1,720,998 research outputs found
In situ hybridization by scanning electron microscopy for painting, centromeric, and YAC localization
No full textThe hybridization site of a DNA probe was detected using a scanning electron microscope (SEM), modifying the standard in situ hybridization (ISH) method.The experiments were performed on human metaphases obtained from lymphocyte cultures of human peripheral blood. The libraries and probes used were: 1-chromosome library for the painting of chromosome 1 (wcp 1), an alphoid centromere-specific probe of chromosome 8 (pZ8.4), and the yeast artificial chromosome (YAC) 964-C10 mapped at band p13 on chromosome 12. These probes were labeled by nick translation with biotin and displayed with a gold-conjugated anti biotin goat antibody. The gold signal was amplified by silver enhancement. The chromatides appeared as packages of thin filaments 120 nm high; some of them collapsed, probably due to ISH procedures. All the probes were clearly detected as small gold particles grouped on the surface of the target chromosomes and chromosome sites. Thus, this procedure is useful to clarify the positional relationship between the chromatin filaments and the probe
Improving the definition of the structure of the isochromosome i(7)(q10) in Shwachman-Diamond Syndrome
No full textL’array-CGH per monitorare anomalie cromosomiche sbilanciate nelle malattie displastiche/neoplastiche puo’ rilevare fino all’8% di cellule anomale
No full textComparative genomic hybridization su microarray (aCGH) in sindrome di Shwachman (OMIM 260400) e rischio di mielodisplasia: al di là della citogenetica standard
No full textArray-CGH used to monitor dysplastic/neoplastic disorders with unbalanced chromosome anomalies may detect as low as 8% abnormal cells
No full textAplasia midollare da perdita del gene RUNX1 acquisita a causa di un riarrangiamento strutturale clonale del cromosoma 21
No full textSindrome di shwachman: anomalie cromosomiche clonali nel midollo osseo e differenziamento della linea mieloide
No full textLa sindrome da aneuploidia variegata a mosaico (MVA) associata a neutropenia come condizione predisponente a displasia e neoplasia ematologia
No full textAcquiring an "inherited" thrombocitopenia: clonal chromosome anomalies affecting FLI1 mimic inherited thrombocitopenia of Paris-Trousseau type
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