1,928 research outputs found
ISR1, A TRANSPOSABLE DNA-SEQUENCE RESIDENT IN RHIZOBIUM CLASS-IV STRAINS, SHOWS STRUCTURAL CHARACTERISTICS OF CLASSICAL INSERTION ELEMENTS
No full textPRIEFER UB, Kalinowski J, RUGER B, HEUMANN W, Pühler A. ISR1, A TRANSPOSABLE DNA-SEQUENCE RESIDENT IN RHIZOBIUM CLASS-IV STRAINS, SHOWS STRUCTURAL CHARACTERISTICS OF CLASSICAL INSERTION ELEMENTS. PLASMID. 1989;21(2):120-128
NUCLEOTIDE-SEQUENCE OF A 24,206-BASE-PAIR DNA FRAGMENT CARRYING THE ENTIRE NITROGEN-FIXATION GENE-CLUSTER OF KLEBSIELLA-PNEUMONIAE
No full textArnold W, RUMP A, KLIPP W, PRIEFER UB, Pühler A. NUCLEOTIDE-SEQUENCE OF A 24,206-BASE-PAIR DNA FRAGMENT CARRYING THE ENTIRE NITROGEN-FIXATION GENE-CLUSTER OF KLEBSIELLA-PNEUMONIAE. JOURNAL OF MOLECULAR BIOLOGY. 1988;203(3):715-738
Signal molecules and regulatory components in the Rhizobium-legume symbiosis.
No full textPriefer UB, Patschkowski T, Schlüter A. Signal molecules and regulatory components in the Rhizobium-legume symbiosis. Endocytobiosis and Cell Research. 1998;12(3):201-202
Identifying genes suitable for constructing pH and salt tolerant Rhizobium inoculants for improving French bean cultivation under semiarid conditions
No full textBoesten B, Schlüter A, Prell J, Krämer M, Lipka V, Priefer UB. Identifying genes suitable for constructing pH and salt tolerant Rhizobium inoculants for improving French bean cultivation under semiarid conditions. In: Olivares J, Palomares AJ, eds. Proceedings of the Fourth European Nitrogen Fixation Conference. 2000: 319
Extension of the host range of Escherichia coli vectors by incorporation of RSF1010 replication and mobilization functions
No full textPriefer UB, Simon R, Pühler A. Extension of the host range of Escherichia coli vectors by incorporation of RSF1010 replication and mobilization functions. JOURNAL OF BACTERIOLOGY. 1985;163(1):324-330
ISR1 - AN INSERTION ELEMENT ISOLATED FROM THE SOIL BACTERIUM RHIZOBIUM-LUPINI
No full textPriefer UB, Burkardt HJ, Klipp W, Pühler A. ISR1 - AN INSERTION ELEMENT ISOLATED FROM THE SOIL BACTERIUM RHIZOBIUM-LUPINI. COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY. 1981;45 Pt 1:87-91
Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame
No full textGrönger P, Manian SS, Reiländer H, O´Connell M, Priefer UB, Pühler A. Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame. Nucleic Acids Research. 1987;15(1):31-49.By hybridization and heteroduplex studies the fixABC and nifA genes of the Rhizobium leguminosarum symbiotic plasmid pRL6JI have been identified. DNA sequencing of the region containing nifA showed an open reading frame of 1557 bp encoding a protein of 56,178 D. Based on sequence homology, this ORF was confirmed to correspond to the nifA gene. Comparison of three nifA proteins (Klebsiella pneumoniae, Rhizobium meliloti, Rhizobium leguminosarum) revealed only a weak relationship in their N-terminal regions, whereas the C-terminal parts exhibited strong homology. Sequence analysis also showed that the R. leguminosarum nifA gene is followed by nifB and preceded by fixC with an open reading frame inserted in between. This novel ORF of 294 bp was found to be highly conserved also in R. meliloti. No known promoter and termination signals could be defined on the sequenced R. leguminosarum fragment
SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION
No full textSTEINMANN D, WIGGERICH HG, KLAUKE B, SCHRAMM U, Pühler A, PRIEFER UB. SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 1993;40(2-3):356-360.A system allowing transposon mutagenesis of cloned DNA fragments in Escherichia coil with Tn4431, which carries the promotorless luciferase (lux) operon of Vibrio fischeri, has been developed. The transposon delivery plasmid, pDS1, based on an IncF replicon, is thermosensitive in replication and mobilizable to many Gram-negative bacteria. We used pDS1 for Tn4431-saturation mutagenesis of a 10-kb DNA fragment of Xanthomonas campestris pv. campestris (X.c.c.) in E. coli and showed that the expression of the lux operon was dependent on orientation and location of the transposon. Transfer of a specific Tn4431 insertion to X.c.c. allowed the determination of the bioluminescence phenotype in planta
A homolog of the Rhizobium meliloti nitrogen fixation gene fixN is involved in the production of a microaerobically induced oxidase activity in the phytopathogenic bacterium Agrobacterium tumefacience
No full textSchlüter A, Rüberg S, Krämer M, Weidner S, Priefer UB. A homolog of the Rhizobium meliloti nitrogen fixation gene fixN is involved in the production of a microaerobically induced oxidase activity in the phytopathogenic bacterium Agrobacterium tumefacience. MOLECULAR & GENERAL GENETICS. 1995;247(2):206-215
THE RHIZOBIUM-LEGUMINOSARUM FNRN PROTEIN IS FUNCTIONALLY SIMILAR TO ESCHERICHIA-COLI FNR AND PROMOTES HETEROLOGOUS OXYGEN-DEPENDENT ACTIVATION OF TRANSCRIPTION
No full textSchlüter A, Patschkowski T, UNDEN G, PRIEFER UB. THE RHIZOBIUM-LEGUMINOSARUM FNRN PROTEIN IS FUNCTIONALLY SIMILAR TO ESCHERICHIA-COLI FNR AND PROMOTES HETEROLOGOUS OXYGEN-DEPENDENT ACTIVATION OF TRANSCRIPTION. MOLECULAR MICROBIOLOGY. 1992;6(22):3395-3404.An open reading frame from Rhizobium leguminosarum bv. viciae strain VF39, previously identified and found to be similar to Escherichia coli fnr and Rhizobium meliloti fixK (orf240, thereafter called fnrN), was further analysed. Analysis of the expression of an fnrN-lacZ transcriptional fusion revealed that fnrN is preferentially expressed under oxygen limitation. Using R. meliloti fixN-lacZ fusions it was shown that the fnrN gene product only mediates transcriptional activation under microaerobiosis, indicating that the FnrN protein responds, directly or indirectly, to oxygen. Plasmids which expressed fnrN under the control of an E. coli promoter were able to complement an E. coli fnr mutant with respect to anaerobic growth on nitrate but not fumarate, and to promote anaerobic but not aerobic activation of the Fnr-dependent E. coli genes narGHJI, nirB and fdnGHI coding for nitrate reductase, NADH-dependent nitrite reductase and formate dehydrogenase-N, respectively. Fumarate and DMSO reductase activities were not induced by FnrN. The E. coli fnr gene substituted for fnrN in oxygen-regulated transcription of nirB- and fixN-lacZ fusions in R. leguminosarum. The results indicate that Fnr and FnrN are functionally very similar and share a common mode of oxygen-dependent transcriptional activation. From hybridizaton studies, it appeared that fnrN-like genes are present in a number of different R. leguminosarum strains
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