal formulation (250 and 500mg/kg) was screened against streptozotocin (50mg/kg; i.p.)+nicotinamide (120mg/kg; i.p.) induced diabetes mellitus in rats. The investigational drug was administered for 21 consecutive days, and the effect of the polyherbal formulation on blood glucose levels was studied at regular intervals. At the end of the study, the blood samples were collected from all the animals for biochemical estimation, and the animals were sacrificed and the liver and pancreatic tissues were collected for histopathologic analysis. Polyherbal formulation showed significant antidiabetic activity at 250 and 500mg/kg, respectively, and this effect was comparable with that of glibenclamide. The antidiabetic activity of polyherbal formulation is supported by biochemical and histopathologic analysis
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