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    Fas in human semen

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    The authors state that sperm apoptosis is a useful indicator of male infertility in the case of varicocele, while being unable to detect Fas and Fas-L on sperm cells. At the same time, they also claim that the presence of Fas is a debated issue. We demonstrated beyond any reasonable doubt that Fas is not present in human sperm and clarified that detections of Fas from other authors were due to interference with leukocytes in seminal samples. To prove this, we performed semen analysis through a multiparameter flow cytometry method that excluded leukocytes from the sperm population

    Serum IgA and IgG antibodies to alpha-gliadin: comparison between two ELISA methods

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    We have developed two ELISA methods, i.e., enzyme immunoassay (EIA) and fluorescence immunoassay (FIA), for the semiquantitative detection of specific IgA and IgG antibodies directed against α-gliadin. The tests differ only for the enzyme substrate and, when optimized, could be used in large routine screening of celiac disease. Several serum samples from patients with celiac disease and gastrointestinal disorders as well as from control subjects were tested. Both methods gave good correlation with clinical data, were easily performed and had same specificity features, while FIA proved to be more sensitive
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