1,721,020 research outputs found
A putative ORF of 78aa, at the PET18 locus, is involved in conferring thermotolerance in Saccharomyces cerevisiae
No full textAbstrac
Transcriptional regulation of the human tumor suppressor p14ARF by E2F1, E2F2, E2F3 and SP1-like factors
Full text linkThe human ARF/INK4a locus encodes two cell cycle inhibitors, p16INK4a and p14ARF, by using separate promoters. A variety of mitogenic stimuli upregulate ARF but a direct modulation at the transcriptional level has been reported only for E2F-1. We show here that the ARF promoter is strongly responsive also to E2F2 and E2F3, thus providing a strong support to their suggested role in the induction of apoptosis. Through the usage of both deletion mutants and/or site-directed mutants, we surprisingly found that none of the four putative E2F consensus sites is strictly necessary for the upregulation of ARF expression, as a minimal deletion mutant, lacking all the putative E2F binding sites, is still transactivated by E2F. Moreover, our data suggest that the ARF promoter is regulated by E2F through both direct binding to the promoter sequences and indirectly, probably by Sp1-like factors
Saccharomyces cerevisiae multifunctional protein RAP1 binds to a conserved sequence in the Polyoma virus enhancer and is responsible for its transcriptional activity in yeast cells
No full textSaccharomyces cerevisiae multifunctional protein RAP1 binds to a conserved sequence in the Polyoma virus enhancer and is responsible for its transcriptional activity in yeast cells
No full textA peptide secreted by an intestinal isolate of Lactobacillus mucosae reduces TNFa-induced apoptosis in colorectal carcinoma HCT116 cells
No full textBacillus megaterium SF185 induces stress pathways and affects the cell cycle distribution of human intestinal epithelial cells
No full textThe interaction between the enteric microbiota and intestinal cells often involves signal molecules that affect both microbial behaviour and host responses. Examples of such signal molecules are the molecules secreted by bacteria that induce quorum sensing mechanisms in the producing microorganism and signal transduction pathways in the host cells. The pentapeptide competence and sporulation factor (CSF) of Bacillus subtilis is a well characterized quorum sensing factor that controls competence and spore formation in the producing bacterium and induces cytoprotective heat shock proteins in intestinal epithelial cells. We analysed several Bacillus strains isolated from human ileal biopsies of healthy volunteers and observed that some of them were unable to produce CSF but still able to act in a CSF-like fashion on model intestinal epithelial cells. One of those strains belonging to the Bacillus megaterium species secreted at least two factors with effects on intestinal HT29 cells: a peptide smaller than 3 kDa able to induce heat shock protein 27 (hsp27) and p38-MAPK, and a larger molecule able to induce protein kinase B (PKB/Akt) with a pro-proliferative effect
The HTL1 gene (YCR020W-b) of Saccharomyces cerevisiae is necessary for growth at 37 °C, and for the conservation of chromosome syability and fertlity
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