1,180 research outputs found

    Deriving Regression Lines Without Calculus

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    This article, C.W. Puritz of the Royal Grammar School - High Wycombe, describes a method to calculate the least squares line algebraically. First, the author uses a numeric example, which uses calculus, then describes a simpler algebraic method. This is a great lesson plan for those instructors attempting to teach this method with having students with prior knowledge of calculus. The author uses a step by step approach to help best describe both the technique and subsequent ways to best teach this method

    Development and optimisation of a duplex real-time reverse transcription quantitative PCR assay targeting the VP7 and NS2 genes of African horse sickness virus

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    Nucleotide sequences of 52 South African isolates of African horse sickness virus (AHSV) collected during 2004–2005 and including viruses of all nine AHSV serotypes, were used to design and develop a duplex real-time reverse transcription quantitative PCR (RT-PCR) assay targeting the VP7 (S8) and NS2 (S9) genes of AHSV. The assay was optimized for detection of AHSV in fresh and frozen blood of naturally infected horses. Assay performance was enhanced using random hexamers rather than gene-specific primers for RT, and with denaturation of double-stranded RNA in the presence of random hexamers. The assay was efficient with a linear range of at least five orders of magnitude. The analytical sensitivity of the assay was 132 copies of the target genes (4125 copies per ml of blood), and the assay was at least 10-fold more sensitive than virus isolation on BHK-21 cells. The assay was also highly specific because it did not detect related orbiviruses, such as bluetongue and equine encephalosis viruses.ID: S0166093410000893; M3: Article; Accession Number: S0166093410000893; Author: M. Quan (a, b, ⁎); Author: C.W. Lourens (a, b); Author: N.J. MacLachlan (c); Author: I.A. Gardner (d); Author: A.J. Guthrie (a); Affiliation: Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa; Affiliation: Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa; Affiliation: Equine Viral Disease Laboratory, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA; Affiliation: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA; Keyword: African horse sickness virus; Keyword: Real-time quantitative RT-PCR; Keyword: VP7 gene; Keyword: NS2 gene; Keyword: Duplex; Number of Pages: 8; Language: English

    Nota, betreffende berekeningen omtrent rivierverbeteringen: opgemaakt door den ingenieur van den Rijkswaterstaat C.W. Lely

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    Hydraulische basis voor de normalisatiewerken van de grote rivieren. Berekingen van optimale breedte en diepte

    Onderzoek naar signaalgeneratie met behulp van fasedraaiers ten behoeve van reflectie-compensatie in een F.M.-C.W. radarsysteem

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    Voor onderzoek aan de troposfeer wordt gebruik gemaakt van een F.M.-C.W. radarsysteem. Een aspect van het radarsysteem waarnaar nog onderzoek gewenst is, is de onderdrukking van de overspraak van zender naar ontvanger, en van sterke reflecties aan nabije obstakels: deze signalen houden nl. het gevaar in van oversturing van de ontvanger. Dit aspect is met name van belang voor een mogelijk nog te realiseren F.M. -C.V. radarsysteem met één antenne…Applied SciencesElectrotechniekTelecommunicatie- en Verkeersbegeleidingssysteme

    A Fourier-Based Valuation Method for Bermudan and Barrier Options under Heston’s Model

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    We develop an efficient Fourier-based numerical method for pricing Bermudan and discretely monitored barrier options under the Heston stochastic volatility model. The two-dimensional pricing problem is dealt with by a combination of a Fourier cosine series expansion, as in [F. Fang and C.W. Oosterlee, SIAM J. Sci. Comput., 31 (2008), pp. 826–848, F. Fang and C. W. Oosterlee, Numer. Math., 114 (2009), pp. 27–62], and high-order quadrature rules in the other dimension. Error analysis and experiments confirm a fast error convergence.Delft Institute of Applied MathematicsElectrical Engineering, Mathematics and Computer Scienc
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