1,721,090 research outputs found
Isolation and subfractionation of mitochondria from animal cells and tissue culture lines
No full textA modular converter for converting the electric power produced by aerogenerators, and a wind-power plant that uses said converter
No full textA novel modular converter for high power aerogenerator
Convertitore modulare della potenza elettrica prodotta da generatori eolici e centrale eolica impiegante lo stesso
No full textDepositato il 30/04/200
Determination of mitochondrial number and function
No full textMitochondria transplantation is considered a potential therapeutic method that can be used in diseases related to mitochondrial dysfunction, such as neurodegenerative diseases, toxic lesions, ischemia, and cardiovascular diseases. To achieve successful transplantation, whether in vivo or in vitro, it is of paramount importance to accurately determine the number and quality of isolated mitochondria. The structural integrity of isolated mitochondria must be carefully evaluated, as it directly correlates with the organelles' ability to maintain transmembrane potential and thus promote their bioenergetic function by effectively synthesizing ATP
Serum amyloid A: Biology and clinical application [La siero amiloide A: Biologia ed applicazioni cliniche]
No full textSperm motility evaluation according to WHO VI edition: moving forward turning back?
Full text link“WHO Laboratory Manual for the Examination and Processing of Human Semen” was frst published in 1980 and was updated four times in the last 30 years (1987, 1992, 1999, 2010) [1]. Considerable eforts were made to translate the manual in numerous languages (in Italy it was edited by the Italian Society of Andrology and Sexual Medicine—SIAMS), allowing it to mark a level of standardization necessary for human semen analysis to improve both the overall quality and the comparability of results. This year the sixth edition of the WHO Manual is being published [2]. The 2021 version provides implementation of new topics of interest and several updates in the methodological descriptions. Briefy, this new edition covers semen analysis, spermatozoa preparation and cryopreservation and quality control. Procedures related to semen analysis include basic examinations (routine procedures for the evaluation of sperm parameters), second level analyses (extended semen examinations required in specifc conditions), research analyses (advanced examinations, not yet considered for laboratory routine use)
A particular case of AML patient with the polymorphism G105G (rs11554137) and the missense mutation R132C in IDH1 gene
No full textPolymorphic cytosine-adenine-guanine repeat length of androgen receptor gene and gender incongruence in trans women: a systematic review and meta-analysis of case-control studies
No full textIntroduction: It has been hypothesized that gender incongruence in transgender women could result from an antenatal impaired androgen activity on the developing brain. As the length of polymorphic cytosine-adenine-guanine (CAG) repeat sequences in the androgen receptor (AR) gene is inversely correlated with AR transcriptional activity, some studies explored a possible association between long CAG repeats and gender incongruence in trangender women. Yet results remain inconclusive. Aim: To systematically evaluate whether a difference exists in the length of AR CAG repeat sequences between trans women and men without gender incongruence. Methods: A thorough search of Medline, Scopus, Cochrane Library, Web of Science, and CINAHL databases was carried out to identify suitable case-control studies. Methodological quality of the included articles was assessed using the Newcastle-Ottawa Scale. In the absence of between-studies heterogeneity, as assessed by the Cochrane's Q and I2 tests, standardized mean differences (SMDs) in the length of AR CAG repeats were combined using a fixed effect model. Funnel plot and trim-and-fill analysis were used to assess publication bias. Main Outcome Measure: The association of gender incongruence in transgender women with longer length of AR CAG repeat sequences was evaluated by calculating pooled standardized mean difference with 95% confidence interval (CI). Results: 5 studies included in the quantitative analysis collectively provided information on 795 trans women and 1,355 control men. At the overall estimate, the MtF group exhibited a significantly longer length of AR CAG repeat sequences (pooled standardized mean difference: 0.13, 95% CI: 0.04 to 0.22; P = 0.005; I2 = 0%, Pfor heterogeneity = 0.51). Sensitivity analysis demonstrated the high stability of the result. Funnel plot revealed a possible publication bias, and the trim-and-fill test detected 2 putative missing studies. Nevertheless, the significant association persisted even when pooled estimate was adjusted for publication bias. Clinical Implications: These findings could suggest a contribution of a genetically mediated impairment in androgen signaling in development of gender incongruence for transgender women. Strength & Limitations: This is the first meta-analysis exploring the relationship between AR CAG repeat polymorphism and gender incongruence. However, interactions with other functional genetic variants were not explored, and caution should be exercised when generalizing these results because of the possible variability in the distribution of CAG repeats among different populations and ethnic groups. Conclusion: Trans woman population exhibits significantly longer polymorphic CAG repeat sequences in the AR gene. Further studies are warranted to elucidate whether, how and to what extent multiple functional variants in sex hormone signaling genes could be associated with gender incongruence/dysphoria. D'Andrea S, Pallotti F, Senofonte G, et al. Polymorphic Cytosine-Adenine-Guanine Repeat Length of Androgen Receptor Gene and Gender Incongruence in Trans Women: A Systematic Review and Meta-Analysis of Case-Control Studies. J Sex Med 2020;XX:XXX–XXX
Isolation process for mitochondrial transplantation
No full textMitochondrial transplantation is an innovative therapeutic approach for the treatment of mitochondrial dysfunction associated with a variety of metabolic, neurodegenerative and cardiovascular diseases. In this procedure, healthy mitochondria are isolated and transferred into damaged or dysfunctional cells to restore normal cellular bioenergetics. Recent advances have shown the feasibility of mitochondrial transplantation in preclinical and clinical settings, demonstrating its potential to improve outcomes in conditions such as myocardial infarction, neurodegenerative disorders, and acute kidney injury. In this contest, the isolation method for mitochondrial transplantation is crucial. High quality, pure mitochondria are essential to ensure that the transplanted organelles function correctly and integrate well into the recipient cells. In addition, efficient isolation techniques are required to obtain a sufficient number of mitochondria for transplantation. Low yield methods may not provide enough mitochondria for effective treatment. Several isolation techniques are currently used, including differential centrifugation, density gradient centrifugation and fractionated mitochondrial magnetic separation. Each method has its own advantages and limitations, and the choice of method often depends on the specific requirements of the application, such as the type of cells targeted and the disease to be treated. A key factor to consider when choosing the isolation method is the isolation time. Rapid isolation methods are preferred for the isolation of mitochondria from tissue biopsies. This review summarizes the current state of research on mitochondrial transplantation, highlights the main methods for isolating mitochondria and discusses their potential clinical applications in the field of regenerative medicine
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