1,721,082 research outputs found
ELISA: a new tool for the large scale detection of Plum bark necrosis stem pitting-associated virus
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Interlaboratoty validation of molecular and serological diagnosis of Xylella fastidiosa stris CoDiRO in susceptible host plants.
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A NEW HERBACEOUS HOST OF CITRUS LEAF BLOTCH VIRUS
No full textCitrus leaf blotch virus (CLBV), the type species of
the putative new genus Citrivirus, causes a bud union
disorder of Nagami kumquat and Calamondin scions
grafted on trifoliate rootstocks. This virus was successfully
transmitted to Nicotiana cavicola using leaf extracts
of infected Nagami kumquat and Etrog citron, thus
widening its herbaceous host range. The infection was
latent but confirmed by the positive response of RTPCR
using virus-specific primers and by electron microscopy.
The positive transmission of CLBV to N. cavicola
should in principle facilitate laboratory investigations,
as it provides a new source of virus alternative to
and more manageable than citrus
A Xylella fastidiosa strain with unique Biology and phylogeny is associated with severe disease of olive in Southern Apulia
Full text linkProduzione di un anticorpo monoclonale specifico per il ceppo del virus della vaiolatura del susino isolato da ciliegio dolce (PPV-SwC).
No full textInterlaboratory validation of molecular and serological diagnosis of Xylella fastidiosa strain CoDiRO in susceptible host plants.
Full text linkFirst international proficiency testing for laboratory performance on Xylella fastidiosa
No full textA proficiency test (PT) to evaluate the performance of laboratories involved in molecular and serological detection of Xf was carried out in early 2017. Thirty-five laboratories from EU/non-EU Countries tested 4 different methods to purify DNA, conventional and qPCR assays, and 2 ELISA tests. The number of resultant positive agreement/negative agreement/positive deviation/negative deviation was used to determine the laboratory performance (i.e. accuracy 100%). The overall results showed that all laboratories were able to correctly diagnose Xf in the blind samples containing the highest Xf concentrations, whereas the performance of several laboratories was negatively affected by the lack of detection in the samples with the lowest concentrations, both through molecular and serological tests. Accuracy level of 100% (laboratory conformed to the PT) was successfully recovered in the majority of the laboratories performing qPCR assays on DNA purified using at least 2 of the 4 tested protocols. The use of automated platform ensured higher laboratory performance. As expected, results of the ELISA tests generated lower performance values in the majority of the laboratories, due to the lack of detection of positive samples containing the lowest the bacterial concentration. This study provides a good overview on the laboratory performance for the diagnostics currently used in the EPPO countries and indicate useful improvements that laboratories can adopt to achieve a better performanc
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