1,742,755 research outputs found
La pappa reale: aspetti tecnici ed analitici
No full textSono stati affrontati l'utilizzo di tecniche analitiche innovative (NIR e NMR) per la valutazione della qualità della pappa reale
Jinghan Li (Alex)_SURF2025_Evaluation of CRISPR/Cas9-Based Gene Fragment Deletion in Primary Human Chondrocytes using the Pregnancy-Associated Plasma Protein A (PAPPA) Gene as Target
No full textOsteoarthritis (OA) is the most common joint disorder, impacting more than 500 million people worldwide, and has become increasingly prominent in the past two decades. It can lead to serious health and economic burden, while there is currently no curative treatment for OA because its disease development is complex. Genetic factors are the top contributing factors of OA, and it is imperative to understand the genetic basis of OA. Pregnancy-associated plasma protein A (PAPPA) is a novel gene identified to be involved in OA development with support of genome-wide association studies (GWAS), expression quantitative trait loci (eQTL), and high-throughput chromatin conformation capture (Hi-C) data. This study used primary human chondrocytes as the model system and employed ribonucleoprotein transfection-based CRISPR/Cas9 delivery to create two double-stranded breaks in the exon 2 of the PAPPA gene in these cells. Screening of the population following transfection showed that a significant number of cells have undergone a deletion in the exon 2 of PAPPA, which will putatively generate a knock-out (KO) of the PAPPA gene. Single colonies were then isolated and expanded from the transfected population, and multiple colonies of cells with homozygous deletion of a region in PAPPA exon 2 were identified. These colonies of cells are highly valuable for functional follow-up experiments on changes in gene expression and phenotypes of chondrocytes with PAPPA KO, which will be performed subsequently. In summary, this study further verified the feasibility of generating gene fragment deletions using CRISPR/Cas9 in chondrocytes and has laid the basis for the functional studies of PAPPA as a potential key mediator of OA.
Faces and Places in Fashion: Dimitra Williams
No full textPart presentation, part Q&A, FIT's "Faces & Places in Fashion" lecture series is an opportunity to connect students and the public alike to the pulse of the fashion industry in an open and conversational setting.Dimitra Williams is a gradutate of FIT with a Bachelor of Fine Arts in Fashion Design. At FIT she specialized in knitwear and has continued to work in knits throughout her career. With experience at Opening Ceremony, Kim Haller and Brooks Brothers, Dimitra is currently spending her days at Helmut Lang as an associate designer. In March 2020, Dimitra founded MIITRA. She created MIITRA as a way to utilize the themes, individuals and experiences that fed her personal creativity andartistry. MIITRA is her way of amplifying the positive representation of the Black community in the luxury design space. To quote Miitra’s brand guide: “At MIITRA, our purpose is simple: to empower your spirit through thoughtfully designed pieces and to promote strength, confidence and self-love with every product we offer. We create investment pieces at an attainable price point with timeless appeal because everyone deserves to wear their values with pride. Our aesthetic is clean, modern and minimal so our statement messages of positivity and hope always remain the focus.
Dimitra
No full textTouchais Gilles. Dimitra. In: Bulletin de correspondance hellénique. Volume 109, livraison 2, 1985. p. 824
Applicazioni innovative della NMR per la caratterizzazione della pappa reale
No full textSviluppo di un metodo alternativo per caratterizzare la qualità della pappa reale e provenienza da una determinata zona geografica. La tecnica NMR verrà confrontata con i metodi di analisi classica sia per valutarne le potenzialità sia per l'ottenimento di informazioni complementari
Dimitra
No full textPariente Anne. Dimitra. In: Bulletin de correspondance hellénique. Volume 116, livraison 2, 1992. p. 915
Dimitra
No full textPariente Anne. Dimitra. In: Bulletin de correspondance hellénique. Volume 114, livraison 2, 1990. p. 796
Women’s empowerment and collective action
No full textDimitra publishes a bi-annual newsletter that provides information about the activities of the project, its partner organisations and other civil society organisations in Africa. It is a knowledge-exchange tool to share Dimitra and other partners' best practices, raise rural populations' voices and raise awareness on women and youth's role in development. Best practices and updated news about FAO-Dimitra and its partners are disseminated worldwide in an effort to improve visibility and stimulate exchanges
Lieber Pappa
No full textFür Lieber Pappa hat Wim Liebrand eine Anzahl offenbar völlig unzusammenhängender Bilder zusammengeschnitten, die trotzdem ein harmonisches, wenn auch in jeder Hinsicht >ungeheuerliches< Ganzes formen. Neben einigen seiner eigenen Aufnahmen gebraucht er in sehr effektiver Weise kurze Bild- und Ton-Fragmente aus Archivmaterial. die in Lieber Pappa und in ihrer Beziehung zueinander eine ganz und gar neue Bedeutung erhalten.
Wir sehen die Aufnahme von einem kleinen Stück Land aus der Vogelperspektive, >KlinikLieber Pappa, lieber Pappa< zu vernehmen ist; ein altes Schwarzweißbild, das einen nach oben blickenden Mann zeigt; Aufnahmen von sprießenden Pflanzen.
Einige Bilder tauchen immer wieder auf, das gilt auch für den Schrei >Lieber Pappa, lieber Pappa<. Das Brummen der Maschinen ist stets im Hintergrund zu hören, ein zufälliges Bruchstück eines deutschen Liedes ... Lieber Pappa ruft ein fast greifbares Gefühl des Unwohlseins und die bedrohliche Atmosphäre einer bevorstehenden Zerstörung hervor.
Die Bilder lassen uns an die technologische Macht des Menschen über die Natur, an Krieg (jeder Art) denken. In einer verborgenen, doch eindringlichen Weise konfrontiert uns Liebrand mit fundamentalen Fragen zur Verantwortlichkeit des Menschen
Dimitra (FAO)
No full textDimitra is an information and communication project of the FAO involving a network of local partners in Africa and the Near East
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