122,751 research outputs found
Placental expression of PAPPA, PAPPA-2 and PLAC-1 in pregnacies is associated with FGR
No full textFetal growth restriction (FGR) is a gynecological disorder of varying etiology. In the present study, an expression analysis of pregnancy-associated plasma protein A (PAPPA), pregnancy-associated plasma protein A2 (PAPPA2) and placenta-specific-1 (PLAC-1) was conducted in pregnancies with FGR and control pregnancies. Placental tissues were collected from pregnancies with FGR (n=16) and control pregnancies (n=16) and the expression of the genes of interest was examined by qPCR. The mean expression levels of PAPPA and PAPPA2 were significantly lower (P<0.001) in placental tissues from FGR pregnancies compared with tissues from healthy subjects, whereas the opposite pattern was observed for PLAC-1 (P<0.001). PAPPA and PLAC-1 expression in FGR and control subjects correlated with birth weight (P<0.001). The findings suggest a possible pathophysiological link between the development of FGR and the expression of PAPPA, PAPPA2 and PLAC-1. © 2018 Spandidos Publications. All rights reserved
Removal of <i>Mcs5c</i> TCE copies results in decreased <i>Pappa</i> expression <i>in vitro</i>.
No full text(A) CRISPR guides targeting the TCE were transfected into LA7 cells, and clones were screened for removal of the target region. Nine positive clones were assessed for remaining TCE copy number via qPCR, standardized to a non-targeted region within the Pappa gene. Copy number in wild-type LA7 cells (n = 4 independent cultures) was also assessed, and results were normalized to diploid MECs. (B) Interaction frequency (IF, y-axis) was calculated in select positive clones (n = 3) and WT LA7 cells between the TCE and Pappa bait regions P4-1 and P3-3. The IF for a positive control region, two nearby BglII fragments, is shown for reference. (C) Pappa expression in positive clones and WT LA7 cells (n = 6) was analyzed via qPCR and standardized to Tbp expression. (D) A scatterplot of Pappa expression and Mcs5c TCE copy number demonstrate a statistically significant positive correlation between the two (Pearson correlation coefficient, R, = 0.6245, n = 13, p-value = 0.0225). A linear trend line is shown (slope = 5.327). (E) WT LA7 cells were treated with 0μM (n = 4) or 1μM (n = 4) 5-aza-dC for 48hrs. Pappa expression was analyzed via qPCR and standardized to Tbp. (F) Methylation levels at Pappa CGI shore site 12 are shown for WT LA7 cells (n = 8) and CRISPR clones (n = 9). The p-value reflects Bonferroni correction. Scatterplots demonstrating negative correlations for shore site 12 methylation levels and Mcs5c TCE copy number (G) and Pappa expression (H) are shown (R = -0.8034/-0.6022, n = 13/17, p-value = 0.0009/0.011, respectively) along with linear tread lines (slope = -0.046/-0.004, respectively). For all bar graphs, p-values were obtained using the non-parametric Mann-Whitney U test, and standard error bars are shown (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001).</p
PAPPA expression in human HCC tissues of different tumor stages.
No full text<p>PAPPA mRNA expression levels in human HCC tissues (n = 52) of tumor stages I (n = 12), II (n = 19) and III (n = 21). One-way ANOVA shows a significant effect (p = 0.008) of tumor stage on PAPPA mRNA expression level.</p
Correction to: The role of intra-articular administration of Fetuin-A in post-traumatic knee osteoarthritis: an experimental study in a rat model (Journal of Experimental Orthopaedics, (2019), 6, 1, (25), 10.1186/s40634-019-0194-4)
No full textFollowing publication of the original article (Pappa et al. 2019), the authors opted to correct the middle initial of co-author Despina N. Perrea from S to N. The original article has been corrected. © 2019, The Author(s)
New technologies for territorial system recovery. The Moscow-St-Petersbourgh example
No full textPappa Rocco, Fistola Romano. New technologies for territorial system recovery. The Moscow-St-Petersbourgh example. In: NETCOM : Réseaux, communication et territoires / Networks and Communication Studies, vol. 10 n°2, août 1996. pp. 476-498
<i>In vivo</i> methylation analysis of the <i>Pappa</i> CGI and CGI shore.
No full text(A) The first exon of the Pappa gene is shown in relation to a conserved CGI (green box) and the P4-1 looping fragment (gray box). The location of the 12 shore CG dinucleotides investigated in this report are indicated and numbered, as are the regions covered by the two pre-made CGI pyrosequencing assays. The CGI assays each examined 5 CG dinucleotides within the island. (B) A scatterplot demonstrating a statistically significant negative correlation between 6 week MEC Pappa expression (x-axis) and shore methylation (y-axis; Pearson correlation coefficient, R, = -0.67, n = 18, p-value = 0.0023) is shown. Shore methylation values were obtained by averaging the absolute methylation percentages of the 6 significant shore sites (Sites 1, 3, 6–9) for each individual sample. A linear trend line is shown with the dotted line (slope = -7.88). (C) No correlation was observed between 6 week MEC Pappa expression (x-axis) and CGI methylation (y-axis; Pearson correlation coefficient, R, = 0.16, n = 18, p-value = 0.52). CGI methylation values were obtained by averaging the absolute methylation percentages of the 5 sites examined by the CGI-2 assay for each individual sample. A linear trend line is shown with the dotted line (slope = 0.544).</p
ASSIGNMENT OF THE HUMAN GENE FOR PREGNANCY-ASSOCIATED PLASMA PROTEIN-A (PAPPA) TO 9Q33.1 BY FLUORESCENCE INSITU HYBRIDIZATION TO MITOTIC AND MEIOTIC CHROMOSOMES
No full textLow levels of pregnancy-associated plasma protein A (PAPPA) during the first trimester has been suggested as a biochemical indicator of pregnancies with aneuploid fetuses. Furthermore, the complete absence of PAPPA in pregnancies associated with Cornelia de Lange syndrome (CL) has suggested a causal connection between PAPPA and the development of CL. We have assigned the locus for PAPPA to chromosome region 9q33.1 on mitotic and meiotic chromosomes by fluorescence in situ hybridization, using a 3.7-kb partial PAPPA cDNA probe
Correlation of HSC secreted PAPPA levels with NFκB activation in conditioned HCC.
No full text<p>A. Correlation of HSC-CM induced NFκB activity in HCC cells (relative to NFκB activity in cells stimulated with control medium) with PAPPA levels in HSC-CM (n = 15). B. HCC cells were incubated with recombinant human PAPPA protein (PAPPA) either in CM from HCSs from 2 different human donors (CM1 and CM2) or control medium (ctr.). Furthermore, cells were stimulated with CM1, CM2 or control medium alone. After 4h stimulation, cellular extracts were analyzed with Western blot analysis for phosphorylated p65 and IkB-alpha. Analysis of actin expression demonstrated equal loading.</p
The Master Builders: LAIRAH Research on Good Practice in the Construction of Digital Humanities Projects
No full textThis paper describes the results of research carried out during the LAIRAH (Log analysis of Internet Resources in the Arts and Humanities) project (http://www.ucl.ac.uk/slais/circah/lairah/) which is based at UCL’s School of Library Archive and Information Studies. It was a fifteen month study (reporting in October 2006) to discover what influences the long-term sustainability and use of digital resources in the humanities through the analysis and evaluation of real-time use. At Digital Humanities 2006 we reported on the early stages of the project, in which we carried out deep log analysis of the AHDS and Humbul portals to determine the level of use of digital resources. (Warwick et al. 2006) This proposal will discuss the results of the final phase of the research in which we examined digital resources from the point of view of those who designed and built them. We aimed to discover whether there were common characteristics and elements of good practice linking resources that are well- used. Numerous studies have been carried out into the information needs and information seeking practices of humanities scholars (Barrett, (2005) Talja and Maula (2003), Herman (2001) and British Academy, (2005)). However, our research is original because it surveys the practices of those who produce digital humanities resources. We also based the selection of our projects on deep log analysis: a quantitative technique which has not previously been applied to digital humanities resources to ascertain real usage levels of online digital resources. Method: We selected a sample of twenty one publicly funded projects with varying levels of use, covering different subject disciplines, to be studied in greater depth. We classified projects as well-used if the server log data from the Arts and Humanities Data Service (AHDS) and Humbul portals showed that they had been repeatedly and frequently accessed by a variety of users. We also mounted a questionnaire on these sites and asked which digital resources respondents found most useful. Although most nominated information resources, such as libraries, archives and reference collections for example the eDNB, three UK publicly funded research resources were mentioned, and thus we added them to the study. We also asked representatives of each AHDS centre to specify which resources in their collections they believed were most used. In the case of Sheffield University the logs showed that a large number of digital projects accessed were based at the Humanities Research Institute. We therefore conducted interviews about the HRI and its role in fostering the creation of digital humanities resources. The selected projects were studied in detail, including any documentation and reports that could be found on the project’s website. We also interviewed a representative of the project, either the principal investigator or a research assistant. Results: Institutional context: The majority of projects that we interviewed had been well supported in technical terms, and this had undoubtedly aided the success of the project, especially where it was associated with a centre of digital humanities excellence such as the Centre for Computing in the Humanities at Kings College London or the HRI at Sheffield. Critical mass aided the spread of good practice in the construction and use of digital resources in the humanities. Where a university valued such activities highly they tended to proliferate. More junior members of staff were inspired to undertake digital humanities research by the success of senior colleagues and early adopters respected for their traditional and digital research. Unfortunately such critical mass is relatively rare in UK universities and some PIs reported that their digital resource was not understood or valued by their departments, and thus their success had not lead to further digital research. Staffing: PIs also stressed how vital it had been to recruit the ideal RAs. These were however relatively difficult to find, as they had to have both disciplinary research expertise and good knowledge of digital techniques. Most RAs therefore required training, which many PIs often found lacking or of poor quality. A further frustration was the difficulty of finding funding to continue research, this meant that an expert RA might leave, necessitating further training of a new employee if the project was granted future funding. Dissemination: The strongest correlation between well-used projects and a specific activity was in the area of dissemination. In all the projects studied, staff had made determined efforts to disseminate information as widely as possible. This was a new challenge for many humanities academics, who were more used to writing books, marketed by their publishers. This might include giving papers at seminars and conferences both within the subject community and the digital humanities domain; sending out printed material; running workshops, and in the most unusual instance, the production of a tea-towel! User contact: Very few projects maintained contact with their users or undertook any organised user testing, and many did not have a clear idea how popular the resource was or what users were doing with it. However, one of the few projects that had been obliged to undertake user surveys by its funders was very well-used, and its PI had been delighted at the unexpected amount and range of its use. Another project came to the belated realisation that if it had consulted users the process of designing the resource would have been simpler and less demanding. Documentation: Few of the projects kept organised documentation, with the exception of those in archaeology, linguistics and archival studies, where such a practice is the norm in all research. Most projects had kept only fragmentary, internal documents, many of which would not be comprehensible to someone from outside. Documentation could also be difficult to access, with only a small minority of projects making this information available from its website. This is an important omission since documentation aids reuse of resources, and also provides vital contextual information amount its contents and the rationale for its construction that users need to reassure them about the quality of the resource for academic research. Sustainability: Another area of concern was the issue of sustainability. Although the resources were offered for deposit with the AHDS, few PIs were aware that to remain usable, both the web interface and the contents of the resource would require regular updating and maintenance, since users tend to distrust a web page that looks outdated. Yet in most cases no resources were available to perform such maintenance, and we learnt of one ten year old resource whose functionality had already been significantly degraded as a result. Conclusion and recommendations Well-used projects do therefore share common features that predispose them to success. The effect of institutional and disciplinary culture in the construction of digital humanities projects was significant. We found that critical mass was vital, as was prestige within a university or the acceptance of digital methods in a subject. The importance of good project staff and the availability of technical support also proved vital. If a project as to be well-used it was also essential that information about it should be disseminated as widely as possible. Even amongst well-used projects, however we found areas that might be improved, these included organised user testing, the provision of and easy access to documentation and the lack of updating and maintenance of many resources. Recommendations: Documentation: * Projects should keep documentation and make it available from the project web site, making clear the extent, provenance and selection methods of materials for the resource. * Funding bodies might consider making documentation a compulsory deliverable of a funded project. * Discussions could be held between relevant stakeholders and the funding bodies, with the aim of producing an agreed documentation template. This should specify what should be documented and to what level of detail. Users: * Projects should have a clear idea of whom the expected users might be; consult them as soon as possible and maintain contact through the project via a dedicated email list , website feedback or other appropriate method * They should carry out formal user surveys, software and interface tests and integrate the results into project design. * Applicants for funding should show that they have consulted documentation of other relevant projects and discuss what they have learnt from it in their case for support. The results of such contact could then be included in the final report as a condition of satisfactory progress. Management: * Projects should have access to good technical support, ideally from a centre of excellence in digital humanities. * Projects should recruit staff who have both subject expertise and knowledge of digital humanities techniques, then train them in other specialist techniques as necessary. * Funding bodies might consider requiring universities to offer more training for graduate students and RAs in digital humanities techniques. Sustainability: * Ideally projects should maintain and actively update the interface, content and functionality of the resource, and not simply archive it with a data archive such as the AHDS. However this is dependent on a funding model which makes this possible. Dissemination: * Disseminate information about itself widely, both within its own subject domain and in digital humanities. * Information should be disseminated widely about the reasons for user testing and its benefits, for example via AHRC/AHDS workshops. Projects should be encouraged to collaborate with experts on user behaviour. Acknowledgements: This project was funded by the Arts and Humanities Research Council ICT Strategy Scheme. We would also like to thank all of out interviewees for agreeing to talk to us. Bibliography * Barrett, A. The Information Seeking Habits of Graduate Student Researchers in the Humanities. The Journal of Academic Librarianship 31.4 (2005): 324-331. * British Academy. E-resources for Research in the Humanities and Social Sciences - A British Academy Policy Review section 3.5 . 2005. http://www.britac.ac.uk/reports/eresources/report/sect3.html#part5 * Herman, E. End-users in Academia: Meeting the Information Needs of University Researchers in an Electronic Age Part 2 Innovative Information-accessing Opportunities and the Researcher: User Acceptance of IT-based Information Resources in Academia. Aslib Proceedings. 2001. 431-457. * Talja, S., and H. Maula. Reasons for the Use and Non-use of Electronic Journals and Databases - A Domain Analytic Study in Four Scholarly Disciplines. Journal of Documentation 59.6 (2003): 673-691. * Warwick, C., M. Terras, P. Hungtington, and N. Pappa. If You Build It Will They Come? The LAIRAH Survey of Digital Resources in the Arts and Humanities. Paper presented at Digital Humanities 2006, Paris Sorbonne, 5-9 July 2006. 2006
A Multi-Language Comparison of Influences on Author Verification using Character N-Grams
No full textWe create a new multi-language corpus for author verification based on Wikipedia talkpages, and evaluate the influence that differences in topic and time have on character n-gram author profiles. Topic alignment between two texts is found to increase author verification precision, and an authors writing style is found to change over time, but not more significantly after 3 years than after 1 year.Information ArchitectureWISElectrical Engineering, Mathematics and Computer Scienc
- …
