453 research outputs found
Introduction
No full textCollected by Merlyn B. Page Told by Merlyn B. Page and
and James R. Hayes James R. Hayes
Transcribed by Nathaniel Lucy Fayetteville, Arkansas
September 28, 1958
Reel 277
Introduction
Merlyn B. Page: This is Merlyn Brown Page
James R. Hayes: and Jimmy Hayes.
Page: We're at the house of Miss Oleavia Houser. The date is
September 28, 1958. The first song is
Oleavia Houser: "Black Jack Davy".Funding for digitization provided by the Arkansas Humanities Council and the Happy Hollow Foundation
Merlin conformations.
No full text(A) Fractionation of purified Merlin-NL (inset) on an SEC 650 gel filtration column, aliquots from 0.2 ml fractions were assayed for NanoLuc activity and plotted with the elution volume. The approximate Stokes radius of the peaks was calculated relative to the mobility of standards of known molecular weight and Stokes radius. (S2 Fig). The large peak at 12.4 ml has an approximate Stokes radius of 5.0 nm and the smaller peak at 15 ml has an approximate Stokes radius of 2.1 nm. (B) SDS-PAGE with BSA standards and an aliquot of the purified Merlin-NL stained for total protein (top). Fractions composing peaks 1 and 2 were immunoblotted and probed with antibodies for either total Merlin or P-S518-Merlin (bottom). (C). Gel fractionation of lysates from cells co-transfected with Merlin-NL and Merlin-GFP. NanoLuc activity from the fractions is shown as blue circles and depicted on the right-hand y-axis. Dimers represented by NanoLuc activity from GFP pulldowns of the fractions is shown as green squares and depicted on the left-hand y-axis. (D) Gel fractionation of purified Merlin-NL at in hypotonic (50 mM NaCl), isotonic (150 mM NaCl) and hypertonic (500 mM NaCl) conditions. (E) Images of dimerization assays dimerization assays performed at 50 mM, 75 mM, 150 mM, 300 mM, and 600 mM NaCl. The GFP-NanoLuc fusion protein used to control for the effects of salt on luminescence. (F) Quantitation of the dimerization data presented as a mean of triplicate binding reactions with standard deviation and expressed as a percentage of the peak value. (G) Immunoblot assays of iHSC-1λ immortalized Schwann cell lysates fractionated by gel filtration and probed for either total Merlin (top) or P-S518-Merlin (bottom).</p
Merlin dimerizes via the FERM domain.
No full text(A). A diagram depicting the dimerization assay using Merlin with either GFP or NanoLuc fused to its C-terminus. (B). A schematic diagram depicting the full-length Merlin (aa 1–595, WT) and the C-terminal deletion mutant (FH, aa 1–511), the FERM domain deletion mutant (HC, aa 315–595) and the dual FERM and CTD deletion (H, aa 315–511). (C). SDS-PAGE gels stained for total protein to access the quantity and purity of isolated probes for Merlin-NL (top) and GFP “bait” proteins (bottom) for GFP, Merlin-GFP, Merlin-FH-GFP, Merlin-HC-GFP and Merlin-H-GFP. (D). Merlin dimerization data quantified on a plate reader and presented as the bound luciferase normalized to 10% of the unbound input NL probe. The data is a mean of triplicate binding reactions with standard deviation and expressed as a percentage of the wild-type Merlin. Inset: an image of the light emitted from Merlin-NanoLuc bound to Merlin and Merlin deletion mutant-GFP bound magnetic beads, performed in triplicate, and dispensed into the wells of a 96-well plate (left). (E). A schematic diagram depicting the N- and C-terminal GFP fused “bait” and the N- and C-terminal fused Merlin-NanoLuc “Probe” constructs used in the BRET assays. (F). Triplicate Merlin dimerization assays for the N-and C-terminal Merlin constructs. The data is a ratio of bound to unbound NL probe, normalized to GFP fluorescence, (mean of triplicate binding reactions with standard deviation and expressed as a percentage of the peak value). (G). Emission spectrum from 400 nm to 600 nm of Merlin dimerization assays normalized to the 450 nm peak. The BRET emission peak at 510 nm is indicated by the arrow.</p
CD43 signaling targets the Merlin pathway.
No full text<p>A549 clones containing the empty pSuper (pSup) vector or expressing the CD43 specific RNAi (RNAi) were grown to confluence (t=0) and further cultured for the indicated time points. At each time total cell extracts were prepared and the phosphorylation levels of STAT3 (p-STAT3), AKT (p-AKT), and GSK3β (p-GSK3) (<b>A</b>) as well as total Merlin levels (<b>B</b>) were determined by immunoblot, using specific antibodies. ERK protein levels were determined as loading control. <b>C</b>) Total cell extracts from A549 lung tumor cells cultured to confluence (t=0) or further cultured for 48 hrs in the absence (-) or presence of 20 μM LY294002 were resolved by SDS-PAGE and Merlin protein levels (Merlin) or phosphorylated AKT (pAKT) were evaluated by immunoblot with specific antibodies. ERK protein levels were used as loading control. <b>D</b>) A549 clones containing the empty pSuper (pSup) vector or expressing the CD43 specific RNAi (RNAi) were grown to confluence (t=0) and cultures were maintained for the indicated time points. Total cell extracts were prepared and the phosphorylation levels of YAP (p-YAP), ERK protein levels (loading control) were determined by immunoblot using specific antibodies. <b>E</b>) A549 clones expressing the empty pSuper (pSup) vector or the CD43 specific RNAi (RNAi) were grown to confluence and cultures were maintained in the absence or presence of 20 μM LY294002 (+LY) for 48 hrs. Cells were then harvested and counted. The graph represents the average cell number ± SD of three independent experiments using at least three independent clones. *p < 0.05, **p < 0.01 vs pSup.</p
"Thank me therefore": Social Prestige, Probity and Self-determination of Nymue's character in Malory's "Le Morte Darthur"
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Measurement of disorder in non-periodic sequences
No full textAn information theoretic measure is introduced to compare the disorder in non-periodic sequences. It is shown that the measure correctly distinguishes quasiperiodic and aperiodic sequences which have been deduced from earlier studies using diffraction patterns, although it is often necessary to use a set of measures, depending on the order of the source used. The particular sequences studied are the Thue-Morse sequence and the generalizations of the golden mean sequence commonly studied in connection with quasicrystals.PT: J; CR: ALI MK, 1988, PHYS REV B, V38, P7091 BOMBIERI E, 1986, J PHYS-PARIS, V47, P19 BOMBIERI E, 1987, CONT MATH, V64, P241 BURROWS BL, 1989, INT J MATH ED SCI TE, V20, P913 CHENG Z, 1988, PHYS REV B, V37, P4375 GUMBS G, 1988, J PHYS A, V21, L517 GUMBS G, 1988, PHYS REV LETT, V60, P1081 GUMBS G, 1989, J PHYS A-MATH GEN, V22, P951 HAMMING RW, 1980, CODING INFORMATION T HOLZER M, 1988, PHYS REV B, V38, P1709 HOLZER M, 1988, PHYS REV B, V38, P5756 KOLAR M, 1990, PHYS REV B, V41, P7108 KOLAR M, 1991, PHYS REV B, V43, P1034 MA HR, 1988, J PHYS C SOLID STATE, V21, P4311 MERLIN R, 1985, PHYS REV LETT, V55, P1768 MORSE M, 1921, AM J MATH, V43, P35 MORSE M, 1921, T AM MATH SOC, V22, P84 NIU Q, 1986, PHYS REV LETT, V57, P2057 PENROSE R, 1974, B I MATH APPL, V10, P266 QIN MG, 1990, J PHYS-CONDENS MAT, V2, P1059 RIKLUND R, 1987, INT J MOD PHYS B, V1, P121 SHANNON CE, 1949, MATH THEORY COMMUNIC SHECHTMAN D, 1984, PHYS REV LETT, V53, P1951 THUE A, 1906, NORSKE VID SELSK IMN, V7, P1 THUE A, 1912, NORSKE VID SELSK IMN, V1, P1; NR: 25; TC: 13; J9: J PHYS-A-MATH GEN; PG: 9; GA: GC466Source type: Electronic(1
Genomewide linkage scan of schizophrenia in a large multicenter pedigree sample using single nucleotide polymorphisms
No full textA genomewide linkage scan was carried out in eight clinical samples of informative schizophrenia families. After all quality control checks, the analysis of 707 European-ancestry families included 1615 affected and 1602 unaffected genotyped individuals, and the analysis of all 807 families included 1900 affected and 1839 unaffected individuals. Multipoint linkage analysis with correction for marker-marker linkage disequilibrium was carried out with 5861 single nucleotide polymorphisms (SNPs; Illumina version 4.0 linkage map). Suggestive evidence for linkage ( European families) was observed on chromosomes 8p21, 8q24.1, 9q34 and 12q24.1 in nonparametric and/or parametric analyses. In a logistic regression allele-sharing analysis of linkage allowing for intersite heterogeneity, genomewide significant evidence for linkage was observed on chromosome 10p12. Significant heterogeneity was also observed on chromosome 22q11.1. Evidence for linkage across family sets and analyses was most consistent on chromosome 8p21, with a one-LOD support interval that does not include the candidate gene NRG1, suggesting that one or more other susceptibility loci might exist in the region. In this era of genomewide association and deep resequencing studies, consensus linkage regions deserve continued attention, given that linkage signals can be produced by many types of genomic variation, including any combination of multiple common or rare SNPs or copy number variants in a region. Molecular Psychiatry (2009) 14, 786-795; doi:10.1038/mp.2009.11; published online 17 February 2009</p
Coordinated sleeping for beaconless 802.15.4-based multihop networks
No full textPaper presented at the 1st International Conference on Sensor Systems and Software, September 7-9, 2009 - Pisa, ItalyThe last few years have seen a wide adoption of the IEEE 802.15.4 MAC/PHY standard for low-power communication between
wireless sensor nodes. Within this work we study some fundamental drawbacks of the 802.15.4 specifications for multihop network deployments, which adversely affect the delivery rate and efficient node energy
consumption. These issues are rectified by investigating a timezone-based scheduling, V-Route, that builds on 802.15.4 beaconless mode to enable both a synchronized sleep scheduling and a bidirectional communication
between nodes in the sensor network and the PAN coordinator. The contributions of V-Route are threefold: (1) mitigate collisions, (2) enable packet routing and (3) provide energy saving in a multihop context, while maintaining the full compliancy with the 802.15.4 standard. We present a performance evaluation on energy consumption and latency with real
experiments on Philips AquisGrain sensor nodes. Enhancing 802.15.4-based multi-hop networks with V-Route yields energy reduction ranging from 27.3% to 85.3%, according to the required end-to-end latency.Science Foundation IrelandConference detailshttp://www.s-cubeconference.org/2009
The Data Centre Nature and Landscape (DNL): Service Oriented Architecture, Metadata Standards and Semantic Technologies in an Environmental Information System
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