3,193 research outputs found
DMPK prevents ROS-induced cell death by assembling a HK II-Src complex on mitochondrial surface
No full textDMPK is a serine/threonine protein kinase that was initially proposed to be the cause of the most frequent adult muscular dystrophy, myotonic dystrophy 1 (DM1). Recently, it has been shown that DMPK is not the primary determinant of the DM1, but its deletion causes late onset myopathy and cardiac abnormalities in knock-out mice. Evidence present in the literature suggests a mitochondrial localization of high MW DMPK isoforms in muscle and cardiac tissue. However, to date, there is not a single association of mitochondria-anchored isoforms with the respective function of the organelle in the affected tissues. Therefore, we have examined the role of mitochondria-anchored isoform A, either by stably expressing it in cells lacking endogenous protein, or by stably silencing the endogenous one. DMPK significantly decreased levels of mitochondrial superoxide and consequently increased cell survival in prolonged serum and glucose depletion, both in SAOS-2 and rhabdomyosarcoma cells. At the molecular level we have found DMPK to interact with HK II and Src, increasing the HK II association to mitochondria. Detachment of HK II from mitochondria abolished differences in superoxide levels, while a HK II inhibitor 5-TG protected cells from death by stabilizing HK II on the OMM and by decreasing mitochondrial ROS in the absence of DMPK. Src activity was also important for HK II maintenance on OMM since its inhibition sensitized only DMPK-expressing cells to detachment of HK II. These data attribute an antiapoptotic role to DMPK due to an unprecedented link to HK II and its protective effect against mitochondrial ROS.DMPK è la serina/treonina protein kinasi, la quale è stata inizialmente proposta come la causa della più frequente distrofia muscolare negli adulti, la distrofia miotonica del tipo 1 (DM1). Recentemente si è visto che la DMPK non è la causa principale della DM1, ma la sua delezione causa miopatia ad insorgenza tardiva e anomalie cardiache nei topi knock-out. I dati presenti in letteratura attribuiscono la localizzazione mitocondriale alle isoforme ad alto peso molecolare nel muscolo e nel tessuto cardiaco. Comunque, finora non vi sono stati studi volti ad associare il ruolo delle isoforme mitocondriali della DMPK alla funzione dell’organulo nei tessuti in questione. Perciò, abbiamo deciso di esaminare il ruolo dall’isoforma A associata ai mitocondri, sia esprimendola stabilmente nelle cellule prive della DMPK endogena, sia silenziando stabilmente quella endogena. DMPK ha significativamente diminuito i livelli del superossido mitocondriale e, di conseguenza, ha aumentato la sopravvivenza delle cellule SAOS-2 e rabdomiosarcoma in deplezione di siero e glucosio. A livello molecolare, abbiamo trovato che la DMPK interagisce con HK II e Src aumentando l’associazione dell’HK II ai mitocondri. Il distacco dell’HK II dai mitocondri ha cancellato le differenze nei livelli di superossido, mentre l’inibitore dell’HK II 5-TG ha protetto le cellule dalla morte stabilizzando l’HK II sulla membrana mitocondriale esterna e diminuendo i livelli di ROS mitocondriali in assenza della DMPK. Src aveva la funzione di mantenere HK II sulla membrana mitocondriale esterna, in quanto la sua inibizione ha sensibilizzato le cellule al distacco dell’HK II solo se esprimevano la DMPK. Questo studio attribuisce un ruolo anti-apoptotico alla DMPK grazie all’interazione con HK II e la sua funzione protettiva contro i ROS di origine mitocondriale
Letter of concern from Boris Drasin, President of the Jersey Homesteads Industrial Cooperative Association
No full textJersey Homesteads (later renamed the Borough of Roosevelt) was established in the 1930s as an agro-industrial cooperative community. It was established specifically for urban Jewish garment workers, many of whome had emigrated from Europe. In this letter, Boris Drasin, a community leader who was the President of the Jersey Homesteads Industrial Cooperative Association, expresses his concerns to their management corporation (Consumers Wholesale Clothiers, Inc.) about how financial losses will impact the lives of Roosevelt's residents, three-fourths of whom depended on the garment factory for their livelihoods. He makes suggestions as to how the situation might be improved
Widespread Expression of BORIS/CTCFL in Normal and Cancer Cells
No full textThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Boris Smolar papers, undated, 1913-1985.
No full textThis collection contains materials pertaining to the life and career of Boris Smolar, a journalist and editor-in-chief of the Jewish Telegraphic Agency and an author of children's books.Gift of Leivy Smolar
Signal transduction to the permeability transition pore
No full textThe permeability transition pore (PTP) is an inner mitochondrial membrane channel that has been thoroughly characterized functionally, yet remains an elusive molecular entity. The best characterized PTP-regulatory component, cyclophilin (CyP) D, is a matrix protein that favors pore opening. CyP inhibitors, CyP-D null animals, and in situ PTP readouts have established the role of PTP as an effector mechanism of cell death, and the growing definition of PTP signalling mechanisms. This review briefly covers the functional features of the PTP and the role played by its dysregulation in disease pathogenesis. Recent progress on PTP modulation by kinase/phosphatase signal transduction
is discussed, with specific emphasis on hexokinase and on the Akt-ERK-GSK3 axis, which might modulate the PTP through CyP-D phosphorylation
Long-Run Development and the New Cultural Economics
No full textThis paper reviews recent economics literature on culture, with an emphasis on its relation to the field of long-run growth and development. It examines the key issues debated in the new cultural economics: causal effects of culture on economic outcomes, the origins and social costs of culture, as well as cultural transmission, persistence, and change. Some of these topics are illustrated in application to the economic analysis of envy-related culture
Making Enterprise Information Systems Resilient Against Disruptive Events: A Conceptual View
No full textEnterprise Information Systems (EIS) are designed to deal with normal variability in their inputs and data. Empowered by CONTEXT-AWARENESS, some EIS even count on sensors and/or data analytics for capturing changes outside of the system. Nevertheless, context-awareness would often fail when EIS are affected by (large-scale) disruptive events, such as disasters, virus outbreaks, or military conflicts. Hence, in the current paper, we take a step forward, by considering context-awareness for disruptive events. We combine context-awareness with risk management techniques, such as FMECA and FTA, that are useful for defining and mitigating risk events. To avoid having to define the likelihood for such very-low-probability disruptive risks, we use CONSEQUENCE-BASED RISK MANAGEMENT rather than traditional risk management. We augment this approach with the context-awareness paradigm, delivering a contribution that is two-fold: (i) We propose context-awareness-related measures and consequence-based-risk-management-related measures, to address disruptive events; (ii) We reflect this in a method featuring the application of context-awareness and risk management for designing robust and resilient EIS.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Policy Analysi
Illustrated book of fiction, Boris Vian-Froth on the Daydream
No full textBakalářká práce Ilustrovaná kniha beletrie se v praktické části zaměřuje na koncepci ilustrací a jejich následnou kresbu pro knihu Pěna dní autora Borise Viana. V teoretické části práce je detailně sledován proces vzniku od prvotních idejí až po hlavní koncepční řešení celku. Text zde dále zkoumá formu díla skrze technologický proces výroby až po konečnou realizaci knihy a dalšího příslušenství, které kniha nabízí. Krom procesu vzniku a tvorby text obsahuje také kontextové srovnání, autorčinu osobní výpověď a zhodnocení výsledného díla.OVUObhájenoBachelor´s thesis Illustrated book of fiction in it´s practical part is aimed at conception of illustrations with following realisation of drawings for the book Froth on the Daydream by Boris Vian. In theoretical part the creation process of the book is observed there closely from the first ideas up to whole piece solution. Next part of the thesis is focused on technological process of manufacturing and final realisation of the book and it´s other accessories. Also the context comparison is described there as well as personal statement of the author and evaluation of the final work
Enforcing context-awareness and privacy-by-design in the specification of information systems
No full textNetworked physical devices, vehicles, home appliances, and other items embedded with electronics, software, sensors, actuators, and connectivity, allow for run-time acquisition of user data. This in turn can enable information systems which capture the “current” user state and act accordingly. The use of this data would result in context-aware applications that get fueled by user data (and environmental data) to adapt their behavior. Yet the use of data is often restricted by privacy regulations and norms; for example, the location of a person cannot be shared without given consent. In this paper we propose a design approach that allows for weaving context-awareness and privacy-by-design into the specification of information systems. This is to be done since the very early stages of the software development, while the enterprise needs are captured (and understood) and the software features are specified on that basis. In addition to taking into account context-awareness and privacy-sensitivity these two aspects will be balanced, especially if they are conflicting. The presented approach extends the “Software Derived from Business Components” (SDBC) approach. We partially demonstrate our proposed way of modeling, by means of a case example featuring land border security. Our proposed way of modeling would allow developers to smoothly reflect context and privacy features in the application design, supported by methodological guidelines that span over the enterprise modeling and software specification. Those features are captured as technology-independent societal demands and are in the end reflected in technology-specific (software) solutions. Traceability between the two is possible as well as re-use of modeling constructs.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Information and Communication Technolog
Biblical reception in rock-poetry: Boris Grebenshchikov
No full textThe article is devoted to the problem of biblical reception in Russian rock-poetry. Having analyzed Boris Grebenshikov’s interviews and texts, the author highlights his specific strategies for «alien» words interiorization. The article tells about biblical code and mechanisms of biblical text interiorization in Boris Grebenshchikov rock-poetry. The originality of individual creative manner of the rock-poet is revealed through the aspect of poetics under consideration
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